Romanian journal of physiology : physiological sciences最新文献

The interaction between the cholinergic and dopaminergic system in learning and memory process in rats. 大鼠学习记忆过程中胆碱能与多巴胺能系统的相互作用。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

V Hefco, K Yamada, Andreea Hefco, L Hritcu, A Tiron, T Nabeshima

{"title":"The interaction between the cholinergic and dopaminergic system in learning and memory process in rats.","authors":"V Hefco, K Yamada, Andreea Hefco, L Hritcu, A Tiron, T Nabeshima","doi":"","DOIUrl":"","url":null,"abstract":"In normal rats, muscarinic acetylcholine receptors (mAChRs) have a facilitating role on both short-term and long-term memory tested by Y-maze task and multi-trial passive avoidance test, respectively, since scopolamine, a specific mAChRs antagonist, impairs both types of memory. A low dose of nicotine (0.3 mg/kg b.w., i.p.), a specific nicotinic acetylcholine receptors (nAChRs) agonist, administered once caused a significant facilitating effect on short-term memory. A higher dose of nicotine (3 mg/kg b.w., i.p.) administered 5 consecutively days had about the same facilitating effect on short- and long-term memory without affecting information acquisition. In rats, having mAChRs and nAChRs blocked by means of scopolamine and chlorisondamine respectively, a low dose of nicotine administered once caused a significant improvement of long-term memory deficits without affecting significantly short-term memory. A higher dose of nicotine administered 5 consecutive days in rats with a double blockade of cholinergic receptors had the same ameliorating effect on long-term memory deficits as low dose. Our data suggest that the antiamnesic effect of nicotine can result from an action at nicotinic receptors subtypes not blocked by chlorisondamine or at nonnicotinic receptors.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25161040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of the muscular contractility of the vena porta in trypsinemia. 胰蛋白酶血症中门静脉肌肉收缩性的改变。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

A Saulea, V Ojog

引用次数: 0

New technical approaches in stereotaxic catheterization of cerebral ventriculi: implications for the L-arginine/NO synthase/nitric oxide cascade. 脑室立体定向置管的新技术途径:对l -精氨酸/NO合成酶/一氧化氮级联的影响。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

G Manolidis, C Neamţu, D Vasincu, Irina-Maria Jaba, Oana Rădăşanu, O C Mungiu

{"title":"New technical approaches in stereotaxic catheterization of cerebral ventriculi: implications for the L-arginine/NO synthase/nitric oxide cascade.","authors":"G Manolidis, C Neamţu, D Vasincu, Irina-Maria Jaba, Oana Rădăşanu, O C Mungiu","doi":"","DOIUrl":"","url":null,"abstract":"In order to study the actions of certain substances at cerebral level, a stereotactic device for ensuring a precise catheterization of points in certain cerebral areas was used. For the operation technique was used a stereotaxic atlas specifically designed for rat brain (G. Paxinos, C. Watson, 1998), which offers all the necessary information for the identification of the trepanation. Stereotaxic implantation of cannules in the brain is useful for microinjecting solutions containing various substances (in amounts of microl), directly and targeted in the anatomical structures of the brain. The technique described can use either metalic or silastic cannules, that have variable lumen (usually for adapting a Hamilton syringe). The cannules can be implanted at cerebroventricular level, having the possibility to target all the cerebral ventricles. The intracerebroventricular (icv) administration of L-arginine induces a significant increase of response latency for mechano-algesic test. The most obvious changes are induced following the administration of the association of L-NAME with L-arginine, situation when is manifested an important increase of the response latency, starting with 5 minutes post-administration and continuing up to 45 minutes determination. The increase is significantly higher compared with the results obtained with L-arginine alone. A similar evolution is registered in the case of the plantar test.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"83-9"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24871017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the cerebral vascular Doppler velocimetry and EEG in a group of 18 cases of Alzheimer's disease. 18例阿尔茨海默病脑血管多普勒测速和脑电图的研究。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

Simona I Gusti, Mugurel I Coteanu, Alice S Gusti

{"title":"Study of the cerebral vascular Doppler velocimetry and EEG in a group of 18 cases of Alzheimer's disease.","authors":"Simona I Gusti, Mugurel I Coteanu, Alice S Gusti","doi":"","DOIUrl":"","url":null,"abstract":"The authors studied the cerebral irrigation on a group of 18 patients with Alzheimer disease (60-70 year old) using the Doppler (D) ultrasound vascular method and EEG. They used a type UDP-10 Sonopan, with a sound acquisition system and graphic of recording on a 6 NEK-4 polyrecorder for registration of the carotid D curves together with ECG and phonocardiogram. EEG was registered using a Bioscript 2000. The statistical analysis of the D curves parameters for a group of 18 patients, compared to the parameters of a group of 40 clinically healthy persons, showed a decrease of the systolic speed with 24% and of the diastolic speed with 11%, a delay of the flow with the systolic spike in plateau and a murmur at the carotid arteries in 33% of the patients. These modifications point to the reduction of the cerebral vascular filling, determined especially by the atherosclerosis process which causes important narrowing of the vascular lumen but probably determined by Alzheimer disease also. We remarked the presence of theta waves in 42% of the patients and a small correlation (r = 0.38) with the decrease of the systolic speed in the test group.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"91-7"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24871016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between respiratory sinus arrhythmia and heart rate during anesthesia in rat. 麻醉大鼠呼吸窦性心律失常与心率的关系。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

