Aquatic Botany最新文献

{"title":"Assessing the response of Halimeda incrassata to temperature and light to predict its spread in the NE Atlantic","authors":"Belén Roger-Baynat,&nbsp;Marta Sansón,&nbsp;Daniel Álvarez-Canali,&nbsp;Carlos Sangil","doi":"10.1016/j.aquabot.2025.103968","DOIUrl":"10.1016/j.aquabot.2025.103968","url":null,"abstract":"<div><div>In recent decades, many species of tropical distribution have arrived in the Canary Islands due to tropicalization and direct and indirect anthropogenic activity. This is the case of the Caribbean seaweed <em>Halimeda incrassata</em> (Bryopsidales, Chlorophyta) that began to be detected in the eastern Atlantic in 2005 and is now found in the Canary Islands, Madeira, Azores, and the Mediterranean. In this study, an <em>ex-situ</em> experimental approach was carried out to assess the thermal and light tolerance of the species to understand its preferences and predict its expansion. Three experiments were performed, testing temperature and light individually and their interaction. The variables measured were thallus growth (percentage of surface area and increase of number of segments) and net photosynthesis. Results indicate that temperature is the most limiting factor to its growth, with higher temperatures being the most favorable (26–28ºC), while light intensity has a more negligible effect on the species. Consequently, <em>H. incrassata</em> could expand latitudinally until 40ºN, where temperature is over 20ºC for at least several months over the year. As an ecosystem engineer it can modify the environment and the communities where it is established, it is expected to compete with native species such as seagrasses, other macroalgae and garden eel. Further experiments, including acidification conditions and <em>in-situ</em> monitoring, should also be considered to complete the information on its growth limits.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"203 ","pages":"Article 103968"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Megaspore germination and sporophyte emergence requirements in the critically endangered endemic quillwort Isoëtes malinverniana","authors":"Abeli Thomas,&nbsp;Bacchetta Alice,&nbsp;Basilico Flavio,&nbsp;Berardo Pietro,&nbsp;Colli Alice,&nbsp;Colonna Ginevra,&nbsp;Pastor Bini Alessandra Balbina,&nbsp;Ruzzoli Alessia,&nbsp;Tucci Martina,&nbsp;Vesconi Giovanna Angela,&nbsp;Vitaloni Diana,&nbsp;Tarascio Martina","doi":"10.1016/j.aquabot.2025.103965","DOIUrl":"10.1016/j.aquabot.2025.103965","url":null,"abstract":"<div><div><em>Isoëtes malinverniana</em> is a critically endangered quillwort endemic to north-western Italy. Although some aspects of its reproductive biology are well known, megaspore and sporophyte germination ecology have never been studied in detail. In this study, we investigate and clarify the thermal requirements for megaspore germination and sporophyte emergence in <em>I. malinverniana</em>. We tested the effect of five constant temperatures (10, 15, 20, 25, 30 °C) and one alternating temperature (25/15 °C) on megaspore germination and sporophyte emergence in <em>I. malinverniana</em>. Moreover, we tested the effect of light and dark on megaspore germination and, the species self-compatibility and apomictic reproduction. Our results suggest that the optimal temperatures for megaspore germination and sporophyte emergence were 20 °C and 25/15 °C, reaching approximately 80 % germination at these temperatures after eight weeks. Spore germination did not occur at 10 °C and decreased at temperatures above 25 °C. Germination and emergence speed were also positively related to temperature. Megaspore and sporophyte of <em>I. malinverniana</em> germinated and emerged in the dark, but at a lower percentage than in full light. Our study suggests that <em>I. malinverniana</em> is self-compatible and cannot reproduce apomictically.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"203 ","pages":"Article 103965"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genetic variation of Najas marina and Najas major in the south-eastern Baltic region","authors":"L. Kamaitytė-Bukelskienė ,&nbsp;Z. Sinkevičienė ,&nbsp;D. Naugžemys ,&nbsp;J. Butkuvienė","doi":"10.1016/j.aquabot.2025.103971","DOIUrl":"10.1016/j.aquabot.2025.103971","url":null,"abstract":"<div><div>The present study examined the phenotypic and genetic differences between <em>Najas marina</em> L. s.s. and <em>Najas major</em> All. in the south-eastern Baltic region, testing reliable diagnostic markers. The key questions included species distribution patterns, the most distinctive morphological traits, the impact of ecological conditions on morphology, and the effectiveness of ISSR markers in evaluating genetic diversity and structure. The results were contrasted with those obtained from ITS