General & diagnostic pathology最新文献_第8页

Myofibroblasts and extracellular matrix glycoproteins in palmar fibromatosis. 掌纤维瘤病中的肌成纤维细胞和细胞外基质糖蛋白。

General & diagnostic pathology Pub Date : 1997-02-01

G Magro, F Fraggetta, A Colombatti, S Lanzafame

{"title":"Myofibroblasts and extracellular matrix glycoproteins in palmar fibromatosis.","authors":"G Magro, F Fraggetta, A Colombatti, S Lanzafame","doi":"","DOIUrl":"","url":null,"abstract":"We investigated immunohistochemically the presence of myofibroblasts and their surrounding extracellular matrix (ECM) in palmar fibromatosis nodules, obtained from 30 patients. Histologically, the three following phases were identified in palmar fibromatosis nodules: proliferative, involutional, and residual. A variable association of these phases was simultaneously detected within the same nodule. In most cases (20/30), we found that involutional and residual phases coexisted, while the association of proliferative/involutional/residual and proliferative/involutional phases was observed in 8 and 2 cases, respectively. Alpha-smooth muscle actin, a reliable marker for myofibroblastic cellular phenotype, revealed that most spindle-shaped cells of cellular areas of both proliferative and involutional phases were myofibroblasts. Regardless of the predominant association of histologic phases, these findings demonstrate that a myofibroblastic cellular component is constantly found in palmar fibromatosis nodules, although its degree is variable. Immunohistochemical study of ECM showed that collagen types IV, VI, laminin, and fibronectin were strongly expressed and restricted to cellular areas of involutional and residual phases. A combined study of cellular phenotype and ECM, using consecutive sections, demonstrated that the distritubion of collagen types IV, VI, laminin, and fibronectin followed the distribution of myofibroblasts, while they were not expressed in the areas devoid of these cells. These findings suggest that interactions between myofibroblasts and these surrounding ECM glycoproteins may be involved in the pathophysiology of palmar fibromatosis.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"185-90"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical detection of vinculin in human rhabdomyosarcomas. 人横纹肌肉瘤中血管素的免疫组化检测。

General & diagnostic pathology Pub Date : 1997-02-01

T Meyer, U Brinck

{"title":"Immunohistochemical detection of vinculin in human rhabdomyosarcomas.","authors":"T Meyer, U Brinck","doi":"","DOIUrl":"","url":null,"abstract":"The microfilament-associated protein vinculin is a major constituent of muscle tissue localized in costameres and Z-discs of the sarcomeric apparatus, where it is thought to play a pivotal role in the alignment of sarcomeric myofibrils and the transduction of mechanical force between the internal contractile machinery and the extracellular environment. In order to investigate whether anti-vinculin antibodies are helpful in confirming the commitment of rhabdomyosarcomas to the myogenic pathway, we studied immunohistochemically the expression pattern of vinculin in a series of 7 human rhabdomyosarcomas including those of embryonal, botryoid, and pleomorphic subtypes. Using monoclonal antibody from clone hVIN-1 by APAAP techniques on formalin-fixed, paraffin-embedded tissue, all but one tumor, which was a primitive embryonal rhabdomyosarcoma, demonstrated a significant positive vinculin staining. Vinculin expression was most prominent in differentiated tumors with a focal staining pattern showing a high degree of correlation with rhabdomyoblasts, whereas a diffuse staining was observed in areas in which small, poorly differentiated tumor cells alone were present. Since vinculin immunoreactivity could also be demonstrated in cases of leiomyosarcoma, the positive immunohistochemical detection of vinculin was not exclusively restricted to mesenchymal tumors derived from sarcomeric muscle tissue. Immunodetectable amounts of nebulin could be revealed only in two embryonal rhabdomyosarcomas. Our results suggested that the positive identification of rhabdomyosarcoma achieved by using antibodies against vinculin in addition to other known myogenic markers may be particularly useful in the differential diagnosis of anaplastic, poorly differentiated sarcomas.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"191-8"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical localization of integrins and basement membrane components in colorectal epithelial tumors correlates with their histologic architecture. 结直肠上皮肿瘤中整合素和基底膜成分的免疫组织化学定位与其组织结构相关。

