International journal of biological research in pregnancy最新文献_第7页

Evaluation of antimycobacterial rhamnolipid production from non-cytotoxic strains of Pseudomonas aeruginosa isolated from rhizospheric soil of medicinal plants 药用植物根际土壤中无细胞毒性铜绿假单胞菌产鼠李糖脂的研究

International journal of biological research in pregnancy Pub Date : 2016-08-06 DOI: 10.14419/IJBR.V4I2.6429

A. Mishra, R. Dubey, Shivraj M. Yabaji, S. Jaiswal

{"title":"Evaluation of antimycobacterial rhamnolipid production from non-cytotoxic strains of Pseudomonas aeruginosa isolated from rhizospheric soil of medicinal plants","authors":"A. Mishra, R. Dubey, Shivraj M. Yabaji, S. Jaiswal","doi":"10.14419/IJBR.V4I2.6429","DOIUrl":"https://doi.org/10.14419/IJBR.V4I2.6429","url":null,"abstract":"Rhamnolipids (RLs) are the bacterial derived biosurfactants and known for a wide range of industrial and therapeutic applications. They exhibit potent anti-bacterial activity against various gram positive, gram negative and acid fast bacteria including Mycobacterium tuberculosis . Since, Pseudomonas is one of the largest known genuses containing a variety of rhamnolipid producing strains. Therefore, in this study, we selectively isolated the Pseudomonas aeruginosa strains from the rhizospheric soil of the Indian plants of medicinal value, e.g. Azadirachta Indica and Ficus spp ., and evaluated them for their natural ability to produce antibacterial rhamnolipids. The bacteria were identified on the basis of 16s rRNA sequencing and biochemical characterization. Among 33 of P. aeruginosa isolates from different soil samples, four isolates showed potent inhibitory activity against methicillin resistant Staphylococcus aureus (MRSA) and fast grower mycobacterial spp. The inhibitory potential of the isolates was found to be correlated with their ability to produce RLs in the medium. The industrial viability of the strains was assessed on the basis of cytotoxicity determining alternative allele, exoS/exoU and cell mediated cytotoxicity against murine macrophages J774.1. The newly isolated strains harbor exoS allele and exhibits lower cell mediated cytotoxicity on macrophage cell line as compared to the clinical strains PA-BAA-427 and PA-27853 used as a control in this study. Evaluation of antimycobacterial rhamnolipid production from non-cytotoxic strains of Pseudomonas aeruginosa isolated from rhizospheric soil of medicinal plants","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"11 2 1","pages":"112-118"},"PeriodicalIF":0.0,"publicationDate":"2016-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82791119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Artificial rubber mineralization by co-cultured bacterial strains 共培养菌株对人造橡胶矿化的影响

International journal of biological research in pregnancy Pub Date : 2016-08-02 DOI: 10.14419/IJBR.V4I2.6414

Manasasri Muralidharan, V. Krishnaswamy

{"title":"Artificial rubber mineralization by co-cultured bacterial strains","authors":"Manasasri Muralidharan, V. Krishnaswamy","doi":"10.14419/IJBR.V4I2.6414","DOIUrl":"https://doi.org/10.14419/IJBR.V4I2.6414","url":null,"abstract":"Objective: This study involves the use Natural Rubber degrading Bacterial co-cultures isolated from rubber plantation soil for the degradation of Artificial Rubber. Methods: Plate assay method and liquid assay method by using Mineral Salt Medium was followed for screening of bacteria for its capacity to mineralize Artificial rubber. Degradation was confirmed by Spectrophotometric and Fourier Transform Infra-Red (FTIR) studies. The co-cultures were able to utilize the artificial rubber which was confirmed by Spectrophotometric and FTIR studies. Results: Bacterial cocultures have the capacity to mineralize artificial rubber. Mineralization was observed for 30 days and it was maximum (6.48 x 10-5) on the 20th Day. This was confirmed using SEM and FTIR.Further, these were applied on bioplastics and plastics to study their capacity to degrade them. It was able to degrade them as well which was confirmed using FTIR. Conclusion: From current investigation, it can be concluded that the bacterial cocultures Bacillus cohnii and Brevundimonas naejangsanensis have the capacity to mineralize artificial rubber, plastics and bioplastics. Hence such isolated cocultures can be used in removal of artificial rubber, plastics and bioplastic wastes from the environment.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"40 1","pages":"105-111"},"PeriodicalIF":0.0,"publicationDate":"2016-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76217353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Composting process: a review 堆肥过程:回顾

International journal of biological research in pregnancy Pub Date : 2016-07-31 DOI: 10.14419/IJBR.V4I2.6354

S. Raza, J. Ahmad

引用次数: 24

Characterization of best naringinase producing fungus strain isolated from palmyrah (Borrasus flabellifer) fruit pulp 棕榈果浆产柚皮苷酶最佳菌株的鉴定

