Aquatic Botany最新文献

{"title":"Evaluating the combined methods of live planting and seed dispersal in restoration of Zostera marina (eelgrass) in Barnegat Bay, New Jersey by genetic diversity and pedigree analysis of restored populations","authors":"Nicole Rodriguez Ortiz,&nbsp;Nathan Cruz,&nbsp;Tiffany V. Santos,&nbsp;Paul A.X. Bologna ,&nbsp;James J. Campanella","doi":"10.1016/j.aquabot.2025.103917","DOIUrl":"10.1016/j.aquabot.2025.103917","url":null,"abstract":"<div><div><em>Zostera marina</em> (eelgrass) is a near-shore marine plant species that provides essential ecosystem services both as food and refuge for a variety of sea life, as well as a physical anchor for the littoral sediment along temperate coastlines. Due to disease, anthropogenic reductive effects, and recent natural disasters, Barnegat Bay, NJ has seen a reduction in natural populations of the species over the last few decades. Newly restored beds of <em>Z. marina</em> were planted in 2021 and 2022 by a combined method of live transplants and seed dispersal. In 2023, we acquired tissue samples from restored populations with the following objectives: A) test how successful the combined restoration method was at producing thriving populations, B) determine genetic diversity and “genetic health” as partial indicators of restoration success, and C) determine kinship in order to help correlate ancestry and efficacious seagrass growth progression. We found each restored population evinced increased genetic diversity over donor populations, based upon improved observed heterozygosity (mean 0.584), low inbreeding fixation levels (mean -0.146), and higher effective population sizes (mean 2.988). Principal Coordinate Analysis indicated that restored plants are primarily related to live planted donors, with alleles from seed donors in evidence. Cluster Analysis supports that restored individuals have genetic components of all donors, but also suggests environmental selection and genetic bottlenecking have occurred. Statistical analysis confirms the presence of bottlenecks in all restored populations. Overall, results support initial success in restored seagrass meadows. As global declines in seagrass beds continue, with the accompanying loss in genetic diversity, restoration techniques that demonstrate success in ecosystem service recovery and increased genetic diversity will guide practitioners in preservation and restoration of these habitats.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103917"},"PeriodicalIF":1.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Halophila stipulacea (Forsskål) Ascherson,1867 in the Dominican Republic, an invasive species that continues to expand across the Caribbean","authors":"Yira Rodríguez-Jerez ,&nbsp;Rita Sellares-Blasco ,&nbsp;Maria F. Villalpando ,&nbsp;Estefany Vargas-Pérez ,&nbsp;Andreina Valdez-Trinidad ,&nbsp;Someira Zambrano ,&nbsp;Yasmin D. Evangelista ,&nbsp;Sergio D. Guendulain-García ,&nbsp;Aldo Croquer","doi":"10.1016/j.aquabot.2025.103915","DOIUrl":"10.1016/j.aquabot.2025.103915","url":null,"abstract":"<div><div><em>Halophila stipulacea</em> (Forsskål) Ascherson,1867 is a seagrass species native to the Red Sea and the Persian-Indian Ocean, introduced 150 years ago into the Mediterranean Sea. <em>H. stipulacea</em> was first reported in the Caribbean in 2002; and in less than 15 years, it expanded across small island nations and Venezuela. Expected to continue expanding across its invasive range, monitoring programs aimed at spotting new geographic areas where <em>H. stipulacea</em> has successfully settled are vital. Here, we report for the first time an extensive meadow of <em>H. stipulacea</em> in Punta Salinas, Bani, Peravia Province, Dominican Republic (La Hispaniola). In November 2024, the species was spotted drifting inside Las Calderas Bay, and soon after, detected in a meadow located at the exposed side of Punta Salinas. After taxonomic confirmation, we conducted a survey during December 2024 to check for the presence of <em>H. stipulacea</em> meadows We observed a large <em>H. stipulacea</em> patch (1500 m) established on muddy sediments mixed with <em>Dictyota</em> spp., dead <em>Halimeda</em> spp. and other macroalgae. On average, the alien species covered 67.5 ± 36.7 %, whereas <em>Thalassia testudinum</em> covered &lt; 5 % of the substrate. While the impacts of <em>H. stipulacea</em> in La Hispaniola remain unknown, our results confirm that <em>H. Stipulacea</em> continues to expand, successfully invading coastal habitats across the Caribbean region.