and <em>rbc</em>L markers. We highlighted the potential and limitations of diagnostic tools used for by integrating morphological and molecular methods, and environmental data. Samples for the study were collected from diverse aquatic habitats in Lithuania, Latvia, and Poland, chosen based on herbarium data and literature. Morphological measurements and DNA extraction were performed using the freshly collected samples. Results show that morphological markers, specifically leaf widths, have significant diagnostic value for distinguishing <em>Najas marina</em> from <em>Najas major</em>. Therefore, assist in environmental management planning and ecological assessments as easy to use marker. Our results also show that ISSR markers produce distinct population structures confirming clear separation between <em>Najas marina</em> and <em>Najas major</em>, and underscoring the interspecific plasticity potentially related to specific environmental factors across sites. We confirmed that, currently, two species of the subgenus <em>Najas</em> coexist in separate waterbodies within the south-eastern Baltic region. We also argue that morphological markers are usually adequate for these species when considering morphological plasticity due to environmental properties. However, the use of barcoding markers or markers such as PCR ISSR are preferable when both species are growing sympatrically.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"203 ","pages":"Article 103971"},"PeriodicalIF":2.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obituary: Researcher and expert advisor Søren Wium-Andersen (1941–2025)","authors":"Kaj Sand-Jensen,&nbsp;Jens Borum","doi":"10.1016/j.aquabot.2025.103952","DOIUrl":"10.1016/j.aquabot.2025.103952","url":null,"abstract":"","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103952"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the photosynthetic ecophysiology of Myriophyllum aquaticum and congeneric milfoils and invasion potential into the Midwestern United States","authors":"Alyssa J. Anderson,&nbsp;Ryan M. Wersal,&nbsp;Christopher T. Ruhland","doi":"10.1016/j.aquabot.2025.103953","DOIUrl":"10.1016/j.aquabot.2025.103953","url":null,"abstract":"<div><div>Parrotfeather (<em>Myriophyllum aquaticum</em> (Vell.) Verdc.) is a heterophyllous, perennial aquatic invasive plant. Since parrotfeather’s arrival in the U.S. in the late 1800s, there is yet to be wide-spread establishment in the Midwestern United States (Midwest). The current study utilized pulse amplitude modulated chlorophyll fluorescence and gas exchange analyses to determine if there is a physiological mechanism that limits parrotfeather colonization in the Midwest. Fluorescence parameters were taken at water temperatures between 0 and 45°C, and gas exchange measurements were examined at 4, 25 and 35°C. The photosynthetic ecophysiology of parrotfeather was compared to Eurasian watermilfoil (<em>Myriophyllum spicatum</em> L.) and a hybrid strain of watermilfoil (<em>Myriophyllum spicatum</em> x <em>Myriophyllum sibiricum</em>) as the latter two plants are widespread throughout the Midwest. The ratio of variable to maximal fluorescence (F_v/F_m) in emergent and submerged parrotfeather averaged 0.80 and 0.65, respectively, at temperatures between 0 and 35°C and declined at &gt; 40°C. In addition, light-adapted quantum yield of photosystem II (ϕ_PSII) was lowest at temperatures between 0 and 10°C and 40–45°C in submersed leaves. Net photosynthetic rates were highest at 25°C for emergent and submerged parrotfeather and averaged 8.83 μmol m⁻² s⁻¹ and 0.00107 μmol g⁻¹ s⁻¹ FW, respectively. Carbon assimilation rates in submersed parrotfeather were significantly lower than those observed in the Eurasian and hybrid milfoils. Lastly, the light compensation points were consistently lower in submersed parrotfeather than the other milfoils with values ≤ 55 μmol m⁻² s⁻¹ at 4°C and 25°C. Based on current photosynthetic ecophysiology data, these species of <em>Myriophyllum</em> maintain positive photosynthetic rates at temperatures near 4°C.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103953"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of the red marine alga Gracilaria vermiculophylla grown under different light intensities","authors":"Takuya Kandori,&nbsp;Hiroyuki Mizuta,&nbsp;Toshiki Uji","doi":"10.1016/j.aquabot.2025.103949","DOIUrl":"10.1016/j.aquabot.2025.103949","url":null,"abstract":"<div><div>Light plays a vital role in seaweed growth by regulating photosynthesis and signaling metabolic processes. In natural habitats, seaweeds are exposed to fluctuating light conditions caused by environmental factors such as weather and tidal changes. Although the effects of light intensity on red algal growth have been studied physiologically, the