Genetic diversity and population structure of four Trapa species from the Yangtze River and Amur River basins, China

IF 1.9 4区生物学 Q2 MARINE & FRESHWATER BIOLOGY

Aquatic Botany Pub Date : 2025-04-02 DOI:10.1016/j.aquabot.2025.103887

Miriam Chepkwemoi Tengwer , Godfrey Kinyori Wagutu , Henry Kariuki Njeri , Wei Li , Yuan-Yuan Chen , Xiang-Rong Fan

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引用次数: 0

Abstract

Water chestnuts (Trapa L.) are annual floating-leaved macrophytes with significant economic and ecological values. Genetic information assessment of wild germplasm resources is essential to the effective utilization and cultivation breeding of this genus. Here, Expressed Sequence Tag-Simple sequence repeat (EST-SSR) markers were developed and used to evaluate the genetic diversity and population structure of the four common wild Trapa species from the two diversity centers of Trapa in China: the Yangtze River Basin (YRB) and the Amur River Basin (ARB). For the 12,178 EST-SSRs, the dinucleotide (55.3 %) was the predominant type. Using 19 primers, higher levels of genetic variation were detected in the two Trapa species from YRB (T. bispinosa: He= 0.530; T. quadrispinosa: He=0.498) than that of the species from ARB (T. manshurica: He = 0.279; T. maximowiczii: He=0.415). This might be attributed to the smaller population size and lower hydrological connectivity among populations for the two species of ARB. Correspondingly, the population differentiation levels within the two Trapa species in ARB (T. manshurica: F_ST=0.383; T. maximowiczii: F_ST=0.239) were higher than that of the species in YRB (T. bispinosa: F_ST=0.169; T. quadrispinosa: F_ST=0.124). The clustering analyses (UPGMA, PCoA and SRUCTURE) consistently showed that all the individuals were divided into three branches: the accessions from T. maximowiczii and T. manshurica were clustered based on their species origins; the same genetic group was shared by the individuals from T. bispinosa and T. quadrispinosa, which possessed similar fruit morphological characteristics in addition to the number of waist horns. Findings of this study suggested the Trapa populations with high genetic diversity should be prioritized for conservation in YRB, while each population should be equally protected because of obvious genetic differentiation among populations in ARB.

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中国长江流域和阿穆尔河流域 4 个 Trapa 种的遗传多样性和种群结构

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来源期刊

Aquatic Botany 生物-海洋与淡水生物学

CiteScore

3.80

自引率

5.60%

发文量

审稿时长

6 months

期刊介绍： Aquatic Botany offers a platform for papers relevant to a broad international readership on fundamental and applied aspects of marine and freshwater macroscopic plants in a context of ecology or environmental biology. This includes molecular, biochemical and physiological aspects of macroscopic aquatic plants as well as the classification, structure, function, dynamics and ecological interactions in plant-dominated aquatic communities and ecosystems. It is an outlet for papers dealing with research on the consequences of disturbance and stressors (e.g. environmental fluctuations and climate change, pollution, grazing and pathogens), use and management of aquatic plants (plant production and decomposition, commercial harvest, plant control) and the conservation of aquatic plant communities (breeding, transplantation and restoration). Specialized publications on certain rare taxa or papers on aquatic macroscopic plants from under-represented regions in the world can also find their place, subject to editor evaluation. Studies on fungi or microalgae will remain outside the scope of Aquatic Botany.