Aquacultural Engineering最新文献

{"title":"Site selection for off-shore macroalgae aquaculture on the French Atlantic coast","authors":"Graciela Patricia Medina Madariaga ,&nbsp;Mathilde Falcou-Prefol ,&nbsp;Simon Oiry ,&nbsp;Maria Laura Zoffoli ,&nbsp;Joël Fleurence ,&nbsp;Pierre Gernez ,&nbsp;Laurent Barillé","doi":"10.1016/j.aquaeng.2025.102581","DOIUrl":"10.1016/j.aquaeng.2025.102581","url":null,"abstract":"<div><div>Macroalgal aquaculture production has significantly increased worldwide in recent decades. In Europe, seaweed aquaculture is not yet well established, necessitating strategic planning for its development. In this study, the suitability for the offshore cultivation of three macroalgae (<em>Laminaria digitata</em>, <em>Saccharina latissima, and Palmaria palmata</em>) along the French North Atlantic coast was determined with a Spatial Multi-Criteria Evaluation (SMCE) process based on a Geographical Information System (GIS) workflow. Variables influencing macroalgal growth, such as nutrient concentration, sea temperature, water transparency, sea current speed and wave height, were selected and tailored to the specific requirements of each species. Environmental data were obtained from satellite remote sensing and model outputs. In addition, other socio-economical activities conflicting with aquaculture space were included within the SMCE and integrated into a global suitability index. This study showed that large areas in the French North Atlantic coast are suitable for offshore seaweed aquaculture but not for all months of the year. The strongest constrictions to aquaculture of <em>L. digitata</em>, <em>S. latissima</em> and <em>P. palmata</em> were caused by wave height and current speed. Among the studied species, <em>S. latissima</em> showed the largest suitable area for development (up to 1847 km²), while <em>P. palmata</em> presented the smallest area with 461 km². The suitability index showed a seasonal pattern, with higher values between November and April and optimal conditions in December. A sensitivity analysis revealed that temperature and nitrate concentration changes significantly impacted the total suitability index. Further validation based on experimental growth assessment is a perspective the aquaculture sector considers.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102581"},"PeriodicalIF":3.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of flow field and dissolved oxygen concentration distribution in aquaculture tanks based on computational fluid dynamics","authors":"Zhixin Xiong ,&nbsp;Ming Ma ,&nbsp;Yu Guo ,&nbsp;Yu Sun","doi":"10.1016/j.aquaeng.2025.102577","DOIUrl":"10.1016/j.aquaeng.2025.102577","url":null,"abstract":"<div><div>This paper investigated flow velocity distribution within aquaculture tanks (17 m×18 m×19 m) under various circulation rates. The model was based on an actual well boat primarily used for farming high-value species, including Atlantic salmon (Salmo salar). A three-dimensional turbulence model and an oxygen transport model, were employed as analytical tools to evaluate the spatial distribution of dissolved oxygen and assess environmental conditions for fish growth. The effects of key factors, such as total oxygen supply, theoretical oxygen consumption, and outlet flow velocity, affecting the distribution of dissolved oxygen concentration in the tank were also analyzed. A 1:15 scaled physical experiment was conducted based on Reynolds similarity. The results indicated that adjusting the inlet pipes position and moderately increasing oxygen supply has significantly positive effects on the uniformity of flow velocity and dissolved oxygen distribution in the tank. Furthermore, the overall dissolved oxygen concentration was linearly related to the difference between oxygen supply and consumption. When the oxygen supply was insufficient, increasing the circulation was beneficial to improve the ability of tanks to maintain dissolved oxygen. This study provides insights for the design of aquaculture tanks and strategies for oxygen supply at different stages of production.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102577"},"PeriodicalIF":3.