General & diagnostic pathology Pub Date : 1997-02-01

S Ban, A Takahashi, M Takahama

{"title":"Immunohistochemical localization of integrins and basement membrane components in colorectal epithelial tumors correlates with their histologic architecture.","authors":"S Ban, A Takahashi, M Takahama","doi":"","DOIUrl":"","url":null,"abstract":"We studied the immunohistochemical localization of integrins (alpha2, alpha6) and basement membrane components (type IV collagen, laminin) in colorectal epithelial tumors (adenomas and adenocarcinomas) with respect to their intramucosal and invasive growth. The integrins showed two patterns of localization: basal polarization and diffuse expression. In intramucosal tumors, the basement membrane components were well preserved, and the staining intensity for the basal polarization of alpha6 increased with increasing size and grade of dysplasia of the tumors. In invasive adenocarcinomas, the basement membrane components were preserved, and the basal polarization of alpha6 was apparent in well-differentiated areas, while the tumor cells showed disorganization or loss of the basement membrane components and the diffuse expression of alpha6 with a reduction or loss of the basal polarization in moderately and poorly differentiated areas. The expression of alpha2 did not correlate with the grade of dysplasia of intramucosal tumors or the pattern of differentiation of invasive adenocarcinomas. These results suggest that the basal polarization of alpha6 and the preservation of the basement membrane components contribute to the glandular architecture of colorectal epithelial tumors, and that the diffuse expression of alpha6 with a reduction or loss of the basal polarization, and the disorganization and loss of the basement membrane components to the dispolarized affinity of tumor cells to the extracellular matrix.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"165-73"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleolar organizer regions in exocrine pancreas in chronic pancreatitis and ductal adenocarcinoma. Diagnostic value and cytophysiologic correlate. 慢性胰腺炎和导管腺癌外分泌胰腺的核仁组织区。诊断价值与细胞生理相关。

General & diagnostic pathology Pub Date : 1997-02-01

A Pollok

{"title":"Nucleolar organizer regions in exocrine pancreas in chronic pancreatitis and ductal adenocarcinoma. Diagnostic value and cytophysiologic correlate.","authors":"A Pollok","doi":"","DOIUrl":"","url":null,"abstract":"The epithelium of small excretory ducts of the pancreas was investigated in sections obtained from 33 total-pancreatectomy-specimens by means of AgNOR-silver-staining as described by Ploton [26], in some modification. AgNOR-staining was evaluated by automatic image analysis. Specimens were split into four groups: mucoid transformation in chronic pancreatitis (11 cases), papillary hyperplasia in chronic pancreatitis (eight cases), ductal adenocarcinoma (14 cases) and control-group of pancreas-transplants (five cases). Parameters estimated for each cell are as follows: number of intranucleolar dots, number of extranucleolar dots, total number of dots, ratio of extra- and intranucleolar dots, total dot area, mean single dot area, nuclear area, ratio of total dot- and nuclear area, dot-density. Ratio of extra- and intranucleolar dots, ratio of total dot- and nuclear area as well as dot-density allowed differentiation between papillary hyperplasia in chronic pancreatitis, grade III, and ductal adenocarcinoma, grade I. A correlation was found between the number of extranucleolar dots and proliferative activity as well as between the number of intranucleolar dots and protein-synthesis activity of epithelial cells of small excretory ducts of the pancreas.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"199-209"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lethal complications in a case of sigmoid diverticulitis. A case report. 乙状结肠憩室炎致死性并发症1例。一份病例报告。

