Florian Quinger, Nicolas Klein, Amélia Camarinha-Silva, Jana Seifert, Markus Rodehutscord
{"title":"Evaluation of pea grains using coupled <i>in vitro</i> assays for precaecal digestion and hindgut fermentation in pigs.","authors":"Florian Quinger, Nicolas Klein, Amélia Camarinha-Silva, Jana Seifert, Markus Rodehutscord","doi":"10.1080/1745039X.2025.2606085","DOIUrl":"10.1080/1745039X.2025.2606085","url":null,"abstract":"<p><p>The nutrient composition of the pea grain fraction that escapes precaecal digestion may vary in response to the pea variant, affecting its fermentation characteristics in the hindgut. Quantifying the formation of fermentation end products <i>in vivo</i> remains challenging. Therefore, this study investigated the digestion and fermentation characteristics of 12 pea variants using two combined <i>in vitro</i> assays. <i>In vitro</i> indigestible solids (IVIS) of pea grains, representing the fraction of pea grains escaping precaecal digestion, were incubated for 48 h using a modified Hohenheim gas test with pig faeces as inoculum. Gas production was recorded at nine time points, and NH<sub>4</sub><sup>+</sup>-N and volatile fatty acids (VFA) were measured at 8 and 24 h. Precaecal disappearance was high for starch and crude protein ( >94%), whereas fibre fractions remained largely undigested ( <16%) and were the most abundant and variable fraction in the IVIS. Incubated IVIS differed in potential gas production and maximum rate of gas production among pea variants (<i>p</i> < 0.001, 46.7 to 73.9 mL/200 mg dry matter and 2.9 to 5.1 mL/h, respectively). For metabolite concentrations, an effect of the IVIS was observed for NH<sub>4</sub><sup>+</sup>-N and VFA at 8 and 24 h (<i>p</i> < 0.001). Multivariate regression showed a positive association of acid detergent fibre with the potential gas production (<i>p</i> < 0.001, R<sup>2</sup> = 0.82). The concentration of crude protein was positively associated with the NH<sub>4</sub><sup>+</sup>-N concentration at 8 and 24 h (<i>p</i> < 0.001, R<sup>2</sup> = 0.85, R<sup>2</sup> = 0.86). The crude fibre concentration was negatively associated with the branched-chain fatty acid concentration at 24 h (<i>p</i> = 0.001, R<sup>2</sup> = 0.70). Starch concentration was positively associated with gas production at 2 h (<i>p</i> < 0.016, R<sup>2</sup> = 0.49) and propionate concentration at 8 h (<i>p</i> < 0.038, R<sup>2</sup> = 0.86). To conclude, early-stage propionate production is driven by the precaecal indigestible starch, whereas the fermentation potential is primarily determined by the precaecal indigestible fibre fractions. Formation of metabolites by protein fermentation depends on the concentration of precaecal indigestible protein but may be inhibited with higher fibre concentrations. The differences in fermentation characteristics among IVIS of pea variants were associated with the analysed nutrient composition of the IVIS but not with those of the original grains.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"35-49"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145987846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meta-analysis-based estimates of the efficiency of phosphorus utilisation by dairy cows.","authors":"K J Wild, E Haese, H Ahmadi, M Rodehutscord","doi":"10.1080/1745039X.2026.2639323","DOIUrl":"10.1080/1745039X.2026.2639323","url":null,"abstract":"<p><p>Phosphorus (P) has several vital functions, and its excretion may have environmental impacts. Thus, an adequate P supply is of utmost importance to meet the requirements of the animals while avoiding unnecessary excretion. Calculation of the P requirement of dairy cows considers factors such as P secretion via milk and P deposition in growing or pregnant animals. Furthermore, a value for the efficiency of P utilisation is applied, which should be determined in animals fed at a marginal P supply to maximise absorption. The objective of the present meta-analysis was thus to estimate the efficiency of P utilisation, accounting for P supply in dairy cows. The dataset included 399 observations published in 77 studies on dairy cows. Data on P intake and faecal P excretion were used to calculate standardised P digestibility, corrected for faecal endogenous P losses. Furthermore, the P requirement and the P supply relative to the requirement were calculated. Linear and non-linear regressions were