Annals of parasitology最新文献_第10页

Primary muscular hydatidosis in an endemic area of Peru: report of two cases. 秘鲁一个流行地区的原发性肌肉包虫病:两例报告。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6803.470

Plinio Condor, Jose Argandoña, Kevin Flores-Lovon, Dayana Ticona, Nicolás Cuba-Cáceres, Ericson L Gutierrez

引用次数: 0

A zymographic study of metalloproteinase activities in whole cell extracts and extracellular secretions of Leishmania (L.) , L. major and L. infantum from Iran. 伊朗利什曼原虫、大利什曼原虫和婴儿利什曼原虫全细胞提取物和细胞外分泌物中金属蛋白酶活性的酶谱分析。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6803.464

Parvin Pourshahid, Farzaneh Bozorg-Ghalati, Iraj Mohammadpour, Mostafa Alishavandi, Gholam Reza Hatam

{"title":"A zymographic study of metalloproteinase activities in whole cell extracts and extracellular secretions of Leishmania (L.) , L. major and L. infantum from Iran.","authors":"Parvin Pourshahid, Farzaneh Bozorg-Ghalati, Iraj Mohammadpour, Mostafa Alishavandi, Gholam Reza Hatam","doi":"10.17420/ap6803.464","DOIUrl":"https://doi.org/10.17420/ap6803.464","url":null,"abstract":"Leishmaniosis encompasses a group of diseases that is transmitted by sand flies and caused by different species of Leishmania. The skin is the initial organ to be infected by the Leishmania in cutaneous, mucocutaneous and visceral forms of leishmaniosis. The matrix metalloproteinases (MMPs) are capable of degrading all kinds of extracellular matrix (ECM) proteins. The aim of this study was to investigate the protease activity through zymography in cell extracts and extracellular secretions of L. major, L. tropica and L. infantum as three prevalent Leishmania spp. in Iran. The three Leishmania spp. were cultured in RPMI-1640 medium supplemented with fetal calf serum. Promastigotes and axenic amastigotes were harvested and lysed at various phases, and extracellular secretions and cell extracts were collected. Leishmania spp. were proved by targeting kDNA gene. Enzymes were characterized according to gelatin zymography and sensitivity to distinct proteinase inhibitors. We observed proteinase bands with molecular weights (MWs) between 66 to 180 kDa in cellular extracts of axenic amastigotes of L. infantum, L. tropica, and L. major, and from 66 to 92 kDa in extracellular secretions of L. infantum. No proteinase activities were observed in extracellular secretions of axenic amastigotes and in cellular extracts of promastigotes in logarithmic and stationary phases of L. major and L. tropica. Using specific inhibitors, we determined that these proteolytic activities are due to metalloproteases. Our study demonstrated that amastigotes of all three Leishmania spp. have distinct amounts of proteinase activities and therefore can cause various types of lesions and outcomes of the disease.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"569-585"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10537618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Helminth infections of urban hibernating Nyctalus noctula groups (Chiroptera, Vespertilionidae) in Belarus. 白俄罗斯城市冬眠夜行动物群（穴居动物，Vespertilionidae）的螺旋体感染。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6804.497

Vladimir V Shimalov, Viktor T Demyanchik, Viktor V Demyanchik

{"title":"Helminth infections of urban hibernating Nyctalus noctula groups (Chiroptera, Vespertilionidae) in Belarus.","authors":"Vladimir V Shimalov, Viktor T Demyanchik, Viktor V Demyanchik","doi":"10.17420/ap6804.497","DOIUrl":"https://doi.org/10.17420/ap6804.497","url":null,"abstract":"The city of Brest is one of the places in southwestern Belarus where common noctules Nyctalus noctula (Schreber, 1774) spend the winter. The aim of the study is to investigate the helminth infection of urban hibernating groups of this species of bats. The bat carcasses were subjected to a post-mortem examination. Organs and tissues compression was used to examine 26 of common noctules three groups that died in Brest city during the hibernation period from causes beyond our control. All of the examined common noctules were infected with helminths. Eight species of helminths were found: 4 species of trematodes, 1 species of cestode and 3 species of nematodes. Six species were localized in the intestine, 1 species in the stomach and larvae of 1 species in the wall of the stomach. More often, the common noctule was infected with the nematode Molinostrongylus skrjabini Skarbilovich, 1934 (92.3%), the trematodes Paralecithodendrium chilostomum (Mehlis, 1831) (88.5%), Lecithodendrium linstowi Dollfus, 1931 (84.6 %) and Plagiorchis koreanus Ogata, 1938 (84.6%). The finding represents the first record of the trematode P. koreanus in bats in Belarus. The results further represent the first record of N. noctula as host for larvae of nematode Physaloptera myotis (Babos, 1954) in Belarus.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 4","pages":"869-874"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mites and helminths infestation in Lebanese broiler farming systems. 黎巴嫩肉鸡养殖系统中的螨虫和蠕虫侵扰。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6803.466

