International journal of biological research in pregnancy最新文献_第8页

Multidrug resistant Salmonella sp isolated from chicken 从鸡中分离出耐多药沙门氏菌

International journal of biological research in pregnancy Pub Date : 2016-04-29 DOI: 10.14419/IJBR.V4I1.6030

A. Victor

{"title":"Multidrug resistant Salmonella sp isolated from chicken","authors":"A. Victor","doi":"10.14419/IJBR.V4I1.6030","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.6030","url":null,"abstract":"Antibiotic resistance has been a major problem in combatting Salmonella in poultry, this research was designed to determine the antibiotic resistance level of Salmonella isolated from freshly dead chicken. A total of 107 freshly dead chicken were collected from 23 different farms, birds were necropsied, liver and trachea were collected, Salmonella were recovered from the samples using peptone water, Rappaport-Vassiliadis R10 Broth and Salmonella-Shigella agar. Pure culture were identified using cultural, morphological and biochemical characteristics. The pure isolates were subjected to antibiotic test using disc diffusion method. Sixteen isolates of Salmonella were recovered, 3 of which were from the trachea while 13 were recovered from the liver. All of the Salmonella isolates were resistant to Amoxicillin 100% while Nitrofuratoin was least resisted with only 37.5% of the Salmonella isolates showing resistance. The antibiotic resistant pattern often observed in this study were AMP, AUG, TLY with 75% (12); AMP, AUG, TLY, CPR, ENR, 50% (8); while 6.3% (1) was resistant to all of the antibiotics tested. This result showed that there is an emergence of multi-resistance Salmonella in poultry, therefore it is important to carry out sensitivity test before administration of antibiotics in order to control poultry salmonellosis.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"34 1","pages":"64-66"},"PeriodicalIF":0.0,"publicationDate":"2016-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87976816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The effects of NACL, KCL and MGCL2 on the germination of Brassica rapa var. parachinensis seed NACL、KCL和MGCL2对辣白菜种子萌发的影响

International journal of biological research in pregnancy Pub Date : 2016-04-19 DOI: 10.14419/IJBR.V4I1.5854

M. Ghanad, R. Nulit, R. Go, Christina Yong Seok Yien

引用次数: 4

Response surface methodology for optimal immobilization of Aspergillus niger ATCC 1015 lipase by adsorption method 吸附法固定化黑曲霉ATCC 1015脂肪酶的响应面法研究

International journal of biological research in pregnancy Pub Date : 2016-04-19 DOI: 10.14419/IJBR.V4I1.6008

M. Osho, T. Popoola, T. Adeleye, C. Adetunji

引用次数: 5

Isolation, characterization and decolorization of textile dyes by fungal isolates from textile effluents 从纺织废水中分离真菌对纺织染料的分离、表征和脱色

International journal of biological research in pregnancy Pub Date : 2016-04-15 DOI: 10.14419/IJBR.V4I1.5967

Femi-Ola Titilayo, A. Emmanuel, A. Victor

{"title":"Isolation, characterization and decolorization of textile dyes by fungal isolates from textile effluents","authors":"Femi-Ola Titilayo, A. Emmanuel, A. Victor","doi":"10.14419/IJBR.V4I1.5967","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5967","url":null,"abstract":"Synthetic dyes have an adverse effect on the aquatic ecosystem and their toxic component has to be removed from the effluent before their exoneration. Microorganisms have the potentials to degrade complex and recalcitrant organic compounds into simpler fragments achieving complete mineralization. Thus, the aim of this work is to isolate and investigate the degradative potential of fungal isolates recovered from textile effluents. The fungal isolates encountered includes; Aspergillus niger (14.3 %), Aspergillus fumigatus (14.3 %), Cryptococcus sp. (28.6 %) and Candida albican (42.9 %). The fungal isolates utilized some selected synthetic dyes (Malachite green, Methylene blue, Eosin Y and Carbol fuchsine) as sole source of carbon and nitrogen. All the fungal isolates exhibited degradative and decolourization potential against the different dyes tested at varying degree. Highest decolourization (79.4%) was obtained by Aspergillus niger for Eosin Y dye, followed by Cryptococcus sp . (77.0%) for the same dye after five days incubation. However the least decolourization efficiency (35%) was obtained by Aspergillus fumigatus for Malachite green. The results have demonstrated the potential of the fungal isolates for the treatment of dye wastewater.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"94 1","pages":"46-51"},"PeriodicalIF":0.0,"publicationDate":"2016-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85698322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Influence of temperature and different culture media on growth of fusarium udum (Butler), causal organism of wilt of pigeonpea 温度和不同培养基对鸽子豌豆枯萎病病原菌枯萎菌生长的影响