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103915"},"PeriodicalIF":1.9,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An aquatic pteridophyte, Salvinia, from the Subathu Formation (Late Paleocene–Early Eocene) of Himachal Himalaya, India, and its biogeographical implications","authors":"Ashif Ali ,&nbsp;Robert A. Spicer ,&nbsp;Tao Su ,&nbsp;Sampa Kundu ,&nbsp;Mahasin Ali Khan","doi":"10.1016/j.aquabot.2025.103916","DOIUrl":"10.1016/j.aquabot.2025.103916","url":null,"abstract":"<div><div><em>Salvinia</em>, a free-floating aquatic, rootless fern, has a rich fossil record in Cenozoic sediments of Southeast Asia, Europe, and North-South America. However, none have been reported from the Cenozoic sediments of India, until now. Here, we report the occurrence of vegetative remains (both impressions and compressions), along with trichome bases, resembling both morphologically and anatomically those of the extant genus <em>Salvinia</em> Séguier from the Late Paleocene-Early Eocene of the Subathu Formation in the Mandi District of Himachal Pradesh, northwestern India. These newly discovered fossils are characterized by round, elliptical, or elongate fronds, with small hexagonal or quadrangular areolaes, usually 8–16 tubercles in a row between adjacent lateral veins, and craspedodromous lateral veins lacking marginal loops. Anatomically, epidermal cells are quadrangular or polygonal with diacytic stomata composed of two guard cells and numerous conical, filiform, slightly enlarged, and curved non-glandular trichomes. We describe these Eocene specimens as a new species, namely <em>Salvinia indica</em> A. Ali &amp; M. A. Khan sp. nov. The discovery of this species suggests that the Eocene Subathu Formation represents sediments deposited in mostly calm freshwater bodies such as very low-energy meandering rivers, streams, or wetland ponds.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103916"},"PeriodicalIF":1.9,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhodolith beds in the Eastern Tropical Pacific: Habitat structure and associated biodiversity","authors":"Celeste A. Diaz-Licona ,&nbsp;Nadine Schubert ,&nbsp;Vladimir González-Gamboa ,&nbsp;Fernando Tuya ,&nbsp;Juan Carlos Azofeifa-Solano ,&nbsp;Cindy Fernández-García","doi":"10.1016/j.aquabot.2025.103914","DOIUrl":"10.1016/j.aquabot.2025.103914","url":null,"abstract":"<div><div>Rhodolith beds (RBs) are globally distributed marine benthic habitats and recognized biodiversity hotspots of conservation interest. However, considerable regions of the world’s oceans remain unknown in terms of the presence, distribution, structure, and associated biodiversity of such habitats. In the Eastern Tropical Pacific (ETP), even basic information about these habitats is still extremely scarce. To fill this gap, we characterized the habitat structure and associated biodiversity of four shallow-water RBs at Cocos Island, Costa Rica. Specifically, information regarding rhodolith structural attributes (size, morphology, and CaCO₃ content), as well as habitat characteristics (rhodolith nodule density, biomass and CaCO₃ standing stocks) were collected. Moreover, the diversity and abundance of associated organisms, including macroalgae, invertebrates and fishes, were determined. Our study shows that Cocos Island harbors dense RBs (1100 to &gt;4500 nodules m⁻²) with substantial carbonate stocks (14–22 kg m⁻²), which provide habitats for a wide array of species (158 recorded species). This study adds 58 new records of RB-associated species to previously published records for the island, which increases the total number of species to 316, including 51 species endemic to the ETP and four species categorized as vulnerable by the IUCN. Our results also indicate that community composition and abundance of organisms vary among RBs, likely due to differences in rhodolith morphologies and sizes and/or local environmental conditions. Further research efforts are warranted to identify the drivers for these differences, as well as expanding studies towards other RBs at Cocos Island and in the ETP in general.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103914"},"PeriodicalIF":1.