Annals of Medical Research最新文献

{"title":"Time-dependent exposure to venetoclax induces ferroptosis in human neuroblastoma cells via upregulated transferrin gene expression and lipid peroxidation","authors":"Zübeyir Elmazoğlu, Erva Özkan","doi":"10.5455/annalsmedres.2025.07.192","DOIUrl":"https://doi.org/10.5455/annalsmedres.2025.07.192","url":null,"abstract":"Aim: Neuroblastoma is among the most widely diagnosed extracranial tumors in pediatric patients with poor survival rates. Despite the available treatment options, alternative treatment strategies are required, especially for the high-risk patients. Venetoclax (VTX) is a small molecule inhibitor of anti-apoptotic protein Bcl-2, originally approved for acute myeloid and chronic lymphocytic leukemia. Mounting evidence suggests that VTX may be a promising agent against other type of cancers as well. However, there are limited data on the alternative mechanisms of VTX toxicity. The aim of the present study was to unveil the true potential of the agent against neuroblastoma. In this regard, the effect of VTX on iron metabolism and ferroptotic cell death in neuroblastoma cells was investigated for the first time. Materials and Methods: Cell viability was determined with MTT assay. Oxidative stress, intracellular iron content and lysosomal integrity were visualized with confocal microscopy. MDA assay was performed to detect lipid peroxidation. The gene expressions of TFR, FPN1 and GPX4 were determined by RT-qPCR. Results: Results showed that VTX reduces cell viability significantly in both time and concentration-dependent manner, which was reversed by the addition of ferroptosis inhibitors. Further experiments revealed that VTX induces ROS generation, leads to lysosomal degradation and iron accumulation followed by lipid peroxidation, all of which are ferroptosis markers. RT-qPCR analyses indicated that VTX upregulates TFR gene expression while downregulating GPX4 and FPN1. Conclusion: In conclusion, the present study demonstrated for the first time that VTX activates ferroptotic pathways in neuroblastoma cells and may be considered a promising agent for add-on therapies.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"32 12","pages":"552-552"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shikimic Acid, Cyclohexene as a Hydroaromatic Intermediate, Harbors Potent in vitro Antimicrobial Activity","authors":"Tuba Ünver","doi":"10.5455/annalsmedres.2024.04.075","DOIUrl":"https://doi.org/10.5455/annalsmedres.2024.04.075","url":null,"abstract":"Aim: Shikimic acid is a hydroaromatic intermediate in the amino acid biosynthesis pathway in bacteria, fungi, and plants. Shikimic acid is an essential agent in applied sciences, especially in pharmacy and medicine. It can be used as a reactant in organic synthesis to obtain various medicinal drugs. This study aims to evaluate the antibacterial and antifungal properties of shikimic acid comparatively and to determine the minimum inhibitory concentrations (MIC) of shikimic acid against the tested Candida and bacterial species. Materials and Methods: The inhibitory effect of pure (98.85%) shikimic acid was tested on five bacteria (two Gram-positive and three Gram-negative) and five Candida species. The broth two-fold microdilution method was used to determine the MIC values against the tested microorganisms, and the viability of microorganisms treated with shikimic acid was determined using resazurin sodium salt. Results: The MIC values of shikimic acid against Candida glabrata, Candida albicans, Candida krusei, Candida tropicalis and Candida parapsilosis were determined to be between 250 and 31.25 mg/mL. However, the MIC values against bacterial species, including Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Enterobacter aerogenes, ranged between 15.625 and 3.906 mg/mL. Therefore, the antibacterial effect of shikimic acid is almost twenty times stronger than its antifungal effect. Conclusion: This preliminary study shows that shikimic acid is also usable as an antibacterial and antifungal as a potential therapeutic agent in addition to its proven antiviral properties. Further, advanced studies in this sense will be eye-opening.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"31 7","pages":"504-504"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of pneumothorax, pneumomediastinum, and other factors on the mortality and morbidity in patients with viral infections","authors":"l Murat, mit Karatepe","doi":"10.5455/annalsmedres.2024.01.016","DOIUrl":"https://doi.org/10.5455/annalsmedres.2024.01.016","url":null,"abstract":"Aims: This study compares the impact of pneumothorax and pneumomediastinum on the mortality and morbidity of respiratory viral infection patients using blood parameters and the Systemic Immune Inflammation Index (SII) among those who developed these complications versus those who did not. This study examines the effects of pneumothorax and pneumomediastinum on the mortality and morbidity of patients with respiratory virus infections. It analyses blood parameters and the Systemic Immune Inflammation Index (SII) in patients who acquired these complications compared to those who did not. Methods: A retrospective evaluation of 2246 COVID-19 patients treated in our medical facility from August 2020 to February 2021 was performed. The investigation comprised 118 patients hospitalized owing to the respiratory viral infection in this cohort. Patients were divided into two groups: those who had pneumothorax and/or pneumomediastinum and those who did not develop these problems, with equal representation in each. The study examined criteria such as metabolic profiles, hemogram results, length of hospital stay, SII levels, and the