Applied Soil Ecology最新文献

{"title":"Urban forest soil properties and microbial characteristics: seasonal and stand-specific variations","authors":"Xin Wan ,&nbsp;Sumei Qiu ,&nbsp;Runyang Zhou ,&nbsp;Liwen Li ,&nbsp;Wei Xing ,&nbsp;Yingdan Yuan","doi":"10.1016/j.apsoil.2025.105995","DOIUrl":"10.1016/j.apsoil.2025.105995","url":null,"abstract":"<div><div>Urban forests are essential to maintain the stability of urban ecosystems. Soil microorganisms in forest environments are critical components of these ecosystems and are subject to various influencing factors. This study examined six distinct forest soils in the Zhuyu Bay Scenic Area of Yangzhou City, Jiangsu Province, China. The forest types investigated were mixed pine and cypress forest (PC), <em>Metasequoia glyptostroboides</em> (MG), <em>Cornus officinalis</em> (CO), mixed broad-leaved shrub forest (MS), mixed broad-leaved tree forest (MT), and bamboo forest (BF). The research involved measuring soil physicochemical properties, microbial biomass, and soil enzyme activity across four seasons. We analyzed the structural characteristics of the bacterial and fungal communities in both rhizosphere and bulk soil samples. Upon thorough analysis, BF and MS were determined to be most effective tree species for urban forest ecosystems. Mantel analysis revealed that Electrical Conductivity (EC), Available potassium (AK), and Microbial Biomass Carbon (MBC) are the primary soil factors affecting rhizosphere microorganisms, while Total Nitrogen (TN) and AK predominantly affected bulk soil microorganisms. Finally, through a random forest model and a Structural Equation Modeling (SEM), it revealed that soil physicochemical properties, rather than microbial biomass and soil enzymatic activity, are the dominant factors influencing seasonal variations in soil microbial communities.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"209 ","pages":"Article 105995"},"PeriodicalIF":4.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sand dune fixation enhances the contribution of microbial necromass carbon to soil organic carbon: A case study of Mu Us Sandy Land in China","authors":"Qing Qu ,&nbsp;Zhen Wang ,&nbsp;Hongwei Xu ,&nbsp;Rentao Liu ,&nbsp;Minggang Wang ,&nbsp;Sha Xue","doi":"10.1016/j.apsoil.2025.106011","DOIUrl":"10.1016/j.apsoil.2025.106011","url":null,"abstract":"<div><div>Restoration of vegetation, soil, and biodiversity is key crucial for managing desertified ecosystems; however, whether desertification restoration promotes the accumulation of microbial necromass carbon (NC) remains unclear. In this study, four dune types (mobile, semi-mobile, semi-fixed, and fixed) were selected to represent different desertification restoration stages, and the accumulation mechanism of microbial NC and its contribution to soil organic carbon (SOC) were analyzed. The results showed that since the plant diversity, soil water content, and microbial activity of semi-mobile, semi-fixed, and fixed dunes were higher than those of mobile dunes, the SOC in semi-mobile (2.63 g kg⁻¹), semi-fixed (2.98 g kg⁻¹), and fixed (3.82 g kg⁻¹) dunes were significantly higher than that in mobile dune (0.98 g kg⁻¹). Moreover, sand dune fixation promoted microbial NC accumulation and increased microbial NC/SOC ratio but decreased fungal NC/bacterial NC ratio. In addition, our study showed that plant diversity is the main factor influencing microbial NC, which has direct and indirect effects on microbial NC accumulation. In conclusion, sand dune fixation enhances microbial NC accumulation and its contribution to SOC, and the change is driven mainly by plant diversity. This study enhances our understanding of the microbial NC accumulation mechanism during dune restoration.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"209 ","pages":"Article 106011"},"PeriodicalIF":4.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shrub effect in semiarid Monte rangelands: Variation of arbuscular mycorrhizal fungi communities associated with different functional groups of plants","authors":"Mariela Lis Ambrosino ,&nbsp;Yanina Alejandra Torres ,&nbsp;Antonio Francisco Garayalde ,&nbsp;Lorena Vanesa Armando ,&nbsp;Graciela Susana Lorda ,&nbsp;María Silvana Velázquez","doi":"10.1016/j.apsoil.2025.106000","DOIUrl":"10.1016/j.apsoil.2025.106000","url":null,"abstract":"<div><div>The presence of shrubs in semiarid rangelands has been associated with changes in the spatial distribution of soil resources. However, the relationships between woody species and arbuscular mycorrhizal fungi (AMF) remain unclear. This study investigated the influence of shrub cover on AMF communities and their associations with soil quality indicators. In spring 2017, five paddocks were selected in a semiarid Monte rangeland of Argentina. Two 10-m transects were established in each paddock: one in a shrub-dominated