General & diagnostic pathology最新文献_第10页

Nuclear grade in invasive ductal breast carcinoma. Comparative study on nuclear grade and morphometric parameters, hormonal receptor status, flow cytometric analysis and lectin histochemistry. 浸润性导管性乳腺癌的核分级。核分级及形态学参数、激素受体状态、流式细胞分析及凝集素组织化学的比较研究。

General & diagnostic pathology Pub Date : 1996-10-01

N Jonjić, E Mustać, M Krstulja, G Zamolo, T Valković, F Sasso, C Bottin, M Melato

引用次数: 0

Adult solitary cutaneous myofibroma: a case report. 成人孤立性皮肤肌纤维瘤1例。

General & diagnostic pathology Pub Date : 1996-10-01

S Rahimi, M Santoni, A O Muda

引用次数: 0

DNA-aneuploidy as marker for neoplasia in G1-urothelial carcinomas. dna非整倍体作为g1 -尿路上皮癌肿瘤发生的标志。

General & diagnostic pathology Pub Date : 1996-10-01

B Planz, E Striepecke, J M Wolff, P Effert, G Jakse, A Böcking

引用次数: 0

Flow cytometric DNA-analysis of cells obtained with fine needle aspiration biopsy of the breast. 乳腺细针穿刺活检所得细胞的流式细胞术dna分析。

General & diagnostic pathology Pub Date : 1996-06-01

A Niezabitowski, B Lackowska, A Gruchala, J Ryś, A Jaszcz, W Szklarski, A Wasilewska, K Herman

{"title":"Flow cytometric DNA-analysis of cells obtained with fine needle aspiration biopsy of the breast.","authors":"A Niezabitowski, B Lackowska, A Gruchala, J Ryś, A Jaszcz, W Szklarski, A Wasilewska, K Herman","doi":"","DOIUrl":"","url":null,"abstract":"In 221 consecutive, unselected palpable breast lesions, evaluated by aspiration biopsy, flow cytometric DNA analysis was performed on cell material remaining in needle and syringe after smears had been prepared. DNA histograms were obtained in 186 cases, whereas in 44 samples the cell number was too low for detailed estimation of cell kinetics. Aneuploidy was observed in 71 of 90 samples where cancer cells were detected by cytology, in four of 20 samples where breast cancer could only be suspected and in 12 of those 76 where no cancer cells occurred in cytologic specimens. The average S-phase and S+G2M values were higher in aneuploid than in diploid cell samples. In the group of 40 cases (34 breast cancers, 6 benign lesions) comparative analysis of DNA histograms obtained on aspirates and tissue fragments revealed a concordance in 88.9%. Modified radical mastectomy was performed in 41 breast cancers and in 33 cases ductal infiltrating carcinomas were recognized by histology and scored according to Bloom-Richardson score. Cell features assessed by cytology in these cases did not correlate with histology. In most cases, however, nuclear polymorphism and numerous naked nuclei were present in cytologic specimens. These alterations, characterized as score 3, reflect values of DNA-index in compartment of 1.4-1.8.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 1","pages":"33-9"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19764151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of non-Hodgkin lymphoma of the maxilla with support of polymerase chain reaction. 聚合酶链反应在上颌骨非霍奇金淋巴瘤诊断中的应用。

General & diagnostic pathology Pub Date : 1996-06-01

H A Baba, L Tietze, S Merkelbach, L Füzesi

引用次数: 0

Morphologic distribution of transforming growth factor alpha in the gastrointestinal tract--its presence in neuroendocrine cell type of the colon mucosa. 转化生长因子α在胃肠道中的形态分布——在结肠粘膜神经内分泌细胞类型中的存在。

General & diagnostic pathology Pub Date : 1996-06-01

W Back, G Rohr

{"title":"Morphologic distribution of transforming growth factor alpha in the gastrointestinal tract--its presence in neuroendocrine cell type of the colon mucosa.","authors":"W Back, G Rohr","doi":"","DOIUrl":"","url":null,"abstract":"Among the growth factor proteins of the epidermal growth factor (EGF)-family especially the transforming growth factor alpha (TGF-alpha) and EGF can be detected in the mucosa of the gastrointestinal tract. In this study, immunohistochemistry on 15 different human tissue probes of gastrointestinal and colonic mucosa was carried out to visualize possible deposits for TGF-alpha in the mucosa and to compare different compartments of the upper and lower gastrointestinal epithelium. Most interestingly, in the colon there is a strong TGF-alpha-like immunoreactivity in neuroendocrine cells as evidenced by double labeling experiments with common neuroendocrine markers and by immunoelectron microscopy. In the upper gastrointestinal epithelium, however, all neuroendocrine cell types are negative for TGF-alpha, but parietal cells of the gastric corpus epithelium show strong cytoplasmic immunostaining for TGF-alpha. The neuroendocrine cells of the colon-harboring TGF-alpha-like immunoreactivity in their neurosecretory granules belong morphologically to the so-called L-cell type. Along with the well-known presence of EGF-receptor in the mucosa of the gastrointestinum, TGF-alpha, which is very likely to be released by this neuroendocrine cell type of the colon, acts in a paracrine mode. These findings represent the first morphologic indication that in the colonic epithelium growth regulation is under neuroendocrine control.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 1","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19764149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolated tumor cells in the bone marrow: value of a new diagnostic method in the TNM-staging of solid tumors. 骨髓分离肿瘤细胞:一种新的诊断方法在实体瘤tnm分期中的价值。

