Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine最新文献_第8页

[Blood leukocytes are not a primary latent site of cytomegalovirus]. [血液白细胞不是巨细胞病毒的主要潜伏部位]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

H Hackstein, K Böttcher, H Merz, H Kirchner, G Bein

{"title":"[Blood leukocytes are not a primary latent site of cytomegalovirus].","authors":"H Hackstein, K Böttcher, H Merz, H Kirchner, G Bein","doi":"","DOIUrl":"","url":null,"abstract":"Conflicting results do exist regarding blood leukocytes as a site of CMV latency. In an attempt to detect latently infected blood cells we studied CMV major immediate early (MIE) antigen expression in monocytes or mononuclear leukocytes from 80 blood donors (71 seropositive) after in vitro stimulation and subsequent antigen detection by immunocytochemistry (detection limit 1 positive cell/10(6) cells). None of the more than 800 cytospin preparations investigated turned out to be positive. Additionally the granulocyte macrophage colony stimulating factor (GM-CSF)/hydrocortisone (HC) stimulated monocytes were analysed by reverse transcriptase PCR for the presence of MIE mRNA and were also completely negative. In contrast to the negative findings in blood samples, we detected CMV DNA by PCR in 6 out of 20 suddenly deceased individuals in tissue samples of the lung, but not in those of other organs. We conclude that we have no evidence that blood leukocytes are a site of latent CMV. The transmission of CMV by blood transfusions may be mediated by rare phagocytes that transiently bear CMV after uptake from infected endothelial cells.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"235-40"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19936992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Hemocompatibility testing of polymers]. [聚合物的血液相容性测试]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

U T Seyfert, S Perkins, M Kümmel

引用次数: 0

[Quality assurance in transfusion medicine: organization of a quality assurance system exemplified by the Heidelberg University Blood Bank]. [输血医学的质量保证:以海德堡大学血库为例的质量保证体系的组织]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

D Stahl, A Kluge, D Roelcke

引用次数: 0

[Antibody formation after filtered blood cell replacement in hematologic-oncologic patients]. [血液肿瘤患者滤过血细胞置换后抗体的形成]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

D Barz, D Staack, R Lehmann, K Stange, K Schnurstein

引用次数: 0

[Severe neonatal alloimmune thrombocytopenia with delayed antibody detection]. [新生儿同种免疫性血小板减少伴抗体检测延迟]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

A Schabel, A L König, U Brand, M Schnaidt, U Sugg

引用次数: 0

[Fetal weight-adjusted intrauterine IgG therapy in neonatal alloimmune thrombocytopenia]. [胎儿体重调节宫内IgG治疗新生儿同种免疫性血小板减少症]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

G Giers, J Hoch, R Bald, H Bauer, H Kroll, V Kiefel, R E Scharf, P Hanfland, M Hansmann, C Mueller-Eckhardt

{"title":"[Fetal weight-adjusted intrauterine IgG therapy in neonatal alloimmune thrombocytopenia].","authors":"G Giers, J Hoch, R Bald, H Bauer, H Kroll, V Kiefel, R E Scharf, P Hanfland, M Hansmann, C Mueller-Eckhardt","doi":"","DOIUrl":"","url":null,"abstract":"Fetal alloimmune thrombocytopenia is caused by materno-fetal transfer of platelet antibodies. Since the thrombocytopenic fetus is threatened by intracranial hemorrhage, prenatal observation and, if necessary, treatment is required. However, the benefit of therapeutic options, including intravenous IgG (ivIgG), platelet transfusions or fetal IgG transfusions is still controversial. In this study we have evaluated the effect of intrauterine IgG and intraumbilical platelet transfusions on fetal platelet counts. All patients were multiparous women who were immunized against the Zwa antigen during previous pregnancies and had given birth to at least one severely thrombocytopenic infant. First umbilical blood was sampled at the 20th week of gestation. Fetal treatment of IgG was given, on av erage, over 9 weeks. In all cases, fetal IgG levels rose significantly whereas platelet counts did not increase following fetal IgG treatment. We conclude that fetal IgG infusions have no detectable effect on fetal allo-immune thrombocytopenia. Since platelet counts can be very low as early as 20 weeks of gestation, careful fetal monitoring by umbilical blood sampling is essential. Platelet transfusions in short intervals appear to be the only effective regimen to increase platelet counts in thrombocytopenic fetuses at risk.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"160-4"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19938537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Donor exclusions, discarded blood and transfusion unsuited blood conserves of 2,13 million potential blood donors 1991 to 1994]. [排除献血者、丢弃的血液和输血不合适的血液，1991年至1994年的2,13万潜在献血者]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

