Jozef Oboňa, Katarína Fogašová, Peter Krišovský, Laura Mlynárová, Bożena Sikora, Martin Hromada
{"title":"The first report of human biting by Lipoptena cervi from Slovakia.","authors":"Jozef Oboňa, Katarína Fogašová, Peter Krišovský, Laura Mlynárová, Bożena Sikora, Martin Hromada","doi":"10.17420/ap7001.516","DOIUrl":"10.17420/ap7001.516","url":null,"abstract":"<p><p>The first bite by deer ked Lipoptena cervi (Linnaeus, 1758) on humans has been reported from Slovakia. The host was bitten on the head and neck. Pain and itching appeared immediately after the bite. The skin lesion persisted for several weeks. Although the first documented case of human stings is published here, bites may have occurred in the past, albeit rarely.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 ","pages":"47-49"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro efficacy of different concentrations of lupeol on old world Leishmania donovani.","authors":"Talib F Abbas, Ali Hayder Z","doi":"10.17420/ap7002.523","DOIUrl":"10.17420/ap7002.523","url":null,"abstract":"<p><p>Leishmaniosis is a tropical neglected parasitic disease that is endemic in many countries, including Middle East, with no existing effective vaccines. The bite of female sand-fly transmits the causative agent, Leishmania spp., to humans. High toxicity, resistance and treatment failure of the available chemotherapy against visceral leishmaniosis demands the investigation of new anti-leishmanial compounds. Lupeol is a form of triterpene isolated from several medicinal plants and possesses an antimicrobial property. In this study, cytotoxic effect of lupeol was screened against the mammalian amastigotes form and insect promastigote form of Leishmania donovani, following three cycles of incubation at different concentrations by MTT assay. Results revealed the in vitro anti-leishmanial effect of lupeol on both forms of the parasite where significant decline in promastigotes and amastigotes growth was observed. This was conducted along three times of follow up (24, 48, 72) hours, in comparison to the classical sodium stibogluconate treatment. Cell viability was calculated and the minimum IC50 was detected after 48 hours for amastigotes and 24 hours for promastigotes, 12.125 μM, 102.78 μM, respectively. Given the severity of visceral leishmaniosis and the toxicity of conventional chemotherapies, the anti-leishmanial activity of lupeol suggested a promising compound for additional clinical trials.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"73-79"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141750858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abibatou Konate-Toure, Valérie A Bedia-Tanoh, Fulgence K Kassi, Henriette Vanga-Bosson, Etienne K Angora, Estelle G M Kone, Sebastien A J Miezan, Vincent Djohan, Pulcherie C M Barro-Kiki, Hervé E I Menan, William Yavo
{"title":"Assessment of the performance of five malaria rapid diagnostic tests in health facilities in Abidjan (Côte d'Ivoire).","authors":"Abibatou Konate-Toure, Valérie A Bedia-Tanoh, Fulgence K Kassi, Henriette Vanga-Bosson, Etienne K Angora, Estelle G M Kone, Sebastien A J Miezan, Vincent Djohan, Pulcherie C M Barro-Kiki, Hervé E I Menan, William Yavo","doi":"10.17420/ap7002.525","DOIUrl":"https://doi.org/10.17420/ap7002.525","url":null,"abstract":"<p><p>Regular monitoring of malaria rapid diagnostic tests (RDTs) for the management of uncomplicated malaria in healthcare facilities is a key factor in improving diagnostic quality and ensuring better case management. This study aimed to assess the performance of five RDTs (Standard Q Malaria P.f Ag and Standard Q Malaria P.f/Pan (SD Biosensor, Korea), One Step Malaria HRP2/pLDH (P.f/Pan) (Guangzhou Wondfo Biotech Co., Ltd., China), Malaria Pf/Pan (B&O Pharm, France), and Malaria test P.f/pan (Das Labor, Germany)) in two healthcare facilities in Abidjan. This cross-sectional study was conducted between September and October 2022. Overall, 250 patients suffering from uncomplicated malaria were included with a predominance of female patients (56.6%). The mean age was 22.3 years (SD = 20.6; range, 0.17-73). Of the patients tested, forty-six (46) tested positive for thick smears, reflecting a prevalence of 18.5%. Plasmodium falciparum was the most commonly detected species (93.5%). The geometric mean parasitemia was 6,111.80 parasites/μl (SD = 80,026.93) (range: 116-412461). The sensitivity ranged from 95.24% to 95.65%, whereas the specificity ranged from 93.07 to 94.09% for all five tests evaluated. The false positive rate of the tests was less than 10%. No invalid test results were reported. Two-thirds of P. malariae cases detected by microscopy showed also positive results with all the RDTs. All five RDTs showed 100% sensitivity at low parasitemia levels (< 1,000 parasites/μl blood) including three cases of parasites < 200 parasites/μl blood. This study demonstrated the importance of monitoring the performance of RDTs in clinical samples.