performed to analyse the relationship between P excretion via faeces and urine and standardised P digestibility with P intake and P supply. Phosphorus intake exceeding the net requirement of dairy cows was excreted with faeces to a large extent, while urinary P excretion had only minor quantitative importance below 5.2 g P/kg DM of feed. The estimated standardised P digestibility at a marginal P supply was 83% and was interpreted as the estimate for efficiency of P utilisation. The data did not indicate that differentiation in P utilisation efficiency between mineral and organic P sources in the feed or by lactation stage is helpful for adequate P supply of dairy cows.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"67-86"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vasil Radoslavov Pirgozliev, Isobel Margaret Whiting, Stephen Charles Mansbridge, Kristina Kljak, Heloisa Helena de Carvalho Mello, José Henrique Stringhini, Amy E Johnson, Falko Drijfhout, David Yovchev, Atanas Georgiev Atanasov, Zhi Yang, Stephen Paul Rose
{"title":"The use of xylanase enzyme and inulin prebiotic supplementation to support broiler chicken production performance.","authors":"Vasil Radoslavov Pirgozliev, Isobel Margaret Whiting, Stephen Charles Mansbridge, Kristina Kljak, Heloisa Helena de Carvalho Mello, José Henrique Stringhini, Amy E Johnson, Falko Drijfhout, David Yovchev, Atanas Georgiev Atanasov, Zhi Yang, Stephen Paul Rose","doi":"10.1080/1745039X.2025.2604540","DOIUrl":"10.1080/1745039X.2025.2604540","url":null,"abstract":"<p><p>Two isoenergetic (12.05 MJ/kg ME) and isonitrogenic (approximately 200 g/kg CP) basal diets were prepared using 670 g/kg of wheat with low fibre and/or high fibre contents. Each basal diet was divided into four portions: one remained as is and fed as control (C); the second was the C supplemented with 100 FXU/kg of a commercial xylanase (XYL); the third was the C plus 20 g/kg of inulin (IN) powder; and the fourth was the C supplemented with both XYL and IN at the same inclusion rates, resulting in a total of eight experimental diets. A study was conducted from 10 to 21 days of age involving 320 female Ross 308 broiler chickens. Each diet, in meal form, was fed <i>ad libitum</i> to eight pens, five birds each, following randomisation. Supplementary XYL increased dietary nitrogen corrected apparent metabolisable energy (AMEn) (<i>p</i> < 0.001), dry matter retention (DMR), daily feed intake (FI), weight gain (WG) and reduced feed conversion ratio (FCR) (<i>p</i> < 0.05). Dietary IN tended (<i>p</i> < 0.01) to increase WG and reduce FCR and dietary wheat did not have an impact on growth performance variables (<i>p</i> > 0.05). Birds fed XYL had reduced relative weight of the pancreas (<i>p</i> < 0.050) and there was XYL by IN interaction (<i>p</i> < 0.05) on relative weight of the caeca as it was greater for those fed XYL and IN. No other changes in relative weight of the gastrointestinal tract organs were observed (<i>p</i> > 0.05). Bird fed XYL or IN had greater (<i>p</i> < 0.05) butyric acid (BA) concentration in caecal content. Feeding XYL led to reduced acetic (AA) and propionic acid (PA) concentrations in caecal excreta (<i>p</i> < 0.05) and to a greater BA:AA ratio (<i>p</i> < 0.001). Dietary IN increased blood serum glutathione peroxidase (GSH-Px) (<i>p</i> < 0.05) and XYL increased concentration of hepatic coenzyme Q<sub>10</sub> (<i>p</i> < 0.05). The type of dietary wheat did not have an impact on any of the studied variables, suggesting that birds were able to tolerate the fibre contents in this study. It seems that both IN and XYL, can serve as feed enhancers that potentially may promote antioxidant status of birds and help poultry to cope with various stress factors during production. The study further confirms that supplementing wheat-based diets with XYL may be a strategy to mitigate the reduction in available energy and to increase nutrient availability in broiler diets.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"1-16"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lena Rippstein, Natascha Titze, Katharina Judith Wild, Markus Rodehutscord