International journal of biological research in pregnancy Pub Date : 2016-04-06 DOI: 10.14419/IJBR.V4I1.5901

Udaysingh Desai, Y. Andoji, S. Kamble

引用次数: 3

Phytochemical composition, in vitro antioxidant activity and acute toxicity of Irvingia gabonensis (O’Rorke) baill ethanolic leaf extract 加蓬草包乙醇叶提取物的植物化学成分、体外抗氧化活性及急性毒性研究

International journal of biological research in pregnancy Pub Date : 2016-03-24 DOI: 10.14419/IJBR.V4I1.5939

E. Ewere, Emmanuel Uka, Usunomena Usunobun

{"title":"Phytochemical composition, in vitro antioxidant activity and acute toxicity of Irvingia gabonensis (O’Rorke) baill ethanolic leaf extract","authors":"E. Ewere, Emmanuel Uka, Usunomena Usunobun","doi":"10.14419/IJBR.V4I1.5939","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5939","url":null,"abstract":"This study was aimed at determining the phytochemical composition, antioxidant effect and acute toxicity of Irvingia gabonensis (O’Rorke) baill (IG) ethanolic leaf extract. Qualitative phytochemcal analysis was carried out on the ethanolic leaf extract using standard procedures. Different concentrations of the plant extract (20 µg/ml- 100µg/ml) were used to assess its effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical activity, It's reducing power and it total phenolic content. Lorke’s method of acute toxicity was adopted for the acute toxicity study. Results obtained showed the presence of saponins, tannins, flavonoids, cardiac glycosides, steroids, and carbohydrates in the leaf extract. The ethanolic leaf extract of IG significantly (P< 0.05) inhibited the activity of DPPH when compared with the vitamin C standard. IG leaf extract also showed a higher reducing power as compared with the Vitamin C standard. The total phenolic content of IG ethanolic leaf extract was however, significantly (P<0.05) lower than that of gallic acid, the standard used. Futhermore, the LD50 of the ethanolic leaf extract was found to be above 5000mg/kg body weight. Irvingia gabonensis (O’Rorke) baill ethanolic leaf extract is a rich source of important phytochemicals and possesses a high antioxidant activity. Also, the administration of the ethanolic leaf extract in wistar rats is safe up to a dose of 5000mg/kg body weight.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"118 1","pages":"36-41"},"PeriodicalIF":0.0,"publicationDate":"2016-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81456671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Effect of mercury (HG) on some biological parameters of channa punctatus 汞对马尾鱼某些生物学参数的影响

International journal of biological research in pregnancy Pub Date : 2016-03-23 DOI: 10.14419/IJBR.V4I1.5910

Y. Gupta, Pravesh Kumar

引用次数: 1

Phylogenetic information of freshwater copepod (Diaptomus sicilis) with special reference to 18S rRNA 淡水桡足动物(Diaptomus sicilis)的系统发育信息，特别参考18S rRNA

International journal of biological research in pregnancy Pub Date : 2016-03-12 DOI: 10.14419/IJBR.V4I1.5836

Gomathi Jeyam Mookkaiah, R. Ravichandran

{"title":"Phylogenetic information of freshwater copepod (Diaptomus sicilis) with special reference to 18S rRNA","authors":"Gomathi Jeyam Mookkaiah, R. Ravichandran","doi":"10.14419/IJBR.V4I1.5836","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5836","url":null,"abstract":"In the present investigation to isolate freshwater calanoid copepods ( Diaptomus sicilis ) was characterized and identify the organisms by 18S rRNA sequencing. Plankton samples containing D. sicilis were collected during January 2014 (Post-monsoon) from Madippakkam Lake (12°57'41\"N80°11'27\"E) Chennai, Tamil Nadu. Immediately after sampling, specimens for genetic analyses were fixed in 95% ethyl alcohol. The total DNA was extracted from the individual copepod D. sicilis using Qiagen Blood tissue kit. The nuclear small subunit 18S rRNA gene was amplified using the Universal primer LCO —1490 (5’-GGTCAACAAATCATAAAGATATTGG-3’) and HCO-2198 (5’-TAAACTTCAGGGTGACCAAAAAATCA-3’). PCR products were loaded onto a 1% TAE agarose gel. Sequences were carried out an automated sequencer. The nucleotide sequence of 1282 base pair region of 18S rRNA was determined for D. sicilis. The similarity of sequences of D. sicilis was retrieved by BLASTn program and maximum identity and E-value was 76% and 0.00, respectively. The PCR products of D. sicilis individuals showed 80% similarity with the partial nuclear small subunit 18S rRNA gene region of other calanoid copepods. Based on molecular data the freshwater Calanoid copepods showed different algorithms and similar types of topologies useful for designing molecular analyses using phylogeny tree construction.Present molecular studies on the relationship of D. sicilis with other freshwater calanoid copepods indicate that this species is close to D. castor followed by D. keniraensis .","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"00 1","pages":"25-28"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79022523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microbiological and physicochemical properties of commercial seal tampered refrigerated fruit juices 商品密封篡改冷藏果汁的微生物学和理化性质

