Archives of Medical Science最新文献_第4页

Good efficacy achieved by telitacicept in treatment of systemic lupus erythematosus with alopecia areata. 替立替塞治疗系统性红斑狼疮合并斑秃取得良好疗效。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-30 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/188860

Fangling Yao, Shenyi Yu, Zheng Liao, Li Deng

引用次数: 0

Combretastatin A4 phosphate encapsulated in hyaluronic acid nanoparticles is highly cytotoxic to oral squamous cell carcinoma. 封装在透明质酸纳米颗粒中的磷酸考布他丁 A4 对口腔鳞状细胞癌具有很强的细胞毒性。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-30 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/189535

Chuanxi Sun, Ziqi Zhou, Fangqiang Liu, Hong Li, Zhe Liu

引用次数: 0

The mediating effect of depression on the association between lung disease and cardiovascular health. 抑郁症对肺病与心血管健康之间关系的中介效应。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-30 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/189973

Feng Chen, Hao Lin, Yuansi Zhang, Yu Zhang, Enhui Yang

引用次数: 0

LINC00847 drives pancreatic cancer progression by targeting the miR-455-3p/HDAC4 axis. LINC00847 通过靶向 miR-455-3p/HDAC4 轴驱动胰腺癌进展。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-30 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/171672

Shunxin Hao, Zhi Yao, Yifeng Liu

{"title":"LINC00847 drives pancreatic cancer progression by targeting the miR-455-3p/HDAC4 axis.","authors":"Shunxin Hao, Zhi Yao, Yifeng Liu","doi":"10.5114/aoms/171672","DOIUrl":"https://doi.org/10.5114/aoms/171672","url":null,"abstract":"Introduction: Pancreatic cancer (PC) is a common malignant tumor of the digestive system, posing a serious threat to the life of patients. This study aims to investigate the role of LINC00847 and the LINC00847/miR-455-3p/HDAC4 mechanism in PC progression.Material and methods: The RNA levels of LINC00847, miR-455-3p and HDAC4 were determined by RT-qPCR. HDAC4 protein level was assessed by western blotting. Colony formation and CCK-8 assays were employed to test the proliferation of PC cells. Transwell and scratch assays were conducted to evaluate the cell invasive and migratory abilities, respectively. The effect of LINC00847 silencing on PC cells in vivo was verified using a mouse xenograft model. The correlation among LINC00847, miR-455-3p and HDAC4 was ascertained by dual-luciferase reporter (DLR) assay and Pearson's correlation analysis.Results: The result showed that LINC00847 mainly localized in the cytoplasm was upregulated in PC cells and tissues. Downregulating LINC00847 hindered migration, proliferation, and invasion of PC cells in vitro. Moreover, it also suppressed tumor growth in an in vivo xenograft model. LINC00847 was found to directly target miR-455-3p. miR-455-3p overexpression inhibited cell proliferation and invasion. In addition, HDAC4 was confirmed to be a target gene of miR-455-3p, and HDAC4 overexpression overturned the impact of LINC00847 knockdown on PC cell progression.Conclusions: Our findings reveal that LINC00847 potentially plays a key role in the carcinogenesis of PC progression. This effect may be mediated via regulating the miR-455-3p/HDAC4 axis. This study provides insights into the intricate molecular mechanisms underlying PC and opens avenues for potential therapeutic interventions.","PeriodicalId":8278,"journal":{"name":"Archives of Medical Science","volume":"20 3","pages":"847-862"},"PeriodicalIF":3.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of linezolid in the treatment of tuberculous meningitis: a meta-analysis. 利奈唑胺治疗结核性脑膜炎的疗效：一项荟萃分析。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-29 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/189905

Xiaoshu Liu, Daoyan Tang, Min Qi, Jian-Qing He

引用次数: 0

Licorice-induced severe hypokalemic rhabdomyolysis. 甘草诱发的严重低钾横纹肌溶解症。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-29 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/188909