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144211830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of desiccation time on performance of a cultured (Neopyropia yezonensis) and fouling alga (Ulva prolifera)","authors":"Yifei Shen ,&nbsp;Ruyan He ,&nbsp;Yuqing Sun ,&nbsp;Shuang Li ,&nbsp;Yinqing Zeng ,&nbsp;Peimin He ,&nbsp;Jianheng Zhang","doi":"10.1016/j.aquabot.2025.103912","DOIUrl":"10.1016/j.aquabot.2025.103912","url":null,"abstract":"<div><div><em>Neopyropia yezoensis,</em> a kind of red alga (Rhodophyta) that is a source of nori, is a highly valuable macroalga widely cultivated in coastal regions of East Asia. However, in recent years, <em>Neopyropia</em> cultivation areas have been frequently disturbed by green tide algae, particularly <em>Ulva prolifera</em>. To address this issue, we conducted a seven-day experiment subjecting both <em>N. yezoensis</em> and raft-attached <em>U. prolifera</em> to desiccation treatment twice daily, with exposure times ranging from 0 to 6 hours per treatment. The physiological responses were evaluated through microscopic structure analysis, maximum quantum yield of photosystem II (Fv/Fm), biomass measurements, and pigment content determination. Results showed that increasing desiccation time significantly affected the physiological status of <em>U. prolifera</em>. With prolonged desiccation time, both <em>U. prolifera</em> and <em>N. yezoensis</em> exhibited decreased Fv/Fm values, reduced biomass growth rate, and significant changes in pigment content. Desiccation periods of 5–6 hours reduced the photosynthetic efficiency of photosystem II (PSII) in <em>U. prolifera</em>. Although a single desiccation treatment temporarily inhibited the physiological status of <em>N. yezoensis</em>, after undergoing desiccation–rehydration cycles, the alga not only recovered its physiological function but also exhibited higher Fv/Fm values than the continuously submerged control group. However, for treatments with desiccation times exceeding 5 hours, productivity was somewhat affected, with the growth rate being approximately 24 % lower than that of the continuously submerged group. This indicates that desiccation-rehydration cycles promote the physiological status and biomass of <em>N. yezoensis</em>, demonstrating its superior adaptation to desiccation conditions. This study identified 5 hours as the optimal desiccation duration for <em>N. yezoensis</em> cultivation. Under these conditions, <em>N. yezoensis</em> maintained high growth rates and favorable physiological status while effectively inhibiting <em>U. prolifera</em> growth, providing a viable method for controlling <em>U. prolifera</em> in cultivation areas.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103912"},"PeriodicalIF":1.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seagrass fossils from the lower Miocene Morozaki Group in Aichi Prefecture, central Japan","authors":"Toshihiro Yamada","doi":"10.1016/j.aquabot.2025.103913","DOIUrl":"10.1016/j.aquabot.2025.103913","url":null,"abstract":"<div><div>Two new seagrass fossils are reported from the upper lower Miocene Morozaki Group (18.0–17.6 Ma) of central Japan: <em>Thalassites morozakiensis</em> T. Yamada, sp. nov., and <em>Maresurculus aichiensis</em> T. Yamada, gen. et sp. nov. The former bears strap-shaped, eligulate leaves enclosed within a fibrous sheath, suggesting an affinity with the extant subfamily Hydrilloideae (Hydrocharitaceae). The latter is characterized by ligulate leaves shed without leaving a sheath on the stem, indicating a relationship to extant genera of Cymodoceaceae, such as <em>Amphibolis</em> and <em>Thalassodendron</em>. These fossils represent the first reliable records of seagrasses from the early Miocene, consistent with divergence-time estimates for marine Hydrilloideae and Cymodoceaceae based on molecular clocks. Additionally, they imply that seagrasses extended their range to higher latitudes than today during the late early Miocene, a period marked by global warming.