General & diagnostic pathology Pub Date : 1997-02-01

U Oehler, A Bulatko, H Jenss, B Helpap

{"title":"Lethal complications in a case of sigmoid diverticulitis. A case report.","authors":"U Oehler, A Bulatko, H Jenss, B Helpap","doi":"","DOIUrl":"","url":null,"abstract":"A 61-year-old female complaining of arthralgia was repeatedly treated with antibiotics and also with prednisolone. A chronic polyarthritis was assumed. In hospital, leukocytosis of 21.000 was found one day before death as well as moderate anemia. Colonoscopy was rejected by the patient. A computer tomography revealed destructive arthritis of the symphysis, vertebral osteochondrosis L5/S1, and sigmoid diverticulosis. The patient died with clinical signs of central dysregulation. At autopsy, a covered perforation of a sigmoid diverticulum with purulent peridiverticulitis was found. The 5th lumbal vertebra and the symphysis showed hematogenic abscesses. Microabscedating pneumonia, purulent meningitis and hypophysitis, and mycotic aneurysm of the basilar artery with lethal rupture were further results of hematogenic spread. Death was caused by massive subarachnoidal hemorrhage. This history is not untypical for elder patients with complicated diverticular disease. The intestinal perforation is often clinically occult due to only few and unspecific symptoms which cannot be exactly attributed to the colon. In the last ten years, we have found lethal complications of sigmoid diverticulitis at a frequency of 0.32% (5 cases in 1.557 subsequent autopsies). The clinical differential diagnosis included diverticulitis in none of the cases. This underlines the importance of autopsies for quality control in medicine, because modern diagnostic methods such as computer tomography were not able to give the correct diagnosis in these cases.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"231-4"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20021302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital microvillous atrophy: report of two cases. 先天性微绒毛萎缩2例报告。

General & diagnostic pathology Pub Date : 1997-02-01

H Steininger, R Behrens, G Faller, C Schindler, T Kirchner

引用次数: 0

Diffuse expansive cavernous hemangioma of the gallbladder. 胆囊弥漫性扩张性海绵状血管瘤。

General & diagnostic pathology Pub Date : 1997-02-01

M Mayorga, M Hernando, J F Val-Bernal

引用次数: 0

Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas. 基质金属蛋白酶和金属蛋白酶组织抑制剂在人软骨肉瘤中的免疫定位。

General & diagnostic pathology Pub Date : 1997-02-01

A Kawashima, Y Okada, I Nakanishi, Y Ueda, K Iwata, A Roessner

{"title":"Immunolocalization of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomas.","authors":"A Kawashima, Y Okada, I Nakanishi, Y Ueda, K Iwata, A Roessner","doi":"","DOIUrl":"","url":null,"abstract":"We have immunohistochemically examined the localization of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in human chondrosarcomas (CHS) (23 cases) and benign chondroid lesions (BCL) (16 cases of osteochondromas and 11 cases of enchondromas). In CHS, all the MMPs and TIMPs examined were positive. Among them, MMP-1 was immunolocalized in more than 90% of both CHS and BCL, but positive score of MMP-1 was significantly higher in CHS than that in BCL (p < 0.01). Compared with BCL, CHS expressed MMP-3 at a low level, and more often positive in MMP-9. It is possible that chondrosarcoma might have a tendency to lose the ability to secrete MMP-3, which is a metalloproteinase that can degrade cartilage proteoglycans and is related to normal cartilage turnover. MMP-2, TIMP-1 and TIMP-2 were immunolocalized in more than 70% of the cases of both BCL and CHS, but the positive scores of these were not statistically different between the two groups. Interestingly, in several cases of CHS, both MMP-1 and MMP-9 immunostains were observed preferentially within the cells at the marginal areas of cartilaginous lobules. These findings suggest that increased expression of MMP-1 and MMP-9 and decrease in MMP-3 expression are associated with the malignant phenotype of the cartilaginour tumors.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"129-37"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20022844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of P-glycoprotein in high grade osteosarcomas with special emphasis on chondroblastic subtype. p -糖蛋白在高级别骨肉瘤中的表达，特别强调成软骨细胞亚型。

