Annals of parasitology最新文献

{"title":"Detection of Serpentoplasma sp. (Apicomplexa: Haemohormidiidae) in dice snake (Natrix tesellata) (Colubridae: Natrix) in Azerbaijan.","authors":"Turkan Gurbanova","doi":"10.17420/ap71.553","DOIUrl":"https://doi.org/10.17420/ap71.553","url":null,"abstract":"<p><p>The aim of this study was to determine the presence of blood parasites in dice snake (Natrix tesellata) from Pirallahi Island, located off the western coast of the Caspian Sea of Azerbaijan. The dice snake is one of the most widespread and common species of the herpetofauna of Azerbaijan. This species of snakes represents an important link in the natural balance, since included in various biocenotic connections. A total of 67 specimens of dice snake individuals were captured during spring and summer 2021-2024. Blood sampling was carried out from the caudal vein by insulin syringe. The blood smears were stained with Giemsa solution and examined by light microscopy for haemoparasites. Microscopical examination of the blood smears revealed that 23 snakes of 67 (15.4%) were infected with Serpentoplasma sp. Multiply infected erythrocytes with one to three parasites were unusual, with most host cells containing a single inclusion. Infected thrombocytes were not detected. The parasites contained round-to-oval inclusions from small, anaplasmoid-like bodies, vacuoles with a dot or two of apparent chromatin on their margin or without chromatin dots. The structures were intracytoplasmic, measured 3-3.8 Å~ 2.5-3.3 μm and occurred as rounded vacuole-like structures, one in each infected erythrocyte. This is the first finding of haemoparasites in dice snakes in Azerbaijan.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"113-116"},"PeriodicalIF":0.0,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145740717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient with myelodysplastic neoplasm and giant amoebic liver abscess imported from Italy complicated by intestinal obstruction: a case report and review of the literature.","authors":"Marta Szczygieł, Mateusz Hałas, Marek Spichalski, Maria Piotrowiak, Małgorzata Paul","doi":"10.17420/ap71.551","DOIUrl":"https://doi.org/10.17420/ap71.551","url":null,"abstract":"<p><p>Infection with Entamoeba histolytica is widespread and cosmopolitan, but is particularly common in hot zone countries in areas with poor sanitary and hygienic conditions, a lack of access to safe drinking water, and noncompliance with food hygiene. Currently, no cases of indigenous E. histolytica infections have been registered in Poland; only cases imported from areas with a hot climate zone have been reported. So far, no severe extraintestinal amoebiasis has been diagnosed in Poland in people returning from the Mediterranean area. We report an unusual case of concomitant large liver abscess complicated by small bowel obstruction in an Italian immigrant with chronic myelodysplastic leukaemia who had been in Poland for 10 years. The patient did not present with clinical symptoms of colitis like diarrhoea. The patient had not travelled outside Europe and had never been in tropical areas. The clinical course of the infection, the results of imaging, laboratory, serological and parasitological tests, and the therapeutic methods used are discussed in detail. Attention is drawn to the need to consider amoebiasis and its dangerous complications in the differential diagnosis of abdominal pain and pathological space-occupying lesions in the liver of unknown etiology in people returning from travel to the Mediterranean climate.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Giardia intestinalis diarrhea in a HIV-positive patient with Pneumocystis jiroveci pneumonia, as a possible symptom of IRIS.","authors":"Łukasz Pielok, Stanisław Królak, Monika Lisiecka, Joanna Swarcewicz, Arleta Kowala-Piaskowska","doi":"10.17420/ap71.550","DOIUrl":"https://doi.org/10.17420/ap71.550","url":null,"abstract":"<p><p>Human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS) still have a detrimental effect on public health. Lack of adequate therapy inevitably leads to the decrease in lymphocyte T CD4+ population and emergence of opportunistic infections (OIs) and AIDS-indicators. Pneumocystis jiroveci pneumonia (PjP) is recognized as one of the most common OIs in people living with HIV. Giardia intestinalis is a protozoan parasite, commonly reported throughout the world as the most important non-viral cause of