Annals of The Entomological Society of America最新文献

{"title":"Limited Mating Ability of a Wasp Strain with Rickettsia-Induced Thelytoky","authors":"T. Adachi-Hagimori, K. Miura","doi":"10.1093/aesa/saaa007","DOIUrl":"https://doi.org/10.1093/aesa/saaa007","url":null,"abstract":"Abstract Sexual reproduction is the dominant mode of reproduction in plants and animals; however, some species from various taxonomic groups reproduce asexually. Because some of these asexual species lack DNA recombination and so have low genetic variability, these asexual species are more likely to go extinct than sexual species. Neochrysocharis formosa (Westwood) (Hymenoptera: Eulophidae) is a solitary endoparasitoid in which both arrhenotokous and thelytokous strains occur in sympatry. The thelytokous strain is infected by a parthenogenesis-inducing Rickettsia bacterium. We investigated whether fertilized progeny can be produced between females and antibiotic-induced males of the thelytokous strain. The males produced by antibiotic treatment showed the same courtship behaviors as the arrhenotokous males, but at a lower rate, and did not produce fertilized progeny. The results confirm that the thelytokous strain has been maintained by a functional apomixis mechanism rather than by occasional sex, preserving a degree of heterozygosity.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"355 - 358"},"PeriodicalIF":2.3,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saaa007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44988868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Interspecific Chemical Competition Between Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) Reduces Fecundity and Hastens Development Time”","authors":"M. Bullock, Geoffrey Legault","doi":"10.1093/aesa/saaa006","DOIUrl":"https://doi.org/10.1093/aesa/saaa006","url":null,"abstract":"The flour beetles Tribolium castaneum (Herbst) and Tribolium confusum (du Val) are model organisms for studying the effects of intraand interspecific competition. Both species are known to compete directly through egg cannibalism and indirectly through the density-dependent release of allelopathic chemicals. To better characterize these indirect interactions, recent work has focused on quantifying the per-capita effects of intraspecific chemical competition. However, the effects of interspecific chemical competition in this system have not previously been estimated. We used experimental microcosms to examine how interspecific chemical secretions affected the reproductive activity and development time of laboratory populations of T. castaneum and T. confusum. We created replicated habitats containing flour medium that had been occupied and chemically ‘conditioned’ by one of the two beetle species across a range of densities, then examined how beetles of the other species responded in terms of the number of eggs laid by females and, separately, the development time of offspring. We found that T. castaneum fecundity was reduced when beetles experienced flour conditioned by T. confusum. In contrast, fecundity of T. confusum was largely unaffected by flour conditioned by T. castaneum. Additionally, we found that interspecific conditioning decreased development times for beetles of both species, particularly the development of larvae to pupae. Our results indicate that interspecific chemical competition impacts the life history of Tribolium species and suggests that models incorporating chemical competition may more accurately describe the biology of flour beetle communities.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"223 - 223"},"PeriodicalIF":2.3,"publicationDate":"2020-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saaa006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46053833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Diagnostic Genetic Markers for a Cryptic Invasive Agricultural Pest: A Test Case Using the Apple Maggot Fly (Diptera: Tephritidae)","authors":"Meredith M. Doellman, G. Hood, Jacob Gersfeld, Amanda L. Driscoe, Charles C. Y. Xu, Ryan N. Sheehy, Noah Holmes, W. Yee, J. Feder","doi":"10.1093/aesa/saz069","DOIUrl":"https://doi.org/10.1093/aesa/saz069","url":null,"abstract":"Abstract Insect pests destroy ∼15% of all U.S. crops, resulting in losses of $15 billion annually. Thus, developing cheap, quick, and reliable methods for detecting harmful species is critical to curtail insect damage and lessen economic impact. The apple maggot fly, Rhagoletis pomonella, is a major invasive pest threatening the multibillion-dollar apple industry in the Pacific Northwest United States. The fly is also sympatric with a benign but morphologically similar and genetically closely related species, R. zephyria, which attacks noncommercial snowberry. Unambiguous species identification is essential due to a zero-infestation policy of apple maggot for fruit export. Mistaking R. zephyria for R. pomonella triggers unnecessary and costly quarantines, diverting valuable control resources. Here we develop and apply a relatively simple and cost-effective