International journal of biological research in pregnancy Pub Date : 2016-07-19 DOI: 10.14419/IJBR.V4I2.6289

Sinthuja Karuppaija, K. Ranganathan, Vasantharuba Seevaratnam

{"title":"Characterization of best naringinase producing fungus strain isolated from palmyrah (Borrasus flabellifer) fruit pulp","authors":"Sinthuja Karuppaija, K. Ranganathan, Vasantharuba Seevaratnam","doi":"10.14419/IJBR.V4I2.6289","DOIUrl":"https://doi.org/10.14419/IJBR.V4I2.6289","url":null,"abstract":"Background: The Palmyrah ( Borrasus flabellifer L.) fruit pulp has the bitter compound flabelliferin (a tetraglycoside) which can be hydrolyzed by naringinase enzyme. The diverse groups of filamentous fungi and bacteria that live in different substrates have the capacity of producing extracellular naringinase enzyme which is of tremendous industrial value. Objective: The objective of the study was to isolate the naringinase producing fungal strains from Palmyrah and to identify the best naringinase producer under liquid and solid state fermentation systems. Methods: Fungal strains isolated from Palmyrah fruit pulp and the soil where pulp is allowed to decay, were grown on naringin agar selective medium at pH 6.0 at room temperature and the production of extracellular naringinase was measured in the liquid fermentation media and solid state fermentation system using paddy husk as support. Results: Five fungal strains isolated from the palmyrah pulp and the pulp decaying in sand designated as PF1,PF2,PF3,PF4 & PF5 had the ability to produce extracellular naringinase enzyme in liquid fermentation media. Fungal strain PF4 that showed highest naringinase enzyme activity (1.769U/ml) was selected among the isolated five fungal strains and identified as Rhizophus stolonifer based on the morphological and biochemical characteristics. When this strain was grown in the solid state fermentation system using paddy husk as media, narininase production was higher (269.84 U/gram of dry substrate) in seven days. Conclusion: Rhizophus stolonifer could be used to produce large scale naringinase enzyme under solid state fermentation system using very cheap, easily available, agricultural waste paddy husk as support without the need of expensive and well equipped laboratories.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"20 1","pages":"97-101"},"PeriodicalIF":0.0,"publicationDate":"2016-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89258605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Grazing intensity contributes to cyanogenic toxicity in savannah grasses in Baringo county 放牧强度对巴林戈县大草原草地的氰化毒性有影响

International journal of biological research in pregnancy Pub Date : 2016-06-28 DOI: 10.14419/IJBR.V4I2.6240

Alfayo Koskei, E. Maranga, M. Obonyo

{"title":"Grazing intensity contributes to cyanogenic toxicity in savannah grasses in Baringo county","authors":"Alfayo Koskei, E. Maranga, M. Obonyo","doi":"10.14419/IJBR.V4I2.6240","DOIUrl":"https://doi.org/10.14419/IJBR.V4I2.6240","url":null,"abstract":"The potential role of anti-herbivory mechanisms used by plants and their synergistic responses to grazing and interactive effects on herbivores are poorly understood. The aim of this study was to quantify the influence of grazing intensity on cyanogenic glycosides in Lake Bogoria, Baringo County Kenya. Field experiments were carried out in ten 50×10m enclosures. Grazing intensity was varied using simulated grazing method where two grazing treatments used; heavy grazing and light grazing. Grasses were categorized into two age classes; young and old. Cyanigenic glycocides (CNglc) were tested using impregnated picrate paper and their concentration determined by hydrolysis and trapping in 1M NaOH. Our findings showed that five of 16 sampled species produce cyanogenic glycosides; Cynodon dactylon, Cynodon plectostachyus, Digitaria scalarum, Sporobolus spicatus and Cyperus laevigatus. There was an inverse relation between Cyanide concentration and age of the plants. Young cuttings yield more Hydrogen Cyanide than older cuttings of the same grasses.Grazing intensity had a significant effect on the concentration of cyanogenic content in some grass species; C.dactylon (P=0.024) and S. laevigatus (P=0.003). The findings imply that grazing regime of managed pastures should consider the age of forage while allowing utilization of pastures preferably grazed on mature pastures with low levels of cyanogenic glycosides.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"7 1","pages":"91-96"},"PeriodicalIF":0.0,"publicationDate":"2016-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90500372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vermiconversion of paper industry waste for recycling the nutrients usig earthworm eudrilus eugeniae kingberg on growth of cluster bean (cymopsis tetragonoloba) 利用eugenae kingberg蚯蚓转化造纸废弃物回收营养物对集束豆生长的影响

International journal of biological research in pregnancy Pub Date : 2016-06-27 DOI: 10.14419/IJBR.V4I2.6280