Haihong Wang, Zhenhua Wen, Miao You

引用次数: 0

Circular RNA circ_001621 acts as a tumor promoter in lung cancer by regulating the miR-199a-3p/GREM1 axis. 环状 RNA circ_001621 通过调节 miR-199a-3p/GREM1 轴成为肺癌的肿瘤促进因子

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/174052

Jun Lei, Song Qiao

{"title":"Circular RNA circ_001621 acts as a tumor promoter in lung cancer by regulating the miR-199a-3p/GREM1 axis.","authors":"Jun Lei, Song Qiao","doi":"10.5114/aoms/174052","DOIUrl":"https://doi.org/10.5114/aoms/174052","url":null,"abstract":"Introduction: Investigating how circular RNAs (circRNAs) function during tumorigenesis may help uncover novel diagnostic markers for cancer treatment. The oncogenic role of circ_001621 has been verified in osteosarcoma, but its role in lung cancer has yet to be reported. This research is the first to investigate the circ_001621 expression and regulatory mechanism in lung cancer.Material and methods: RT-qPCR was performed to assess the circ_001621 expression levels in lung cancer cells and tissues. The influence of circ_001621 on the viability, invasive ability, and apoptosis of lung cancer cells was investigated through CCK-8, transwell, and caspase-3 activity experiments, respectively. A xenograft nude mouse model was designed to evaluate how circ_001621 functions in vivo. The RIP and luciferase reporter experiments confirmed the binding among circRNA, miRNA, and mRNA.Results: Circ_001621 was dramatically upregulated in lung cancer tissues and cells. Silencing circ_001621 in lung cancer cells reduced their viability and invasive ability but stimulated apoptosis. The nude mice experiment demonstrated that circ_001621 downregulation considerably stunted tumor growth in vivo. Additionally, circ_001621 could sponge miR-199a-3p. The inhibitor of miR-199a-3p improved the viability and invasion of cells while inhibiting apoptosis. Moreover, it offset the impact of circ_001621 on lung cancer cells. MiR-199a-3p was observed to target GREM1, and the downregulation of GREM1 could counteract miR-199a-3p-induced effects on lung cancer cells.Conclusions: The circ_001621/miR-199a-3p/GREM1 axis exhibits an association with the development of lung cancer, suggesting its potential as a future therapeutic target for the disease.","PeriodicalId":8278,"journal":{"name":"Archives of Medical Science","volume":"20 3","pages":"876-886"},"PeriodicalIF":3.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cinobufagin disrupts the stability of lipid rafts by inhibiting the expression of caveolin-1 to promote non-small cell lung cancer cell apoptosis. Cinobufagin 通过抑制 caveolin-1 的表达来破坏脂筏的稳定性，从而促进非小细胞肺癌细胞的凋亡。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/174578

Zhongqing Xu, Jinwei Li, Shuyu Fang, Mingzhu Lian, Changxiao Zhang, Jiahuan Lu, Kai Sheng

{"title":"Cinobufagin disrupts the stability of lipid rafts by inhibiting the expression of caveolin-1 to promote non-small cell lung cancer cell apoptosis.","authors":"Zhongqing Xu, Jinwei Li, Shuyu Fang, Mingzhu Lian, Changxiao Zhang, Jiahuan Lu, Kai Sheng","doi":"10.5114/aoms/174578","DOIUrl":"https://doi.org/10.5114/aoms/174578","url":null,"abstract":"Introduction: The study was designed to explore how cinobufagin (CB) regulates the development of non-small cell lung cancer (NSCLC) cells through lipid rafts.Material and methods: The effects of CB at gradient concentrations (0, 0.5, 1 and 2 µM) on NSCLC cell viability, apoptosis, reactive oxygen species (ROS) level, phosphorylation of Akt, and apoptosis- and lipid raft-related protein expression were assessed by MTT assay, flow cytometry and Western blot. Cholesterol and sphingomyelin were labeled with BODIPY to evaluate the effect of CB (2 µM) on them. Sucrose density gradient centrifugation was used to extract lipid rafts. The effect of CB on the expression and distribution of caveolin-1 was determined by immunofluorescence, quantitative reverse transcription polymerase chain reaction and Western blot. After overexpression of caveolin-1, the above experiments were performed again to observe whether the regulatory effect of CB was reversed.Results: CB inhibited NSCLC cell viability while promoting apoptosis and ROS level. CB redistributed the lipid content on the membrane surface and reduced the content of caveolin-1 in the cell membrane. In addition, CB repressed the activation of AKT. However, caveolin-1 overexpression reversed the effects of CB on apoptosis, AKT activation and lipid raft.Conclusions: CB regulates the activity of Akt in lipid rafts by inhibiting caveolin-1 expression to promote NSCLC cell apoptosis.","PeriodicalId":8278,"journal":{"name":"Archives of Medical Science","volume":"20 3","pages":"887-908"},"PeriodicalIF":3.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TREM-1 inhibition or ondansetron administration ameliorates NLRP3 inflammasome and pyroptosis in traumatic brain injury-induced acute lung injury. 抑制TREM-1或服用昂丹司琼可改善脑外伤诱导的急性肺损伤中的NLRP3炎性体和热蛋白沉积。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/174264

