{"title":"A mammary myiasis caused by Psychoda albipennis (Diptera: Psychodidae) in a dairy cow: first record.","authors":"Kaan Yiğit Emer, Heycan Berk Aydin, Hakan Kanlioğlu, Selin Hacilarlioğlu, Metin Pekağirbaş","doi":"10.17420/ap7001.521","DOIUrl":"https://doi.org/10.17420/ap7001.521","url":null,"abstract":"<p><p>Myiasis is one of the most prevalent ectoparasitic infestation worldwide. In this study, we present a rare case of mammary myiasis caused by the larvae of the Psychoda albipennis species. The larvae emerging from the cows' udder were cleared with lactophenol and 30% KOH. Morphological characteristics and literature reviews indicated that the larvae belonged to the species P. albipennis. As a result of mechanical damage caused by the larvae and the use of medications leading to the atrophy of the udder, the cow was sent for slaughter. Myiasis cases occurring in animals housed under inadequate hygiene conditions have been observed to cause economic losses and even lead to death.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haliz Khalid Hasan, Ahmed Basheer Mohammed, Wijdan Mohammed Salih Mero
{"title":"Detection and molecular identification of Entamoeba species in faecal samples from Duhok province, Kurdistan Region, Iraq.","authors":"Haliz Khalid Hasan, Ahmed Basheer Mohammed, Wijdan Mohammed Salih Mero","doi":"10.17420/ap7002.526","DOIUrl":"10.17420/ap7002.526","url":null,"abstract":"<p><p>The study involved the estimation of the prevalence of Entamoeba spp. using microscopy and molecular techniques among symptomatic outpatients during April 2021 to March, 2022. Stool samples were collected from 2592 outpatients with amoebiasis symptoms of both sexes and different ages (≤ l to 60). Also, 107 stool samples were taken randomly from asymptomatic individuals and examined microscopically to detect infection with Entamoeba spp. the positive specimens were used for molecular analysis with positive symptomatic samples targeting the 18S rRNA gene by nested PCR. Microscopically 21.68% (562/2592) were positive, for Entamoeba spp. Males showed highest infection rate than females (67.43% vs 32.56%). Ages from 1-10 years showed the highest rate (54.09%), and urban inhabitant had somewhat a higher rate than rural one (58.54% vs 41.45%) which was statistically non-significant(P>0.05). Among asymptomatic individuals, 57% (61/107) were positive for Entamoeba spp. Nested PCR analysis yielded 73% positive samples for Entamoeba spp. with a fragment size of 897 bp. Three fragment sizes were produced, for E. histolytica, E. dispar and E. moshkovskii which were 439, 174 and 553 bps, respectively. Single infection occurred with, E. histolytica in 46%, of symptomatic and 6% of asymptomatic cases, E. dispar in 38% of asymptomatic and 10% of symptomatic cases, E. moshkovskii, reported at very low rate among both groups.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"91-101"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular evidence of potential albendazole-resistance in Teladorsagia circumcincta in Iraqi sheep.","authors":"Mirzaei Yousef, Yakhchali Mohammad, Mardani Karim","doi":"10.17420/ap7003.534","DOIUrl":"https://doi.org/10.17420/ap7003.534","url":null,"abstract":"<p><p>Gastrointestinal nematodes infection is one of the major health problems in the livestock industry in many parts of the world and Iraq. Anthelmintic drugs are heavily relied upon to control them so that repeated use lead to development of anthelmintic resistance. The present study was carried out to detect the albendazole-resistance in Teladorsagia circumcincta from Iraqi sheep in Soran district, Kurdistan Region. A total of 140 sheep abomasa were randomly collected. Allele-specific PCR (ASP) was used to investigate the frequency of single nucleotide polymorphisms (SNPs) in codon 200 of isotype I β-tubulin gene. The frequency of the homozygous susceptible (SS), homozygous resistant (rr) and heterozygous susceptible (Sr) were 60.9%, 0.0% and 39.1% in the examined T. circumcincta isolates, respectively. From the results of the present study, it was concluded that T. circumcincta was sensitive to albendazole however; the presence of resistance allele in some populations may lead to the emergence of resistant individuals in the region.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"159-164"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Barbara Paoletti, Raffaella Iorio, Simone Morelli, Lisa Di Teodoro, Elisabetta De Angelis, Roberto Bartolini, Angela Di Cesare
