Romanian journal of physiology : physiological sciences最新文献

{"title":"Morpho-functional aspects of the influence of procaine and diethylaminoethanol treatment on the immune system of rabbits.","authors":"M Bordea,&nbsp;C Ardeleanu,&nbsp;A Dolganiuc,&nbsp;A Olinescu,&nbsp;A Vrăbiescu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Research was conducted in 24 male rabbits divided into three equal groups: 1) Controls; 2) Given daily i.m. injections with 15 mg/kg body weight of procaine for 30 days; 3) Given daily i.m. injections with 15 mg/kg body weight of diethylaminoethanol (DEAE) for 35 days. Blood samples were taken from the auricular vein to perform haematological investigations: before treatment, during treatment and for another 7 weeks after termination of treatment. At the end of the experiment, the animals were sacrificed in order to collect: 1) leukocytes, macrophages and lymphocytes from their spleen, for the purpose of immunological investigations, and 2) fragments of bone marrow (femur), thymus and spleen, for histopathological investigations. The result of haematological investigations indicated a statistically significant increase of circulating lymphocytes had occurred in rabbits treated with DEAE. There was an increase of percentage as well as of number of circulating lymphocytes. The increase occurred gradually in the course of treatment and continued after treatment was terminated. There were higher values than the initial ones by 44.4% and 66.4%, respectively, during the second and the fourth week after termination of treatment. Afterwards, the values started decreasing, so that by the end of the experiment (week 7 after termination), the values came close to those before treatment. Immunological findings indicated that T lymphocytes collected from rabbits treated with procaine or DEAE did not synthesize migration inhibition factors. Procaine and DEAE were found, in vitro, not to have a polyclonal stimulating effect over T lymphocytes. A study of blastic differentiation showed lymphocytes from treated rabbits to incorporate a larger amount of tritiated thymidine, by 52.6% in the procaine group and by 90% in the DEAE group, than those from non-treated rabbits. Histopathological examination indicated signs of a more intense activity in tissue formations involved in cell proliferation in the lymphoid organs collected from DEAE treated rabbits.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"111-26"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary EEG study of protective effects of Tebonin in transient global cerebral ischemia in rats.","authors":"L Zăgrean,&nbsp;R Vătăşescu,&nbsp;A M Munteanu,&nbsp;M Moldovan,&nbsp;D A Niţă,&nbsp;M Coculescu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Free radicals and platelet activating factor (PAF) have been implicated as important mediators in neuronal injury after cerebral ischemia-reperfusion and, particularly, in postischemic hypoperfusion. The electroencephalography (EEG) is a real time reflection of changes in neuronal activity and metabolism. The objective of this study was to investigate the effects of preventive treatment with Ginkgo biloba extract (EGb 761--Tebonin) in cerebral global ischemia and reperfusion in rats using computerized EEG analysis. Ginkgo biloba extract, known to be, in vitro, a free radicals scavanger and a PAF--antagonist, was administrated in dose of 100 mg/kg over 24 hours, for 5 days before and 5 days after cerebral ischemia--reperfusion. The apparition of isoelectric EEG (flat-line) following 4-vessel occlusion was observed after a mean time of 25 sec. in Ginkgo biloba treated rats and after 18 sec. in control rats (p < 0.0015). Computerized spectral analysis of EEG has shown that the percentage of slow waves at 10 minutes after reperfusion was 117% higher in control group than in Ginkgo biloba group (p < 0.015) and the percentage of slow waves after 15 minutes of reperfusion was 100% higher in the control group than in the Ginkgo biloba group (p < 0.02). Five days after cerebral ischemia--reperfusion the percentage of slow waves was unsignificantly higher in the control group than in the Ginkgo biloba group (p > 0.05).</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"161-8"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The quantification of intrahemispheric EEG asymmetry in rat.","authors":"M Moldovan,&nbsp;A M Munteanu,&nbsp;D A Niţă,&nbsp;D P Popa,&nbsp;L Zăgrean","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have developed a simple model to quantify the intrahemispheric asymmetries (IA) present in the background EEG activity (BEA), of the adult rat during restrained wakefulness and anesthesia with Ether and chloral hydrate (CHL). We used a collectivity of 40 adult (140 g) male Wistar rats. The epidural EEG activity, was quantified within the 1-30 Hz band by six numerical parameters: root mean square (RMS), mean spectral frequency (MSF), spectral edge frequency at 95% (SEF95) and the relative spectral power (RSP) within the Theta (4.5-7.5 Hz), Alpha (7.5-12 Hz) and Beta (12.5-30 Hz) bands. A significant IA was considerate when simultaneously present in both hemispheres at p (t-Test) < 0.05. The only IA detected in the BEA of the awake restrained rat was the increased theta RSP over the fronto-parietal areas. The theta asymmetry was abolished under both types of anaesthesia. While ether had a global effect on BEA, CHL induced a strong IA reflected in all parameters except RMS and Theta RSP. The fronto-parietal areas seem to be relatively resistant to CHL. It is discussed that the intrahemispheric theta dominance over the frontoparietal areas should be regarded as an indicator of alertness in rats.