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"81-90"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mosquito borne virus USUTU as potential threat to human health.","authors":"Jolanta Morozińska-Gogol","doi":"10.17420/ap7002.524","DOIUrl":"10.17420/ap7002.524","url":null,"abstract":"<p><p>USUV in Europe is detected in vectors (mosquitoes) and has a reservoir in vertebrates. There are known fatal epidemics among birds, especially blackbirds. Currently, USUV also causes rare infections in humans. However, the emergence of clinical cases, including severe neurological symptoms, and the finding of seroprevalence in asymptomatic people (e.g. blood donors, forest workers), indicate that USUV, due to its neurotropism, may become a potential public health problem. Therefore, it is very important to monitor cases infections in humans, migratory and resident birds and other animals that may constitute a reservoir of the virus, but also detection of the virus in mosquitoes (vectors), including alien and invasive species, as well as the impact of climatic factors on the ability to spread the virus in the Europe. There is currently no evidence of virus transmission during transfusion or transplantation, but the potential risk of virus transmission from an asymptomatic blood donor to an mmunocompromised recipient must be considered. Although the occurrence of USUV in European countries is currently not a significant threat, surveillance and screening of blood donors for USUV should be carried out during the period of vector activity and during WNV epidemics, as well as in patients with symptoms of meningitis and encephalitis.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"55-71"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A mammary myiasis caused by Psychoda albipennis (Diptera: Psychodidae) in a dairy cow: first record.","authors":"Kaan Yiğit Emer, Heycan Berk Aydin, Hakan Kanlioğlu, Selin Hacilarlioğlu, Metin Pekağirbaş","doi":"10.17420/ap7001.521","DOIUrl":"https://doi.org/10.17420/ap7001.521","url":null,"abstract":"<p><p>Myiasis is one of the most prevalent ectoparasitic infestation worldwide. In this study, we present a rare case of mammary myiasis caused by the larvae of the Psychoda albipennis species. The larvae emerging from the cows' udder were cleared with lactophenol and 30% KOH. Morphological characteristics and literature reviews indicated that the larvae belonged to the species P. albipennis. As a result of mechanical damage caused by the larvae and the use of medications leading to the atrophy of the udder, the cow was sent for slaughter. Myiasis cases occurring in animals housed under inadequate hygiene conditions have been observed to cause economic losses and even lead to death.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haliz Khalid Hasan, Ahmed Basheer Mohammed, Wijdan Mohammed Salih Mero
{"title":"Detection and molecular identification of Entamoeba species in faecal samples from Duhok province, Kurdistan Region, Iraq.","authors":"Haliz Khalid Hasan, Ahmed Basheer Mohammed, Wijdan Mohammed Salih Mero","doi":"10.17420/ap7002.526","DOIUrl":"10.17420/ap7002.526","url":null,"abstract":"<p><p>The study involved the estimation of the prevalence of Entamoeba spp. using microscopy and molecular techniques among symptomatic outpatients during April 2021 to March, 2022. Stool samples were collected from 2592 outpatients with amoebiasis symptoms of both sexes and different ages (≤ l to 60). Also, 107 stool samples were taken randomly from asymptomatic individuals and examined microscopically to detect infection with Entamoeba spp. the positive specimens were used for molecular analysis with positive symptomatic samples targeting the 18S rRNA gene by nested PCR. Microscopically 21.68% (562/2592) were positive, for Entamoeba spp. Males showed highest infection rate than females (67.43% vs 32.56%). Ages from 1-10 years showed the highest rate (54.09%), and urban inhabitant had somewhat a higher rate than rural one (58.54% vs 41.45%) which was statistically non-significant(P>0.05). Among asymptomatic individuals, 57% (61/107) were positive for Entamoeba spp. Nested PCR analysis yielded 73% positive samples for Entamoeba spp. with a fragment size of 897 bp. Three fragment sizes were produced, for E. histolytica, E. dispar and E. moshkovskii which were 439, 174 and 553 bps, respectively. Single infection occurred with, E. histolytica in 46%, of symptomatic and 6% of asymptomatic cases, E. dispar in 38% of asymptomatic and 10% of symptomatic cases, E. moshkovskii, reported at very low rate among both groups.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"91-101"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular evidence of potential albendazole-resistance in Teladorsagia circumcincta in Iraqi sheep.","authors":"Mirzaei Yousef, Yakhchali Mohammad, Mardani Karim","doi":"10.17420/ap7003.534","DOIUrl":"https://doi.org/10.17420/ap7003.534","url":null,"abstract":"<p><p>Gastrointestinal nematodes infection is one of the major health problems in the livestock industry in many parts of the world and Iraq. Anthelmintic drugs are heavily relied upon to control them so that repeated use lead to development of anthelmintic resistance. The present study was carried out to detect the albendazole-resistance in Teladorsagia circumcincta from Iraqi sheep in Soran district, Kurdistan Region. A total of 140 sheep abomasa were randomly collected. Allele-specific PCR (ASP) was used to investigate the frequency of single nucleotide polymorphisms (SNPs) in codon 200 of isotype I β-tubulin gene. The frequency of the homozygous susceptible (SS), homozygous resistant (rr) and heterozygous susceptible (Sr) were 60.9%, 0.0% and 39.1% in the examined T. circumcincta isolates, respectively. From the results of the present study, it was concluded that T. circumcincta was sensitive to albendazole however; the presence of resistance allele in some populations may lead to the emergence of resistant individuals in the region.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"159-164"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Barbara Paoletti, Raffaella Iorio, Simone Morelli, Lisa Di Teodoro, Elisabetta De Angelis, Roberto Bartolini, Angela Di Cesare
