Annals of parasitology最新文献

Determination of the anti-protozoal activity of medicinal agents using the phenomenon of plaque formation by Acanthamoeba spp. 利用棘阿米巴菌形成斑块的现象测定药剂的抗原虫活性

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7001.522

Volodymyr Shyrobokov, Vadym Poniatovskyi, Valeriia Poniatovska, Anastasiia Romanchyshyna, Rusłan Sałamatin

{"title":"Determination of the anti-protozoal activity of medicinal agents using the phenomenon of plaque formation by Acanthamoeba spp.","authors":"Volodymyr Shyrobokov, Vadym Poniatovskyi, Valeriia Poniatovska, Anastasiia Romanchyshyna, Rusłan Sałamatin","doi":"10.17420/ap7001.522","DOIUrl":"10.17420/ap7001.522","url":null,"abstract":"Representatives of the genus Acanthamoeba are among the most widespread protists in the environment. They have a ubiquitous distribution and can sometimes cause quite serious pathologies in humans. The treatment ofp rotozoal infections caused by free-living amoebae is currently limited and often unsuccessful. In the presented investigation, amebicidal activity was determined against both the trophozoites and cysts of Acanthamoeba spp., which were isolated during the microbiological examination of environmental objects. The inhibitory activity of drugs in vitro was determined using the authors' proposed method, which is based on the plaque formation phenomenon: this is initiated by free-living amoebae when cultured in agar containing the bacteria Cellulosimicrobium sp. strain bent-1. Based on a series of experimental studies, the paper proposes a reliable and inexpensive method for determining the anti-protozoal activity of medicinal agents, which will significantly complement the current screening method system when studying existing drugs, or new drugs during their development stage.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 1","pages":"15-22"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redescription and new geographic distribution record of Dactylogyrus pharyngocephalus (Monogenea: Dactylogyridae) from tank goby Glossogobius giuris (Gobiidae, Teleostei) in Mizoram, northeast India. 从印度东北部米佐拉姆的鰕虎鱼 Glossogobius giuris（戈壁鱼科，远洋鱼类）中重新描述 Dactylogyrus pharyngocephalus（Monogenea: Dactylogyridae）及其新的地理分布记录。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7001.519

Sneha Prakash, Amit K Trivedi, Amit Tripathi

引用次数: 0

The topics of the sixty annual meetings "Clinical Days of Medical Parasitology" (CDMP) Medical University of Lodz. 罗兹医科大学六十届年会 "医学寄生虫学临床日"（CDMP）的主题。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7001.520

Anna Wójcik, Piotr Kurnatowski

引用次数: 0

The first report of human biting by Lipoptena cervi from Slovakia. 斯洛伐克首次报告 Lipoptena cervi 咬伤人类。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7001.516

Jozef Oboňa, Katarína Fogašová, Peter Krišovský, Laura Mlynárová, Bożena Sikora, Martin Hromada

引用次数: 0

In vitro efficacy of different concentrations of lupeol on old world Leishmania donovani. 不同浓度的羽扇豆醇对旧世界利什曼原虫的体外疗效。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7002.523

Talib F Abbas, Ali Hayder Z

{"title":"In vitro efficacy of different concentrations of lupeol on old world Leishmania donovani.","authors":"Talib F Abbas, Ali Hayder Z","doi":"10.17420/ap7002.523","DOIUrl":"10.17420/ap7002.523","url":null,"abstract":"Leishmaniosis is a tropical neglected parasitic disease that is endemic in many countries, including Middle East, with no existing effective vaccines. The bite of female sand-fly transmits the causative agent, Leishmania spp., to humans. High toxicity, resistance and treatment failure of the available chemotherapy against visceral leishmaniosis demands the investigation of new anti-leishmanial compounds. Lupeol is a form of triterpene isolated from several medicinal plants and possesses an antimicrobial property. In this study, cytotoxic effect of lupeol was screened against the mammalian amastigotes form and insect promastigote form of Leishmania donovani, following three cycles of incubation at different concentrations by MTT assay. Results revealed the in vitro anti-leishmanial effect of lupeol on both forms of the parasite where significant decline in promastigotes and amastigotes growth was observed. This was conducted along three times of follow up (24, 48, 72) hours, in comparison to the classical sodium stibogluconate treatment. Cell viability was calculated and the minimum IC50 was detected after 48 hours for amastigotes and 24 hours for promastigotes, 12.125 μM, 102.78 μM, respectively. Given the severity of visceral leishmaniosis and the toxicity of conventional chemotherapies, the anti-leishmanial activity of lupeol suggested a promising compound for additional clinical trials.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"73-79"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141750858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the performance of five malaria rapid diagnostic tests in health facilities in Abidjan (Côte d'Ivoire). 评估阿比让（科特迪瓦）医疗机构中五种疟疾快速诊断检测方法的性能。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7002.525