patch (Sh) and one in a shrub-free patch (WSh). Within each transect, sampling sites were categorized as grass-covered (Sh-G and WSh-G), bare ground-litter (Sh-BL and WSh-BL), or under shrubs (Sh-S). Composite soil samples (5 cores per site, 0–10 cm in depth) were collected within each site for AMF spore extraction and identification. Entrophosporaceae abundance was higher in Sh-BL than Sh-G and Sh-S sites. Glomeraceae abundance was lower in Sh-G and Sh-BL than in the same sites in WSh transects. Ruderal/rhizophilic AMF presented higher abundance in WSh-BL than in Sh-S and Sh-BL sites, while stress tolerant/ancestral AMF were more abundant in Sh-G than in WSh-G sites. Soil organic matter, its labile fraction, and cellulase activity correlated positively with the abundance of Ambisporaceae and Pacisporaceae, but negatively with Entrophosporaceae. These results indicate that shrub species tend to favor the presence of stress tolerant/ancestral AMF over more generalist or ruderal/rhizophilic types commonly found in the soil nearby. These effects may be driven by increased organic fractions and microbial activity associated with the soil carbon cycle.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"209 ","pages":"Article 106000"},"PeriodicalIF":4.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term plastic film mulching promotes microplastic accumulation and alters gross nitrogen transformation in soil","authors":"Jinrui Zhang ,&nbsp;Kai Wang ,&nbsp;Tianxiang Hao ,&nbsp;Jinbo Zhang ,&nbsp;Christoph Müller ,&nbsp;Perrine Florent ,&nbsp;Hong Yan ,&nbsp;Siyang Ren ,&nbsp;Kaijing Qu ,&nbsp;Kaige Ren ,&nbsp;Jingjing Li ,&nbsp;Yiting Su ,&nbsp;Fan Ding ,&nbsp;Jingkuan Wang ,&nbsp;Xihe Wang ,&nbsp;Yanling Chen ,&nbsp;Shihua Lv ,&nbsp;David R. Chadwick ,&nbsp;Davey L. Jones ,&nbsp;Xuejun Liu","doi":"10.1016/j.apsoil.2025.106007","DOIUrl":"10.1016/j.apsoil.2025.106007","url":null,"abstract":"<div><div>While long-term plastic film mulching (LFM) of farmland can improve the yield and quality of crops, it also poses ecological risks through the accumulation of microplastics (MPs) in soil and alterations in soil nitrogen (N) cycling. However, no systematic studies (based on long-term experiments) have studied the effects of LFM on both soil MPs accumulation and gross N transformations. In this study, topsoils (0–20 cm) were collected from four LFM farmlands in Xinjiang, Liaoning, Sichuan, and Shandong provinces of China. The ¹⁵N isotope pool dilution method and <em>Ntrace</em>_{<em>basic</em>} model were applied to quantify the impact of LFM on soil gross N transformation rates. Our results showed that LFM significantly increased the accumulation of MPs, particularly in the 0–10 cm layer. The gross N transformation rates varied among sites, reflecting regional differences in soil type. Mineralization rates increased in Xinjiang, Liaoning, and Sichuan under LFM, while microbial assimilation and autotrophic nitrification decreased in Xinjiang. In Sichuan, reduced soil nitrification potential led to low levels of mineral N (NH₄⁺-N and NO₃⁻-N) retention. Furthermore, in Shandong, LFM decreased the mineralization potential of recalcitrant organic N but significantly enhanced heterotrophic nitrification. To improve predictions of agroecosystem N cycling, we show with this study that it is important to consider soil differences which drive gross N transformation rates associated with LFM.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106007"},"PeriodicalIF":4.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of plant functional group and reclamation treatments on microbial networks and nutrient limitation in initial soil developed on spoil heaps after hard coal mining","authors":"Wojciech Bierza ,&nbsp;Artur Trzebny ,&nbsp;Agnieszka Kompała-Bąba ,&nbsp;Bartłomiej Woś ,&nbsp;Marcin Pietrzykowski ,&nbsp;Agnieszka Józefowska ,&nbsp;Marek Pająk ,&nbsp;Krzysztof Otremba ,&nbsp;Edyta Sierka","doi":"10.1016/j.apsoil.2025.106002","DOIUrl":"10.1016/j.apsoil.2025.106002","url":null,"abstract":"<div><div>Plant functional groups (PFGs), together with the type of reclamation treatment applied, can influence the changes in soil nutrient availability. The stoichiometric imbalance between resources can alter the activity and structure of microbial communities which play a crucial role in regulating carbon and nutrient cycling in initial soil ecosystems. However, the response of microbial networks within the soil food web in spoil heaps to stoichiometric imbalances has not yet been investigated. In this study, we examine how soil microbial networks webs respond to elemental limitation in unreclaimed sites on carboniferous bare rock and reclaimed with topsoiling in the presence of two PFGs, i.e. grasses and forbs, which are commonly used in the spoil heap reclamation processes. To understand these relationships, soil