Archives internationales de physiologie et de biochimie最新文献_第2页

Slight differences between adenosine deaminases from different species an immunochemical study. 不同物种的腺苷脱氨酶之间的细微差异:免疫化学研究。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009114004

J J Centelles, R Franco

引用次数: 0

Toxic impact of phosphamidon on protein degradation in phasic and tonic muscles of marine prawn, Penaeus indicus (H. Milne Edwards). 磷脂对印度对虾(Penaeus indicus)相肌和强直肌蛋白质降解的毒性影响。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009113996

M S Reddy, P R Kodavanti, K V Rao

{"title":"Toxic impact of phosphamidon on protein degradation in phasic and tonic muscles of marine prawn, Penaeus indicus (H. Milne Edwards).","authors":"M S Reddy, P R Kodavanti, K V Rao","doi":"10.3109/13813459009113996","DOIUrl":"https://doi.org/10.3109/13813459009113996","url":null,"abstract":"Levels of various protein fractions, (sarcoplasmic, myosin, actin, non-collagen and collagen) and the rate of their degradation by proteases were studied in phasic and tonic muscles of marine prawn, Penaeus indicus following acute (2 d) and chronic (15 d) exposure to sublethal concentration of phosphamidon. During exposure, greater decrease in sarcoplasmic protein fraction was observed in phasic muscle as compared to other myofibrillar proteins. But the sarcoplasmic protein content showed an elevation in tonic muscle. The changes in protein fractions were more pronounced during acute exposure than chronic exposure both in phasic and tonic muscles. These changes were correlated with the elevation of the acidic, neutral and basic protease activities during acute and chronic exposure. Free amino acids were increased during acute exposure, while they showed a significant decrease during chronic exposure in both the muscles. These results indicate that protein metabolism in both phasic and tonic muscles was significantly altered following phosphamidon exposure. These differential responses observed at acute and chronic exposure indicate the operation of compensatory mechanisms to mitigate the phosphamidon toxic stress.","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"98 6","pages":"347-51"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459009113996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Production of superoxide anion and hydrogen peroxide by KB cells in an anoxia-reoxygenation model, and role of allopurinol. 在缺氧-再氧化模型中KB细胞产生超氧阴离子和过氧化氢，以及别嘌呤醇的作用。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009114008

M Serhrouchni

引用次数: 2

Alterations in the physicochemical properties of renal cortical membranes in rats with experimental cirrhosis of the liver. 实验性肝硬化大鼠肾皮质膜理化性质的改变。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009113999

P Pérez-Rodrigo, F Gavilanes, J M López-Novoa

引用次数: 0

Effect of extracellular volume expansion on erythrocyte sodium transport in rats. 细胞外体积扩张对大鼠红细胞钠转运的影响。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009114006

N Hernando, R Bosch, M D Conesa, J M López-Novoa

{"title":"Effect of extracellular volume expansion on erythrocyte sodium transport in rats.","authors":"N Hernando, R Bosch, M D Conesa, J M López-Novoa","doi":"10.3109/13813459009114006","DOIUrl":"https://doi.org/10.3109/13813459009114006","url":null,"abstract":"The effects of extracellular volume expansion (EVE) on the major sodium transport systems and sodium and potassium contents in rat erythrocytes have been examined in the present study. Study has been performed in anesthetized Wistar rat weighing about 300 g. Acute extracellular volume expansion (EVE) was induced by a constant intravenous saline infusion (3% body wt, 3 hours). Rats anaesthetized and catheterized but not expanded were used as controls. Arterial blood samples from control and expanded rats were obtained at the same time, and assayed immediately. Intracellular sodium and potassium concentration and ouabain sensitive (Na(+)-K(+)-pump) and bumetanide sensitive (Na(+)-K(+)-cotransport system) outward Na+ fluxes in erythrocytes were measured. The effect of plasma on erythrocyte transport was also analyzed by measuring 86Rb uptake. Neither of two plasma cations (Na+ and K+) were modified by the EVE. Also intracellular Na+ and K+ levels remained unvariable. Total Na+ efflux was not modified by EVE, but pump-mediated Na+ efflux was smaller after than before EVE. The ouabain-inhibible Na+ efflux rate constant decreased after EVE (from 687 +/- 81 to 525 +/- 29 h-1 x 10(-3); P less than 0.05). Both Na(+)-K(+)cotransport-mediated Na+ efflux and passive permeability increased significantly after EVE. The incubation with plasma from saline-infused animals induced a significant decrease in Rb uptake rate constant, that was not observed after incubation with plasma from non-expanded rats.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"98 6","pages":"439-44"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459009114006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrical parameters of the toad skin: effects of forskolin. 蟾蜍皮肤的电参数:福斯克林的影响。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009113992

