Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine最新文献_第10页

Storage of peripheral blood stem cell samples alters flow cytometric CD34+ results. 外周血干细胞样品的储存改变了流式细胞术CD34+结果。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

K Gutensohn, K Hummel, A Sputtek, U Cassens, P Kühnl

{"title":"Storage of peripheral blood stem cell samples alters flow cytometric CD34+ results.","authors":"K Gutensohn, K Hummel, A Sputtek, U Cassens, P Kühnl","doi":"","DOIUrl":"","url":null,"abstract":"With the use of monoclonal antibodies against the CD34 antigen, flow cytometry (FC) permits rapid assessment of the quality of hematopoietic grafts. We examined PBSC samples (n = 40) to investigate possible influences of storage time and temperature on FC results. After cytapheresis, a sample of the PBSC product was collected and divided into 4 aliquots. Immediate analysis was performed on one aliquot. The other 3 specimen were stored for a) 24 h at room temperature (RT, 20 +/- 2 degrees C), at room temperature with agitation and c) at +4 degrees C. For flow cytometric analyses, samples were labeled with two CD34 markers (HPCA-2, Becton Dickinson Corp. [BD], USA; QBend-10, Immunotech Corp. [IT], Germany). After 24 h CD34+ signals had decreased by 25.4% (BD) and 27.0% (IT) in average, when samples were stored at room temperature in comparison to the results obtained directly after cytapheresis (p < 0.05). At RT in combination with agitation, there was an increase in signal rates compared to baseline values, probably due to binding of CD34 antibodies to myeloid or non-viable cells (+ 9.2% [BD] and 11.2% [IT]). At a storage temperature of +4 degrees C, CD34+ events did not decrease significantly (-0.7% [BD] and -0.2% [IT]). Our data demonstrate that FC results may be influenced by temperature, agitation and storage time.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"170-4"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19831540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Immunologic effects of blood donation]. 【献血的免疫效应】。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

S Lange, J Riggert, A Humpe, J Dittmann, G Simson, M Köhler

引用次数: 0

[Prenatal management of fetuses with alloimmune thrombocytopenia]. [同种免疫性血小板减少症胎儿的产前管理]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

H Kroll, V Kiefel, G Giers, C Kaplan, M Murphy, A Waters, C Mueller-Eckhardt

{"title":"[Prenatal management of fetuses with alloimmune thrombocytopenia].","authors":"H Kroll, V Kiefel, G Giers, C Kaplan, M Murphy, A Waters, C Mueller-Eckhardt","doi":"","DOIUrl":"","url":null,"abstract":"Fetal alloimmune thrombocytopenia is caused by maternal immunization against a fetal platelet alloantigen and transplacental transfer of the antibody into the fetal circulation. Since 10-20% of the fetuses or newborns are threatened by intracranial hemorrhages (ICH) early management is required. Intensive prenatal monitoring should be performed if a maternal HPA-1a antibody is known and a previous infant suffered from thrombocytopenia and/or ICH. Fetal blood sampling (FBS) should be started at 20th to 22nd weeks of gestation to assess fetal phenotype and platelet count. Different concepts to elevate the fetal platelet count have been discussed: corticosteroids, maternal intravenous immunoglobulins (ivIgG), fetal ivIgG and repeated fetal platelet transfusions. In a European survey with data from five centres maternal corticoid treatment and ivIgG infusion were accompanied by increasing fetal platelet counts in only 20 and 24% of the cases, respectively. In fetuses with very low platelet counts only transfusions of compatible platelets in short intervals are able to sustain a safe platelet count. Fetuses with mild thrombocytopenia should be monitored by subsequent FBS since it could be shown that platelet counts tend to decline during gestation. To avoid bleeding complications during and after FBS which was observed in about 5% of the cases every cord vessel puncture should be covered by a platelet transfusion. As no safe and non-invasive therapy exists for fetal alloimmune thrombocytopenia the value of prenatal screening programs in unaffected pregnancies is questionable.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"150-5"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19938535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Lipid peroxidation and hemolysis in HES cryopreserved erythrocytes]. [HES低温保存红细胞的脂质过氧化和溶血]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

