{"title":"Collagen type III and type IV detection in and around human hepatocellular carcinoma.","authors":"M V Gulubova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Extracellular matrix proteins participate in tumor cell growth and progression. Their role in the extratumoral liver tissue needs to be elucidated. Eight patients with hepatocellular carcinoma on noncirrhotic livers are investigated by means of light microscopical and ultrastructural immunohistochemistry for collagen type III and type IV. In the tumor collagen type III, staining is weaker, and collagen type IV is increased. It is topographically located near perisinusoidal stromal cells. In the extratumoral liver tissue, the immunostaining for the two antibodies is stronger perisinusoidally. The number of Ito cells increases significantly in the extratumoral liver tissue. A lot of transitional cells are found there. Sinusoids in the extratumoral tissue are dilated and filled with lymphoid cells and platelets. The presence of matrix proteins between tumor cells is necessary to regulate their growth and differentiation. The increase in extracellular matrix content perisinusoidally in the extratumoral tissue probably erects a protective barrier against metastasizing tumor cells. There, collagen type III and type IV accumulation is probably initiated by signals coming from tumor cells, or from inflammatory cells and platelets in sinusoids.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"155-63"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Primary malignant ectomesenchymoma of the orbit.","authors":"A Bittinger, C Rossberg, M Rodehüser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Malignant ectomesenchymoma is a rare soft tissue tumor of the childhood believed to arise from a remnant of pluripotential migratory neural crest cell (ectomesenchym) and composed of both a mesenchymal element (most often embryonal rhabdomyosarcoma) and a neuroectodermal element (ganglioneuroma, schwanomma neuroblastoma or melanocytic cells). Reported sites of origin are the abdomen, perineum or scrotum, the extremities, the middle ear, nasopharynx, face, and neck. Herein we report a new case of an orbital ectomesenchymoma studied by means of histochemistry and immunohistochemistry in order to increase the morphologic and histogenetic knowledge of this peculiar tumor and its significance concerning the differential diagnosis.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"221-5"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20021300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exuberant fibroadenomatoid proliferation in a pulmonary mesenchymoma (hamartoma): report of a lesion mimicking a sclerosing pneumocytoma.","authors":"W Grayson, G Leiman, K Cooper","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report the cytologic and histopathologic features of an unusual peripheral primary lung tumor in a 61-year-old female. The central portion showed features of typical pulmonary chondroid hamartoma (mesenchymoma). Arising from the peripheral margin of this tumor, however, was an exuberant proliferation of papillary mesenchymal structures lined by type 2 pneumocates, mimicking papillary sclerosing hemangioma (pneumocytoma). This extreme example of fibroadenomatoid proliferation shares histologic features with other pulmonary neoplasms characterized by mesenchymal cores lined by type 2 pneumocytes. We propose that such lesions be unified under the term \"alveolar mixed tumor\".</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"247-52"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20021305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Primary extraskeletal mesenchymal chondrosarcoma in fossa poplitea of a 93-year-old woman.","authors":"M Guschmann, I Melcher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report the case of a 93-year-old female who had developed a primary extraskeletal chondrosarcoma in Fossa poplitea for many years. This rare, highly malignant tumor occurred in unusual location and at unusual age. Histologically, it presented with sheets of primitive mesenchymal cells and islets of well-differentiated cartilaginous tissue. Immunohistochemical preparations revealed a positivity for neuron-specific enolase and vimentin, the cartilaginous portion of the tumor and isolated cells of the undifferentiated area also stained partially for S-100 protein. Three years later, only one metastasis was found in the contralateral lower extremity.