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"33-9"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of lithium in reversing hydroxyurea induced suppression of hematopoietic progenitor cells in vitro using retroviral infected long-term marrow cultures.","authors":"O R Oakley,&nbsp;N K Hughes,&nbsp;J D Phillips,&nbsp;N J Birch,&nbsp;V S Gallicchio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lithium has been known for its ability to induce the production of hematopoietic cells following administration in vivo to minimize the toxic effects on hematopoiesis as a consequence of drug treatment. The drug hydroxyurea (HU), a ribonucleotide reductase inhibitor, has been used in the treatment of a variety of neoplastic and non-neoplastic diseases, such as cancer and sickle cell anaemia. Hydroxyurea has more recently been implicated for use in the treatment of acquired immunodeficiency syndrome (AIDS). However, its major limitations have been due to its toxicity. Hydroxyurea selectively inhibits DNA synthesis and due to its brief duration, the drug is only toxic to those cells which are selectively synthesizing DNA during the period of exposure. The most important of these toxicities, and which serves as a dose limiting factor in treatment, is the induction of bone marrow suppression. In this study we investigated the possible beneficial effects of administering lithium (LiCl) to murine leukemia virus (MuLV) infected and non-infected long term bone marrow cultures (LTBMC). These cultures were then treated with either 0.2 mM hydroxyurea, 1.0 mM LiCl, or a combination of both. Samples were collected from LTBMC supernatants at 1, 2, 3, 4, 5 and 6 weeks post-treatment. Culture supernatants were then monitored to observe their repopulation of hematopoietic progenitors. The results demonstrated the effects of lithium in restoring hydroxyurea suppressed numbers of myeloid (CFU-GM) progenitors to within a normal range and also in re-establishing erythroid (BFU-E) progenitors.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"67-78"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diethylaminoethanol action in the arthritis with Freund adjuvant, in rats.","authors":"A Vrăbiescu,&nbsp;T Poli,&nbsp;G Coman,&nbsp;F Dolcoş,&nbsp;C Găinaru,&nbsp;C Carazanu,&nbsp;G Ciobanu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors have studied the action of diethylaminoethanol on Freund adjuvant arthritis, induced in female Lewis rats. They worked on 3 groups, each one including 14 rats, weighing 110-130 g: group I = control; group II = rats injected intracutaneous with 0.1 ml Freund adjuvant; group III = rats injected with Freund adjuvant and treated with diethylaminoethanol i.m. (10 mg/kg body weight), and gel application (2.5%) on paws and tail, daily. During the experiment clinical observations and measurements were made and when the animals were killed, blood was sampled for haematological and immunological assays (CD4, CD8, CD25 T cells and NK cells, antinuclear autoantibody and immune complexes). While in all the rats from group II (without treatment) inflammatory processes developed at the level of the peripheral joints, in group III (diethylaminoethanol treated), these ones were present in only 64% of the rats and by much more reduced forms, followed by a short period of involution. The paw volume, measured with an electronic plethysmometer, was more reduced in the treated rats (7.1-14.2%) than in the non treated ones (27.7-29.3%). The haematologic examination showed an increased number of neutrophiles in both groups with FA injecting. The immunological investigations revealed: a decrease of CD4 cells and an increase of CD8 T cells and NK cells in both groups, a much more decreased level (13.2%) of circulatory immune complexes in treated rats, as compared to the non-treated ones (71.7%). No differences were found regarding the CD25 cells and antinuclear antibodies. The histo-pathological examination showed that the intensity and the extension area of the joint lesions (granulation tissue with fibrous change, cartilage invasion and dilaceration, bone atrophy) were much more reduced in the treated rats. The authors put forward the hypothesis that these effects might be due to diethylaminoethanol antiinflammatory properties.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"99-110"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complementary study of boron absorption by coffee leaves using the neutron-capture-radiography method.","authors":"L G Meneses,&nbsp;R J Dam,&nbsp;M Badilla","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Young coffee plants were treated by putting a droplet of a solution containing 10B-enriched boron on some of their leaves. The rate of boron absorption by leaves (measured using neutron capture radiography) was found to be in the range of 1.9 to 5.3 nmol boron per h. This means that it requires several days for a coffee leaf to absorb most of the boron supplied by foliar spray in the usual agronomic treatments. Technically, the use of the method of neutron capture radiography has revealed to be quite sensitive and applicable to liquid micro-samples (a few microL in volume) even with the low-intensity neutron fluxes available in Costa Rica.