G A Castillo, A Coviello, G G Orce

{"title":"Electrical parameters of the toad skin: effects of forskolin.","authors":"G A Castillo, A Coviello, G G Orce","doi":"10.3109/13813459009113992","DOIUrl":"https://doi.org/10.3109/13813459009113992","url":null,"abstract":"Forskolin stimulated short-circuit current (SCC) and transepitelial electrical conductance (G) in the isolated skin of the toad Bufo arenarum in a concentration-dependent manner, between 1.0 x 10(-6) and 2.4 x 10(-5) M. At the latter concentration, glandular secretion appeared to be stimulated also. The increase in G was considerably greater in skins bathed in Ringer solution than in solutions containing no chloride. The increased SCC was abolished by amiloride, a specific blocker of sodium transport in amphibian membranes, irrespective of the anion present in the solution bathing the skin. G was also decreased by amiloride to control values in skins bathed in solutions without chloride, but remained elevated in the presence of Cl-. The increase in SCC following exposure to forskolin, 4.4 x 10(-6) M, was not altered when furosemide, a specific blocker of chloride transport, was present in the Ringer solution bathing the dermal side of the skin. The response to forskolin, 2.4 x 10(-5) M, however, was significantly decreased by dermal furosemide; the inhibitor was ineffective in the absence of chloride. The data indicate that forskolin acts on at least two sites: stratum granulosum cells (the main pathway for sodium transport, and an alternate site, responsible for the increase in permeability to chloride. In addition, at high concentration of the agent, glandular secretion is also stimulated. The data suggest that the adenylate cyclase-cyclic AMP system is involved in the regulation of the permeability of the toad skin to sodium and chloride, probably by separate cell types.","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"98 6","pages":"315-24"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459009113992","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12870570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Erucic acid metabolism in rat heart. A combined biochemical and radioautographical study. 大鼠心脏芥酸代谢。生化和放射自显影相结合的研究。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009114000

C Caselli, H Carlier, J Bezard

{"title":"Erucic acid metabolism in rat heart. A combined biochemical and radioautographical study.","authors":"C Caselli, H Carlier, J Bezard","doi":"10.3109/13813459009114000","DOIUrl":"https://doi.org/10.3109/13813459009114000","url":null,"abstract":"Metabolism of Erucic Acid was studied in rat heart in comparison with that of oleic acid, particularly in relation with diet lipids. Rats were fed for 3 or 60 days a diet containing 30% of the calories of either Rapessed Oil, rich in erucic acid or sunflower seed oil rich in linoleic acid. They were I.V. injected with tritiated erucic or oleic acid. After 1 or 15 min the radioactivity recovered in heart lipids was very low whatever the diet (1 to 2%). One minute after injection of erucic acid the radioactivity was mainly recovered in the free fatty acid fraction and as untransformed erucic acid. After 15 min the major part of radioactivity was recovered in the triacylglycerol fraction which contained a high proportion of labelled oleic acid formed by shortening of erucic acid. When oleic was injected, the radioactivity was principally recovered in triacylglycerols as untransformed oleic acid whatever the experimental conditions. Electron microscopy showed that a much higher proportion of peroxisomes, was present in heart cells, following sunflower seed oil diet as compared to rapeseed oil diet. In all cases mitochondria supported the greater part of radioactivity, especially when erucic acid was injected in rats fed rapeseed oil. After sunflower seed oil, a noticeable radioactivity was observed in peroxisomes, most of them containing silver grains, especially when oleic acid was injected. According to the data reported, peroxisomes do not seem more implicated than mitochondria in the metabolism of erucic acid in myocardium.","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"98 6","pages":"377-95"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459009114000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Action of cholecystokinin on the dog sphincter of Oddi: influence of anti-cholinergic agents. 胆囊收缩素对犬Oddi括约肌的作用:抗胆碱能药物的影响。