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid H2S production in layers of freshwater and marine fish organic waste from recirculating aquaculture systems","authors":"Alexandre M. Nguyen-tiêt,&nbsp;Carlos O. Letelier-Gordo,&nbsp;Sanni L. Aalto","doi":"10.1016/j.aquaeng.2025.102578","DOIUrl":"10.1016/j.aquaeng.2025.102578","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S) is an extremely toxic gas that has been associated with fish mass mortality events in recirculating aquaculture systems (RAS), resulting in substantial economic losses for the aquaculture industry. Previous studies have identified favorable conditions and potential hotspots for H₂S production in RAS under mixed reactors conditions, but knowledge on temporal and spatial dynamics of H₂S production within layers of accumulated organic matter within these systems remains limited. In this study, we investigated H₂S production dynamics at fine spatial (millimeter) and temporal (hourly) scales in accumulated marine and freshwater organic waste collected from RAS, using a high-resolution microsensor setup. In addition to measuring the natural H₂S production potential, we examined the response to the addition of readily available carbon sources and sulfur-rich amino acid cysteine, as well as the microbial communities present in organic waste. In both freshwater and marine organic waste, H₂S appeared only within one centimeter of the accumulated fish organic waste after oxygen was consumed. When this organic waste was disturbed, the accumulated H₂S was rapidly released into the water column and reached concentrations above the toxicity threshold for fish. Through a combination of carbon and cysteine supplementation and 16S rRNA gene analysis, we identified cysteine degradation and sulfate reduction as key microbial pathways for H₂S production in the accumulated organic waste. Overall, this study demonstrates that hydrogen sulfide can form within very thin layers of accumulated fish organic waste in both freshwater and saltwater environments and once released, can reach toxic levels for fish. Therefore, it is crucial to focus on minimizing organic matter accumulation in RAS and implementing contingency measures when the bottom of tanks is disturbed.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102578"},"PeriodicalIF":3.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of filtration and horizontal substrate on Pacific White Shrimp (Litopenaeus vannamei) production and water quality in RAS","authors":"Gyanu Rana,&nbsp;Andrew J. Ray","doi":"10.1016/j.aquaeng.2025.102576","DOIUrl":"10.1016/j.aquaeng.2025.102576","url":null,"abstract":"<div><div>To produce high-quality, fresh shrimp near consumer markets in a sustainable fashion, recirculating aquaculture systems (RAS) can be employed. This project assessed the effects of two types of RAS: clear water (CW) and hybrid (HY), along with horizontal substrate, on intensive Pacific White Shrimp production and water quality. In CW RAS a very clean environment is created using a series of filters, but the technique may have relatively high equipment costs. More simplistic HY systems have external biofilters to cycle nutrients, but solids filtration is relatively minimal. Horizontal substrate may help to enhance shrimp production by increasing the surface area for shrimp to graze and escape competition. In this study, two levels of each experimental factor were used: system type (CW vs HY) and systems with substrate (WS) versus those with no substrate (NS). There were four treatments: HY-WS, HY-NS, CW-WS, and CW-NS, each of them with four replicates randomly assigned to 1-m³ circular tanks. Shrimp with an average weight of 1.3 g were stocked at a density of 450 m⁻³ and reared for 53 days. The HY-WS treatment resulted in significantly higher individual shrimp weight, total biomass harvested (7 kg m⁻³), survival, and growth rate compared to the HY-NS and CW-NS treatments. The CW-WS treatment was not significantly different than HY-WS or HY-NS treatments with regard to these parameters. TAN and nitrite-N were significantly lower in HY systems and those systems that contained substrate. The CW-NS treatment had a very high nitrite-N concentration. This resulted in low survival and may have been due to excess ozone entering the biofilters in the CW treatments and killing the nitrifying bacteria. Findings from this study suggest that inclusion of horizontal substrate in hybrid-style systems can significantly enhance production and help in biofiltration, but care should be taken with regard to disinfection measures.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102576"},"PeriodicalIF":3.