{"title":"A pilot study of the in vitro efficacy of different concentrations of Duddingtonia flagrans for the control of gastrointestinal nematodes of sheep.","authors":"Barbara Paoletti, Raffaella Iorio, Simone Morelli, Lisa Di Teodoro, Elisabetta De Angelis, Roberto Bartolini, Angela Di Cesare","doi":"10.17420/ap7002.528","DOIUrl":"https://doi.org/10.17420/ap7002.528","url":null,"abstract":"<p><p>Duddingtonia flagrans is a nematode trapping fungus used for the control of gastrointestinal nematodes in livestock. The quantity of chlamydospores of D. flagrans required for the reduction of third-stage larvae (L3) of sheep gastrointestinal nematodes (GIN) is largely unknown, and a matter of discussion. The aim of this experiment was to determine in vitro the nematophagous activity of four different concentrations of D. flagrans (1000, 3000, 6250, or 11000 chlamydospores/ml) in the presence of varying numbers of GIN third-stage larvae (L3) (500, 1000, 1500). Additionally, the study sought to evaluate the efficacy of this fungus on Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis and Chabertia ovina. The results showed that as fungal concentrations increased, so did the larval reduction of third-stage infective larvae in each test. L3s number was not a determining factor in the efficacy against GIN. The comparison between various concentrations of chlamydospores revealed significant differences, particularly between 1000 and 11000 chlamydospores (P≤0.05). Regarding the larval reduction of the GIN species considered, D. flagrans demonstrated the same effectiveness across all species tested. The results of the current study confirm the efficacy and underscore the importance of D. flagrans as an alternative for controlling of GIN.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 2","pages":"113-118"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Damanbha Lyngdoh, Goerge C Shabong, Calvin Donkupar Warjri, Philayung Zas
{"title":"Documentation of Radix acuminata as the intermediate host for Fasciola gigantica in Meghalaya, Northeast India.","authors":"Damanbha Lyngdoh, Goerge C Shabong, Calvin Donkupar Warjri, Philayung Zas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fascioliasis, caused by F. gigantica, is a significant parasitic disease affecting livestock and humans. The study investigates the presence of Radix acuminata in local water bodies and its potential to harbor F. gigantica cercariae larvae. The study aims to elucidate the role of Radix acuminata in the transmission cycle of Fasciola gigantica. The taxonomic complexity of Lymnaeids poses challenges, prompting molecular and morphological analysis for accurate species identification. The findings shed light on the transmission dynamics of fascioliasis in this region and emphasize the importance of understanding intermediate hosts for effective control measures. This research contributes to the broader field of parasitology and informs strategies for mitigating the impact of fascioliasis in Meghalaya, Northeast India.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"137-145"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ricardo Souza Oliveira, Thais Avelar Vieira, Silmara Cristina Silva de Aquino, Diego Carvalho Viana, Alana Lislea de Sousa, Germán Augusto Murrieta Morey
{"title":"Presence of Contracaecum sp. larvae in two characiform fish from northeastern Brazil.","authors":"Ricardo Souza Oliveira, Thais Avelar Vieira, Silmara Cristina Silva de Aquino, Diego Carvalho Viana, Alana Lislea de Sousa, Germán Augusto Murrieta Morey","doi":"10.17420/ap7003.532","DOIUrl":"10.17420/ap7003.532","url":null,"abstract":"<p><p>Pygocentrus nattereri Kner, 1858 and Serrasalmus rhombeus (Linnaeus, 1766) are species of serrasalmids sold in open-air markets and market municipalities in Maranhão, Brazil. Fifty-two individuals of P. nattereri and 52 of S. rhombeus were acquired from a fish market in the Municipality of Pinheiro,Maranhão, Brazil, and transported to the laboratory of \"Inmunohistoquímica\" from \"Universidade Estadual do Maranhão\" (UEMA). The lateral musculature of the fish was analyzed by making fine cuts, internal organs were placed in Petri dishes with distilled water, and with the aid of tweezers and needles, the tissue was examined by making fine cuts that allowed observation inside them. The results of the study revealed the presence of nematode larvae L3 of Contracaecum sp. was found parasitizing the visceral cavity, intestines, pyloric cecum, and stomach of P. nattereri and S. rhombeus. This study alerts local authorities and the population to take the necessary measures to prevent the accidental ingestion of endoparasites with zoonotic potential.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"147-154"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renata Przydatek-Tyrajska, Katarzyna Bartosik, Aleksandra Sędzikowska
{"title":"Performance of the diagnostic test for Demodex spp. in the context of minimizing the risk of false negative results.","authors":"Renata Przydatek-Tyrajska, Katarzyna Bartosik, Aleksandra Sędzikowska","doi":"10.17420/ap7004.537","DOIUrl":"https://doi.org/10.17420/ap7004.537","url":null,"abstract":"<p><p>Demodex species are permanent parasites of pilosebaceous units infesting humans worldwide. Of the two species associated with the human host, Demodex folliculorum is much more commonly detected and occurs more abundantly in hair follicles and Zeiss glands. Demodex brevis, on the other hand, is mainly associated with the sebaceous and meibomian glands, all over the body. The infestation is usually chronic, and the troublesome ocular and skin symptoms often make it difficult for patients to function in both a social and professional context. Despite this, diagnostics for Demodex spp. are still not routinely performed during ophthalmological and dermatological examinations. We aimed to compare preparations