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"169-77"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood levels of some electrolytes and hormones during exercise in athletes.","authors":"E Duma,&nbsp;P Orbai,&nbsp;P Derevenco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Athletes (8 trained medium and long distance runners) have been explored before and after physical exercise under two testing procedures. After the ergometric test significant increases of Ca++ and Mg++ in serum and significant decrease of Mg++ in erythrocytes occurred. A marked increment of the cortisol level and lesser changes of the thyroid hormones have been found. After the specific training program, apart from the significant reduction of the erythrocyte Mg++, a very significant increase of the cortisol level and large increments of T3 occurred. All subjects showed good coping resources, with inter-individual differences for other three psychological variables.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of magnesium sulfate on learning, emotivity and spontaneous motility in rats.","authors":"V Lupu,&nbsp;M Dorofteiu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study was performed on three groups, each including 10 male Wistar rats, weighing 110-130 g: group 1, the control group; group 2, animals treated with magnesium sulfate in a dose of 100 mg/kg body/day i.m.; group 3, animals treated with double dose of magnesium sulfate (200 mg/kg body/day i.m.). All animals were tested for emotivity and spontaneous motility in the open field, as well as for their capacity of acquiring active avoidance conditioned reflexes in the shuttle-box, for 9 days. Magnesium sulfate decreased significantly the learning ability, decreased emotivity scores regardless of the administered dose, and increased significantly spontaneous motility scores only in the double dose treated group.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"179-85"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21833361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diurnal behaviour of some salivary parameters in patients with diabetes mellitus (protein concentration, amylase activity, density)--note I.","authors":"M Artino,&nbsp;M Dragomir,&nbsp;S Ionescu,&nbsp;D Bădiţa,&nbsp;V Niţă,&nbsp;E Chiţoi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study was carried out on 31 diabetic patients of both sexes hospitalised in the Clinical Hospital of Diabetes and Nutritional Diseases \"Dr. I. Cantacuzino\". They were divided in 2 groups: patients with insulin-dependent diabetes (IDDM) and noninsulin-dependent diabetes (NIDDM) and compared with a group of control non-diabetic subjects. Total mixed saliva was sampled in the morning (07:30-08:00) and in the afternoon (17:30-18:00) without stimulation and tested for amylase activity, proteins and density. The study revealed that the density and the amylase activity have the same diurnal behaviour for the diabetic patients and healthy control subjects either. The only difference was the amplitude of maximal activity (in the morning for density and in the afternoon for the amylase activity). Salivary proteins have a similar diurnal behaviour for both diabetic groups (maximal values in the morning and minimal values in the afternoon) in contrast to healthy control subjects that presented less obvious diurnal changes.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"79-84"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-2 alterations in the immune status of patients with mammary neoplasm.","authors":"A Blidaru,&nbsp;C I Bordea,&nbsp;C G Viişoreanu,&nbsp;M Bordea,&nbsp;I Iliescu,&nbsp;D Duţescu,&nbsp;F Radu,&nbsp;H Drăgoescu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immune control is achieved by the balance between tumour reduction mechanisms and the \"immunological creeping\" phenomenon. The purpose of this study was to look into the alterations of the immune effector component in the case of mammary tumours. The study included 42 cases of women with a clinical diagnosis of mammary tumour, initially referred for surgery, with a mean age of 45.07 (18-65 years of age). Neither radiotherapy nor chemotherapy had been given to the patients before surgery. Histopathological examination performed intraoperatively as well as later, under paraffin, revealed 12 cases of malign tumours. The control group consisted of 18 women (blood donors), apparently healthy, with a mean age of 41 (30-49 years of age). In both groups, the immune status was assessed by means of: lymphocyte counts, T-lymphocyte counts (TL), rosetting, lymphocyte blastic transformation test (BLT) under the action of polyclonal mitogens, immunoglobulin dosing and Interleukin-2 (IL-2) determination in lymphocyte cultures stimulated with mitogens. The conclusion of our results has been that even in the less advanced stages of mammary cancer, there occur alterations of the immune response represented by: A decrease in the total number of lymphocytes, especially through a decrease of the TL number; A decrease of the lymphocyte blastic transformation index on stimulation with PHA; A decrease of IL-2 concentration in cultures stimulated with mitogens through the impairment of the TL CD4+ (TL Helper) function. The decrease of IL-2 production capacity influences the transmission of the immune message to the cytotoxic TL and thus impairs the cytotoxic effector component whose role is to remove tumoral cells. Evidence of IL-2 involvement in the immunodeficiency of mammary cancer patients could prove useful for diagnosis purposes and could plead in favour of the therapeutic potential of this interleukin.</p>","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"35 1-2","pages":"127-34"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21834265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}