P. Sivakumar, R. Chithra

{"title":"Vermiconversion of paper industry waste for recycling the nutrients usig earthworm eudrilus eugeniae kingberg on growth of cluster bean (cymopsis tetragonoloba)","authors":"P. Sivakumar, R. Chithra","doi":"10.14419/IJBR.V4I2.6280","DOIUrl":"https://doi.org/10.14419/IJBR.V4I2.6280","url":null,"abstract":"Objective: To preparation of vermicompost from paper industry waste for recycling the nutrients using earthworm Eudrilus eugeniae kingberg. To analyze the physico chemical parameters of vermicompost, vermiwash and vermicompost extract, enumeration of microorganism from vermicompost, growth parameters and biochemical characteristics of Cluster bean (Cymopsis tetragonoloba) were studied. Methods: The paper industry wastes washed for two times with preparing the predecompost and it kept in 30 days. For preparing the vermicompost, the predecomposition was directly mixed with cowdung in the ratio of 1:2 on dry weight basis in same tank. Seventy number of healthy, clitellate Eudrilus eugeniae kingberg were introduced in the same tank. After 45 days the trial tank compost were sieved and collected for paper industry waste vermicompost. Results: The physico chemical parameters of vermicompost vermiwash and vermicompost extract were studied, the growth parameters and biochemical characteristics were higher in treatments 5 and 6 of Cluster bean were using various concentration of vermicompost, vermiwash and vermicompost extract. Conclusion: Present study was concluded that the Eudrilus eugeniae kingberg is more efficient in bioconversion of paper industry waste vermicompost, vermiwash and vermicompost extract were using various concentration of pot culture study of brinjal was higher growth and high yield and vermicompost act as an excellent biofertilizer of crop plants.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"133 1","pages":"88-90"},"PeriodicalIF":0.0,"publicationDate":"2016-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77981925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of best naringinase producing fungus isolated from the citrus fruits 柑橘果实中产柚皮苷酶最佳真菌的鉴定

International journal of biological research in pregnancy Pub Date : 2016-06-22 DOI: 10.14419/IJBR.V4I2.6281

K. Srikantha, K. Ranganathan, Vasantharuba Seevaratnam

{"title":"Characterization of best naringinase producing fungus isolated from the citrus fruits","authors":"K. Srikantha, K. Ranganathan, Vasantharuba Seevaratnam","doi":"10.14419/IJBR.V4I2.6281","DOIUrl":"https://doi.org/10.14419/IJBR.V4I2.6281","url":null,"abstract":"Naringinase enzyme has potential application in food and pharmaceutical industry. Naringin and limonin are principle bitter components in the citrus fruit. The microorganisms that associate with citrus fruit may have the ability to degrade the naringin by extracellular naringinase enzymes that are produced by microorganisms. The objective of the study is to isolate naringinase producing fungus from the citrus fruit to debitter the citrus juice and to characeterize the fungus. Citrus fruits were allowed to spoil under the air and soil and the lesion was used to streak on fresh PDA plates. Out of the eight strains isolated from citrus fruits, five were positive for naringinase enzyme. When all the naringinase producing fungi were subjected to liquid fermentation medium for eight days at room temperature at 200 rpm and the crude enzyme was tested for naringinase enzyme at pH 5 and 50 oC for 10 minutes, one strain showed the best naringenase activity (1.92 µmol/ml/min). This strain was identified as Aspergillus flavus based on the macroscopic, microscopic and biochemical tests. The culture conditions were optimized to increase the naringinase production via solid state fermentation system using paddy husk as the support. Though naringinase activity of Aspergillus flavus has started on the 2nd day, the highest activity (449.58Ug -1 Dry Matter) was obtained on the 8th day. Thereafter the naringinase activity has started to decline. Solid state fermentation using paddy husk as support could be used for large scale naringinase enzyme production.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"76 1","pages":"83-87"},"PeriodicalIF":0.0,"publicationDate":"2016-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79987157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Diversity of medicinal plants in homegardens in Tanjung Julu village, North Sumatra, Indonesia 印度尼西亚北苏门答腊Tanjung Julu村家庭花园里药用植物的多样性

International journal of biological research in pregnancy Pub Date : 2016-05-27 DOI: 10.14419/IJBR.V4I1.6145

M. Silalahi

引用次数: 2

Production process and characterization of extra cellular li- pids from bacterial strain from oil industries waste 石油工业废弃物细菌胞外脂的制备工艺及特性研究

International journal of biological research in pregnancy Pub Date : 2016-05-24 DOI: 10.14419/IJBR.V4I1.6161

V. Balamurugan, V. Balakrishnan, A. Sundaresan, K. Vasanthi, S. Venkatesan

引用次数: 0

Production of ethanol and clarification of apple juice by pectinase enzyme produced from Aspergillus terreus NCFT 4269.10 用土曲霉nft 4269.10产的果胶酶生产乙醇及澄清苹果汁

International journal of biological research in pregnancy Pub Date : 2016-05-22 DOI: 10.14419/IJBR.V4I1.6134

B. Sethi, Amrita Satpathy, S. Tripathy, Sidarth Parida, Sameer K. Singdevsachan, B. Behera

引用次数: 11