{"title":"Evaluation of sheep faeces as an alternative inoculum source to cow rumen fluid for estimation of ruminal ammonia-nitrogen release and microbially bound nitrogen <i>in vitro</i>.","authors":"Lena Rippstein, Natascha Titze, Katharina Judith Wild, Markus Rodehutscord","doi":"10.1080/1745039X.2025.2541805","DOIUrl":"10.1080/1745039X.2025.2541805","url":null,"abstract":"<p><p><i>In vitro</i> gas production (GP) techniques, such as the extended Hohenheim gas test (eHGT), are being used to estimate the protein values of ruminant feeds and commonly apply rumen fluid obtained from rumen-cannulated animals as inoculum. This study aimed to compare rumen fluid inoculum (RI) with faecal inoculum (FI) concerning <i>in vitro</i> ammonia-nitrogen (NH<sub>3</sub>-N) release and microbially bound nitrogen (mN). Rumen fluid was obtained from lactating dairy cows and faeces from adult wether sheep. Six feeds for ruminants, including rapeseed meal, soybean meal, sunflower meal, pea grain, maize grain, and rye grain, were incubated for 8, 24, and 48 h with and without an additional carbohydrate (CHO) source. NH<sub>3</sub>-N increased with the incubation time for all feeds and both inocula. For NH<sub>3</sub>-N values after 8, 24 and 48 h of incubation, the two-way interaction between feed and inoculum source was significant (<i>p</i> < 0.001). Across the six feeds, the NH<sub>3</sub>-N values did not differ between RI and FI after 8 h, whereas RI-NH<sub>3</sub>-N was 17% and 23% higher than FI-NH<sub>3</sub>-N after 24 and 48 h, respectively. For most feeds, the mN values decreased with RI as incubation time increased. In contrast, mN in FI remained constant or increased between 8 and 24 h before decreasing at 48 h. Overall, the NH<sub>3</sub>-N and mN values were lower with FI than RI. After 24 and 48 h, the two inocula did not significantly differ in the response to the CHO supply, studied as the ratio of mg NH<sub>3</sub>-N decrease per 1 mL of GP. Linear regression analysis showed that FI-NH<sub>3</sub>-N can be used to predict RI-NH<sub>3</sub>-N using the six feeds or the CHO-supplemented feeds at all incubation times. The highest estimation accuracy was found at 24 h for the feeds alone (slope = 1.39, R<sup>2</sup> = 0.98, CV = 3.9%) and the feeds plus CHO source (slope = 1.55, R<sup>2</sup> = 0.99, CV = 4.1%). In conclusion, FI has the potential to be applied as an inoculum to assess the protein values of ruminant feeds <i>in vitro</i>.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"171-186"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luciana D de Oliveira, Livio Penazzi, Iris M Kawauchi, Guilherme R Palumbo, Stephanie de S Theodoro, Liviana Prola, Aulus C Carciofi
{"title":"Digestiblity and metabolisable energy in high-ash poultry meal and plant protein ingredients for dogs.","authors":"Luciana D de Oliveira, Livio Penazzi, Iris M Kawauchi, Guilherme R Palumbo, Stephanie de S Theodoro, Liviana Prola, Aulus C Carciofi","doi":"10.1080/1745039X.2025.2584815","DOIUrl":"10.1080/1745039X.2025.2584815","url":null,"abstract":"<p><p>Using the substitution method and extruded diets, the metabolisable energy (ME) and digestible nutrient contents of soybean meal (SBM), poultry by-product meal with high ash content (PBM<i>ha</i>), micronised soybean meal (MSM), and corn gluten meal (CGM) were determined for dogs. A complete and balanced control diet (CD) was formulated. Test diets were prepared by mixing 70% of the CD with 30% of the protein source in evaluation, according to the substitution method requirements. Due to its high fat content, the MSM diet was formulated with 85% of the CD and 15% of MSM to obtain adequate extrusion. Thirty-five Beagle dogs (7 per diet) were used to evaluate the total tract apparent digestibility (TTAD) of each diet, by the total collection of faeces method. Results were applied to calculate the ingredient TTAD and digestible nutrients content. Data were analysed in a completely randomised design and submitted to ANOVA and Tukey test (<i>p</i> < 0.05). The TTAD of DM, OM, CP, and gross energy were higher for CGM (<i>p</i> < 0.05). The TTAD of CP was similar for SBM and MSM, whose values were higher than for PBM<i>ha</i> (<i>p</i> < 0.05). Faeces production was higher for dogs fed the SBM diet and PBM<i>ha</i>, and faecal DM was lower for dogs fed the SBM diet. The results indicate variable TTAD among protein sources, with vegetable protein sources generally being more digestible than PBM<i>ha</i>.