human diarrhea. Immunocompromised patients are a high risk group for parasitic infections. Giardiosis usually is self-limiting, however it can cause severe dehydration and malnutrition, especially in immunocompromised individuals. In this work we described a case of 46-year old men, who stoped ART and expanded IRIS symptoms due to PjP and Giardia intestinalis infections. We concluded that parasitic stool examination in HIV/AIDS individuals should be performed to detect asymptomatic protozoa infections, which can lead to diarrhea during ARV treatment. Moreover, determination of the IRIS risk factors may have a detrimental effect on the prevention of severe complications in patients living with AIDS.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"95-98"},"PeriodicalIF":0.0,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal artificial conditions for keeping and breeding medicinal leeches Hirudo verbana and Hirudo orientalis.","authors":"Ruslan Aminov, Alina Aminova, Alexander Florov, Lyudmyla Makyeyeva","doi":"10.17420/ap71.549","DOIUrl":"https://doi.org/10.17420/ap71.549","url":null,"abstract":"<p><p>The aim of the work is to obtain optimal artificial conditions for the maintenance and breeding of medicinal leeches of the species Hirudo verbana and Hirudo orientalis. 180 medicinal leeches were used in the study: 100 leeches of the H. verbana species and 80 H. orientalis leeches. Four groups of animals were formed: two control and two experimental. Control groups of medicinal leeches were kept using the standard jar method. Experiments according to the developed optimal scheme. As a result of the study, it was found that there were more juveniles obtained from the cocoon per 1 parent leech in the experimental group of medicinal leeches of the H. verbana and H. orientalis species, compared to the control group; appearance of defective cocoons. Mortality of parent leeches during breeding, mortality of juveniles before the first, after the first and sixth feeding decreases on average by more than 50% (P < 0.05) in the experimental group.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoparasites influence on vocalization and mating success of Physalaemus cuvieri Fitzinger, 1826.","authors":"Heitor Tavares de Sousa Machado, Kassio Castro de Araújo, Cristiana Ferreira da Silva, Robson Waldemar Ávila","doi":"10.17420/ap71.543","DOIUrl":"https://doi.org/10.17420/ap71.543","url":null,"abstract":"<p><p>Anurans vocalize in different social contexts, in which the advertisement call is the most disseminated. Different endoparasites usually parasitize these animals, but how these endoparasite infections affect anuran vocalizations remains unclear. Therefore, we investigate how endoparasite infections influence the advertisement call and mating success of Physalaemus cuvieri. For that, we searched and recorded the advertisement call of this anuran at a Caatinga area, during the rainy season from March to April 2021 and February to April 2022, totaling 63 sampling days. After recording the advertisement call, we monitored their success in starting amplexus with females. In the lab, we necropsied all collected individuals to see if some endoparasites infected them. We found a significant effect of the parasite load only on call intervals-more parasitized individuals exhibited higher call intervals. In addition, we observed that the less-weight individuals tended to be more parasitized. Lastly, only the male snout-vent lengths affected the matting success in the studied population of Physalaemus cuvieri. The present study is an essential contribution to understanding how anuran health influences communication and sexual selection.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study on the therapeutic potential of pulsed electric fields (PEF) in hydatid cysts: ex vivo targeted detachment of the germinal layer in daughter cysts.","authors":"Bahman Maleki, Zainab Shankaii, Abolfazl Salehi, Tahereh Mohammadzadeh","doi":"10.17420/ap71.546","DOIUrl":"https://doi.org/10.17420/ap71.546","url":null,"abstract":"<p><p>Cystic echinococcosis (CE), a globally parasitic disease is primarily caused by the larval stage of Echinococcus granulosus. This zoonotic infection carries significant medical, veterinary, and economic implications. In human the disease occurs by the ingestion of parasite eggs and can create in any organ especially liver and lungs. Current therapeutic methods have some limitations. Therefore, effect