physicochemical properties, enzyme stoichiometry, abundances and community compositions of soil bacteria and fungi were investigated. We found that the availability of P most limited the formation of soil microbial networks. Both types of PFGs stimulated the formation of complex soil microbial networks but didn't affect the nutrient limitation of the soil microbial community differently. Soil microbiome in bare rock was P-limited and characterized by disturbed structure and lower diversity. Topsoiling increased biodiversity and improved the structure of soil microbial communities. The application of topsoiling showed no positive effect on the stability and complexity of the soil microbial networks. Our findings indicate that P-limitation needs to be considered in understanding and predicting biogeochemical cycles on unreclaimed heaps where spontaneous succession occurs. Topsoiling appears to be a good way to reclaim heaps with an adequate nitrogen supply to the soil.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106002"},"PeriodicalIF":4.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil microbiome community composition shaped by soil depth in a wetland with diel variations in methane emissions","authors":"Stacey M. Trevathan-Tackett ,&nbsp;Luke C. Jeffrey ,&nbsp;Anne Yusuf ,&nbsp;Paul E. Carnell ,&nbsp;Damien T. Maher ,&nbsp;Scott G. Johnston ,&nbsp;Peter I. Macreadie","doi":"10.1016/j.apsoil.2025.106005","DOIUrl":"10.1016/j.apsoil.2025.106005","url":null,"abstract":"<div><div>Wetlands play a disproportionally important role in the global methane cycle due to their unique hydrological and biogeochemical characteristics. Understanding the complex interplay among microbial communities, habitat and geochemical processes is key for assessing their response to environmental changes and their contribution to greenhouse gas dynamics. This study investigated the spatiotemporal and depth relationships among methane fluxes, soil geochemistry, and microbiome communities in a subtropical wetland using 16S rRNA sequencing, methane flux measurements, and soil profiling. We find that soil chemical properties and methane are linked to the variations in soil microbial communities. However, soil depth is the primary factor structuring microbial communities, with surface soils supporting high abundance of iron-methane cycling microbes and evidence of direct interspecies electron transfer (DIET) pathways. Interconnected processes involving methanogens, syntrophs, sulphur reducing bacteria, and fermentative bacteria were prominent in surface soils, likely facilitating organic matter decomposition and methane production. Variations in diurnal methane dynamics and water chemistry were linked to shifts in the relative abundance of microbial taxa, such as Methylomirabilaceae, <em>Syntrophobacter,</em> and <em>Syntrophorhabdus</em>. Water lilies (<em>Nymphaea</em> sp.) are possibly influencing microbial activity and methane emissions in wetlands by supplying organic matter and oxygen to the soil. Overall, our results show that soils depth drove microbial community, with abiotic (e.g. temperature) and biotic factors (e.g. vegetation) influencing spatiotemporal variation in wetland methane fluxes. Understanding the complex drivers of methanogenesis in wetlands is essential for refining global methane budgets and accurately modelling future climate scenarios in the face of accelerating environmental change.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106005"},"PeriodicalIF":4.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grazing exclusion changes the complexity and stability but not the α-diversity of the microbial community in a desert steppe","authors":"Fengqin Yuan ,&nbsp;Xuebao Xu ,&nbsp;Zhenpeng Liu ,&nbsp;Rula Sa ,&nbsp;Chongzhi Sun ,&nbsp;Jia Liu ,&nbsp;Na Li ,&nbsp;Yujuan Zhang ,&nbsp;Tongrui Zhang ,&nbsp;Tingting Xing ,&nbsp;Jie Ren ,&nbsp;Shiming Tang ,&nbsp;Ke Jin","doi":"10.1016/j.apsoil.2025.106003","DOIUrl":"10.1016/j.apsoil.2025.106003","url":null,"abstract":"<div><div>Grazing exclusion (GE), to achieve ecological restoration, has a significant impact on plant community features and soil physicochemical qualities, including soil fertility. Whereas, the responses of soil microbial diversity, complexity, and stability to GE in a water-limited desert steppe remain poorly understood. GE of different durations (14 and 20 years) was implemented in a desert steppe of central Inner Mongolia in this study. Changes in soil physicochemical properties and chemical stoichiometry were observed with increasing restoration time, accompanied by variations in the composition of the microbial community. GE did not alter α-diversity, but it did change the microbial community structure and composition, with significant differences between those in the topsoil and subsoil. A symbiotic network