Fen Li, Na Qin, Yiqin Yu, Rui Dong, Xiaojie Li, Shenhai Gong, Zhenhua Zeng, Lin Huang, Hong Yang

{"title":"TREM-1 inhibition or ondansetron administration ameliorates NLRP3 inflammasome and pyroptosis in traumatic brain injury-induced acute lung injury.","authors":"Fen Li, Na Qin, Yiqin Yu, Rui Dong, Xiaojie Li, Shenhai Gong, Zhenhua Zeng, Lin Huang, Hong Yang","doi":"10.5114/aoms/174264","DOIUrl":"https://doi.org/10.5114/aoms/174264","url":null,"abstract":"Introduction: Recently, NLR family pyrin domain containing 3 (NLRP3) and pyroptosis have been reported to be involved in traumatic brain injury-induced acute lung injury (TBI-ALI). Studies have shown that triggering receptor expressed on myeloid cells-1 (TREM-1) may be one of the upstream molecules regulating NLRP3/pyroptosis, and 5-hydroxytryptamine type 3-receptor (5-HT3R) antagonists can inhibit NLRP3/pyroptosis. However, the role of TRME-1 in TBI-ALI, the therapeutic effect of 5-HT3R inhibition on TBI-ALI and its mechanism are still unclear. Therefore, this study aimed to evaluate the protective effect of ondansetron, a 5-HT3 inhibitor, on TBI-ALI, and to explore whether the underlying mechanism is related to the regulation of TREM-1.Material and methods: A TBI-ALI rat model was constructed via lateral fluid percussion (LFP) brain injury, and either TREM-1 inhibitor (LP17) or ondansetron was administered as needed.Results: TBI induced NLRP3 inflammasome, pyroptosis, and TREM-1 activation in rat lung tissues in a time-dependent manner. Inhibition of TREM-1 activity attenuated TBI-ALI; this is evident from reduced pathological scores, wet/dry ratios, and bronchoalveolar lavage fluid protein levels and alleviated NLRP3 inflammasome/pyroptosis. In addition, ondansetron reduced NLRP3 inflammasome/pyroptosis and alleviated TBI-ALI. Moreover, ondansetron reduced TREM-1 activation in macrophages and lung tissue.Conclusions: Ondansetron alleviated TBI-ALI. In terms of mechanism, TREM-1 promotes TBI-ALI via the NLRP3-related pyroptosis pathway, and the protective effect of ondansetron on TBI-ALI may be related to the inhibition of TREM-1.","PeriodicalId":8278,"journal":{"name":"Archives of Medical Science","volume":"20 3","pages":"984-996"},"PeriodicalIF":3.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence of adult-onset Still's disease and SAPHO syndrome. 成人型斯蒂尔病与 SAPHO 综合征并存。

IF 3 4区医学

Archives of Medical Science Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.5114/aoms/189502

Yunuo Wang, Haixu Jiang, Xinbo Yu, Qiuwei Peng, Zixiang Zheng, Yuanhao Wu, Chen Li

引用次数: 0