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"119-24"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19913720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunocompetent cells in benign and malignant salivary gland tumors.","authors":"V J Kärjä, K J Syrjänen, S M Syrjänen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>IgA-, IgG, and IgM-producing plasma cells as well as 3- and T-lymphocytes were immunophenotyped and quantitated in a series of 216 benign and malignant salivary gland tumors, with special emphasis placed on the clinical behavior of the tumors. Highest number of plasma cells were found in mucoepidermoid carcinomas, where IgG-plasma cells were the sole Ig-class secreted. No IgA-immunoreactivity was found in adenoid cystic, undifferentiated, acinic cell, carcinoma in pleomorphic adenoma, and mucoepidermoid carcinomas. In squamous cell carcinomas, the number of IgM-plasma cells was higher than that in other salivary gland tumors. Basal cell adenomas contained only IgM-positive plasma cells. In logistic regression analysis, IgG- and IgM-producing plasma cells in malignant salivary gland tumors were related to an increased tumor diameter (p = 0.022 and 0.046, respectively). In benign tumors, neither clinical nor prognostic value could be attributed to the distribution of plasma cells. T-cells and B-cells were present in 63.9% and 33.8% of all tumors, found in 63.8% and 26.7% (p = 0.0048) of the benign tumors, and in 64.1% and 41.7% (not significant) of the malignant tumors, respectively. The presence of T- of B-lymphocytes was of no prognostic significance in malignant tumors. In benign tumors, however, the mean age of the patients was significantly higher (p = 0.010) and the mean time to recurrence significantly shorter (p = 0.018) in patients with tumors containing T-cells than in those devoid of these cells. In conclusion, the cell-mediated immunity (T-cells and their subsets) seems to play a more important role in pathogenesis and prognostication of salivary gland neoplasms than do the cells of the B-cell lineage, and, clearly, further studies are needed to elucidate these issues.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19914347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Agapitos, N Kavantzas, M Bakouris, P M Pavlopoulos, K Kassis, C Liapis, M Sechas, P Davaris
{"title":"Estimation of the percentage of carotid atheromatous plaque components and investigation of a probable correlation with the neurologic status of the patients.","authors":"E Agapitos, N Kavantzas, M Bakouris, P M Pavlopoulos, K Kassis, C Liapis, M Sechas, P Davaris","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study was to estimate the percentage of the carotid atherosclerotic plaque components using image analysis in 50 cases of carotid endarterectomies as well as the search for a probable correlation with the neurologic status of the patients. According to their preoperational neurologic status, the patients were classified as asymptomatic (Group I), symptomatic-TIA (Group II) and symptomatic-previous stroke (Group III). For the image analysis and the exact estimation of the carotid plaque components, special algorithms were developed. The following plaque components were measured: fibrous component, calcium, cellular infiltrations, cholesterol and hemorrhage. Between the groups I-III, there was not found any statistically significant difference of the mean value of all the estimated parameters. Our results indicate that the morphology of the carotid atheromatous plaque seems not to be correlated with the neurologic status of the patients.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"105-8"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19913717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y S Chae, T Flotte, D W Hsu, F Preffer, E T Hedley-Whyte
{"title":"Flow cytometric DNA ploidy and cells phase fractions in recurrent human pituitary adenomas. A correlative study of flow cytometric analysis and the expression of proliferating cell nuclear antigen.","authors":"Y S Chae, T Flotte, D W Hsu, F Preffer, E T Hedley-Whyte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Flow cytometric analysis was applied to embedded tissue to measure the proliferative activity and the DNA ploidy of 16 recurrent and 17 nonrecurrent pituitary adenomas. The results were compared with data from a previous study which demonstrated that proliferating cell nuclear antigen (PCNA) labeling index was higher in recurrent adenomas than in nonrecurrent adenomas. Flow cytometric analysis as a tool for predicting aggressive behavior has been useful in a variety of human tumors; however, its prognostic value in pituitary adenomas is controversial. Therefore, we decided to explore the relationship of the results of flow cytometry and proliferating cell nuclear antigen labeling indices with the prognosis of pituitary adenomas. Three out of 16 recurrent adenomas and five out of 17 nonrecurrent adenomas demonstrated a DNA aneuploid pattern. All the nonfunctional recurrent adenomas had a diploid pattern, while only 40% of the functional recurrent adenomas had a diploid pattern. The GO/G1 phase fraction was higher in the recurrent adenomas, than in the nonrecurrent ones (p = 0.0005). In contrast, the S-phase