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103913"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demographic and morphological responses of intertidal eelgrass (Zostera marina) transplanted across sediment conditions","authors":"Jennifer L. Ruesink ,&nbsp;Fiona C. Boardman ,&nbsp;Bryan A. Briones Ortiz ,&nbsp;Christopher R. Jendrey ,&nbsp;Kerry Ann Naish","doi":"10.1016/j.aquabot.2025.103904","DOIUrl":"10.1016/j.aquabot.2025.103904","url":null,"abstract":"<div><div>Eelgrass (<em>Zostera marina</em>) shows substantial spatiotemporal variation in morphological and demographic traits that can arise from phenotypic plasticity. These traits commonly shift across light and depth gradients, as well as seasonally, but less consistency has been demonstrated across sediment conditions. We conducted two field transplant experiments in which seedlings or overwintering shoots of <em>Z. marina</em> were moved among sand and muddy-sand sites within a bay (Willapa Bay, Washington, USA). Shoots were regularly re-marked and measured for size, asexual, and sexual reproduction for 1–1.5 year, and upon collection, for rhizome extension and root investment. Seedlings, which were sourced from one site, had poor survival in muddy-sand, but smaller size and more branching with less flowering in sand. Shape also exhibited phenotypic plasticity, with relatively wide leaves and longer rhizome internodes at the sandier site. Overwintering shoots, which were transplanted among four sites in a fully reciprocal design, had lower survival and size after 6 months at sand compared to muddy-sand sites, but with greater root investment at collection. Shoots from one muddy-sand site, regardless of outplant site, maintained relatively short internodes over 1.5 years. Overall, shoots tended to remodel in summer as a function of sediment type, while internode extension was phenotypically plastic in both experiments, as well as fixed by source. Because sediment correlated with water movement, eelgrass traits expressed in sand could reduce drag (smaller above-ground size) and provide anchorage (root investment). Shorter internodes at muddy-sand outplant sites and from one muddy-sand source site could influence space-filling and foraging strategies. Across experiments, poor survival of seedlings relative to vegetative shoots in muddy-sand may reflect low-oxygen conditions that are challenging for early life stages of eelgrass to overcome. The substantial trait variation in transplants across sediment type could be a response to below-ground chemistry and above-ground hydrodynamics, but these drivers were not distinguishable with the study design.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103904"},"PeriodicalIF":1.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144204534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary assessment of a nutrient pollution indicator for application to tropical seagrasses of Puerto Rico","authors":"James E. Kaldy ,&nbsp;Cayla Sullivan ,&nbsp;Angel Dieppa ,&nbsp;Evelyn Huertas ,&nbsp;Mark Reiss ,&nbsp;Izabela Wojtenko ,&nbsp;Julia Perzley ,&nbsp;Ivelisse Cappielo Cosme ,&nbsp;Marco Orizondo Lugo","doi":"10.1016/j.aquabot.2025.103903","DOIUrl":"10.1016/j.aquabot.2025.103903","url":null,"abstract":"<div><div>Seagrass health is often used as an indicator of water quality and plant tissue nitrogen content has long been used as an indicator of nitrogen availability, but it is not a sensitive early indicator of nutrient over enrichment. A critical characteristic for an early indicator is that it can detect increased nutrient availability before seagrasses die. The seagrass nutrient pollution indicator (NPI) integrates leaf tissue nitrogen content and leaf areal mass to track nutrient loading. We assess the underlying assumptions associated with the NPI metric and explore the application of the metric to three tropical seagrasses <em>Thalassia testudinum</em>, <em>Halodule wrightii</em>, and <em>Halophila stipulacea</em>. In June 2022 we sampled ten seagrass sites across three embayments in Puerto Rico. We used water column and sediment nutrient measurements, seagrass stable isotope analyses (%N and δ¹⁵N) and we made morphological measurements (leaf areal mass, mg dry weight cm ⁻²) to evaluate the NPI relative to anticipated environmental gradients. <em>T. testudinum</em> sheath material, <em>H. wrightii</em> and <em>H. stipulacea</em> met the assumptions of the NPI method, suggesting that the NPI metric may be applied to these tropical species. Jobos Bay sites had isotopically light δ¹⁵N values suggesting that either local N fixation or import of allochthonous N fixation are dominant sources. In Guánica Bay, heavy δ¹⁵N values suggest either wastewater inputs or internal recycling of N are the dominant source. Additional research is required, but the evidence suggests that with further development and validation the NPI metric with δ¹⁵N could be appropriate for tropical seagrass species.