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of aerobic digestion conditions of solid waste from recirculating aquaculture systems for liquid fertilizer production","authors":"Lukáš Harabiš ,&nbsp;Michal Šorf ,&nbsp;Marija Radojičić ,&nbsp;Jan Mareš","doi":"10.1016/j.aquaeng.2025.102574","DOIUrl":"10.1016/j.aquaeng.2025.102574","url":null,"abstract":"<div><div>Recycling nutrients from aquaculture sludge can contribute to more sustainable production systems and support circular economy approaches. This study evaluated aerobic digestion as a treatment strategy for solid organic waste generated in recirculating aquaculture systems (RAS) for African catfish (<em>Clarias gariepinus</em>), with the aim of optimizing operational conditions for maximum nutrient mineralization and recovery. A multifactorial experiment was conducted using four treatments (control, pH 6, pH 7, bacterial inoculation), each tested at three temperatures (20, 25, 30 °C) in triplicate (36 reactors in total). Each reactor was loaded with 10 g of freeze-dried sludge and 1 L of water or phosphate buffer. Digestion proceeded for 21 days. The most effective condition was the control treatment at 30 °C without pH adjustment or bacterial inoculation. Under this setting, the final nitrate concentration reached 81.4 mg/L. Phosphorus recovery efficiency (NRE) was 61.1 %, and potassium recovery reached 76.8 %. The final nutrient solution had a favorable NO₃⁻:NH₄⁺ ratio of 75:25. Higher temperature promoted nitrification and overall mineralization. Acidified conditions (pH 6) enhanced ammonium and phosphorus solubilization but slowed nitrate formation. The addition of external bacteria did not improve mineralization efficiency, likely due to microbial competition or limited inoculum performance. Heavy metals were below detection limits. However, persistent nitrite concentrations (∼4–5 mg/L) and poor solubilization of micronutrients, especially Fe and Zn, remain limitations. Laboratory-scale pretreatment (freeze-drying and milling) may have improved solubility compared to raw sludge and should be considered when interpreting results. The results suggest that optimized aerobic digestion of RAS sludge can support efficient nutrient recovery and may serve as a practical approach for producing liquid fertilizer applicable in hydroponics.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102574"},"PeriodicalIF":3.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow-through and cyclic fed-batch cultivation of the green microalga Haematococcus pluvialis in recirculating aquaculture effluent: Strategies for sustained biomass production and astaxanthin biosynthesis","authors":"Hemanta Timilsina ,&nbsp;Minna Hiltunen ,&nbsp;Marco L. Calderini ,&nbsp;Pauliina Salmi ,&nbsp;Juhani Pirhonen ,&nbsp;Katja Pulkkinen","doi":"10.1016/j.aquaeng.2025.102573","DOIUrl":"10.1016/j.aquaeng.2025.102573","url":null,"abstract":"<div><div>Integrating microalgal cultivation with recirculating aquaculture systems (RAS) offers a sustainable solution for nutrient recovery and the production of high-value bioproducts. The conventional batch cultivation technique for microalgae is constrained by limited biomass yields and short cultivation cycles. This study investigated the potential of cultivating <em>Haematococcus pluvialis</em> in RAS effluent and assessed flow-through and cyclic fed-batch cultivation strategies to enhance biomass and astaxanthin production. Both cultivation modes were investigated in two separate laboratory-scale experiments. Experiment I used (i) synthetic medium (modified BG-11) and (ii) untreated RAS effluent, while Experiment II used (i) autoclaved and (ii) filtered-autoclaved RAS effluent. Light stress was applied to the outflow from flow-through and the withdrawn culture from cyclic fed-batch cultivation to stimulate astaxanthin biosynthesis. In both experiments, the flow-through mode consistently outperformed the cyclic fed-batch mode in growth rate, cell density, biomass concentration, and nutrient uptake. In Experiment I, untreated RAS effluent supported <em>H. pluvialis</em> growth; however, microbial interference during the post-growth phase affected astaxanthin synthesis. In Experiment II, using pretreated RAS effluent, the flow-through mode achieved a maximum growth rate of 0.39 ± 0.01 day⁻¹ , nitrate removal of 64.28 ± 1.84 % and ∼100 % phosphate removal. The cyclic fed-batch mode achieved a maximum growth rate of 0.34 ± 0.01 day⁻¹ , nitrate removal of 55.05 ± 6.25 % and ∼100 % phosphate removal. Although, astaxanthin content was similar between modes (6.30 ± 0.48 mgg⁻¹ DW), the higher biomass concentration in flow-through mode resulted in a higher astaxanthin concentration (2.96 ± 0.78 mgL⁻¹) compared to cyclic fed-batch mode (1.6 ± 0.31 mgL⁻¹).