International journal of biological research in pregnancy Pub Date : 2016-02-26 DOI: 10.14419/IJBR.V4I1.5798

Olusola A Oluwole, O. M. David, C. Falegan, Biodun Awojuyigbe, O. M. Olajide

{"title":"Microbiological and physicochemical properties of commercial seal tampered refrigerated fruit juices","authors":"Olusola A Oluwole, O. M. David, C. Falegan, Biodun Awojuyigbe, O. M. Olajide","doi":"10.14419/IJBR.V4I1.5798","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5798","url":null,"abstract":"Microbial and physicochemical properties of seven branded, seal tampered refrigerated fruit juices were carried out in this study using standard methods. Coliform counts ranged from 2.079 to3.093 log 10 cfu/ml over the storage period with pineapple juice and citrus juice having the highest and least coliform count respectively. Total bacteria count in the juice ranged from 7.009 to 8.243 log 10 cfu/ml. Citrus fruit juice however had the highest staphylococcal count while pineapple juice had the least (2.344 to 3.881log 10 cfu/ml). Also, osmophilic yeast count ranged from 2.017 to 3.903log 10 cfu/ml, having the highest load in orange fruit juice and lowest load in citrus fruit juice. The pH of the juice samples ranged from 2.9 to 4.2 during the period of refrigeration. Conductivity was highest in apple fruit juice and lowest in orange nectar pulp fruit juice. The total dissolved solids ranged from 0.29 to 1.95 over storage and was recorded highest in apple juice and lowest in orange nectar pulp fruit juice. Turbidity ranged from 5.8-200. These results indicate a reduction in the quality of fruit juices after 5 days of opening and thus reveals that both spoilage and pathogenic organisms could proliferate in juices despite refrigeration.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1 1","pages":"21-24"},"PeriodicalIF":0.0,"publicationDate":"2016-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82460138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Dimethylnitrosamine (DMN) exposed rats: Vernonia amygdalina pre-treatment enhances immunity, hepatic and renal function 二甲基亚硝胺(DMN)暴露大鼠:苦杏仁果预处理增强免疫、肝肾功能

International journal of biological research in pregnancy Pub Date : 2016-02-24 DOI: 10.14419/IJBR.V4I1.5811

Usunomena Usunobun, N. Okolie

{"title":"Dimethylnitrosamine (DMN) exposed rats: Vernonia amygdalina pre-treatment enhances immunity, hepatic and renal function","authors":"Usunomena Usunobun, N. Okolie","doi":"10.14419/IJBR.V4I1.5811","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5811","url":null,"abstract":"Background: The occurrence of dietary and environmental chemicals such as dimethylnitrosamine (DMN) in drinks and foods including fish and meat as well as fresh supermarket products is well established. This study evaluated protective effect of ethanolic leaf extract of Vernonia amygdalina (VAE) on liver synthetic molecules, kidney function and hematological parameters in acute dimethylnitrosamine (DMN)-induced hepatic toxicity in wistar male rats. Methods: Experimental rats divided into four groups of six rats each were used. The first group was untreated and served as control. The second group was orally administered VAE (400 mg/kg) only for seven days. The third group was pre-treated with VAE (400mg/kg) for 7 days and administered 20mg/kg DMN 24hrs after VAE pre-treatment. Rats of the fourth group were given 20mg/kg DMN alone same time with that of group 3. All rats were sacrificed 48hrs after DMN administration. Results: In rats administered 20mg/kg DMN, VAE pre-treatment at 400 mg/kg significantly increased total protein, albumin,White blood cell (WBC), Red blood cell (RBC), Hemoglobin (Hb), packed cell volume (PCV) and Platelets while it significantly decreased total bilirubin, urea and creatinine compared to DMN-alone administered rats. Conclusion: This study suggest that VAE pre-treatment exert its ameliorative effect against DMN-induced hematological and biochemical alterations possibly by preventing the decline of antioxidant defense system and could be prescribed as adjunct to dietary therapy.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"14 1","pages":"17-20"},"PeriodicalIF":0.0,"publicationDate":"2016-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88005192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1