{"title":"A pilot study of the in vitro efficacy of different concentrations of Duddingtonia flagrans for the control of gastrointestinal nematodes of sheep.","authors":"Barbara Paoletti, Raffaella Iorio, Simone Morelli, Lisa Di Teodoro, Elisabetta De Angelis, Roberto Bartolini, Angela Di Cesare","doi":"10.17420/ap7002.528","DOIUrl":"https://doi.org/10.17420/ap7002.528","url":null,"abstract":"<p><p>Duddingtonia flagrans is a nematode trapping fungus used for the control of gastrointestinal nematodes in livestock. The quantity of chlamydospores of D. flagrans required for the reduction of third-stage larvae (L3) of sheep gastrointestinal nematodes (GIN) is largely unknown, and a matter of discussion. The aim of this experiment was to determine in vitro the nematophagous activity of four different concentrations of D. flagrans (1000, 3000, 6250, or 11000 chlamydospores/ml) in the presence of varying numbers of GIN third-stage larvae (L3) (500, 1000, 1500). Additionally, the study sought to evaluate the efficacy of this fungus on Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis and Chabertia ovina. The results showed that as fungal concentrations increased, so did the larval reduction of third-stage infective larvae in each test. L3s number was not a determining factor in the efficacy against GIN. The comparison between various concentrations of chlamydospores revealed significant differences, particularly between 1000 and 11000 chlamydospores (P≤0.05). Regarding the larval reduction of the GIN species considered, D. flagrans demonstrated the same effectiveness across all species tested. The results of the current study confirm the efficacy and underscore the importance of D. flagrans as an alternative for controlling of GIN.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"113-118"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Damanbha Lyngdoh, Goerge C Shabong, Calvin Donkupar Warjri, Philayung Zas
{"title":"Documentation of Radix acuminata as the intermediate host for Fasciola gigantica in Meghalaya, Northeast India.","authors":"Damanbha Lyngdoh, Goerge C Shabong, Calvin Donkupar Warjri, Philayung Zas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fascioliasis, caused by F. gigantica, is a significant parasitic disease affecting livestock and humans. The study investigates the presence of Radix acuminata in local water bodies and its potential to harbor F. gigantica cercariae larvae. The study aims to elucidate the role of Radix acuminata in the transmission cycle of Fasciola gigantica. The taxonomic complexity of Lymnaeids poses challenges, prompting molecular and morphological analysis for accurate species identification. The findings shed light on the transmission dynamics of fascioliasis in this region and emphasize the importance of understanding intermediate hosts for effective control measures. This research contributes to the broader field of parasitology and informs strategies for mitigating the impact of fascioliasis in Meghalaya, Northeast India.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"137-145"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ricardo Souza Oliveira, Thais Avelar Vieira, Silmara Cristina Silva de Aquino, Diego Carvalho Viana, Alana Lislea de Sousa, Germán Augusto Murrieta Morey
{"title":"Presence of Contracaecum sp. larvae in two characiform fish from northeastern Brazil.","authors":"Ricardo Souza Oliveira, Thais Avelar Vieira, Silmara Cristina Silva de Aquino, Diego Carvalho Viana, Alana Lislea de Sousa, Germán Augusto Murrieta Morey","doi":"10.17420/ap7003.532","DOIUrl":"10.17420/ap7003.532","url":null,"abstract":"<p><p>Pygocentrus nattereri Kner, 1858 and Serrasalmus rhombeus (Linnaeus, 1766) are species of serrasalmids sold in open-air markets and market municipalities in Maranhão, Brazil. Fifty-two individuals of P. nattereri and 52 of S. rhombeus were acquired from a fish market in the Municipality of Pinheiro,Maranhão, Brazil, and transported to the laboratory of \"Inmunohistoquímica\" from \"Universidade Estadual do Maranhão\" (UEMA). The lateral musculature of the fish was analyzed by making fine cuts, internal organs were placed in Petri dishes with distilled water, and with the aid of tweezers and needles, the tissue was examined by making fine cuts that allowed observation inside them. The results of the study revealed the presence of nematode larvae L3 of Contracaecum sp. was found parasitizing the visceral cavity, intestines, pyloric cecum, and stomach of P. nattereri and S. rhombeus. This study alerts local authorities and the population to take the necessary measures to prevent the accidental ingestion of endoparasites with zoonotic potential.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"147-154"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}