incidence of pneumothorax, with an emphasis on their relationship to morbidity and mortality outcomes. We conducted a retrospective assessment of 2246 COVID-19 patients who received treatment at our medical facility between August 2020 and February 2021. This study included a total of 118 patients who were admitted to the hospital due to a respiratory virus illness. The patients were categorised into two groups: one consisting of individuals who experienced pneumothorax and/or pneumomediastinum, and the other consisting of individuals who did not acquire these complications. Both groups had an equal number of participants. The study analysed many factors including metabolic profiles, hemogram results, length of hospital stay, SII levels, and the incidence of pneumothorax, with a focus on their association with morbidity and mortality outcomes. Results: Notably, female patients who developed pneumothorax while receiving COVID-19 therapy had a higher fatality rate. Patients that had pneumothorax also had raised leukocyte and neutrophil counts, as well as increased SII and lower total protein and albumin levels. These data underscore the potential significance of particular blood measures, along with the occurrence of pneumothorax, as indicators for mortality in patients with respiratory viral infections. Significantly, female patients who experienced pneumothorax during COVID-19 treatment exhibited a worse mortality rate. Patients with pneumothorax had elevated leukocyte and neutrophil counts, along with heightened systemic immune-inflammation index (SII) and decreased levels of total protein and albumin. The data highlight the potential importance of specific blood measurements, as well as the presence of pneumothorax, as indications for death in patients with respiratory virus infections. Conclusions: To improve patient outcomes, f","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"31 5","pages":"365-365"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Electrocardiogram and Inflammation Parameters in Patients with First Episode Mania","authors":"Yasar c, Sabri Abu, S. Ayhan, Bulut c, A. Tekin","doi":"10.5455/annalsmedres.2022.12.377","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.12.377","url":null,"abstract":"Aim: This study examined inflammation markers and electrocardiogram data in patients with first-episode mania (FEM). It aimed to examine the inflammation processes in the early stages of the disease and the frontal QRS-T (fQRS-T) angle in bipolar disorder (BD). Materials and Methods: After exclusion criteria, 53 FEM patients and 59 healthy controls (HC) were included in the study. Sociodemographic, electrocardiogram (ECG) and lab data of the participants were evaluated. Results: Mean of age and gender distribution did not differ between the groups (p=.743, p=.294, respectively). The heart rate of the FEM group was significantly higher than the HC group (p<.001). fQRS-T was significantly wider in the FEM group than in healthy controls (p=.20). Albumin was significantly lower in the FEM group than in the HC group (p<.001). CRP and CAR values were significantly higher in the FEM group than in HC (p=.009 and p=.011, respectively). In the FEM group, HDL value was significantly lower than HC, while LDL value was significantly higher than HC (p=<.001 and p=.001, accordingly). MHR and AIP values were significantly greater in the FEM group than in HC (p=<.001 and p=.006, respectively). The fQRS-T angle in FEM patients was associated with MHR (r=.37). Conclusion: This study is unique in evaluating inflammatory markers, fQRS-T angle, and heart rate in FEM patients. In this study, we found significantly higher inflammatory markers of MHR, CRP, and CAR in FEM patients in the control group. We found that these findings were consistent with the literature. Since the fQRS-T angle was wider in the FEM group, the cardiovascular risk may be increased in the early stages of BD disease.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74425735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The correlation between vitamin D levels and inflammation, as well as the Prognostic Nutritional Index (PNI), in people who are over the age of 60.","authors":"Nurgül Arslan, B. Yaprak","doi":"10.5455/annalsmedres.2022.12.396","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.12.396","url":null,"abstract":"Aim: Elderly populations often have insufficient vitamin D (vit-D), which may contribute to autoimmune diseases and inflammation. Few studies have examined vit-D levels and immune function in humans. The study looked at vit-D levels and inflammatory markers in older adults. Material and Methods: Retrospective research was done. The hospital's records were searched for the study's data. Participants were 65-year-olds who applied to the internal medicine outpatient clinic between May 2020 and May 2022. Each person's vit-D, CRP, Lymphocyte/Monocyte, Neutrophil/Lymphocyte, Platelet/Lymphocyte, Ferritin, and Prognostic nutritional index (PNI) levels were measured. Results: The information from 2118 patients was analyzed as part of the study. Of those patients, 324 met the requirements for participation in the research, and the participants' average age was 73.21 years, with a standard deviation of 5.24 years. The average level of vit-D found in people was 14.18 nmol/L, with a standard deviation of 6.14 nmol/L. It was discovered that there was a negative correlation that was statistically significant between the individuals' levels of vit-D and the ratio of CRP to MPV (p less than 0.05). Vit-D and PNI were found to have a correlation of r=0.205, which was found to be positive and significant (p <0.05). Every 0.721-unit increase in PNI will result in an additional 1 unit of 25(OH) vit-D. It was found that the CRP (mg/L) variable contributed significantly to the model, and it was discovered that the presence of 1 unit of 25(OH) vit-D was increased for every 0.202 unit decrease (p <0.05). Conclusion: Insufficient vitamin 25(OH)D is found in the elderly population. 