General & diagnostic pathology Pub Date : 1996-06-01

M Werner, A Nasarek, A Georgii

引用次数: 0

Cell proliferation in salivary gland tumors. 唾液腺肿瘤的细胞增殖。

General & diagnostic pathology Pub Date : 1996-06-01

A Skálová, I Leivo

{"title":"Cell proliferation in salivary gland tumors.","authors":"A Skálová, I Leivo","doi":"","DOIUrl":"","url":null,"abstract":"Salivary gland tumors often pose considerable difficulty in differential diagnostic and prognostic assessment based on histomorphologic grounds alone. Histomorphology may poorly correlate with clinical outcome and the tumors within the same type in classification schedule exhibit different clinical courses. Prognostic relevance of various cell proliferation markers has been investigated in many types of human cancer, recently including salivary gland tumors. Evaluation of DNA content by flow cytometry and by cytophotometry, AgNOR technique, and immunohistochemical detection of antigens in cycling cells such as the Ki67 antigen and proliferating cell nuclear antigen (PCNA) have been applied to a variety of benign and malignant salivary gland tumors in only few studies so far. Cell proliferation, assessed with the MIB1 antibody, that recognizes the Ki67 antigen in proliferating cells, represents a significant prognostic factor for acinic cell carcinomas and mucoepidermoid carcinomas of salivary gland origin. Moreover, much lower proliferative activity as assessed with the MIB1 antibody helps to distinguish difficult cases of polymorphous low grade adenocarcinomas from adenoid cystic carcinomas and may contribute to differentiation of solid myoepithelial cell-rich pleomorphic adenomas from various malignant tumors. Thus, assessment of cell proliferation in salivary gland tumors using the MIB1 antibody and PCNA in paraffin-embedded tissue should be incorporated into routine immunohistologic evaluation of histologically difficult cases of salivary gland tumors.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 1","pages":"7-16"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19764148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation spectrum of p53 gene in highly malignant human osteosarcomas. 高度恶性人骨肉瘤p53基因突变谱分析。

General & diagnostic pathology Pub Date : 1996-06-01

K Radig, R Schneider-Stock, Y Oda, W Neumann, U Mittler, A Roessner

引用次数: 0

Stereologic study of splenic tissue compartments from traumatically injured and cancer patients. 创伤性损伤及肿瘤患者脾组织腔室的体视学研究。

General & diagnostic pathology Pub Date : 1996-06-01

N M Milićević, A Cuschieri, A Xuereb, Z Milićević

{"title":"Stereologic study of splenic tissue compartments from traumatically injured and cancer patients.","authors":"N M Milićević, A Cuschieri, A Xuereb, Z Milićević","doi":"","DOIUrl":"","url":null,"abstract":"Carefully age-matched groups of patients, surgically treated for either traumatic injury (n = 5; mean = 71.2 years) or for early stage carcinoma (n = 5; mean = 74.2 years), were used to investigate all the splenic tissue compartments applying model-based stereology. The point-counting method was utilized to study the volume densities of following tissue compartments: red pulp, perifollicular zone, white pulp (this compartment was divided into two subcompartments: follicles and periarteriolar lymphatic sheath), marginal zone and connective tissue. The following stereologic parameters of lymphoid follicles were determined: areal numerical density (the number of follicles per mm2 of tissue section), the numerical density (number of follicles per mm3 of tissue), and the mean follicle diameter. The identity of tissue compartments was verified using immunohistochemical staining for B- and T- lymphocytes. Significantly increased volume densities of perifollicular zone and periarteriolar lymphatic sheath were registered in patients treated for traumatic spleen injury. On the other hand, the significantly increased number of lymphoid follicles per mm2 and per mm3 of splenic tissue were registered in the group of cancer patients. The differences observed, whether attributable to the immune stimulation of the ruptured spleen or the alterations of the immune system due to malignant disease, suggest that this issue deserves further attention.","PeriodicalId":79430,"journal":{"name":"General & diagnostic pathology","volume":"142 1","pages":"41-4"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19764152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0