U Diekamp, W Wehrend, E Marklof, K Kamutzky

{"title":"[Donor exclusions, discarded blood and transfusion unsuited blood conserves of 2,13 million potential blood donors 1991 to 1994].","authors":"U Diekamp, W Wehrend, E Marklof, K Kamutzky","doi":"","DOIUrl":"","url":null,"abstract":"Unlabelled: German authorities plan to legislate transfusion safety. We report on the efficacy of donor selection, unit pre-release testing, quality assurance and look-back efforts (LBE): Among 2,127,102 visits by 503,971 donors are 8.7% first-time donors. 96% of repeaters present our donor ID-card. We defer 5.6% repeat and 18.2% first-time donors, mostly for their own safety. 0.5% of the donors suffer a reaction. 94,000 units (4.7%) are not released due to confidential self-exclusion (1.1%), quality and safety concerns (1.7%), and positive screening tests (1.9%). Only 5.2% positive infectious disease screening tests were confirmed. HIV-prevalence of first-time donors is 0.002%; the HIV seroconversion rate for second-time donors is 0.003%. All other seroconversion rates for HBV, HCV, HIV and lues at subsequent donations are 0.001%. 23 donor-related LBE did not reveal a single HIV-infected recipient. In 106 recipient-related LBE involving 868 donors, we found 5 seroconverted donors (1 HBV, 3 HCV, 1 HIV).Conclusions: Our repeat donors present proper ID. Strict donor selection criteria result in many rejections. Donor reactions are rare. High demands on blood safety and quality result in many discards. In our hands, confidential unit exclusion does not add safety. The HIV prevalence of first-time and repeat donors is equally low. Donor seroconversions are rare. LBE rarely identify infected donors or recipients. Our current high transfusion safety is unlikely to be improved through legislative measures.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"81-92"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19936317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Storage-induced rheologic and biochemical changes in erythrocyte concentrates with added solution and possible correlations]. [添加溶液后红细胞浓缩物储存诱导的流变学和生化变化及其可能的相关性]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

B Mansouri Taleghani, R Grossmann, G Waltenberger, W Geise, R Langer, H A Henrich, D Wiebecke

{"title":"[Storage-induced rheologic and biochemical changes in erythrocyte concentrates with added solution and possible correlations].","authors":"B Mansouri Taleghani, R Grossmann, G Waltenberger, W Geise, R Langer, H A Henrich, D Wiebecke","doi":"","DOIUrl":"","url":null,"abstract":"Rheological integrity is one of the essentials for red blood cells (rbc) in capillary circulation. The influences of metabolical/biochemical changes of rbc on their rheological properties are well known, but (to our knowledge) until now there are no published studies, which cover both quality aspects of packed rbc (prbc) in additive solutions. According to our standard operating procedures (SOP) we prepared prbc in additive solution (SAG-M) and plasma from 16 regular blood donations. The buffy coat was discharged. Following parameters were measured on day 1, 14 and 28: extracellular pH, ATP and 2,3-DPG content of rbc, prbc-viskosity, -filterability, -aggregability and filter clogging rate. For examination of the filterability a 5% rbc-suspension in phoshate buffered solution was prepared, the remaining tests were performed with rbc-samples adjusted to a hematokrit of 40% with fresh frozen autologous plasma. In correlation with the depletion of ATP a decrease of rbc flexibility and changes of shape (for example spherocytosis) are well known from the literature. Our results confirm the assumed time dependent deterioration of rheological patterns of prbc in SAG-M, reflecting in an increase of rbc-viscosity and filter clogging rate and a decrease of rbc-filterability and -aggregability. We also observed the expected decrease of pH, ATP and 2,3-DPG during storage. The influences of biochemical changes on rheological alterations are discussed. Therefore we recommend on demand rheological examinations in addition to metabolical/biochemical analyses for an extended quality assurance program, for instance for testing new additive solutions or major changes of SOP.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"141-4"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19938533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitation of platelet glycoprotein mRNA using the polymerase chain reaction. 应用聚合酶链反应定量血小板糖蛋白mRNA。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

C Seidl, J Siehl, E Seifried

引用次数: 0

[Blood donation after reaching 65 years of age]. [65岁以后献血]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

K Janetzko, R Böcher, K F Klotz, H Kirchner, H Klüter

{"title":"[Blood donation after reaching 65 years of age].","authors":"K Janetzko, R Böcher, K F Klotz, H Kirchner, H Klüter","doi":"","DOIUrl":"","url":null,"abstract":"European regulations for blood donation recommend a maximum donor age of 65 years. On the other hand, the percentage of the population in this age group is rapidly increasing in Western countries, and in autologous blood donation programs this limitation has already been abandoned. In a prospective study we examined blood donation in elderly donors (18 male and 5 female; mean age 65 years, range 64-69) in comparison to a younger control group (15/9; 58, 55-63). All donors were regular blood donors and had donated for at least 3 years. We investigated the exercise capacity before and after donation of 450 ml whole blood by examination of the physical working capacity (PWC) at heart rates of 110/min and 130/min through treadmill exercise testing and determined the blood viscosity. Additionally, whole-blood count, hemoglobin, plasma-ferritin levels and total iron binding capacity were measured immediately after donation and on days 7, 28 and 49. We found a decrease in whole-blood viscosity and a moderate increase in PWC at heart rates of 110/min and 130/min after donation in both groups. Red cell count and values of hemoglobin and ferritin were significantly lower in both groups after donation and returned to pre-donation values by day 49 in the younger control group. We detected no deterioration in exercise capacity after whole-blood donation in elderly blood donors over 65 years when compared with a younger control group. We suggest that blood donation in otherwise healthy persons aged over 65 years should be accepted.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"76-80"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19936316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0