Archives internationales de physiologie et de biochimie Pub Date : 1990-12-01 DOI: 10.3109/13813459009113997

M J Pozo, G M Salido, J A Madrid

{"title":"Action of cholecystokinin on the dog sphincter of Oddi: influence of anti-cholinergic agents.","authors":"M J Pozo, G M Salido, J A Madrid","doi":"10.3109/13813459009113997","DOIUrl":"https://doi.org/10.3109/13813459009113997","url":null,"abstract":"Effects of cholecystokinin (CCK) on bile flow through the sphincter of Oddi (SO) were studied in anaesthetized dogs. Intravenous injection of CCK (0.25, 0.5, 1 and 2 IDU/Kg) elicited a dose-dependent reduction in flow through the SO in the first minutes after CCK administration. Pirenzepine and atropine decreased significantly (P less than 0.05) by 29% and a 40% respectively the inhibitory effect induced by 1 IDU/Kg of CCK, whereas hexamethonium elicited an increase in the inhibitory effect induced by 0.5 IDU/Kg of CCK (P less than 0.05). Intravenous infusion of cummulative doses of CCK had different effects according to the dose infused. Lower doses (0.025 and 0.05 IDU/Kg/min) increased transphincteric flow, however, high doses (0.1, 0.2 and 0.4 IDU/Kg/min) were inhibitory. These finding indicated that CCK had two effects on the SO : firstly, a contractile effect, probably mediated through a direct myogenic action and neuronal release of ACh, and secondly a relaxant effect, probably mediated by stimulation of inhibitory postganglionic neurons.","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"98 6","pages":"353-60"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459009113997","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Stressors and pain sensitivity in CFW mice. Role of opioid peptides. 应激源对CFW小鼠疼痛敏感性的影响。阿片肽的作用。

Archives internationales de physiologie et de biochimie Pub Date : 1990-10-01 DOI: 10.3109/13813459009113984

A M Konecka, I Sroczynska

引用次数: 23

Effects in vitro of mercury on rat brain Mg(++)-ATPase. 汞对大鼠脑Mg(++)- atp酶的体外影响。

Archives internationales de physiologie et de biochimie Pub Date : 1990-10-01 DOI: 10.3109/13813459009113986

C S Chetty, V McBride, S Sands, B Rajanna

{"title":"Effects in vitro of mercury on rat brain Mg(++)-ATPase.","authors":"C S Chetty, V McBride, S Sands, B Rajanna","doi":"10.3109/13813459009113986","DOIUrl":"https://doi.org/10.3109/13813459009113986","url":null,"abstract":"Mercuric chloride (Hg) in micromolar concentrations inhibited Mg(++)-dependent ATPase activity in rat brain microsomes. Inhibition was higher in oligomycin-sensitive (O.S.) than oligomycin-insensitive (O.I.) Mg(++)-ATPase. Hydrolysis of ATP with 15 and 50 micrograms of microsomal protein for 45 min without and with (2.10(-7M) Hg showed linear rates for 15-20 min. Altered pH vs activity demonstrated comparable inhibitions by Hg in buffered (neutral greater than acidic greater than basic) pH ranges. Inhibition of enzyme activity by Hg was found to be greater at 37 degrees C than at lower temperatures suggesting positive correlation trend. An uncompetitive inhibition with respect to the activation of Mg(++)-ATPase, O.S. Mg(++)-ATPase and O.I. Mg++ ATPase by ATP was indicated by a decrease in apparent Vmax and Km. Mg(++)-activation kinetic studies indicated that Hg causes uncompetitive inhibition of Mg(++)-ATPase and O.I. Mg(++)-ATPase and mixed inhibition of O.S. Mg(++)-ATPase. Inhibition was partially restored by repeated washings. These results indicate that the inhibition of microsomal Mg(++)-ATPase by Hg was pH, temperature, enzyme and Mg++ concentration dependent. Additionally, the data also suggest that O.S. compared to O.I. Mg(++)-ATPase is more sensitive to Hg toxicity.","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"98 5","pages":"261-7"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813459009113986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12873561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6