R Langer, T Herold, H A Henrich

{"title":"[Lipid peroxidation and hemolysis in HES cryopreserved erythrocytes].","authors":"R Langer, T Herold, H A Henrich","doi":"","DOIUrl":"","url":null,"abstract":"Using malondialdehyde (MDH) as an indicator of lipid peroxidation it was examined, whether oxygen radicals could be an origin of freeze-induced weakness of HES-cryopreserved erythrocytes. Each of 11 erythrocyte suspensions (Hct = 40; HES 200,000/0.62/12.5%; 60 mmol NaCl) was separated into 40 ml samples, cooled down to -196 degrees C und stored at -80 degrees C, finally. Samples were thawed after 1, 2, 3 und 6 months storage and besides that, one sample having remained at -196 degrees C (LN2). The MDH content (1.5 mumol/l Ery unwashed; 0.4 mumol/l Ery washed) amounted to 3.4 mumol/l Ery after LN2 storage, to 4 mumol/l Ery after 1 und to 8 mumol/l Ery after 6 months at -80 degrees C. Similarly, the MDH generation rate at -80 degrees C increased with storage time. The membrane fragility (1 in freshly drawn erythrocytes; 1.3 in erythrocytes out of LN2) rose from 1.6 after 1 month to 2.4 after 6 months. MDH content and membrane fragility were correlated linearly (r = 0.98). It is concluded that increased superoxide formation is mediated by freezed-induced oxidation of Hb-bound Fe. This allows peroxidation of membrane lipids which in consequence causes hemolysis.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"111-6"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19938530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Automated blood component collection with the MCS 3p]. 用MCS 3p自动采集血液成分。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

T Zeiler, V Kretschmer

{"title":"[Automated blood component collection with the MCS 3p].","authors":"T Zeiler, V Kretschmer","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Two new protocols for the MCS 3p cellseparator (Haemonetics) for automated collection of blood components were evaluated.Material and methods: Two units of FFP and one unit of buffy coat free red blood cells (RBC) in additive solution (PAGGS-M) were produced with the D-RBCP protocol. The RBCP-F protocol included additional in-line filtration of the RBC in a closed system. 20 automated blood component collections were performed with each of the two programs. Filtration of the RBC was performed after storage for 24 h at 4 degrees C. Blood cell counts, biochemical characterization of the RBCs (ATP, 2,3-DPG, LDH, HBDH, free Hb, K+, pH) were determined on day 0, 1, 14, 28 and 49.Results and conclusions: Erythroplasmapheresis with both protocols was performed without any technical problems or adverse reactions. The biochemical parameters of the RBC (until 49 days) were as good or even better as with standard preparations (Hb, 2,3-DPG, ATP, LDH, HBDH, hemolysis, K+). Leukocyte and platelet counts in RBC and FFP (D-RBCP) were well within the range requested by German guidelines. Leukocyte reduction of RBC in RBCP-F was adequate but platelets in FFP exceeded the standard values. The extracorporal volume (771 ml in D-RBCP and 632 ml in RBCP-F) was rather large and at least should be adaptable to the donor's individual blood volume.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"145-9"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19938534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Autoimmune hemolytic anemia caused by cold agglutinins of the anti-Pr specificity after rubella infection]. [风疹感染后抗pr特异性冷凝集素所致自身免疫性溶血性贫血]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

A L König, A Schabel, U Sugg, U Brand, D Roelcke

引用次数: 0

Influence of hemodilution on cefuroxime levels and bacterial contamination of intra- and postoperative processed wound blood during hip replacement. 血液稀释对髋关节置换术中及术后处理伤口血液头孢呋辛水平及细菌污染的影响。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

K H Wollinsky, M Büchele, M Oethinger, P Kluger, H H Mehrkens, R Marre, W Puhl

{"title":"Influence of hemodilution on cefuroxime levels and bacterial contamination of intra- and postoperative processed wound blood during hip replacement.","authors":"K H Wollinsky, M Büchele, M Oethinger, P Kluger, H H Mehrkens, R Marre, W Puhl","doi":"","DOIUrl":"","url":null,"abstract":"Materials and methods: In a prospective randomized study we investigated the effect of hemodilution on cefuroxime levels and bacterial contamination of processed shed blood during hip arthroplasty. 10 patients received cefuroxime 1,5 g as single shot prophylaxis before (group A), 10 after hemodilution (15 ml/KG) (group B). Cefuroxime levels in serum 1 h after administration, at the end of operation, after 12 h and in drainage-blood after 12 h were assessed by HPLC. Bacteriological study was performed from collecting bags (Vacufix), wound drainage blood and processed red blood cell concentrates, using pour plate technique and broth culture enrichment.Results and discussion: Mean concentrations of cefuroxime were higher in group B than group A, but differed not significantly. No bacterial contamination was found in collecting bags and wound drainages in both groups. Processed red cell concentrates in group B showed no growth. In group A, however, 3/10 were contaminated with < or = CFU/ml of coagulase-negative Staphylococcus, Staphylococcus epidermidis and Propionibacterium acnes. The differences between groups did not reach the level of statistical significance and could not be related to lower cefuroxime levels. No wound infection occurred in either group.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"191-5"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19831543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of IgG subclasses on DAT positive RBC membranes by flow cytometry (FC). 流式细胞术(FC)快速检测DAT阳性红细胞膜上IgG亚类。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