fraction and the coefficient of variation were higher in the nonrecurrent adenomas (5.9 +/- 1.0%, 7.0 +/- 0.75, respectively) than in the recurrent ones (2.5 +/- 0.6%, 4.0 +/- 0.2%, respectively) (p = 0.003 and p = 0.001, respectively). The proliferating cell nuclear antigen labeling indices were higher in the recurrent adenomas (18.9 +/- 4.5%) than in the nonrecurrent adenomas (2.6 +/- 1.6%) (p = 0.003). The S-phase of flow cytometry correlated weakly with the proliferating cell nuclear antigen labeling indices when the recurrent and the nonrecurrent adenomas were considered as one group. (r = -0.356, p = 0.033). But no significant correlations were observed when the groups of recurrent (r = -0.311, p = 0.195) and nonrecurrent tumors (r = -0.019, p = 0.942) were compared separately. The results of flow cytometric analysis suggest that recurrent adenomas may have a higher proportion of cells in the presynthetic phase than the nonrecurrent adenomas. This study suggests that flow cytometric analysis is of limited value in predicting recurrence of pituitary adenomas.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"89-95"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19913714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical importance and prognostic value of the image-DNA-cytometry for patients with gastric cancer.","authors":"H Stratmann, D Decker, A Hirner, R Bollmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Image-DNA-cytometric analysis was performed retrospectively on air-dried touch preparations from gastric carcinoma from 122 patients who underwent surgery from 1989-1994. The results of DNA cytometry were compared with conventional histologic staging and grading. Aneuploidy could be verified in 81 cases (66.3%), and a significant correlation was demonstrated between aneuploidy and a high incidence of lymph node metastases, whereas no correlation with tumor type and histologic grade was detected. Thus DNA ploidy may by useful in predicting prognosis in gastric cancer.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"97-100"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19913715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Alujevic, D Primorac, S Andelinovic, B Kruslin, M Dominis, S Nigojevic, M Biocic, G Dodig, L Kapural
{"title":"Ciliated metaplasia in a patient of Mediterranean origin with gastric adenoma.","authors":"A Alujevic, D Primorac, S Andelinovic, B Kruslin, M Dominis, S Nigojevic, M Biocic, G Dodig, L Kapural","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report here the first case of ciliated gastric metaplasia in a Croatian patient. This is also the first case of ciliated metaplasia reported in a patient of Mediterranean descent. Cilia were found in slightly cystically dilated gastric glands underneath a gastric adenoma with severe dysplasia. They were visualized by desmin immunohistochemical stain. Cells that presented with cilia were columnar cells, some of them with vacuolization of the cytoplasm. This case report shows that ciliated metaplasia occurs in patients of Southern European origin.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"113-7"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19913719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Paraskevakou, N Kavantzas, P M Pavlopoulos, A Delibasis, D Yova, P Davaris
{"title":"Computerized nuclear morphometry of renal cell carcinomas.","authors":"E Paraskevakou, N Kavantzas, P M Pavlopoulos, A Delibasis, D Yova, P Davaris","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study was the evaluation of the usefulness of nuclear morphometry in the pathology of renal cell carcinoma by computer-aided image analysis and the statistical comparison of nuclear morphometric parameters with the histologic grade of the tumors. 44 cases of renal cell carcinoma, classified by two independent pathologists into 4 histologic grades (I-IV), were examined. The following 5 nuclear morphometric parameters were measured in a large number of randomly selected nuclei of each case: Major axis length, area, elongation, roundness and compactness. The statistical evaluation was performed using one-way analysis of variance between the four groups of the histologic grades (I-IV). Between them, there was a statistically very significant difference of the mean value of all the evaluated parameters. The values of the estimated parameters, with the exception of roundness and compactness, showed a strong tendency to increase in proportion to histologic grade. Our results suggest that image analysis is a reproducible and objective method for the grading of renal cell carcinoma, and it can be helpful in the unbiased evaluation of such tumors.</p>","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 2","pages":"101-4"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19913716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}