</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102573"},"PeriodicalIF":3.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of artificial intelligence in aquaculture – Recent developments and prospects","authors":"Subha M. Roy ,&nbsp;Mirza Masum Beg ,&nbsp;Suraj Kumar Bhagat ,&nbsp;Durga Charan ,&nbsp;C.M. Pareek ,&nbsp;Sanjib Moulick ,&nbsp;Taeho Kim","doi":"10.1016/j.aquaeng.2025.102570","DOIUrl":"10.1016/j.aquaeng.2025.102570","url":null,"abstract":"<div><div>Artificial intelligence (AI) offers innovative and efficient solutions to contemporary challenges in sustainable aquaculture. Machine learning (ML) and deep learning (DL) are integral components of smart aquaculture, driving significant advancements in the field. The integration of AI with ML, and DL technologies is transforming traditional aquaculture practices by enhancing operational efficiency, optimizing fish health management, improving environmental conditions, monitoring water quality and supporting advanced decision-making processes. This review highlights the latest applications of AI, including ML, and DL in aquaculture, emphasizing their roles in real-time water quality monitoring, disease detection, and automated estimation of fish biomass etc. Key techniques, including predictive modeling, image and video processing, and sensor data integration, are enabling these breakthroughs. Moreover, DL algorithms, such as convolutional neural networks (CNNs) and long short-term memory (LSTM) networks, have emerged as powerful tools for processing complex data and predicting critical events within aquaculture systems. Despite the notable progress, challenges such as the need for large, labeled datasets, high computational costs, and issues related to model interpretability continue to limit broader adoption. The current review aims to offer researchers and practitioners with a comprehensive overview of AI and its subfields such as ML and DL applications in smart aquaculture, discussing both the opportunities and challenges while suggesting future research directions to overcome existing limitations and expand AI-driven innovations in the industry.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102570"},"PeriodicalIF":3.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computation and analysis of phenotypic parameters of Scylla paramamosain based on YOLOv11-DYPF keypoint detection","authors":"Chong Wu ,&nbsp;Shengmao Zhang ,&nbsp;Wei Wang ,&nbsp;Zuli Wu ,&nbsp;Shenglong Yang ,&nbsp;Wei Chen","doi":"10.1016/j.aquaeng.2025.102571","DOIUrl":"10.1016/j.aquaeng.2025.102571","url":null,"abstract":"<div><div>Phenotypic parameter measurement of mud crabs is a critical component in aquaculture and scientific research, serving as a key basis for assessing their growth status and quality. To enhance the efficiency of measuring growth parameters such as body dimensions and weight, this study employed the YOLOv11 model for carapace keypoint detection in mud crabs and automated calculation of body length and weight based on detection results. To improve model accuracy, we proposed two modifications to the standard YOLOv11 architecture: a self-developed multi-scale fusion module (SPPF-MP2) replacing the original SPPF module and a lightweight dynamic upsampling module (Dysample) substituting the conventional Upsample module. Results demonstrated that both modules individually contributed to performance enhancement, with a significant 1.8 % improvement in mAP@50–95 and a 0.1 % increase in mAP@50 when combined. During inference, pixel distances between the 12 annotated keypoints were leveraged to compute seven critical body dimensions, with relative errors below 10 % for all measurements. Furthermore, we established seven predictive models to correlate crab dimensions with weight. Among these, the Support Vector Regression (SVR) model exhibited the highest accuracy, achieving a maximum prediction error of 13.74 % and an average error of 4.90 %. This study confirms that YOLO-based visual models for keypoint detection enable high-precision estimation of body dimensions and weight in mud crabs, significantly streamlining the statistical analysis of growth parameters in aquaculture and ecological studies.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102571"},"PeriodicalIF":3.