of pulsed electric fields (PEF) as a non-invasive method was utilized on daughter cysts as an important part of hydatid cyst. Daughter cysts was extracted from sheep liver hydatid cyst, divided into three groups. The first group was exposed to PEF at intensities of 70V/cm, 150 V/cm and 1300V/cm. The second group was treated with albendazole (100 μg/ml). The third group, was exposed with different intensities of PEF (as above) following incubation of daughter cysts with albendazole (100 μg/ml). Structural changes in the daughter cysts were analyzed using a stereomicroscope. The laminated layer remained undamaged in all three groups. In the first group (PEF), some degree of opacity and detachment of germinal layer was observed especially in higher pulse intensity (1300 V/cm). Albendazole treatment (second group) induced significant opacity. In the combined PEF and albendazole (third group), although germinal layer detachment was occurred in addition to opacity but the level of detachment was not similar to those that observed in the first group. The destructive effect of PEF on the germinal layer of daughter cysts can be considered as a promising result in the treatment of CE.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"63-69"},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metazoan endoparasites of Myloplus nigrolineatus (Characiformes: Serrasalmidae) from upper Amazon river basin, Brazil.","authors":"Rafael Braga do Amaral, Guilherme Augustus de Araújo, Luciana Damacena-Silva, Thiago Lopes Rocha, Ricardo Massato Takemoto, Paulo De Marco Júnior","doi":"10.17420/ap71.544","DOIUrl":"https://doi.org/10.17420/ap71.544","url":null,"abstract":"<p><p>The success of Trematoda and Nematoda infection in fish involves a complexity of variables. The objective of this study was to report the parasitological descriptors (prevalence, mean abundance, and mean intensity) and community status of Myloplus nigrolineatus as well as to evaluate the relationship between abundance and richness of endoparasites with biometric parameters, sex, Kn the hosts, percentage cover native vegetation and water temperature. A total of 7,256 endoparasites were found: 861 digeneas and 6,395 specimens of nematodes were collected in the intestine from Myloplus nigrolineatus. One species of Digenea and five species of Nematoda were collected. The initial documentation of the prevalence (%) of endohelminth species in M. nigrolineatus revealed the following: Dadaytrema oxicephala (70%), Chabaudinema americanum (52%), Cucullanus pinnai pinnai (13%), Myleusnema bicornis (65%), Procamallanus (Spirocamallanus) inopinatus (9%) and Rondonia rondoni (35%). The mean intensity and mean abundance were 329.82 ± 416 and 315.48 ± 417, respectively. The trematode Dadaytrema oxycephala was considered a central and dominant species. The total abundance was explained by the variables total length, relative condition factor (Kn), percentage cover native vegetation and water temperature. Fish relative condition factor (Kn) and sex were not influenced by the parasite infection and did not impair the body condition of the hosts. The GLMM showed there is no relationship between abundance and richness of endoparasites with percentage cover native vegetation, while that every twenty centimeters more in the total length of the hosts, the abundance of endoparasites in the intestine increases, approximately, 2 specimens.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"31-48"},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of naturally occuring mixed infection of Theileria lestoquardi and Theileria annulata in buffalo, in Pakistan.","authors":"Abdur Rehman Khan, Sikandar Ali, Zaid Ali Shah, Sarfraz Ur Rahman, Haroon Akbar, Muhammad Imran Rashid","doi":"10.17420/ap71.547","DOIUrl":"https://doi.org/10.17420/ap71.547","url":null,"abstract":"<p><p>Theileriosis caused by Theileria lestoquardi (malignant ovine theileriosis) in sheep and Theileria annulata (topical theileriosis) in cattle, is a tick-borne hemoprotozoal disease that causes major economic losses in animal production caused by a tick-transmitted haemoprotozoan Theileria species, infecting various domestic and wild animals. It is a threat to the buffalo population in Pakistan due to high mortality rates and economic losses. Extensive mixed farming in Pakistan poses threats due to mixed infections and increased mortality rates. The current case report describes the mixed