of eyebrow hair, eyelashes, and skin scrapings, and to investigate the correlation with false negative results in the laboratory diagnosis of demodicosis. Direct microscopic examination was applied on 100 patients (81 women and 19 men). The most common ocular and skin symptoms reported by the study participants were eyelid itching and erythema, respectively. Significant differences were found between infested and uninfested patients with regard to the following symptoms: eyelid itching which was more common in infested patients (35.8%) (p = 0.0343) and chalazion occurring only in infested patients (13.2%) (p = 0.0285). The collection of diagnostic material from more than one site e.g. nasolabial folds, eyebrow hairs or eyelashes has a significant effect on the test result. Taking a single type of a sample from a single face zone can lead to false negative results.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 4","pages":"173-180"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Occurrence of helminths in shrews and small rodents on territories transformed by reclamation.","authors":"Vladimir Vasilievich Shimalov","doi":"10.17420/ap7001.517","DOIUrl":"https://doi.org/10.17420/ap7001.517","url":null,"abstract":"<p><p>Shrews and small rodents inhabit the drainage channel banks in reclai med areas and have their own helminth complex. The aim of the study is to conduct the 3rd research period during 2015-2019 the helminth fauna of these animals living on the drainage channel banks of model reclamation systems in Brest Polesie (south-western part of Belarus), to establish the species composition of helminths and the animal infection by these, and to compare the data obtained with previous research periods. 4,000 trap-days were worked out. 151 specimens of shrews of 4 species and 510 small rodents of 8 species were caught. Animals were examined by the method of complete helminthological dissections. Their numbers were 3.78 and 12.75 individuals per 100 trap-days, and helminth infection was 94.7% and 65.9%, respectively. 66 helminth species were found in animals. The dominant helminth species in infection have been identified. The trematode Prosolecithus danubica Tkach et Bray, 1995 (definitive host: common shrew), the acanthocephalans Centhrorhynchus aluconis (Müller, 1780) (larval host: lesser shrew) and Moniliformis moniliformis (Bremser, 1811) (definitive host: striped field mouse) are new species of helminths for Belarus. New hosts in Belarus have been found for 6 helminth species. Shrews and small rodents are involved in the life cycles of bird, non-ruminant artiodactyl and carnivorous mammal helminths. Nine helminth species have significance for medicine and 7 species for veterinary science. The results are compared with data from the 1st (1996-1999) and 2nd (2005-2010) research periods.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jozef Oboňa, Marcela Adamcová, Laura Mlynárová, Veronika Mokránová, Oldřich Sychra
{"title":"Case report, citizen science reveals the occurrence of Botflies (Diptera: Oestridae) in the Czech Republic and Slovakia.","authors":"Jozef Oboňa, Marcela Adamcová, Laura Mlynárová, Veronika Mokránová, Oldřich Sychra","doi":"10.17420/ap7004.538","DOIUrl":"https://doi.org/10.17420/ap7004.538","url":null,"abstract":"<p><p>We present a case of a botfly larva (Gasterophilus intestinalis (De Geer, 1776)) that was removed from a horse's stomach during gastroscopy. Additionally, we provide a detailed yet unconventional perspective on the research of botfly presence in the Czech Republic and Slovakia. Through citizen science, we confirm the occurrence of two species of Hypodermatinae, among which Hypoderma diana Brauer, 1858 is relatively common. In the subfamily Oestrinae, we confirmed the occurrence of three species. Cephenemyia stimulator (Clark, 1815) is very common, which suggests that this species could negatively impact deer populations and their vitality. On the other hand, species reproductively linked to livestock (cattle, sheep, and goats) are relatively rare, which may be associated with the transition to stable-based farming of these animals.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 4","pages":"181-191"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanna Żarnowska-Prymek, Anna Rawska, Jakub Niestępski, Magdalena Pluta, Rusłan Sałamatin
{"title":"Detection of active an Schistosoma haematobium invasion in a patient, based on the microscopic examination of urine.","authors":"Hanna Żarnowska-Prymek, Anna Rawska, Jakub Niestępski, Magdalena Pluta, Rusłan Sałamatin","doi":"10.17420/ap7003.535","DOIUrl":"10.17420/ap7003.535","url":null,"abstract":"<p><p>A parasitological examination of urine from a patient from Cameroon was performed. The eggs of Schistosoma haematobium were observed. Most of the eggs were viable and contained miracidia; these were subjected to observation. These observations may be useful in the diagnosis of schistosomiasis. Supplementary material (video) accompanies this paper, and is available at https://doi.org/10.17420/ap7003.535.S1 .</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"70 3","pages":"165-167"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}