underlying gene expression patterns remain poorly understood. Here, we examined the effect of light intensity on the growth of the economically important red alga <em>Gracilaria vermiculophylla</em>. Growth was markedly enhanced under high-light (HL) conditions compared with low-light (LL) conditions. To investigate the molecular basis of this enhanced growth, we performed transcriptome profiling using RNA-Seq. A total of 593 differentially expressed genes (DEGs) were identified between LL and HL. Among these, genes associated with nitrogen and carbon metabolism (e.g., nitrate reductase, ammonium transporter, and carbonic anhydrase) as well as β-glucanase, potentially involved in cell wall remodeling, were upregulated under HL. These findings provide new insights into the molecular mechanisms underlying light-enhanced growth in red macroalgae. The identified genes represent promising molecular targets for genetic engineering and may contribute to the development of strategies to enhance red algal productivity in aquaculture.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103949"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing seed germination and seedling establishment of the threatened seagrass, Zostera caespitosa: Implications for seed-based restoration","authors":"Dahyeok Im ,&nbsp;Seung Hyeon Kim ,&nbsp;Fei Zhang ,&nbsp;Zhaxi Suonan ,&nbsp;Hyegwang Kim ,&nbsp;Young Kyun Kim ,&nbsp;Hwi-June Song ,&nbsp;Min Ho Son ,&nbsp;Kun-Seop Lee","doi":"10.1016/j.aquabot.2025.103951","DOIUrl":"10.1016/j.aquabot.2025.103951","url":null,"abstract":"<div><div>The seagrass <em>Zostera caespitosa</em> is undergoing a notable decline within its native range along the Northeast Asian coast. We examined the effects of the seed source population, salinity (0, 15, and 30 psu), and temperature (5 and 20 °C) on germination rate and subsequent seedling growth under both field and controlled laboratory conditions to assess the potential for seed-based restoration. Seeds collected from Tongyeong (TY seeds) were larger than those from Jangmok Bay (JM seeds), resulting in an increased germination rate; however, seed size did not influence subsequent seedling growth. In the laboratory experiment, the optimal conditions for seed germination (15 psu and 5 °C) differed from those for seedling growth (15 psu and 20 °C), for both seed sources. In the field experiment, the seedling density from TY seeds was significantly higher than that from JM seeds during the first three months following germination, with no significant difference by the end of the experiment. These results suggest that additional environmental or biotic factors, such as competition and herbivory, strongly influenced seedling growth and successful establishment. Overall, our findings provide essential insights into the sexual reproduction of <em>Z. caespitosa</em> for seed-based restoration efforts and contribute to increasing public awareness of the conservation and management of this threatened species.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103951"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically verified mass material of Lemna in temperate East Europe: frequent L. minor – L. × japonica co-occurrence and new records of L. gibba and L. turionifera","authors":"Polina A. Volkova ,&nbsp;Maria O. Ivanova ,&nbsp;Lina G. Matieva ,&nbsp;Alexander A. Bobrov","doi":"10.1016/j.aquabot.2025.103950","DOIUrl":"10.1016/j.aquabot.2025.103950","url":null,"abstract":"<div><div>Native representatives of the economically important genus <em>Lemna</em> in Europe belong to the taxonomically problematic <em>L. minor</em> complex (in addition to well-distinguishable <em>L. trisulca</em>). Distribution of the taxa from this complex in East Europe and their diagnostic characters are still unclear. We explored 41 waterbodies in the model area of <em>ca.</em> 30 km² in Yaroslavl Region (Russia). We genetically verified (second intron length polymorphism in β-tubulin nuclear gene) taxonomic identity of 175 plants (2–10 plants per site). <em>Lemna minor</em> was the most frequent; either alone (20 sites), or together with <em>L. × japonica</em> (17 sites). In one site we found only <em>L. × japonica</em>. We have not found difference of the type, pH and mineralization of waterbodies with or without <em>L. × japonica</em> . Our quantitative data on mass live material of <em>L. minor</em> and <em>L. × japonica</em> did not support the existence of differences between them on the main diagnostic characters (color and shape of the fronds and sizes of papules on their upper side). We provided the first genetic documentation of <em>L. gibba</em> and <em>L. turionifera</em> occurrence in the region, extending their verified areas. We found flowering <em>Lemna</em> spp. with fully fertile pollen in 12 (29 %) waterbodies of all available types. For the first time we managed to verify genetically taxonomic affiliation of plants, flowering in nature (<em>L. minor</em> and <em>L. × japonica</em>). All genetically tested fruiting plants appeared to be <em>L. minor</em>.