Houssam Shaib, Youssef Obeid

{"title":"Mites and helminths infestation in Lebanese broiler farming systems.","authors":"Houssam Shaib, Youssef Obeid","doi":"10.17420/ap6803.466","DOIUrl":"https://doi.org/10.17420/ap6803.466","url":null,"abstract":"This study aims at the identification of endo- and ectoparasites in broiler farms while assessing their prevalence in semi-open and closed rearing systems in Lebanon. Seventeen semi-open and six closed broiler farms were investigated in various Lebanese provinces between March and September 2020 and 2021. Intestinal and composite litter samples were collected and observed for morphological identification of prevalent parasites. Results notably revealed the prevalence of mites in all of the surveyed farms. Moreover, mixed mite-helminth infestation was recorded in 100% of the semi-open farms and 50% of the closed ones. The northern fowl mite Ornithonyssus sylviarum was the predominant chicken parasitic mite in both systems. The poultry red mite Dermanyssus gallinae, was present in 6% of the semi-open farms and was not detected in closed farms. The mammalian itching mite Sarcoptes scabiei and the grain mite Acarus siro were also identified and had similar prevalence pattern as the red poultry mite. Eggs and/or larvae stages of one parasitic helminth species, Ascaridia galli, were detected in half of the semi-open and closed farms while adult worms were not present. This study documents for the first time the prevalence of specific endo- and ectoparasites in Lebanese broiler farms, reflecting managerial problems and poor biosecurity practices. Nevertheless, the identification of specific parasites in this work paves the way towards implementing proper control methods against these overlooked, yet devastating, organisms.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"595-603"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10505279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal helminth parasites of greater cane rats (Thryonomys swinderianus) sold at Omagwa BushmeatMarket, Omagwa, Rivers State, Nigeria. 在尼日利亚河流州奥马格瓦奥马格瓦丛林肉市场出售的大蔗鼠肠道寄生虫(Thryonomys swinderianus)

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6803.469

Chidinma C Amuzie, Patience Nwafor, Belema Robert, Godfrey C Akani

{"title":"Intestinal helminth parasites of greater cane rats (Thryonomys swinderianus) sold at Omagwa BushmeatMarket, Omagwa, Rivers State, Nigeria.","authors":"Chidinma C Amuzie, Patience Nwafor, Belema Robert, Godfrey C Akani","doi":"10.17420/ap6803.469","DOIUrl":"https://doi.org/10.17420/ap6803.469","url":null,"abstract":"Greater cane rats (Thryonomys swinderianus) are important sources of bushmeat in southern Nigeria. Here, we collected and examined intestinal helminth parasites of these rats sold at Omagwa bushmeat market, Rivers State, Nigeria. Twelve intestinal tracts of T. swinderianus were purchased from December, 2020 to February, 2021, and transported to the laboratory for examination. Each sample was incised and its contents examined in 0.9% normal saline solution. Parasites encountered were washed in same solution, fixed in 70% ethanol and subsequently identified using taxonomical keys. Prevalence of infection was computed using standard formula for computing parasite ecological parameters. Four parasitic nematodes were isolated. Oesophagostomum venulosum and Strongylus sp. infected six hosts each accounting for a prevalence of 50.0%. Mean intensity of infection was about 12 parasites/infected host for O. venulosum and about 7 parasites/infected host for Strongylus sp. Trichuris paravispicularis had a prevalence of 33.3% and mean intensity of about two parasites/infected host, while Toxocara vitulorum was recovered as a single individual from one host at a prevalence of 8.3%. We conclude that T. swinderianus serves as host to intestinal nematode parasites, and recommend full parasitological investigation of the animal, where possible, to isolate and identify other helminth parasites not reported in the present research.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"623-629"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10505281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rickettsiae among mustelids - new data from south-west Poland. mustelids中的立克次体——来自波兰西南部的新数据。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6803.468