diagnostic approach using Illumina sequencing of double-digest restriction site-associated DNA markers. We identified five informative single-nucleotide polymorphisms (SNPs) and designed a diagnostic test based on agarose gel electrophoresis of restriction enzyme-digested polymerase chain reaction amplification products (RFLPs) to distinguish fly species. We demonstrated the utility of this approach for immediate, 1-d species identification by scoring apple- and snowberry-infesting flies from known hosts, reared from fruit collected at 11 sites throughout Washington. However, if immediate diagnosis is not required, or hundreds to thousands of specimens must be assessed, then a direct Illumina-based sequencing strategy, similar to that used here for diagnostic SNP identification, can be powerful and cost-effective. The genomic strategy we present is effective for R. pomonella and also transferable to many cryptic pests.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"246 - 256"},"PeriodicalIF":2.3,"publicationDate":"2020-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saz069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46393528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of a Special Issue on Advanced Genetic Analysis of Invasive Arthropod Species","authors":"J. Morisette, S. Burgiel, Keith D. Gaddis","doi":"10.1093/aesa/saaa005","DOIUrl":"https://doi.org/10.1093/aesa/saaa005","url":null,"abstract":"1National Invasive Species Council (NISC) Staff, US Department of the Interior, Office of the Secretary, c/o National Wildlife Research Center, 4101 La Porte Avenue, Fort Collins, CO 80521, 2National Invasive Species Council (NISC) Staff, US Department of the Interior, Office of the Secretary, 1849 C Street NW, Mailstop 2061, Washington, DC 20240, 3National Aeronautics and Space Administration, NASA Headquarters, Earth Science Division, 300 E Street Southwest, Washington, DC 20546, and 4Corresponding author, e-mail: jeffrey_morisette@ios.doi.gov","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"225 - 229"},"PeriodicalIF":2.3,"publicationDate":"2020-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saaa005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45805707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Genes That Result in High Mortality of Oryctes rhinoceros (Scarabaeidae: Coleoptera) When Targeted Using an RNA Interference Approach: Implications for Large Invasive Insects","authors":"Shizu Watanabe, Brandi-Leigh Adams, A. Kong, Nelson Masang, Tomie S. Vowell, M. Melzer","doi":"10.1093/aesa/saz057","DOIUrl":"https://doi.org/10.1093/aesa/saz057","url":null,"abstract":"Abstract Coconut rhinoceros beetle, Oryctes rhinoceros (L., 1758), is a large scarab beetle native to Southeast Asia and a major pest of coconut (Cocos nucifera) and oil (Elaeis guineensis) palms in its invaded range. Few tools are available for coconut rhinoceros beetle management, particularly for an emerging haplotype with resistance to known strains of Oryctes rhinoceros nudivirus, the traditional biological control agent used in coconut rhinoceros beetle management programs. RNA interference (RNAi) represents an emerging tool for insect pest control that exploits an existing pathway for gene regulation in the target organism. In this study, we evaluated RNAi as a potential tool for coconut rhinoceros beetle management. Using transcriptome data generated from gut tissue of early instar larvae, we identified 24 RNAi target sequences that were either highly expressed or had demonstrated efficacy in other insect systems. Double-stranded (ds)RNAs ranging from 249 to 297 bp in length were generated for 23 of these target sequences and 150 ng were microinjected into coconut rhinoceros beetle 1st, 2nd, and 3rd instar larvae and adults. Five of these dsRNAs that targeted genes putatively encoding V-type ATPase, polyadenylate binding protein, and three forms of actin induced 30.8–100% mortality within 14 days post injection (dpi). Microinjection of 2nd instars with 10 and 100 ng of these same five dsRNAs induced 20–100% and 80–100% mortality at 7 and 14 dpi, respectively. These results indicate RNAi should be explored as a possible management option for coconut rhinoceros beetle. Coconut rhinoceros beetle may also represent a model species for using RNAi in the management of large invasive insect species.