{"title":"Sterile polystyrene culture dishes induce transformation of polyps into medusae in Aurelia aurita (Scyphozoa, Cnidaria).","authors":"Klaus Herrmann, Barbara Siefker, Stefan Berking","doi":"10.1007/s11022-004-5592-y","DOIUrl":null,"url":null,"abstract":"<p><p>Polyps of Aurelia aurita produce medusae once a year. Under laboratory conditions this process, termed strobilation, can be induced by lowering the incubation temperature for about two weeks. Here we report a fast induction of strobilation by sterile polystyrene culture dishes. The effect is abolished when the culture dishes are washed twice with hot water prior to the experiment. We recommend that polystyrene cultureware should be pre-washed whenever there is an indication of unusual effects.</p>","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"25 3-4","pages":"135-6"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11022-004-5592-y","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in cell science : an official journal of the Society for In Vitro Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s11022-004-5592-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
Polyps of Aurelia aurita produce medusae once a year. Under laboratory conditions this process, termed strobilation, can be induced by lowering the incubation temperature for about two weeks. Here we report a fast induction of strobilation by sterile polystyrene culture dishes. The effect is abolished when the culture dishes are washed twice with hot water prior to the experiment. We recommend that polystyrene cultureware should be pre-washed whenever there is an indication of unusual effects.