Kacper Jurczyk, Katarzyna Buńkowska-Gawlik, Agnieszka Perec-Matysiak, Joanna Hildebrand

引用次数: 0

First record of Cotylurus metacercariae (Trematoda:Strigeidae) in Biomphalaria straminea (Planorbidae) from Argentina: morphological and molecular identification. 阿根廷水蚤(Planorbidae)中首次记录的囊蚴子叶虫(吸虫目:滴虫科):形态和分子鉴定。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6803.453

María V Fernández, Monika I Hamann, Carolina Davies, Juan J Lauthier, Dora Davies

{"title":"First record of Cotylurus metacercariae (Trematoda:Strigeidae) in Biomphalaria straminea (Planorbidae) from Argentina: morphological and molecular identification.","authors":"María V Fernández, Monika I Hamann, Carolina Davies, Juan J Lauthier, Dora Davies","doi":"10.17420/ap6803.453","DOIUrl":"https://doi.org/10.17420/ap6803.453","url":null,"abstract":"In the study of the biology of trematode species, the knowledge of the larval stages in snail hosts is important to elucidate their complete life cycle. The goal of the present study was to describe a new tetracotyle-type metacercaria found in the freshwater mollusk Biomphalaria straminea sampled in a rice field from Corrientes province, Argentina. To this end, 1,768 snails were collected from the cultivated plots and irrigated channels during the flooding periods (from the time of sowing to soon after rice harvesting) between December 2016 and May 2017. We used morphological and molecular analysis to characterize the tetracotyle-type metacercariae. Its morphological traits and the internal transcribed spacers (ITS1 and ITS2 plus 5.8S; ~1200 pb) from nuclear ribosomal DNA (rDNA) were amplified and sequenced. From 1,768 specimens of B. straminea screened, 52 were found infected with metacercariae of tetracotyle type (2.9%) that were identified as Cotylurus genus. A total of 218 metacercariae were found encysted in the ovotestis or between the mantle and viscera of B. straminea. Bioinformatic analysis showed that the metacercarial rDNA sequences shared 94% identity with those of Cotylurus gallinulae from Mexico and 100% identity with those of Cotylurus sp. from Brazil. In this study, the morphological descriptions are supplemented with the first molecular identification of a metacercaria related to Cotylurus parasitizing planorbids from Argentina. Also, our study provides a new morphological description in B. straminea, thus broadening the geographical distribution. The life cycle of this Cotylurus metacercariae is unknown and there are no reports of adult stages parasitizing waterfowl in Argentina.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"473-481"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10330343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saliva and tear as useful tools for the diagnosis of Toxoplasma gondii in human specimens: a systematic review. 唾液和泪液作为诊断人类刚地弓形虫的有用工具:系统综述。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6802.426

Tooran Nayeri, Shahabeddin Sarvi, Ahmad Daryani

{"title":"Saliva and tear as useful tools for the diagnosis of Toxoplasma gondii in human specimens: a systematic review.","authors":"Tooran Nayeri, Shahabeddin Sarvi, Ahmad Daryani","doi":"10.17420/ap6802.426","DOIUrl":"https://doi.org/10.17420/ap6802.426","url":null,"abstract":"Toxoplasmosis is diagnosed by serology, mainly using invasive specimens such as serum or cerebrospinal fluid. This study aimed to investigate whether saliva and tear samples can be used instead of serum samples to diagnose Toxoplasma gondii. Five English-language databases were checked up to 2021. Other types of non-invasive samples (milk and urine) were excluded from this study. In 15 cross-sectional studies, a total of 4338 saliva samples were examined, out of which 718/3848 (18.66%), 30/200 (15%), and 60/122 (49.18%) samples were positive for anti-T. gondii IgG, IgM, and IgA antibodies, respectively. And for tear samples, a total of 723 samples in cross-sectional studies and 153 cases and 97 controls in case-control studies were included, out of which 199/538 (36.98%) and 155/247 (62.75%) samples were positive for anti-T. gondii IgG and IgA antibodies in cross-sectional studies and 86/128 (67.18%), 1/53 (1.88%), and 78/153 (50.98%) cases and 4/47 (8.51%), 0/47 (0%), and 12/97 (12.37%) controls were positive for anti-T. gondii IgG, IgM, and IgA antibodies in case-control studies, respectively. The results suggested that antibody levels in saliva and tear samples in humans could be useful for the diagnosis of toxoplasmosis, especially ocular toxoplasmosis using tear samples.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":" ","pages":"201-213"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40487030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of interleukin-6 and interleukin-1-β levels in patients with toxoplasmosis. 弓形虫病患者白细胞介素-6和白细胞介素-1-β水平的相关性