Abibatou Konate-Toure, Valérie A Bedia-Tanoh, Fulgence K Kassi, Henriette Vanga-Bosson, Etienne K Angora, Estelle G M Kone, Sebastien A J Miezan, Vincent Djohan, Pulcherie C M Barro-Kiki, Hervé E I Menan, William Yavo

{"title":"Assessment of the performance of five malaria rapid diagnostic tests in health facilities in Abidjan (Côte d'Ivoire).","authors":"Abibatou Konate-Toure, Valérie A Bedia-Tanoh, Fulgence K Kassi, Henriette Vanga-Bosson, Etienne K Angora, Estelle G M Kone, Sebastien A J Miezan, Vincent Djohan, Pulcherie C M Barro-Kiki, Hervé E I Menan, William Yavo","doi":"10.17420/ap7002.525","DOIUrl":"https://doi.org/10.17420/ap7002.525","url":null,"abstract":"Regular monitoring of malaria rapid diagnostic tests (RDTs) for the management of uncomplicated malaria in healthcare facilities is a key factor in improving diagnostic quality and ensuring better case management. This study aimed to assess the performance of five RDTs (Standard Q Malaria P.f Ag and Standard Q Malaria P.f/Pan (SD Biosensor, Korea), One Step Malaria HRP2/pLDH (P.f/Pan) (Guangzhou Wondfo Biotech Co., Ltd., China), Malaria Pf/Pan (B&O Pharm, France), and Malaria test P.f/pan (Das Labor, Germany)) in two healthcare facilities in Abidjan. This cross-sectional study was conducted between September and October 2022. Overall, 250 patients suffering from uncomplicated malaria were included with a predominance of female patients (56.6%). The mean age was 22.3 years (SD = 20.6; range, 0.17-73). Of the patients tested, forty-six (46) tested positive for thick smears, reflecting a prevalence of 18.5%. Plasmodium falciparum was the most commonly detected species (93.5%). The geometric mean parasitemia was 6,111.80 parasites/μl (SD = 80,026.93) (range: 116-412461). The sensitivity ranged from 95.24% to 95.65%, whereas the specificity ranged from 93.07 to 94.09% for all five tests evaluated. The false positive rate of the tests was less than 10%. No invalid test results were reported. Two-thirds of P. malariae cases detected by microscopy showed also positive results with all the RDTs. All five RDTs showed 100% sensitivity at low parasitemia levels (< 1,000 parasites/μl blood) including three cases of parasites < 200 parasites/μl blood. This study demonstrated the importance of monitoring the performance of RDTs in clinical samples.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"81-90"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquito borne virus USUTU as potential threat to human health. 蚊子传播的 USUTU 病毒对人类健康构成潜在威胁。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7002.524

Jolanta Morozińska-Gogol

{"title":"Mosquito borne virus USUTU as potential threat to human health.","authors":"Jolanta Morozińska-Gogol","doi":"10.17420/ap7002.524","DOIUrl":"10.17420/ap7002.524","url":null,"abstract":"USUV in Europe is detected in vectors (mosquitoes) and has a reservoir in vertebrates. There are known fatal epidemics among birds, especially blackbirds. Currently, USUV also causes rare infections in humans. However, the emergence of clinical cases, including severe neurological symptoms, and the finding of seroprevalence in asymptomatic people (e.g. blood donors, forest workers), indicate that USUV, due to its neurotropism, may become a potential public health problem. Therefore, it is very important to monitor cases infections in humans, migratory and resident birds and other animals that may constitute a reservoir of the virus, but also detection of the virus in mosquitoes (vectors), including alien and invasive species, as well as the impact of climatic factors on the ability to spread the virus in the Europe. There is currently no evidence of virus transmission during transfusion or transplantation, but the potential risk of virus transmission from an asymptomatic blood donor to an mmunocompromised recipient must be considered. Although the occurrence of USUV in European countries is currently not a significant threat, surveillance and screening of blood donors for USUV should be carried out during the period of vector activity and during WNV epidemics, as well as in patients with symptoms of meningitis and encephalitis.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"55-71"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mammary myiasis caused by Psychoda albipennis (Diptera: Psychodidae) in a dairy cow: first record. 奶牛中由白腹惊魂虫（双翅目：惊魂虫科）引起的乳腺肌炎：首次记录。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7001.521

