DNA-aneuploidy as marker for neoplasia in G1-urothelial carcinomas.

General & diagnostic pathology Pub Date : 1996-10-01
B Planz, E Striepecke, J M Wolff, P Effert, G Jakse, A Böcking
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Abstract

Biopsies from 60 grade-1 urothelial carcinomas and 50 normal bladder mucosae have been investigated by DNA-image-cytometry after enzymatic cell separation to determine the diagnostic sensitivity of DNA-aneuploidy for the identification of low grade urothelial neoplasia. Two different modes to detect DNA-aneuploidy were compared: the conventional application of a threshold at 2.2c for the modal DNA value and the use of the Kolmogoroff-Smirnow (KS) test to compare GO/1 phase fractions of the cells under analysis and the internal reference cells. Whereas the specificity for both types of analysis was 100%, the sentitivity of the latter method was 73.3% compared to 48.3% of the conventional procedure. Therefore, the application of the KS-test for the identification of DNA- stemlines as aneuploid results in an increased detection rate was compared with the conventional threshold procedure. The high prevalence of DNA- stemline aneuploidy even in low grade urothelial cancers may qualify this marker as a possible aid in the cytologic evaluation of urine and bladder washings.

dna非整倍体作为g1 -尿路上皮癌肿瘤发生的标志。
我们对60例1级尿路上皮癌和50例正常膀胱粘膜的活检进行了酶解细胞分离后的dna图像细胞术研究,以确定dna非整倍体对鉴别低级别尿路上皮肿瘤的诊断敏感性。比较了检测DNA非整倍体的两种不同模式:传统的DNA模态值阈值为2.2c,以及使用Kolmogoroff-Smirnow (KS)测试来比较被分析细胞和内参细胞的GO/1相分数。两种分析方法的特异性均为100%,后一种方法的敏感性为73.3%,而传统方法的敏感性为48.3%。因此,与传统的阈值程序相比,应用ks测试鉴定DNA-茎系为非整倍体的结果增加了检出率。即使在低级别尿路上皮癌中,DNA-茎系非整倍体的高流行率可能使该标记物有资格作为尿液和膀胱清洗细胞学评估的可能辅助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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