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6802.437

Murtada Hafedh Hussein, Moslim Mohsin Khalaf

{"title":"Association of interleukin-6 and interleukin-1-β levels in patients with toxoplasmosis.","authors":"Murtada Hafedh Hussein, Moslim Mohsin Khalaf","doi":"10.17420/ap6802.437","DOIUrl":"https://doi.org/10.17420/ap6802.437","url":null,"abstract":"Toxoplasmosis is an important disease, which is caused by obligate intracellular parasite Toxoplasma gondii. Maternal infection with toxoplasmosis during pregnancy is frequently stimulates the immune response that may cause the fetus to be lost or deformed. A case control study were conducted in Al-Nassiriya province, southern Iraq to measure the interleukin-6 and interleukin-1-β levels. One hundred and twenty seven recurrent aborted women suspected with toxoplasmosis attending to Bent Al-Huda Maternity and Child Hospital for the period from March 2020 to March 2021, and compared to (20) healthy women as a control group. The ages ranged between 16-42 years, 5 ml of blood samples were collected for serum detection positivity of latex agglutination test, Enzyme Linked immune sorbent assay (interleukin-6 and interleukin-1-β). The distribution analysis showed that the highest toxoplasmosis among aborted women compared to healthy control group, the infection rate were (39.37%). Higher percentages of toxoplasmosis (82.35%) was found within the age group (15-25) years. In addition, the results showed a high positive of toxoplasmosis in 43 cases out of 50 cases by latex agglutination test. Also, the result indicated increasing levels of IL-6 and IL-1-β in patients' sera (24.69, 14.21) respectively, compared to healthy women.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":" ","pages":"317-321"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40579629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Promising potent in vitro activity of curcumin and quercetin nano-niosomes against Trichomonas vaginalis. 姜黄素和槲皮素纳米小体体外抗阴道毛滴虫活性研究。

Annals of parasitology Pub Date : 2022-01-01 DOI: 10.17420/ap6802.432

Hajar Ziaei Hezarjaribi, Fatemeh Mollarostami, Pedram Ebrahimnejad, Bahman Rahimi Esboei, Mahdi Fakhar, Zaynab Sadeghi-Ghadi

{"title":"Promising potent in vitro activity of curcumin and quercetin nano-niosomes against Trichomonas vaginalis.","authors":"Hajar Ziaei Hezarjaribi, Fatemeh Mollarostami, Pedram Ebrahimnejad, Bahman Rahimi Esboei, Mahdi Fakhar, Zaynab Sadeghi-Ghadi","doi":"10.17420/ap6802.432","DOIUrl":"https://doi.org/10.17420/ap6802.432","url":null,"abstract":"Trichomonosis, caused by infection with a motile protozoan parasite called Trichomonas vaginalis, is the most common non-viral sexually transmitted disease worldwide. Since the 1960s, metronidazole has been used as a drug of choice. Considering increased resistance to anti-trichomonial drugs, alternative treatments are urgently needed. In this study, the standard strain of T. vaginalis was cultured in TYM medium. Curcumin and quercetin loaded with hyaluronic acid niosomes were prepared by the thin film hydration method. The mean vesicle size, polydispersity index, and zeta potential of each prepared formulation were characterized, and its anti-Trichomonas activity was assessed by concentrations of 0.01, 0.1, 1, 10 and 100 mg/ml. The cytotoxicity effects of the mentioned drugs were determined using a MTT assay on L929 fibroblast cell viability. The particle sizes of curcumin, quercetin, and curcumin-quercetin entrapped modified nano-niosomes were characterised as 243 ± 5.28, 223 ± 7.21 and 266 ± 4.81 nm. The results showed that quercetin and curcumin at a concentration of 100 mg/ml after 24 h had anti-T. vaginalis activity. However, curcumin at a concentration of 100 at time 3h with 97% growth inhibition had better performance than positive control (metronidazole). According to the results of the MTT assay, all drugs, even at the highest concentration (400 mg/ml), had no toxic effect on the fibroblast cell line. According to potent in vitro activity of curcumin and quercetin nanoniosomes against T. vaginalis in comparison with metronidazole, it can be concluded these compounds could be promising therapeutic candidates for trichomonosis in future.","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":" ","pages":"263-273"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40601688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0