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"234-247"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145572770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Water intake and drinking behaviour in Holstein, Gyr and Girolando-F1 cows presenting high body condition score during the transition period.","authors":"Sheila Cristina Bosco Stivanin, Elissa Forgiarini Vizzotto, Arthur Fernandes Bettencourt, Anne Rosi Guadagnin, Júlia Fernandes Aires, Fernanda Samarini Machado, Mariana Magalhães Campos, Thierry Ribeiro Tomich, Luiz Gustavo Ribeiro Pereira, Vivian Fischer","doi":"10.1080/1745039X.2025.2586092","DOIUrl":"10.1080/1745039X.2025.2586092","url":null,"abstract":"<p><p>Water consumption may vary depending on climate, dry matter intake (DMI) as well as genetic group and physiological state of the cows. Little is published for other genetic groups besides Holstein. Understanding the need for water intake (WI) as well as drinking behaviour in critical periods could help producers develop adjusted management practices. This study aimed to investigate the drinking and water consumption behaviour of Holstein, Gyr and Girolando-F1 (½ Holstein ½ Gir) cows during the transition period. Thirty-six cows were distributed into three groups (<i>n</i> = 12 each) according to their genetic group. WI and water drinking behaviour were monitored from 10 days before parturition until 21 days of lactation using electronic drinkers. During <i>pre</i>-calving, a positive correlation was observed between WI and DMI only for Holstein (<i>r</i> = 0.31; <i>p</i> < 0.05) and Girolando-F1 (<i>r</i> = 0.34; <i>p</i> < 0.05) cows. Holstein cows ingested the highest amount of water daily and per 100 kg of body weight (BW) (30.9 kg and 4.5 kg). Holstein cows had higher WI per BW<sup>0.75</sup> than Gyr cows (0.23 kg vs. 0.12 kg). Furthermore, Holstein cows remained longer and spent more active time at the drinker (85.4 min; 81.2 min) than Gyr (13.5 min; 12.1 min) and Girolando-F1 cows (36.8 min; 34.4 min; <i>p</i> < 0.05), respectively. Holstein and Girolando-F1 cows had a higher daily number of active visits to the drinker than Gyr cows (4.2, 4.2, and 2.8 times, respectively). In the <i>post</i>-calving period, WI was positively correlated with DMI in all genetic groups, but it was correlated with milk yield (MY) only in Holstein (<i>r</i> = 0.21; <i>p</i> < 0.05) and Girolando-F1 (<i>r</i> = 0.42; <i>p</i> < 0.05) cows. The WI per MY and per BW<sup>0.75</sup> was lower for Girolando-F1 cows in relation to the Holstein and Gyr cows on the first day <i>post</i>-calving and highest for Gyr cows on the seventh day <i>post</i>-calving. WI per kg of BW<sup>0.75</sup> was higher for Holstein cows than for Gyr cows during the first 21 days of lactation, while WI per kg BW<sup>0.75</sup> of Holstein cows was higher than that of Girolando-F1 cows on days 3 and 4 and from days 7 to 21. The genetic groups differed in WI and drinking behaviour and were mainly modulated by DMI and THI in the <i>pre</i>-calving period and by MY and DMI <i>in the post</i>-calving period.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"248-266"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145627916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Sofía Castaño Ledesma, Gustavo Adolfo Palma, Claudio Darío Borsarelli, María Sumampa Coria
{"title":"Post-mortem oxidative status and gene expression of antioxidant enzymes in <i>longissimus thoracis et lumborum</i> muscle of Braford steers with different feeding systems.","authors":"María Sofía Castaño Ledesma, Gustavo Adolfo Palma, Claudio Darío Borsarelli, María Sumampa Coria","doi":"10.1080/1745039X.2025.2581372","DOIUrl":"10.1080/1745039X.2025.2581372","url":null,"abstract":"<p><p>Meat is highly susceptible to oxidation, which leads to the degradation of lipids and proteins, resulting in undesirable changes in meat quality, such as colour and shelf life. This process can be mitigated by both non-enzymatic antioxidants and enzymatic antioxidants, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). The oxidative stability of meat is influenced by several factors, including animal nutrition. The aim of this study was to assess the impact of corn silage supplementation on meat oxidative status and its correlation with the expression level of genes associated with this process in the <i>longissimus thoracis et lumborum</i> muscle of Braford steers. For this study, 30 steers were randomly assigned to two experimental groups of 15 animals each. One group was fed