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrodynamics of large-scale recirculating aquaculture tanks equipped with paddlewheel aerators","authors":"Jun Zhang ,&nbsp;Yujun Zhang ,&nbsp;Jun Guo ,&nbsp;Zhuoling Liu ,&nbsp;Shouqi Cao ,&nbsp;Xingguo Liu","doi":"10.1016/j.aquaeng.2025.102569","DOIUrl":"10.1016/j.aquaeng.2025.102569","url":null,"abstract":"<div><div>In the pond recirculating aquaculture system (PRAS), the paddlewheel aerator is vital for improving dissolved oxygen and hydrodynamic conditions in recirculating aquaculture tanks (RATs). Understanding its influence on the hydrodynamic characteristics of RATs can enhance energy efficiency, improve sewage collection, and ensure uniform dissolved oxygen (DO) distribution. This study focuses on a 15 m side length chamfered RAT in a partitioned PRAS. The flow numerical model was validated by conducting a series of comparative analyses with the scale test model. Hydrodynamic parameters include flow uniformity, velocity, vorticity, Froude number, and effective energy utilization coefficient were analyzed. The Pearson correlation coefficient method explored the relationships between the effective energy coefficient and the aerator's placement angle and distance. Results indicate that increasing the aerator's angle and distance raises flow velocity and strengthens water mixing, promoting settleable particle discharge. But when the distance is <em>L</em>/4 of the RAT side length and the angle exceeds 30°, a low-velocity turbulent zone forms, blocking waste particle discharge. When the distance is <em>L</em>/6 or <em>L</em>/4 and the angle ranges from 20° to 30°, energy utilization and water mixing performance improve remarkably, increasing suspended particle discharge rate. This study offers a theoretical basis for enhancing large-scale RAT operation efficiency and water quality regulation ability.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102569"},"PeriodicalIF":3.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary investigation on replacing fish meal with integrated multi-trophic aquaculture produced bioflocs in the diets of advanced Red drum (Sciaenops ocellatus, Actinopterygii) juveniles","authors":"Nicholas Romano ,&nbsp;Paul S. Wills ,&nbsp;Juan F. Paredes ,&nbsp;Marty Riche ,&nbsp;Zachary Nilles","doi":"10.1016/j.aquaeng.2025.102566","DOIUrl":"10.1016/j.aquaeng.2025.102566","url":null,"abstract":"<div><div>Biofloc technology is a microbially-based water quality strategy in which bacteria transform otherwise toxic ammonia and nitrite into nutritious biomass that may replace proteins in fish diets. Biofloc meal (BM) produced from an <em>ex-situ</em> bioreactor as part of a land-based integrated multi-trophic aquaculture system (LB-IMTA) was incorporated into five isonitrogenous diets consisting of, 1) fish meal (FM)-based control (no BM), 2) BM replacing FM at 25 % (BM25), 3) BM25 with an amino acid (AA) mixture (methionine, lysine, leucine, threonine, and arginine), 4) BM replacing FM at 50 % (BM50), and 5) BM50 with the AA mixture. These diets were fed to Red drum (<em>Sciaenops ocellatus</em>) advanced juveniles (initial weight of 61.5 g) for 8 weeks. Fish fed the BM25 diet had similar growth and feeding efficiency compared to the control, while those fed the BM25AA diet led to significantly faster growth and better feeding efficiency than the control. Fish fed the BM50 experienced negative growth values. While the BF50AA diet had numerically worse growth and feeding efficiency, these values were not significantly different than the control. Whole-body lipid content in fish fed the BM50 and BM50AA diets was significantly lower than the other treatments. Results demonstrate that BM, as a by-product from LB-IMTA, can replace FM without AA supplementation in the diets of advanced Red drum juveniles. Remarkably, replacing FM by 25 % with BM when the AA mixture was included significantly improved Red drum growth compared to those fed the FM-based control diet. These findings demonstrate that BM with AA inclusions may not only improve production but potentially decrease feeding costs while enhancing sustainability in closed recirculating systems. Further research on enhancing the nutritional value of LB-IMTA produced BM may be a worthwhile direction to take advantage of a readily available by-product.</div></div>","PeriodicalId":8120,"journal":{"name":"Aquacultural Engineering","volume":"111 ","pages":"Article 102566"},"PeriodicalIF":3.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}