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"310 - 317"},"PeriodicalIF":2.3,"publicationDate":"2020-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saz057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42660991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementing Low-Cost, High Accuracy DNA Barcoding From Single Molecule Sequencing to Screen Larval Tephritid Fruit Flies Intercepted at Ports of Entry","authors":"Ivonne J. Garzón-Orduña, S. Geib, Lisa Ledezma, Forest T. Bremer, N. Barr","doi":"10.1093/aesa/saz071","DOIUrl":"https://doi.org/10.1093/aesa/saz071","url":null,"abstract":"Abstract Exotic fruit flies in the family Tephritidae pose a threat to U.S. agriculture and natural resources. As part of ongoing fruit fly detection and exclusion programs, invasive fruit fly adults are periodically trapped in and introduced populations are eradicated from two major fruit producing states: California and Florida. Although the pathways used by flies to enter these states are not clear, one possible introduction pathway is hand-carrying fruits and vegetables infested with eggs or larvae through ports of entry during border crossings or international air travel. These collections represent an important resource for determining what species are most commonly associated with particular ports of entry, their host plants, and source country, making the identification of the intercepted larvae fundamental. Here, we examine cytochrome c oxidase subunit I sequences obtained with Sanger DNA sequencing and Single Molecule Real-time sequencing (SMRT) technologies on a collection of hundreds of intercepted larvae and pupae that are suspected to be fruit pests found in the subfamily Dacinae, in addition to a collection of field-caught flies. Utilizing Pacific Biosciences (PacBio) Circular Consensus Sequencing, we sequenced thousands of independent amplicons in a single SMRT cell. We present our implementation of PacBio's method by describing wet-lab procedures as well as analytical protocols to automate the process. Finally, for the intercepted material, we compared the generated SMRT data to sequences produced with Sanger and evaluate their quality as a means to identify flies. Using intercepted material, we obtained a sequencing success of over 88% with PacBio's SMRT sequencing. As the spread of invasive insects grow, so will the size of collections of intercepted material; we found technologies such as SMRT sequencing to be excellent resources to simplify the generation of large amounts of molecular data and to reduce or eliminate all together the manipulation of the sequences.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"288 - 297"},"PeriodicalIF":2.3,"publicationDate":"2020-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saz071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45078282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographic Information System (GIS)-Based Mapping and Spatial Analyses Applied to Risk Assessment and Resource Allocation for Boll Weevil (Coleoptera: Curculionidae) Detection","authors":"M. Brewer, Leonel Deleon, Isaac L. Esquivel","doi":"10.1093/aesa/saz048","DOIUrl":"https://doi.org/10.1093/aesa/saz048","url":null,"abstract":"Abstract Boll weevil, Anthonomous grandis grandis Boheman first invaded U.S. cotton in Texas in the late 1800s, and spread throughout U.S. cotton-growing regions by the 1920s. Boll weevil eradication efforts initiated in the eastern United States in the 1980s resulted in its elimination except in the southernmost region of Texas and adjoining areas of Mexico. We focused on geographic information system (GIS)-based mapping and spatial analyses of boll weevil trap data to consider whether landscape features were associated with spatially variable detections of boll weevils. Seven years of trap data were overlaid with data layers of vector-based classifications of cropland, transportation, and hydrological features. New boll weevil detections in 2018 were 108 km north of cotton fields in the Lower Rio Grande Valley (LRGV) where more persistent detections occurred. Focusing on LRGV data, 14 of 24 correlations of number of boll weevil captures to nearest distances to selected landscape features were negative. In follow-up analyses, best data fit was seen using stepwise regression. In 2010, waterbodies and watermelon fields were influential linear terms (partial R2 = 0.14 and 0.064, respectively; model R2 = 0.32). In 2014, the Rio Grande River as a linear term was influential (partial R2 = 0.15; model R2 = 0.24). Boll weevil captures tended to increase in closer proximity to these landscape features. Results of 2010 were consistent with expectations of remnant populations in the LRGV spreading locally, while 2014 results may reflect remnant populations or re-introductions from boll weevil moving longer distances into the LRGV.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"71 - 78"},"PeriodicalIF":2.3,"publicationDate":"2020-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saz048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45159006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of a Special Issue on Geospatial Analysis of Invasive Arthropod Alien Species","authors":"J. Morisette, K. Macaluso, S. Burgiel","doi":"10.1093/aesa/saaa002","DOIUrl":"https://doi.org/10.1093/aesa/saaa002","url":null,"abstract":"1National Invasive Species Council (NISC) Staff, US Department of the Interior, Office of the Secretary, c/o National Wildlife Research Center, 4101 La Porte Avenue, Fort Collins, CO 80521, 2Department of Microbiology & Immunology MSB – 5851, University of South Alabama, Dr. N, Room 2102LID 610 Clinic Drive, Mobile, AL 36688, 3National Invasive Species Council (NISC) Staff, US Department of the