Kaan Yiğit Emer, Heycan Berk Aydin, Hakan Kanlioğlu, Selin Hacilarlioğlu, Metin Pekağirbaş

引用次数: 0

Detection and molecular identification of Entamoeba species in faecal samples from Duhok province, Kurdistan Region, Iraq. 伊拉克库尔德斯坦地区杜胡克省粪便样本中 Entamoebaspecies 的检测和分子鉴定。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7002.526

Haliz Khalid Hasan, Ahmed Basheer Mohammed, Wijdan Mohammed Salih Mero

{"title":"Detection and molecular identification of Entamoeba species in faecal samples from Duhok province, Kurdistan Region, Iraq.","authors":"Haliz Khalid Hasan, Ahmed Basheer Mohammed, Wijdan Mohammed Salih Mero","doi":"10.17420/ap7002.526","DOIUrl":"10.17420/ap7002.526","url":null,"abstract":"The study involved the estimation of the prevalence of Entamoeba spp. using microscopy and molecular techniques among symptomatic outpatients during April 2021 to March, 2022. Stool samples were collected from 2592 outpatients with amoebiasis symptoms of both sexes and different ages (≤ l to 60). Also, 107 stool samples were taken randomly from asymptomatic individuals and examined microscopically to detect infection with Entamoeba spp. the positive specimens were used for molecular analysis with positive symptomatic samples targeting the 18S rRNA gene by nested PCR. Microscopically 21.68% (562/2592) were positive, for Entamoeba spp. Males showed highest infection rate than females (67.43% vs 32.56%). Ages from 1-10 years showed the highest rate (54.09%), and urban inhabitant had somewhat a higher rate than rural one (58.54% vs 41.45%) which was statistically non-significant(P>0.05). Among asymptomatic individuals, 57% (61/107) were positive for Entamoeba spp. Nested PCR analysis yielded 73% positive samples for Entamoeba spp. with a fragment size of 897 bp. Three fragment sizes were produced, for E. histolytica, E. dispar and E. moshkovskii which were 439, 174 and 553 bps, respectively. Single infection occurred with, E. histolytica in 46%, of symptomatic and 6% of asymptomatic cases, E. dispar in 38% of asymptomatic and 10% of symptomatic cases, E. moshkovskii, reported at very low rate among both groups.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"91-101"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pilot study of the in vitro efficacy of different concentrations of Duddingtonia flagrans for the control of gastrointestinal nematodes of sheep. 关于不同浓度的 Duddingtonia flagrans 对控制绵羊胃肠道线虫的体外药效的试点研究。

Annals of parasitology Pub Date : 2024-01-01 DOI: 10.17420/ap7002.528

Barbara Paoletti, Raffaella Iorio, Simone Morelli, Lisa Di Teodoro, Elisabetta De Angelis, Roberto Bartolini, Angela Di Cesare

{"title":"A pilot study of the in vitro efficacy of different concentrations of Duddingtonia flagrans for the control of gastrointestinal nematodes of sheep.","authors":"Barbara Paoletti, Raffaella Iorio, Simone Morelli, Lisa Di Teodoro, Elisabetta De Angelis, Roberto Bartolini, Angela Di Cesare","doi":"10.17420/ap7002.528","DOIUrl":"https://doi.org/10.17420/ap7002.528","url":null,"abstract":"Duddingtonia flagrans is a nematode trapping fungus used for the control of gastrointestinal nematodes in livestock. The quantity of chlamydospores of D. flagrans required for the reduction of third-stage larvae (L3) of sheep gastrointestinal nematodes (GIN) is largely unknown, and a matter of discussion. The aim of this experiment was to determine in vitro the nematophagous activity of four different concentrations of D. flagrans (1000, 3000, 6250, or 11000 chlamydospores/ml) in the presence of varying numbers of GIN third-stage larvae (L3) (500, 1000, 1500). Additionally, the study sought to evaluate the efficacy of this fungus on Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis and Chabertia ovina. The results showed that as fungal concentrations increased, so did the larval reduction of third-stage infective larvae in each test. L3s number was not a determining factor in the efficacy against GIN. The comparison between various concentrations of chlamydospores revealed significant differences, particularly between 1000 and 11000 chlamydospores (P≤0.05). Regarding the larval reduction of the GIN species considered, D. flagrans demonstrated the same effectiveness across all species tested. The results of the current study confirm the efficacy and underscore the importance of D. flagrans as an alternative for controlling of GIN.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"113-118"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0