M Moldovan, S Spulber, V Saravan, R Iosifescu, Ana-Maria Zăgrean, L Zăgrean

{"title":"The relationship between respiratory sinus arrhythmia and heart rate during anesthesia in rat.","authors":"M Moldovan, S Spulber, V Saravan, R Iosifescu, Ana-Maria Zăgrean, L Zăgrean","doi":"","DOIUrl":"","url":null,"abstract":"During inspiration the heart rate (HR) increases and during expiration it decreases. Contribution of respiratory sinus arrhythmia (RSA) to spontaneous heart rate variability (HRV) can be measured as the high frequency (HF) component of variation in consecutive R-R intervals on ECG. In conscious rats, slowing of HR is associated with an increase in HF. The aim of this study was to investigate whether this relationship between HF and HR is preserved during anesthesia in rat. A 15 minutes long ECG signal was recorded from rats (N=15) under moderate chloral hydrate (CHL) anesthesia. Recordings were extended with 45 minutes to investigate the effect of atropine (N=3), against controls (N=3). Short term HRV was investigated in 30 seconds long epochs. HF was considered the frequency band between 0.8 and 1.6 Hz. RSA was quantified as the relative spectral power of the HF. Respiratory frequency (RF) was quantified as the mean spectral frequency within the HF band. One minute estimates of HR, RSA and HF were calculated by averaging 3 epochs of 30 seconds overlapped 50%. The average HR was 427 +/- 3 bpm. The magnitude of RSA was 45 +/- 1% at a RF of 71 +/- 1 rpm. We found that: (1) the decrease in HR that occurs during CHL anesthesia in rat correlates with an increase in RSA; (2) atropine reduces RSA and the time-dependent decrease in HR; (3) the time-dependent increase in RSA is preserved after atropine. We conclude that the correlation between RSA and HR reflects the cardio-pulmonary coupling under parasympathetic control.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"31-9"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25161041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are polyamines involved in the contractile effects of angiotensin II in the rat aorta? (Polyamines and angiotensin II in rat aorta). 多胺是否参与大鼠主动脉血管紧张素II的收缩作用?(大鼠主动脉多胺和血管紧张素II)。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

Natalia Costuleanu, Liliana Foia, Simona Mihaela Slătineanu, M Costuleanu, G Petrescu

{"title":"Are polyamines involved in the contractile effects of angiotensin II in the rat aorta? (Polyamines and angiotensin II in rat aorta).","authors":"Natalia Costuleanu, Liliana Foia, Simona Mihaela Slătineanu, M Costuleanu, G Petrescu","doi":"","DOIUrl":"","url":null,"abstract":"Angiotensin II (AII) is a central factor involved in the pathophysiology of arterial hypertension and atherosclerosis. On the other hand, polyamines represent a family of organic cations with low molecular weight, playing intracellular regulatory roles essential for the cellular growth and differentiation. The cellular contents, the synthesis and the transport of polyamines are increased following the actions of AII, as well as of other cellular growth factors. Our results show that the administration of polyamines as pre-treatment modulates the contractile effects of extracellular AII (80 nM). This modulation is concentration-dependent and dual: the lower concentrations amplify and the higher concentrations reduce the effects of AII in the isolated rat aorta rings without endothelium. Moreover, DL-alpha-Difluoromethylomithine (DFMO), a specific inhibitor of ornithine decarboxylase, does not significantly modify the contractile effects of AII. Thus, these data suggest that polyamines generated through this metabolic pathway are not involved in the contractile effects of AII in rat aortic vascular smooth muscle.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25163005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motor cortex plasticity--from physiology to clinical neurology. 运动皮层可塑性——从生理学到临床神经学。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

Cătălina Roxana Bohotin, Magda Bădescu, D N Popescu, V Bohotin

引用次数: 0

Pathogenic pathways in acute myeloid leukemias. 急性髓性白血病的致病途径。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