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"201 ","pages":"Article 103903"},"PeriodicalIF":1.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastome evolution and phylogenomic insights into the evolution of the African submerged endemic genus Lagarosiphon (Hydrocharitaceae)","authors":"Zhi-Zhong Li ,&nbsp;Ying Wang ,&nbsp;Andrey Efremov","doi":"10.1016/j.aquabot.2025.103901","DOIUrl":"10.1016/j.aquabot.2025.103901","url":null,"abstract":"<div><div>The genus <em>Lagarosiphon</em>, a remarkable group of fully submerged plants within the family Hydrocharitaceae, is native to tropical Africa. However, the phylogenetic relationships among its species have long remained elusive due to a lack of molecular data. In this study, we assembled and compared the plastomes of three <em>Lagarosiphon</em> species from Africa. By integrating additional data from public databases, we reconstructed the phylogeny and evolutionary history of the genus. Our results revealed that the plastomes of <em>Lagarosiphon</em> were conserved but exhibited notable length variation, likely due to the accumulation of dispersed repeats. Furthermore, we found that nucleotide diversity in the intergenic spacer regions was significantly higher than in the protein-coding genes. Such regions as <em>ycf1</em>, <em>infA</em>, <em>rpl23</em>-<em>trnI-CAU</em>, and <em>ycf1</em>-<em>ndhF</em> were recognized as mutation hotspots, highlighting their potential as important markers for future population genetics and phylogenetic studies. Phylogenomic analysis strongly supported <em>Lagarosiphon</em> as a monophyly, with the most recent common ancestor estimated to have occurred around 29.95 Ma. The diversification of this genus in Africa is likely a response to rapid climatic and geological changes during the Miocene. Our study provides valuable molecular resources for the taxonomic and phylogenomic investigation of the genus <em>Lagarosiphon</em>.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"200 ","pages":"Article 103901"},"PeriodicalIF":1.9,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of plant growth-promoting rhizobacteria associated with Posidonia oceanica meadow in the North coast of Monastir, Tunisia","authors":"Khaled Elmnasri ,&nbsp;Mohamed El Gtari","doi":"10.1016/j.aquabot.2025.103902","DOIUrl":"10.1016/j.aquabot.2025.103902","url":null,"abstract":"<div><div>Given the significant ecological and socio-economic roles played by seagrass beds in the Mediterranean Sea, there is an urgent need to explore effective approaches for the conservation and restoration of <em>Posidonia oceanica</em> meadows. The characterization and utilization of plant growth-promoting rhizobacteria (PGPRs) appear to be one of the most innovative methods. In this study, metataxonomic analysis revealed the dominance of bacteria belonging to <em>Acinetobacter</em> (36.7 %), <em>Halodesulfovibrio</em> (38 %), <em>Vibrio</em> (9 %), <em>Lactobacillus</em> (1.3 %), <em>Arcobacter</em> (3 %), and <em>Desulforhopalus</em> (1 %) in the rhizosphere of <em>P. oceanica</em>, sampled from the northern coast of Monastir. Compared to the rhizosphere samples, a loss of biodiversity was observed in bulk sediment from adjacent erosive intermattes, with the disappearance of <em>Halodesulfovibrio</em>, <em>Anaerovorax</em>, <em>Anaerotignum</em>, and <em>Marinomonas</em> representatives, alongside a reduction in the abundance of <em>Vibrio</em>, <em>Arcobacter</em>, and <em>Desulforhopalus</em>. Isolation on Marine Agar medium allowed the purification of 42 bacterial strains, assigned to the genera <em>Vibrio</em>, <em>Pseudoalteromonas, Alteromonas, Bermanella, Marinomonas</em>, and <em>Tritonibacter</em>, with the detection of a new species closely related to <em>V. viridaestus</em>. The ability of these bacteria to produce hydrogen cyanide (HCN), ammonia (NH3), indole-3-acetic acid (IAA), and enzymes such as esterase, amylase, protease, agarase, and cellulase was also evaluated. The most promising strains for growth promotion were identified as <em>Pseudoalteromonas shioyasakiensis</em> PRL9 and PRL10, <em>Alteromonas abrolhosensis</em> PRA3, and <em>Marinomonas aquiplantarum</em> PR10. In vivo trials of the PGPR consortium on <em>Posidonia</em> seedling growth are planned.</div></div>","PeriodicalId":8273,"journal":{"name":"Aquatic Botany","volume":"200 ","pages":"Article 103902"},"PeriodicalIF":1.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}