with <i>ad libitum</i> pasture supplemented with corn silage and the other group was fed with <i>ad libitum</i> pasture. The degree of protein and lipid oxidation was determined by total protein carbonyl content and lipid degradation products, respectively. The antioxidant capacity was determined by measuring the radical-scavenging activities, total phenol content (TPC), and the activities of the CAT, SOD, and GPx enzymes. In addition, the relative levels of <i>sod</i> (1 and 2), <i>cat</i>, and <i>gpx</i> genes were evaluated using real-time polymerase chain reaction. The results indicated that meat from corn-silage supplementation exhibited higher levels of oxidation than those from pasture-fed steers. Also, results showed that meat from corn-silage-supplemented steers were more oxidised than those from pasture-fed steers. Moreover, hydrophilic extracts showed higher levels of antioxidant capacity than liphophilic ones in boths feeding systems and the muscles of animals that received corn exhibited a 17% reduction in TPC. In addition, expression levels of <i>cat, sod</i> (1 and 2), and <i>gpx</i> genes were lower in this group compared to the pasture-finished group. Consequently, the gene expression of the antioxidant enzymes exhibited a negative correlation with lipid oxidation, and with lipophilic antioxidants. Conversely, a positive correlation was observed with polyphenol content. This study demonstrated that the oxidative stability of meat can be enhanced by feeding steers with pasture, rather than finishing with corn silage supplementation. Moreover, attributable to reduced expression levels of antioxidant enzymes, leading to higher lipid and protein oxidation levels, which has a deleterious effect on meat quality. Consequently, the gene expression of the antioxidant enzymes evaluated, specifically <i>sod, cat and gpx</i>, has been identified as a promising reliable biomarker for oxidative stability in meat.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"219-233"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145487550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kirsten Schulz, Bettina Wagner, Holger Sieg, Maciej Durkalec, Tom Heinze, Tanja Schwerdtle, Markus Spolders, Robert Pieper
{"title":"Influence of copper level and source on performance, tissue accumulation, and faecal excretion in fattening pigs.","authors":"Kirsten Schulz, Bettina Wagner, Holger Sieg, Maciej Durkalec, Tom Heinze, Tanja Schwerdtle, Markus Spolders, Robert Pieper","doi":"10.1080/1745039X.2025.2541983","DOIUrl":"10.1080/1745039X.2025.2541983","url":null,"abstract":"<p><p>Copper (Cu) supplementation is essential in pig nutrition; however, its effects on performance, trace element accumulation in edible tissues, and environmental excretion require careful evaluation. In the present study a total of 24 male, castrated fattening pigs of two different hybrid mast lines (11 weeks of age) were divided according to their initial body weight (25.8 ± 3.5 kg) into four groups. Pigs were fed for 14 weeks a complete feed supplemented with Cu covering a range between the recommended Cu supplementation and the permissible European maximum level (i.e. 5, 15, and 25 mg/kg complete feed). Two different Cu sources were used: Cu sulphate (CuSO₄) and glycine-Cu chelate hydrate (Cu-Gly). The aim of the trial was studying the influence of the different Cu levels and sources on growth performance, Cu transfer to edible tissues (muscle, liver, kidney, blood) and faeces, as well as the concentration of other trace elements, including iron (Fe), zinc (Zn) and manganese (Mn) in tissues of fattening pigs. Performance parameters, including average daily gain (ADG) and feed conversion ratio (FCR), showed a dependency with respect to the pig breed, whereas Cu content and Cu sources showed no influence. Copper concentrations in the liver, kidney, muscle, and blood serum remained constant across groups. Faecal Cu excretion increased proportionally with dietary Cu levels, with higher excretion observed for the organic Cu source at 15 mg/kg as compared with inorganic source at similar level. The results show, that Cu levels up to the maximum level of 25 mg/kg complete feed are not necessary to achieve good growth performance in healthy fattening pigs. The unaffected Cu concentrations in liver, muscle, kidney, and blood, as well as increased faecal excretion with increasing Cu level in the feed, indicate an adequate supply and homoeostatic regulation of Cu. In addition, a reduced use of Cu in pig fattening will help to reduce Cu emission into the environment.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"187-202"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yasir Afzal Beigh, Haidar A Ahmad, Zulfiqarul Haq, Sheikh R Ahmad, Showkat Shah, Sheikh Adil, Sireen A R Shilbayeh, Gamal A El-Shaboury