Interior, Office of the Secretary, 1849 C Street, N.W., Mailstop 3530, Washington, DC 20240, and 4Corresponding author, e-mail: jeffrey_morisette@ios.doi.gov","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"67 - 70"},"PeriodicalIF":2.3,"publicationDate":"2020-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saaa002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45472156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interspecific Chemical Competition Between Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) Reduces Fecundity and Hastens Development Time","authors":"M. Bullock, Geoffrey Legault, B. Melbourne","doi":"10.1093/aesa/saaa001","DOIUrl":"https://doi.org/10.1093/aesa/saaa001","url":null,"abstract":"Abstract The flour beetles Tribolium castaneum (Herbst) and Tribolium confusum (du Val) are model organisms for studying the effects of intra- and interspecific competition. Both species are known to compete directly through egg cannibalism and indirectly through the density-dependent release of allelopathic chemicals.To better characterize these indirect interactions, recent work has focused on quantifying the per-capita effects of intraspecific chemical competition. However, the effects of interspecific chemical competition in this system have not previously been estimated. We used experimental microcosms to examine how interspecific chemical secretions affected the reproductive activity and development time of laboratory populations of T. castaneum and T. confusum. We created replicated habitats containing flour medium that had been occupied and chemically ‘conditioned’ by one of the two beetle species across a range of densities, then examined how beetles of the other species responded in terms of the number of eggs laid by females and, separately, the development time of offspring. We found that T. castaneum fecundity was reduced when beetles experienced flour conditioned by T. confusum. In contrast, fecundity of T. confusum was largely unaffected by flour conditioned by T. castaneum. Additionally, we found that interspecific conditioning decreased development times for beetles of both species, particularly the development of larvae to pupae. Our results indicate that interspecific chemical competition impacts the life history of Tribolium species and suggests that models incorporating chemical competition may more accurately describe the biology of flour beetle communities.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"113 1","pages":"216 - 222"},"PeriodicalIF":2.3,"publicationDate":"2020-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saaa001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45142491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistence of Two Salmonella enterica ser. Montevideo Strains Throughout Horn Fly (Diptera: Muscidae) Larval and Pupal Development","authors":"P. Olafson, K. B. Temeyer, K. Lohmeyer, T. Edrington, G. Loneragan","doi":"10.1093/aesa/saw085","DOIUrl":"https://doi.org/10.1093/aesa/saw085","url":null,"abstract":"Abstract Strains of Salmonella enterica can be subdivided into clades that differ in their genetic composition, influencing microbial ecology and bacterial transmission. Salmonella serovar Montevideo strains 1110 and 304, representatives of two different clades, were used to evaluate interactions with the various stages of horn fly development. Sterilized cattle dung was inoculated with Salmonella monocultures, and horn fly larvae were exposed to 103, 105, and 107 colony-forming units (CFU)/g per strain. Salmonella supported horn fly development, and concentration-dependent differences in pupal survival suggested that Salmonella Montevideo 304 impacts adult emergence when larvae are reared in a high concentration. Viable bacteria of each strain were quantified from larvae, pupae, and newly emerged adults. Both strains were cultured from larvae at a mean ~105, regardless of concentration, and both strains survived pupation. Quantities of Salmonella 1110 were stationary through the midpupal stage, after which quantities declined in pupae reared in 105 and increased twofold in pupae reared in 107 CFU/g. Quantities of Salmonella 304 remained stationary throughout pupal development when reared in 105, yet increased 29-fold when reared in 107 CFU/g. At high densities, properties of Salmonella 304 may influence its interaction with horn fly larvae, enabling the bacteria to evade degradation during larval gut histolysis and to subsequently proliferate during the late stages of pupal development. This may account for the observed effect on adult emergence. The Salmonella strains were rarely cultured from newly emerged adults, indicating that transstadial carriage to the adult stage is inefficient.","PeriodicalId":8076,"journal":{"name":"Annals of The Entomological Society of America","volume":"110 1","pages":"54 - 60"},"PeriodicalIF":2.3,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/aesa/saw085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46051063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}