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103950"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of ABA and kinetin on the aquatic carnivorous plant Aldrovanda vesiculosa affects the morphology and phytohormone profiles in shoot apices","authors":"Lubomír Adamec ,&nbsp;Lenka Plačková ,&nbsp;Dardan Klos ,&nbsp;Karel Doležal","doi":"10.1016/j.aquabot.2025.103942","DOIUrl":"10.1016/j.aquabot.2025.103942","url":null,"abstract":"<div><div><em>Aldrovanda vesiculosa</em> is a rootless aquatic carnivorous plant forming turions (winter buds) as specific overwintering organs containing high abscisic acid (ABA) content. The high ABA content has the key role in regulating turion formation and maintaining turion dormancy. Here, we compared hormonal profiles (cytokinins, auxins, ABA) in shoot apices in <em>A. vesiculosa</em> grown in mini aquaria in a greenhouse at the end of the summer season after a 2-day priming with ABA (2 µM) and ABA together with an artificial cytokinin kinetin (KIN, 20 µM), to find out their effects and interactions on turion formation and morphology. During the next 11 days (without added hormones), the ABA application gently boosted the turion development but the rapid apical shoot growth was not influenced. The shoot apices were narrower and more condensed. The ABA+KIN application almost ceased the apical growth and retarded the turion development. Shoot apices were robust and wide. Turion development may thus interfere with apical shoot growth. The application of ABA alone did not significantly change the contents of cytokinins, auxins or ABA in shoot apices, but +ABA+KIN significantly decreased the total and active endogenous cytokinins and increased the ABA content threefold compared to the control. Thus, both added hormones affected the developing turions in a similar way which is known and expected in dormant organs of terrestrial plants. These results suggest that the coordination between ABA and cytokinins might be crucial for hormonal regulation of the onset of turion dormancy and turion development in aquatic plants.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103942"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative studies of invasive Elodea canadensis Michx. in two climatically different regions","authors":"Mariana Prokopuk ,&nbsp;Lesya Zub ,&nbsp;Maksym Netsvetov ,&nbsp;Silvia Martins ,&nbsp;Elizabete Marchante","doi":"10.1016/j.aquabot.2025.103944","DOIUrl":"10.1016/j.aquabot.2025.103944","url":null,"abstract":"<div><div><em>Elodea canadensis</em> is a widespread invasive alien aquatic species in Europe. However, there is growing evidence that increasing anthropogenic eutrophication and other factors are leading to a decline in the populations of this species. We compared the structure of plant communities and the phenotypic plasticity of <em>E. canadensis</em> individuals across communities in Ukraine and Portugal to assess whether the species responds similarly to environmental variation, and to identify differences in its distribution, community role, and the environmental factors influencing the formation of morphological traits of the species and biomass. In both countries, <em>E. canadensis</em> preferentially occurs in oligo-mesotrophic and meso-eutrophic waters, forming similar communities, but in Ukraine the communities in lentic water bodies were floristically more diverse. Populations with high biomass of <em>E. canadensis</em> in Ukraine were formed in lentic water bodies with good water exchange, as well as in lotic ecosystems of both Ukraine and Portugal. Environmental clustering revealed a distinct ecological separation among water bodies, with the strongest contrast observed between lower-nutrient, higher-elevation Ukrainian lakes and nutrient-rich, lowland mixed lotic systems in both countries. The morphological traits of <em>E. canadensis</em> differed significantly between these clusters, particularly shoot length and leaf width, highlighting phenotypic responses to environmental gradients. We assume that anthropogenic eutrophication affects <em>E. сanadensis</em> communities, and may lead to their decline or disappearance as trophic levels rise in both countries. Understanding these patterns is important for forecasting invasion dynamics under eutrophication and climate change, and for refining management strategies in freshwater ecosystems.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"202 ","pages":"Article 103944"},"PeriodicalIF":2.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}