Detection of naturally occuring mixed infection of Theileria lestoquardi and Theileria annulata in buffalo, in Pakistan.

Q3 Medicine
Abdur Rehman Khan, Sikandar Ali, Zaid Ali Shah, Sarfraz Ur Rahman, Haroon Akbar, Muhammad Imran Rashid
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引用次数: 0

Abstract

Theileriosis caused by Theileria lestoquardi (malignant ovine theileriosis) in sheep and Theileria annulata (topical theileriosis) in cattle, is a tick-borne hemoprotozoal disease that causes major economic losses in animal production caused by a tick-transmitted haemoprotozoan Theileria species, infecting various domestic and wild animals. It is a threat to the buffalo population in Pakistan due to high mortality rates and economic losses. Extensive mixed farming in Pakistan poses threats due to mixed infections and increased mortality rates. The current case report describes the mixed infection and characterization of Theileria lestoquardi with Theileria annulata in a buffalo raised in a mixed farming setup, in Pakistan. A 6.5-year-old female buffalo presented at the Outdoor Hospital exhibiting swollen sub-scapular lymph nodes, pale mucous membranes, a high fever (41.5°C), dullness, depression, shortness of breath, and a decrease in milk production. Investigation through microscopy, hematology, and PCR confirmed that the buffalo was suffering from theileriosis. A Giemsa-stained thin blood smear showed piroplasms indicating theileriosis. Hematological analysis of the blood revealed lower values for hemoglobin and red blood cells (RBCs), while higher counts of lymphocytes, neutrophils, and monocytes were observed during the infection. The PCR performed on the blood sample was positive for Theileria spp. using RLB universal primers and T. annulata-specific primers. The sequence revealed similarity with T. lestoquardi reported in Pakistan, Iran, Tunisia, and India. The buffalo was treated with Bupralex® and Oxtra LA® at a dosage of 1ml/20kg of body weight I/M, respectively, along with supportive treatment. Despite these interventions, the animal died probably due to damage to cardiac muscles. This study reports the first case of T. annulata mixed infection with T. lestoquardi in a buffalo in Punjab, Pakistan. It can be concluded that mixed farming exacerbates the epidemiology and spread of ovine malignant theileriosis and mortalities possibly due to cardiac arrest in mixed infections.

巴基斯坦水牛自然发生的lestoquardi和环状ileria混合感染的检测。
由羊的lestoquarditheileria(羊恶性卡氏菌病)和牛的环状卡氏卡氏菌(局部卡氏菌病)引起的卡氏卡氏菌病是一种蜱传的血液原虫病,由蜱传的卡氏卡氏菌引起,感染各种家畜和野生动物,给动物生产造成重大经济损失。由于高死亡率和经济损失,它对巴基斯坦的水牛种群构成威胁。由于混合感染和死亡率上升,巴基斯坦广泛的混合农业构成了威胁。目前的病例报告描述了在巴基斯坦混合养殖环境中饲养的一头水牛中lestoquarditheileria与环状梭菌的混合感染和特征。室外医院出现一只6.5岁雌水牛,表现为肩胛下淋巴结肿大、黏膜苍白、高热(41.5°C)、麻木、抑郁、呼吸急促和产奶量减少。通过显微镜、血液学和聚合酶链反应(PCR)进行的调查证实,这头水牛患有痔疮。吉氏染色薄血涂片显示梨状质,表明他们有肠杆菌病。血液的血液学分析显示,在感染期间,血红蛋白和红细胞(rbc)的值较低,而淋巴细胞、中性粒细胞和单核细胞的计数较高。采用RLB通用引物和环孢t特异性引物对血样进行PCR检测,结果均为阳性。该序列显示与在巴基斯坦、伊朗、突尼斯和印度报道的莱斯托夸蒂绦虫相似。水牛分别接受Bupralex®和Oxtra LA®治疗,剂量为1ml/20kg体重I/M,同时给予支持治疗。尽管采取了这些干预措施,但这只动物可能还是死于心肌损伤。本研究报告了巴基斯坦旁遮普省一头水牛中首例环虫与莱斯托夸底体混合感染病例。由此可见,混合饲养加剧了绵羊恶性羊肠菌病的流行和传播,混合感染可能导致心脏骤停死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Annals of parasitology
Annals of parasitology Medicine-Medicine (all)
CiteScore
1.60
自引率
0.00%
发文量
0
期刊介绍: The Annals of Parasitology (formerly Wiadomości Parazytologiczne) is an official, peer reviewed quarterly of the Polish Parasitological Society. The Annals of Parasitology publishes original papers, review articles, short notes and case reports in the fields of parasitology, mycology, and related disciplines. It also accepts interdisciplinary articles, scientific conference proceedings, book reviews. An important mission of our journal is to inform our Readers about the activities of the Polish Parasitological Society and advancement of parasitology both in Poland and elsewhere.
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