{"title":"Evaluation of walnut oilcake as a sustainable substitute to conventional feedstuffs for broiler chickens: effect on production performance, intestinal health, carcass and meat quality traits.","authors":"Yasir Afzal Beigh, Haidar A Ahmad, Zulfiqarul Haq, Sheikh R Ahmad, Showkat Shah, Sheikh Adil, Sireen A R Shilbayeh, Gamal A El-Shaboury","doi":"10.1080/1745039X.2025.2562825","DOIUrl":"10.1080/1745039X.2025.2562825","url":null,"abstract":"<p><p>Oil extraction from walnut (<i>Juglans regia</i> L.) kernels yields residue of walnut oilcake (WOC) that is often discarded as waste despite its rich bioactive contents. This study evaluates the potential use of WOC as a replacement for conventional feedstuffs in broiler diets, assessing its effects on production performance, nutrient utilisation, carcass traits, meat quality and gut health. A total of 160 day-old male broiler chicks in total were evenly distributed into four dietary groups, with five replications each consisting of eight chicks. The birds in group T0 were fed the control diet based on conventional corn-s oybean meal, while those of treatment groups were fed diets with WOC substituted for soybean meal at levels of 10% (T1), 20% (T2), and 30% (T3). The experiment lasted for 42 days. Inclusion of WOC upto 20% did not influence feed intake, early growth, FCR (until T2), or nutrient digestibility but inhibited performance at T3. Dressing yield and breast/thigh weights were improved (<i>p</i> < 0.05), whereas fat, cholesterol, TBARS, tyrosine, and drip loss decreased ;(<i>p</i> < 0.05) with increased antioxidant activity, water-holding capacity and meat lightness. WOC diets also decreased caecal coliforms and improved ileal villus morphology. In conclusion, WOC can replace soybean meal upto 20% without compromising production performance, while improving carcass traits, meat quality, oxidative stability and gut health in broiler chickens.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"203-218"},"PeriodicalIF":1.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Ali Ketata, Marie-Pierre Létourneau-Montminy, Frédéric Guay
{"title":"Impact of xylanase and protease supplementation on digestibility in growing pigs fed diets with varying levels of zinc and copper.","authors":"Mohamed Ali Ketata, Marie-Pierre Létourneau-Montminy, Frédéric Guay","doi":"10.1080/1745039X.2025.2542149","DOIUrl":"10.1080/1745039X.2025.2542149","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of dietary copper (Cu) and zinc (Zn) levels and xylanase and protease supplementation on apparent digestibility of nutrients at the end of the ileum (AID) and large intestine (ALID). Using a 2 × 2 factorial design, 24 weaned pigs were assigned to one of the four barley-wheat-soybean meal diets, supplemented with two levels of Cu/Zn (20/125 and 40/250 mg/kg) and either a mix of xylanase and protease (ENZ) or no enzyme supplementation. Neither the Cu/Zn level nor ENZ affected AID of nutrients. However, ALID of dry matter and fibres (ADF and NDF) increased with ENZ supplementation (<i>p</i> < 0.010). Enzyme supplementation also influenced the ALID of crude protein and phosphorus, depending on the Cu/Zn levels (Mineral × ENZ, <i>p</i> < 0.050). Specifically, ENZ increased the ALID of crude protein in the high Cu/Zn diet, while ALID of phosphorus increased by 63% with ENZ in low Cu/Zn diet but only by 23% in the high Cu/Zn diet. The high Cu/Zn level increased the ALID of dry matter but had no effect on the ALID of other nutrients. In conclusion, ENZ supplement increased the apparent digestibility of nutritional compounds, but this effect was dependent on the level of Cu/Zn in the diet. Further research is needed to explore the interaction between xylanase/protease and nutritional components of feed to maximise the benefits of these supplements.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":" ","pages":"155-169"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}