analysis revealed a gradual increase in the complexity of soil bacterial and fungal networks with the increasing GE duration, and that the rate of change was greater in the bacterial than in the fungal community, with greater changes in the latter in the subsoil than in the topsoil. There was no significant change in the stability of the bacterial network under GE whereas the stability of the surface fungal network increased and then decreased, and the underlying stability increased significantly. The Zi-Pi plots and CCA (Canonical Correlation Analysis) analysis revealed important roles for the bacterial phyla Actinobacteriota, Proteobacteria, and Chloroflexi as well as for the fungal phyla Ascomycota, Mortierellomycota, and Basidiomycota in the response of the soil to environmental variations. Available nitrogen and the carbon to nitrogen ratio were the environmental determinants to affect the structure of topsoil fungal community (<em>P</em> &lt; 0.05). As for the subsoil, available phosphorus and above-ground biomass were the environmental determinants to affect its structure (<em>P</em> &lt; 0.05; <em>P</em> &lt; 0.01). Our findings highlighted the critical role of ecosystem restoration duration in shaping soil microbial community in water-stressed steppes. Specifically, the duration significantly influenced the complexity and stability of these community. Furthermore, the community exhibited differential adaptive responses to environmental changes.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106003"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of cropping alfalfa (Medicago sativa L.) on the soil pore structure, microbial communities and organic carbon fractions in saline soils","authors":"Dan Zhu ,&nbsp;Lei Sun ,&nbsp;Lina Mao ,&nbsp;Jingyang Li ,&nbsp;Bohan Yan ,&nbsp;Bin Li ,&nbsp;Xin Li","doi":"10.1016/j.apsoil.2025.105993","DOIUrl":"10.1016/j.apsoil.2025.105993","url":null,"abstract":"<div><div>Soil salinity is a global environmental issue that poses a serious threat to soil quality harming the terrestrial ecosystems, and the Songnen Plain in Northeast China exemplifies saline-alkaline soils. Alfalfa has played an important role in the improvement of saline and alkaline land in the Songnen Plain, which has significantly increased the agricultural production potential of saline and alkaline land. However, limited studies have investigated the specific effects of alfalfa on soil aggregate pore structure, microbial community composition, and soil carbon content, which are critical factors in soil restoration. Accordingly, using high-throughput sequencing and computed tomography (CT) to examine how alfalfa cropping impacts these key soil properties, specifically, soil aggregate porosity, microbial diversity, and soil organic carbon content. The findings revealed that cropping alfalfa significantly increased the porosity of the topsoil, especially the increase in macropores (P_{&gt; 100 μm}) was more pronounced. Topsoil organic carbon content and its carbon fractions were significantly increased, with soil organic carbon (SOC), mineral-associated organic carbon (MAOC), particulate organic carbon (POC), readily oxidizable carbon (ROC), and dissolved organic carbon (DOC) were all increased. Alfalfa cropping also enhanced microbial community structure, as evidenced by larger and more interconnected microbial co-occurrence networks. In the topsoil, the number of nodes increased by 787 and 803, while the number of edges grew by 1067 and 8186 in bacterial and fungal networks, respectively. Similarly, in the subsoil, bacterial and fungal networks exhibited increases of 1819 and 289 nodes, and 11,422 and 2372 edges, respectively. These complex networks demonstrated greater resilience to saline-alkaline stress, with keystone microbial species influenced by organic carbon fractions driving community assembly primarily through stochastic processes. The study also highlighted significant vertical spatial variability, with alfalfa cropping having a more pronounced effect on topsoil compared to subsoil. In addition, cropping alfalfa improved the physical and chemical properties of saline soils, enhanced soil aggregate stability, increased macropore proportions, boosted microbial diversity, and contributed to the ecological restoration of saline soils. These findings provide valuable insights into the mechanisms by which alfalfa improves saline soils and offer a promising approach for the restoration and sustainable management of saline-alkaline ecosystems.