General & diagnostic pathology Pub Date : 1997-02-01

K Radig, C Häckel, J Herting, Y Oda, U Mittler, W Neumann, A Roessner

{"title":"Expression of P-glycoprotein in high grade osteosarcomas with special emphasis on chondroblastic subtype.","authors":"K Radig, C Häckel, J Herting, Y Oda, U Mittler, W Neumann, A Roessner","doi":"","DOIUrl":"","url":null,"abstract":"The development of chemoresistance is one of the major clinical problems in the therapy of malignant bone tumors in childhood. The expression of membrane-bound P-glycoprotein turned out to be an essential factor in the evidence of resistant tumor cells. To investigate the significance of multidrug resistance in the prognosis of highly malignant osteosarcomas, the immunohistologic expression of P-glycoprotein was investigated in the tumor tissue of 52 patients under special consideration of the histologic subtype. The data were compared with the histologic regression grade in the resection specimen and correlated with clinical data. Formalin-fixed, paraffin-embedded tissue and, additionally, fresh frozen material taken from the primary biopsy were stained using monoclonal antibody JSB1. 29 (55%) of the tumors investigated were P-glycoprotein positive. Considering the response to chemotherapy, no conclusion could be drawn regarding P-glycoprotein expression, regression grade in the resection specimens, and the clinical follow-up. P-glycoprotein was detected in only 52% of the non-responders. A positive reaction was also evidenced in 59% of the patients with high chemosensitivity. A comparison of the histologic subtypes yielded a significant result in the chondroblastic osteosarcomas. 11 of 12 cases showed a strong expression of P-glycoprotein. Most of the cases were non-responders, and using Kaplan-Meier live tables, an unfavorable clinical outcome could be demonstrated. Possibly, chondroblastic tumors have a special position among osteosarcomas because of their differentiation.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"139-45"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal involvement in myeloproliferative and lymphoproliferative disorders. A study of autopsy cases. 骨髓增生性和淋巴增生性疾病的肾脏受累。尸检案例研究。

General & diagnostic pathology Pub Date : 1997-02-01

J C Xiao, R Walz-Mattmüller, P Ruck, H P Horny, E Kaiserling

{"title":"Renal involvement in myeloproliferative and lymphoproliferative disorders. A study of autopsy cases.","authors":"J C Xiao, R Walz-Mattmüller, P Ruck, H P Horny, E Kaiserling","doi":"","DOIUrl":"","url":null,"abstract":"A considerable proportion of cases of myeloproliferative and lymphoproliferative disorders exhibit renal involvement. However, it is unclear whether the cytologic features, immunophenotype or grade of malignancy of the cells infiltrating the kidney differ from those of the primary tumor. This study was performed on 120 autopsy cases with the following diagnoses: acute myelogenous leukemia (AML, n = 22; subtypes M1 + M2, n = 12, subtype M4, n = 10), chronic myelogenous leukemia (CML, n = 7), agnogenic myeloid metaplasia/myelofibrosis (AMM/MF, n = 6), acute lymphocytic leukemia (ALL, n = 6), chronic lymphocytic leukemia (CLL, n = 9), other low-grade non-Hodgkin's lymphomas (low-grade NHL, n = 24), high-grade NHL (n = 21) and multiple myeloma (MM, n = 25). Renal involvement was investigated by light microscopy and immunohistochemistry. It was found in 34% of the cases, and was most common in ALL (83%) and low-grade NHL (50%) and least common in high-grade NHL (10%) and MM (12%). Dense infiltration of almost the entire kidney was most commonly seen in AML, low-grade NHL and ALL. Infiltration was bilateral and involved both the cortex and medulla in the majority of cases. When involvement of other organs was compared with that of the kidney, the lung was found to be involved in approximately the same number of cases, but liver involvement was more common and heart involvement less common. Reactive lymphocytic infiltration of the kidney was found in 18 of the 120 cases (15%), and was distinguished from scanty tumorous infiltration by immunohistochemical staining. No major phenotypical differences were found between the tumor cells infiltrating the kidney and those of the primary tumors in the bone marrow or lymph nodes. However, in one case of CML, the cells infiltrating the kidney were negative for KP1 and chloroacetate esterase, but could be identified by reactivity for CD34. The grade of malignancy in NHL was similar in both the nodal and renal manifestations.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 3-4","pages":"147-53"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20020754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0