Methods in cell science : an official journal of the Society for In Vitro Biology最新文献_第2页

Coulter counter use in the enumeration of muscle and fat stem cells. 库尔特计数器在肌肉和脂肪干细胞计数中的应用。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1007/s11022-004-2382-5

Melinda E Fernyhough, Deri L Helterline, Janet L Vierck, Rod A Hill, Michael V Dodson

引用次数: 8

Zebrafish retinal slice preparation. 斑马鱼视网膜切片制备。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1023/B:MICS.0000006853.54435.85

V P Connaughton

引用次数: 8

Zebrafish as an experimental model: strategies for developmental and molecular neurobiology studies. 斑马鱼作为实验模型:发育和分子神经生物学研究的策略。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1023/B:MICS.0000006849.98007.03

Brian Key, Christine A Devine

{"title":"Zebrafish as an experimental model: strategies for developmental and molecular neurobiology studies.","authors":"Brian Key, Christine A Devine","doi":"10.1023/B:MICS.0000006849.98007.03","DOIUrl":"https://doi.org/10.1023/B:MICS.0000006849.98007.03","url":null,"abstract":"Zebrafish provide a rapid and effective means for assessing gene function in the vertebrate nervous system. By employing gain- and loss-of-function techniques it is possible to obtain insights into the roles of both wild-type and heterologously expressed genes. Such approaches enable rapid progression from gene discovery to gene expression and finally to gene function even when examining development of a tissue as complex as the nervous system. Exploiting the full potential of zebrafish as a bioassay for the nervous system will require, not only an understanding of the molecular and cellular basis of normal zebrafish development, but also an appreciation of comparative processes in other species. When applied to mutant animals, classic morphological approaches and contemporary molecular genetic techniques are providing a wealth of information on the development of the nervous system at the molecular, cell, system and behavioural levels. Zebrafish are now emerging as an important tool, supporting mouse genetical approaches for understanding neural function in vertebrates.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"25 1-2","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/B:MICS.0000006849.98007.03","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24177459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

Neural cell fate analysis in zebrafish using olig2 BAC transgenics. 利用olig2 BAC转基因技术分析斑马鱼神经细胞命运。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1023/B:MICS.0000006847.09037.3a

Jimann Shin, Hae-Chul Park, Jolanta M Topczewska, David J Mawdsley, Bruce Appel

引用次数: 264

Assessment of neuronal maturation and acquisition of functional competence in the developing zebrafish olfactory system. 发育中的斑马鱼嗅觉系统中神经元成熟和功能能力获得的评估。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1023/B:MICS.0000006852.16798.34

Yoko Sakata, Jared K Olson, William C Michel

{"title":"Assessment of neuronal maturation and acquisition of functional competence in the developing zebrafish olfactory system.","authors":"Yoko Sakata, Jared K Olson, William C Michel","doi":"10.1023/B:MICS.0000006852.16798.34","DOIUrl":"https://doi.org/10.1023/B:MICS.0000006852.16798.34","url":null,"abstract":"Olfactory coding at the level of the olfactory bulb is thought to depend upon an ensemble response of mitral cells receiving input from chemotopically-organized projections of olfactory sensory neurons and regulated by lateral inhibitory circuits. Immunocytochemical methods are described to metabolically classify neurons in the developing zebrafish olfactory system based on the relative concentrations of taurine, glutamate, GABA (and potentially other small biogenic amines) and a small guanidium-based cation, agmatine, which labels NMDA-sensitive cells by permeating through active ionotropic glutamate receptor channels. Using metabolic profiling in conjunction with activity dependent labeling we demonstrate that neuronal differentiation in the developing olfactory bulb, as assessed by acquisition of a mature neurochemical profile, and sensitivity to an ionotropic glutamate receptor agonist, NMDA, occurs during the second day of development. This experimental approach is likely to be useful in studies concerned with the development of glutamatergic signaling pathways.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"25 1-2","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/B:MICS.0000006852.16798.34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24177904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Analysis of cytosolic and lysosomal pH in apoptotic cells by flow cytometry. 流式细胞术分析凋亡细胞胞浆及溶酶体pH值。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1007/s11022-004-8228-3

Cathrine Nilsson, Katarina Kågedal, Uno Johansson, Karin Ollinger

{"title":"Analysis of cytosolic and lysosomal pH in apoptotic cells by flow cytometry.","authors":"Cathrine Nilsson, Katarina Kågedal, Uno Johansson, Karin Ollinger","doi":"10.1007/s11022-004-8228-3","DOIUrl":"https://doi.org/10.1007/s11022-004-8228-3","url":null,"abstract":"Several reports indicate that the cytosol is acidified during apoptosis although the mechanism is not yet fully elucidated. The most acidic organelle found in the cell is the lysosome, raising the possibility that lysosomal proton release may contribute to the cytosolic acidification. We here describe methods for measurement of the cytosolic and lysosomal pH in U937 cells by a dual-emission ratiometric technique suitable for flow cytometry. Cytosolic pH was analysed in cells loaded with the fluorescent probe BCECF, while lysosomal pH was determined after endocytosis of FITC-dextran. Standard curves were obtained by incubating cells in buffers with different pH in the presence of the proton ionophore nigericin. Apoptosis was induced by exposure of cells to 10 ng/ml TNF-alpha for 4 h, and apoptotic cells were identified using a fluorescent marker for active caspases. By gating of control and apoptotic cells, the cytosolic and lysosomal pH were calculated in each population. The cytosolic pH was found to decrease from 7.2+/-0.1 to 5.8+/-0.1 and the lysosomal increased from 4.3+/-0.4 to 5.2+/-0.3. These methods will be useful in future attempts to evaluate the involvement of lysosomes in the acidification of the cytosol during apoptosis.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"25 3-4","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11022-004-8228-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25203826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 129