A Cucuianu

{"title":"Pathogenic pathways in acute myeloid leukemias.","authors":"A Cucuianu","doi":"","DOIUrl":"","url":null,"abstract":"Despite the common clinical, hematological and prognostic features that define acute myeloid leukemia (AML) there is considerable heterogeneity among individual cases, suggesting different pathogenic pathways. Based on a simple theoretical model, according to the vital characteristics of the leukemic clone (proliferative rate and resistance to apoptosis) we propose a classification of AML into two broad categories: a) high leukemic clone vitality (HLV) AML, corresponding roughly to the World Health Organization (WHO) classification group of entities \"AML with recurrent cytogenetic abnormalities\" and b) low leukemic clone vitality (LLV) or \"opportunistic\" AML corresponding to the WHO groups \"AML with multilineage dysplasia\" and \"therapy-related AML\". HLV-AML leukemic clones are characterized by rate-limiting genomic mutations capable of conferring proliferation/survival advantage over a normal hematopoietic environment while in LLV-AML, the leukemic clones are not particularly proliferative or apoptosis-resistant, but are nevertheless selected against an impaired, previously damaged hematopoietic environment. Such a pathogenesis-oriented classification might have therapeutic and prognostic implications, providing a theoretical basis for a further adaptation of the current standard treatment strategies to the individual characteristics of the AML patients.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"109-18"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24871019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental data regarding the implications of certain minimum structure enkephalin-like peptides in nociceptive processing. 某些最小结构脑啡肽样肽在伤害性加工中的意义的实验数据。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

Irina M Jaba, D Vasincu, G Manolidis, I Haulică, O C Mungiu

{"title":"Experimental data regarding the implications of certain minimum structure enkephalin-like peptides in nociceptive processing.","authors":"Irina M Jaba, D Vasincu, G Manolidis, I Haulică, O C Mungiu","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to investigate the importance of the amino acidic sequence at N-terminal end of certain minimum structure enkephalin-like peptides for the analgesic activity. Different groups of mice or rats were treated with 1) L-tyrosine (i.p. 200 mg/kg), 2) Tyr-Phe (i.t. 0.5 mg/rat), 3) Tyr-Pro-Phe (i.t. 0.5 mg/rat), 4) Gly-Tyr (i.t. 0.5 mg/rat), 5) Tyr-Gly-Gly (i.t. 0.5 mg/rat). Different tests were utilized to evaluate the antinociceptive effect of the substances tested: thermal nociception (hot plate test, plantar test), mechanical nociception (analgesymeter test). Tyr-Pro-Phe, Tyr-Gly-Gly, Tyr-Phe, but not Gly-Tyr, elicited analgesic activity. So, the presumption made in the case of atypical opioid peptides that opioid-like activity in case of peptides presumes a tyrosine residue at the N-terminal sequence, applies for shorter peptides. It appears also that minimal structure brain peptides with an N-terminal Tyr-Pro, rather than the Tyr-Gly-Gly-Phe sequence typical of other endogenous opioids, can provide better affinity for the opioid receptors and stronger analgesic activity. The inhibition of their analgesic effect by previous administration of naloxone proves that this effect is mediated through the endogenous opioid system.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"119-26"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24871020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling of ion permeation in K+ channels by nonequilibrium molecular dynamics simulations: I. Permeation energetics and structure stability. 离子在K+通道中渗透的非平衡分子动力学模拟:1 .渗透能量学和结构稳定性。

Romanian journal of physiology : physiological sciences Pub Date : 2004-01-01

A Neamţu, Daniela Suciu

{"title":"Modelling of ion permeation in K+ channels by nonequilibrium molecular dynamics simulations: I. Permeation energetics and structure stability.","authors":"A Neamţu, Daniela Suciu","doi":"","DOIUrl":"","url":null,"abstract":"Because of the great importance of physiological and pathophysiological processes in which ion channels are involved and because their operation is described by physicochemical laws, there have been many attempts to develop physical models able to describe the membrane permeability and also the structural and functional properties of the channel protein structures. In this study (in two parts) we present a series of simulations on a K+ channel model (KcsA) using Nonequilibrium Molecular Dynamics simulations (NEMD), in order to follow structure stability, permeation energetics and the possibility of obtaining quantitative information about the permeation process using the Linear Response Theory (LRT). On K+ ions were applied external forces to determine them to pass through the channel in a relatively small amount of time, accessible computationally. We ascertained a high resistance of the protein to deformation even in conditions when great forces were applied on ions (the system was far from equilibrium). The estimation of energy profiles in the course of ions passage through the channel demonstrates that these proteins create a conductivity pathway with no energetic barriers for ions movement across the channel (which could be present due to ions dehydration). The dynamic model used demonstrates (as proposed before in the literature after the examination of the static KcsA structure obtained by X-Ray crystallography) that this is due to the interaction of ions with the negatively charged carbonyl oxygens of the main polypeptide chain in the selectivity filter region.","PeriodicalId":79373,"journal":{"name":"Romanian journal of physiology : physiological sciences","volume":"41 1-2","pages":"69-82"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24871015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0