infection and characterization of Theileria lestoquardi with Theileria annulata in a buffalo raised in a mixed farming setup, in Pakistan. A 6.5-year-old female buffalo presented at the Outdoor Hospital exhibiting swollen sub-scapular lymph nodes, pale mucous membranes, a high fever (41.5°C), dullness, depression, shortness of breath, and a decrease in milk production. Investigation through microscopy, hematology, and PCR confirmed that the buffalo was suffering from theileriosis. A Giemsa-stained thin blood smear showed piroplasms indicating theileriosis. Hematological analysis of the blood revealed lower values for hemoglobin and red blood cells (RBCs), while higher counts of lymphocytes, neutrophils, and monocytes were observed during the infection. The PCR performed on the blood sample was positive for Theileria spp. using RLB universal primers and T. annulata-specific primers. The sequence revealed similarity with T. lestoquardi reported in Pakistan, Iran, Tunisia, and India. The buffalo was treated with Bupralex® and Oxtra LA® at a dosage of 1ml/20kg of body weight I/M, respectively, along with supportive treatment. Despite these interventions, the animal died probably due to damage to cardiac muscles. This study reports the first case of T. annulata mixed infection with T. lestoquardi in a buffalo in Punjab, Pakistan. It can be concluded that mixed farming exacerbates the epidemiology and spread of ovine malignant theileriosis and mortalities possibly due to cardiac arrest in mixed infections.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An insight into the role of artificial intelligence in combating malaria: recent developments.","authors":"Heena Sachdeva, Manish Sharma","doi":"10.17420/ap71.545","DOIUrl":"https://doi.org/10.17420/ap71.545","url":null,"abstract":"<p><p>In order to overcome obstacles in diagnosis, surveillance, treatment, and vector control, artificial intelligence (AI) has emerged as a crucial weapon in the fight against malaria. The eradication of malaria has benefited greatly from the exceptional accuracy and efficiency of AI-driven solutions. This review of the literature examines several uses of AI in the fight against malaria, emphasizing new developments. AI-driven solutions have the potential to improve malaria prevention and eradication efforts with sustained innovation and investment, ultimately enhancing global health security. AI is transforming the treatment of malaria by facilitating personalised medicine, speeding up drug discovery, and enhancing diagnostics. AI is improving treatment approaches and tackling the problems caused by drug-resistant malaria parasites through machine learning, deep learning, and in silico drug repurposing. Achieving long-term malaria eradication targets will require sustained investment in AI-driven malaria research. In epidemiological tracking, artificial intelligence (AI) has also become a potent instrument. AI-driven methods offer creative ways to find novel treatment approaches, maximize drug discovery, and forecast the dynamics of malaria transmission, especially in light of the growing resistance of Plasmodium parasites to current medications. Thus, this review paper provides insights into the developments made by AI in combating malaria.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analgesic effect of medicinal leeches in chronic diseases.","authors":"Ruslan Aminov","doi":"10.17420/ap71.542","DOIUrl":"https://doi.org/10.17420/ap71.542","url":null,"abstract":"<p><p>48 young, middle-aged and elderly male and female subjects with various chronic diseases of various body systems were studied under the influence of the complex of biologically active substances of the medicinal leech species Hirudo verbana. It was found that medicinal leeches are able to show strong analgesic effects in various chronic pathologies, regardless of gender and age. The beginning of the pain-relieving effect occurs already after the first attachment, and the maximum effect is manifested after the third and fourth production. In addition to the pain-relieving effect, most of the subjects, regardless of the article and age, felt a strong influx of energy, and after completing the course of productions, the physiological parameters of the blood began to recover and the functionality of the organs began to recover.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"71 ","pages":"27-30"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}