Measurement of hemoglobin in long-term fixed erythroid cells: application development for cell science experiments in microgravity. 长期固定红细胞血红蛋白的测定:微重力条件下细胞科学实验的应用进展。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1007/s11022-004-2381-6

Kun Xu, Kerry L Davis, Arthur J Sytkowski

引用次数: 0

Sterile polystyrene culture dishes induce transformation of polyps into medusae in Aurelia aurita (Scyphozoa, Cnidaria). 无菌聚苯乙烯培养皿诱导水螅转化为水母(棘球纲，刺胞纲)。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1007/s11022-004-5592-y

Klaus Herrmann, Barbara Siefker, Stefan Berking

引用次数: 11

Epithelial cell cultures from Botryllus schlosseri palleal buds: accomplishments and challenges. 白葡萄孢嫩芽上皮细胞培养:成就与挑战。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1007/s11022-004-2087-9

Claudette Rabinowitz, Baruch Rinkevich

{"title":"Epithelial cell cultures from Botryllus schlosseri palleal buds: accomplishments and challenges.","authors":"Claudette Rabinowitz, Baruch Rinkevich","doi":"10.1007/s11022-004-2087-9","DOIUrl":"https://doi.org/10.1007/s11022-004-2087-9","url":null,"abstract":"This study focuses on recent improvement in epithelial monolayer cultures originating from whole extirpated Botryllus schlosseri (Urochordata) buds. Buds (n = 2,000) were taken at different ('A' to 'D') blastogenic stages. We tested the suitability of 35 combinations of various substrates and media on attachment, cell spread, epithelial growth frequencies and on monolayer lifespans. Under favorable conditions, cultured buds at blastogenic stages 'B' to 'D' (but not stage 'A') started to attach to the substrates following a 3-day transient period that leads to formation of spheres and attached monolayers. Substrate type is important for the attachment and the development of monolayers. Under various culture conditions, some of stages 'B' and 'C' buds develop (3-20 days) one or more large (1 mm diameter) spheres. Stage 'D' buds develop monolayers (up to 20% of buds) without going through a sphere phase. Neither spheres nor attached monolayers of epithelium were observed in stage 'A' bud cultures. Spheres grew at a rate of 60 microm in diameter per day using specific medium types and did not attach unless the appropriate substrate was present. When attached, epithelial monolayers expanded at a rate of 200 microm in diameter per day, for 3-15 days, and subsequently detached and died. Sixteen types of media were tested. Medium and substrate combinations were found to determine epithelial lifespan. These results revealed significant improvements in the culture of epithelial monolayers from Botryllus palleal buds. However, an early senescence of the developed epithelial sheets (up to two weeks from onset of appearance) may indicate an internal ageing clock that should be taken into consideration in future approaches.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"25 3-4","pages":"137-48"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11022-004-2087-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25204573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Development and characterisation of pilchard (Sardinops sagax neopilchardus) cell lines derived from liver and heart tissues. 来源于肝脏和心脏组织的沙丁鱼(Sardinops sagax neopilchardus)细胞系的发育和特性。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2003-01-01 DOI: 10.1007/s11022-004-9801-5

Lynette M Williams, Mark St J Crane, Nicholas Gudkovs

{"title":"Development and characterisation of pilchard (Sardinops sagax neopilchardus) cell lines derived from liver and heart tissues.","authors":"Lynette M Williams, Mark St J Crane, Nicholas Gudkovs","doi":"10.1007/s11022-004-9801-5","DOIUrl":"https://doi.org/10.1007/s11022-004-9801-5","url":null,"abstract":"Two cell lines have been established from juvenile pilchards (Sardinops sagax neopilchardus) caught in waters off the Victorian coast of Australia. Following establishment of primary cultures derived from different pilchard tissues, using various cell culture media, a pilchard liver (PL) cell line and a pilchard heart (PH) cell line have been maintained in Eagle's minimal essential medium supplemented with 10% foetal bovine serum for over four years. The cell lines have been cryopreserved in liquid nitrogen and can be recovered from storage with good cell viability. Stock cell cultures have been maintained at 20-22 degrees C on a continuous basis in normal atmosphere (100% air), with weekly subculture at a split ratio of 3:1. The origin of the cell cultures was confirmed by PCR analysis using primers designed to be specific for pilchard mitochondrial DNA. In addition, the liver cell line was cloned and both the parental cell line and clones thereof were shown to be susceptible to a broad range of marine and freshwater viral pathogens of fish.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"25 3-4","pages":"105-13"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11022-004-9801-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25032610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11