C Asmussen, K Gutensohn, D Wittkopf, P Kühnl

{"title":"Rapid detection of IgG subclasses on DAT positive RBC membranes by flow cytometry (FC).","authors":"C Asmussen, K Gutensohn, D Wittkopf, P Kühnl","doi":"","DOIUrl":"","url":null,"abstract":"Unlabelled: Red blood cell (RBC)-bound IgG can cause hemolysis resulting, e.g. in severe cases of autoimmune hemolytic anemias (AIHA) or in hemolytic disease of the newborn (HDN). Serologic detection and differentiation of these antibodies are often difficult in cases of low antibody titers. We investigated 36 cases of poly- and monospecific IgG-positive DATs by flow cytometry. The RBC samples were washed, diluted, and incubated with monoclonal antibodies directed against IgG1, IgG2, IgG3, and IgG4, respectively. Analysis was performed on a flow cytometer. Nine cases were negative for all 4 IgG subclasses, 8 cases were positive for IgG2, 5 for IgG1, 5 for IgG3, and 3 for all four subclasses. In 6 patients we found combinations of 2 or 3 subclasses (2 for IgG1 and IgG2, 1 for IgG1 and IgG3, 1 for IgG3 and IgG4, 1 for IgG1, IgG2 and IgG4, 1 for IgG1, IgG3 and IgG4). Serological differentiation revealed specific anti-bodies only in 3 cases (anti-Lea, anti-Leb, anti-P1). The type of IgG subclass and the amount of RBC-bound IgG is relevant for the degree of in vivo RBC destruction. Flow cytometry provides a rapid, highly sensitive, cost efficient, and specific tool for IgG detection, including subgroups.Conclusions: We therefore recommend flow cytometric analysis to be integrated into the serological decision process as an additional method for serological problem cases.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"35-9"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19832202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Elimination of hydroxyethyl starch after autologous retransfusion of cryopreserved erythrocytes]. [自体低温红细胞回输后羟乙基淀粉的消除]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

A Sputtek, K Gutensohn, R Langer, R Hammes, U H Warnken, H A Henrich, P Kühnl

{"title":"[Elimination of hydroxyethyl starch after autologous retransfusion of cryopreserved erythrocytes].","authors":"A Sputtek, K Gutensohn, R Langer, R Hammes, U H Warnken, H A Henrich, P Kühnl","doi":"","DOIUrl":"","url":null,"abstract":"In the case of the biodegradable cryoprotectant hydroxyethyl starch (HES) no deglycerolization process is required prior to the transfusion of frozen red blood cells (RBC). In a first study the elimination of an HES 200,000/0.62 from the plasma of 6 dogs was investigated by means of a novel HPLC-GFC method. 16% of the blood volume were replaced by autologous HES protected frozen/thawed RBC. In a second study the HES concentration in the plasma of 7 healthy volunteers was determined following the substitution of 8% of the blood volume, but a washing step has been performed to reduce the concentration of the cryoprotectant (HES 200,000/0.5). In a third study, however, this step was omitted. The elimination of the HES followed always first order kinetics. In the case of the transfusions without postthaw washing in dogs and humans, the initial plasma concentrations amounted to 2.11 +/- 0.15 g/dl and 0.75 +/- 0.26 g/dl, respectively. The corresponding value for the washed preparations was less than 0.03 g/dl. Within 4-5 h the concentrations dropped to less than 50% of the initial values. The 9-hour value was less than 35% (dogs), the 20-hour value about 15% (humans) of the initial concentration. As HES is primarily eliminated via the kidneys, within this period the concentrations of HES in the urine dropped from 4.3 +/- 2.11 g/dl to less than 0.03 g/dl (humans, no washing step). In conclusion, the elimination of the accompanying cryoprotectant HES was no problem in the concentrations applied. A simple washing step with isotonic saline, however, effectively reduced the concentration of the extracellular cryoprotectant HES far below critical levels.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"33 ","pages":"215-9"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19936478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HCV transmission through blood transfusion: are HCV-RNA titer in donor serum and genotype major determinants of infection outcome? 丙型肝炎病毒通过输血传播:供者血清中的丙型肝炎病毒rna滴度和基因型是感染结果的主要决定因素吗?

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1996-01-01

M S Cardoso, K Koerner, S Epple, T Dengler, M Kerowgan, B Kubanek

引用次数: 0