Fixation conditions affect the immunoreactivity of gustducin in rat vallate taste buds.

Q4 Medicine

Archives of histology and cytology Pub Date : 2010-01-01 DOI:10.1679/aohc.73.91

Maiko Haga, Sumio Yoshie

引用次数: 2

Abstract

This study aimed to examine gustducin immunoreactivities when adopting various immunostaining conditions in rat vallate taste buds. The occurrence and intensity of the immunoreactivities exhibited specific patterns in accordance with the fixation time. The immunoreactions were localized to only taste hairs, the upper part of the taste bud, after short fixation periods but then to the cell-body cytoplasm excluding the taste hairs after long fixation periods. These immunohistochemical data suggest that the staining protocols, especially the fixation time, cause discrepancies in gustducin immunoreactivities.

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固定条件对大鼠味蕾中gustducin的免疫反应性有影响。

本研究旨在探讨不同免疫染色条件下大鼠味蕾中gustducin的免疫反应性。免疫反应的发生和强度随固定时间的不同呈现出特定的模式。固定时间短时，免疫反应仅局限于味蕾上部的味毛，固定时间长时，免疫反应扩展到除味毛外的细胞质。这些免疫组织化学数据表明，染色方案，特别是固定时间，会导致gustducin免疫反应性的差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of histology and cytology 生物-细胞生物学

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期刊介绍： The Archives of Histology and Cytology provides prompt publication in English of original works on the histology and histochemistry of man and animals. The articles published are in principle restricted to studies on vertebrates, but investigations using invertebrates may be accepted when the intention and results present issues of common interest to vertebrate researchers. Pathological studies may also be accepted, if the observations and interpretations are deemed to contribute toward increasing knowledge of the normal features of the cells or tissues concerned. This journal will also publish reviews offering evaluations and critical interpretations of recent studies and theories.