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 105993"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gypsum application increases microbial activity and organic carbon mineralization in saline paddy soils","authors":"Yixian Liu ,&nbsp;Ze Zhang ,&nbsp;Xiangxiang Wang ,&nbsp;Ruiqiao Wu ,&nbsp;Shuai Ding ,&nbsp;Shuang Wang ,&nbsp;Jianping Chen ,&nbsp;Tida Ge ,&nbsp;Zhenke Zhu","doi":"10.1016/j.apsoil.2025.106004","DOIUrl":"10.1016/j.apsoil.2025.106004","url":null,"abstract":"<div><div>Saline–alkaline soils, representing approximately 10 % of global soil resources, are characterized by high salinity and pH levels, low microbial activity, and inefficient soil organic carbon (SOC) accumulation. Gypsum application is a common practice for ameliorating saline soils, as it reduces exchangeable sodium and improves soil physicochemical properties. However, the impact of gypsum on soil microbial activity and organic carbon turnover remains insufficiently understood. Therefore, we conducted incubation experiments to investigate the effects of gypsum application on microbial community composition and OC mineralization in soils with varying salinity levels (154, 268, 646, and 865 μS cm⁻¹). Gypsum application significantly increased the mineralization of both glucose (exogenous organic carbon (EOC)) and SOC compared to the control (no gypsum), particularly in high-salinity soils, with increases of 282 % and 249 %, respectively. This enhancement was attributed to a 50 % reduced exchangeable sodium, which alleviated microbial salt stress and shifted microbial life strategies. Gypsum application also increased microbial abundance and decreased microbial diversity, favoring taxa growth that adapted to the reduced exchangeable sodium condition. Microbial network analysis revealed a 17.19 % increase in network edges and a 63-edge increase in high-salinity soils following gypsum application. Structural equation modeling indicated that improvements in environmental factors (e.g., soil ionic composition, +0.10) and microbial activity (+0.51) both contributed to the enhanced EOC mineralization after gypsum application. Overall, our findings suggest that gypsum application ameliorates high-salinity soils by boosting microbial activity and accelerating carbon utilization and mineralization, which holds significant implications for promoting EOC metabolism and SOC accumulation in saline soils.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106004"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic fertilizations alter the abundance and diversity of soil microbial genes involved in C, N, P mineralization in a coastal poplar plantation","authors":"Tingting Ren ,&nbsp;Jiahui Liao ,&nbsp;Xiaoming Zou ,&nbsp;Yuanyuan Li ,&nbsp;Juanping Ni ,&nbsp;Ke Shi ,&nbsp;Long Jin ,&nbsp;Manuel Delgado-Baquerizo ,&nbsp;Honghua Ruan","doi":"10.1016/j.apsoil.2025.106001","DOIUrl":"10.1016/j.apsoil.2025.106001","url":null,"abstract":"<div><div>Organic fertilization supports the sustainability of managed ecosystems; however, investigations into how microbial-driven mineralization processes of carbon (C), nitrogen (N), and phosphorus (P) interact with soil functions under the application of organic fertilizers remain limited. We investigated the impacts of six years of applying biogas-slurry and biochar on the abundance and diversity of soil microbial genes involved in C, N, and P mineralization in a poplar plantation. Our findings indicated that the addition of biogas-slurry alone, as well as in combination with biochar, reduced the diversity of microbial genes involved in C, N, and P mineralization. The addition of biogas-slurry increased the abundance of these genes, but the application of biochar decreased it. Using thresholds from the eco-enzyme vector model, we found that the addition of biogas-slurry, either alone or in combination with biochar, alleviated microbial P limitation by decreasing the N:P ratio of microbial biomass and increasing soil dissolved organic C (DOC). Additionally, linear regression indicated that the alleviation of microbial P limitation suppressed the diversity of genes and promoted the abundance of genes involved in C mineralization. Random forest and partial dependence analyses showed that increased DOC was the major factor responsible for the decreased diversity of microbial genes and increased abundance of genes involved in N mineralization. The SOC: TN ratio was negatively correlated with the abundance of genes involved in N and P mineralization. These findings highlight the inconsistent responses of the abundance and diversity of microbial genes involved in C, N, and P mineralization to the application of organic fertilizers in managed ecosystems. Additionally, these varying responses are regulated by increasing nutrient supplies and alleviating microbial P limitation. Our findings provide a new understanding of soil carbon and nutrient cycling and suggest the application of organic fertilizers to facilitate the sustainable management of ecosystems in the future.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"208 ","pages":"Article 106001"},"PeriodicalIF":4.8,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}