25(OH) vit-D has a negative relationship with CRP (mg/L) and MPV (fL), but a positive relationship with PNI, an indicator of nutritional status. The study suggests that if an elderly person is disabled, nutritional supplements should be provided.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"2005 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72617783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of the Serum Albumin Levels at the Time of Admission on the Duration of Hospitalization in Indirect Hyperbilirubinemia","authors":"Seda Aydoğan, N. Fettah, H. Akduman, C. Geyik, A. lu","doi":"10.5455/annalsmedres.2022.12.366","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.12.366","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77644869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of pain during endovenous laser ablation of the great saphenous vein with ultrasound-guided femoral nerve block with local anesthesia","authors":"O. Hanbeyoğlu, S. Aydin","doi":"10.5455/annalsmedres.2022.11.338","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.11.338","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85947421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fetal Hemoglobin Altering Effects of KLF1, BCL11A rs11886868 and XmnI-HBG2 on Transfusion Dependent Beta Thalassemia Patients: Preeliminary Study","authors":"Y. Arıkan, B. Yolcular, Erdal Lu, I. Keser","doi":"10.5455/annalsmedres.2023.02.059","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.02.059","url":null,"abstract":"Aim: High fetal hemoglobin value is one of the quantitative trait in beta thalassemia and may effect transfusion dependency status of beta thalassemic cases. There are population-based differences about known genetic modifiers of different fetal hemoglobin values. We aimed to find if high fetal hemoglobin value are caused by XmnI-HBG2 polymorphism, rs11886868 of BCL11A or KLF1 whole gene mutations. Material and Methods: Genotyping procedure of thirty well re-defined and characterized transfusion dependent beta thalassemia patients was conducted via either sanger sequencing or and PCR-RFLP. Statistic analysis of groups and multiple logistic regression analysis of related genotypes were performed. Results: We found strong correlations between transfusion dependency and fetal hemoglobin levels (P<0.05). IVS.I.110 (G>A) homozygous mutation was found to be predominant in HBB gene. Lower fetal hemoglobin levels were seen in IVS.I.110 (G>A) homozygous group (P<0.05). Total count of variations among the three modifier genes BCL11A polymorphism was leading first. We did not observe any statistically significant relationship in patients with beta thalassemia major patients who have high fetal hemoglobin values between three modifiers group (P>0.05). Conclusion: This is the first research report from Turkey in terms of 3 different modifiers were analysed and evaluated. Since some cases have more than one variations in these three modifiers, involving higher sample size may overcome this challenge. Other genomic alterations rather than XmnI-HBG2, variations of BCL11A rs11886868 and mutation profile of KLF1 gene, which could decrease or abolish the effect of gamma globin repressors, may have more direct role with high fetal hemoglobin levels in patients with transfusion dependent beta thalassemia in Turkey.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76777543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of CO-RADS, RT-PCR and Chest CT Score; A report of 519 cases","authors":"Aylin Kus, Y. Okuturlar, C. Kalaycı, Yasemin Gundogdu, ftihar Koksal, E. Kızılkaya","doi":"10.5455/annalsmedres.2022.08.243","DOIUrl":"https://doi.org/10.5455/annalsmedres.2022.08.243","url":null,"abstract":"<jats:p />","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85780139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between spousal support and depression, anxiety, stress, and prenatal attachment in high-risk pregnancies","authors":"A. M. Res, B. Çallı, H. Dikmen","doi":"10.5455/annalsmedres.2023.03.070","DOIUrl":"https://doi.org/10.5455/annalsmedres.2023.03.070","url":null,"abstract":"Objective: To examine the relationship between spousal support and depression, anxiety, stress, and prenatal attachment in high-risk pregnant women. Materials and Methods: Data were collected from 375 high-risk pregnant women in their 2nd/3rd trimesters in November 2021-February 2022. An introductory information form, the Spouse Support Scale (SSS), the Depression, Anxiety, and Stress Scale (DASS-21), and the Prenatal Attachment Inventory (PAI) were used to collect data. Results: Median SSS score was 76 (29-81), median PAI score was 74 (31-84). Median anxiety scale score was 4 (0-21), depression scale score was 2 (0-21), and stress scales score was 3 (0-21). There was a positive significant relationship between spousal support and prenatal attachment, and a negative significant relationship between spousal support and anxiety, depression, and stress (p<0.001). Conclusion: Inadequate spousal support in high-risk pregnancies is a risk factor for prenatal attachment, anxiety, depression, and stress levels. Keywords: High risk pregnancy; Spousal support; Anxiety; Depression.","PeriodicalId":8248,"journal":{"name":"Annals of Medical Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73148046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}