{"title":"Poronia radicata Hembrom, A. Parihar & K. Das, A new record from Maharashtra State, India","authors":"P. Rajendra, P. Prakash","doi":"10.14419/IJBR.V4I1.5684","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5684","url":null,"abstract":"Family Xylariaceae is represented from India by 187 species belonging to 12 genera till date. Genus Poronia Willdenow ex SF Gray was reported for the first time from Kolkata in 2013. During the present mycological exploration, the species Poronia radicata Hembrom, A. Parihar & K. Das, a coprophillous member of family Xylariaceae was collected and studied. This is the second report from the Indian sub-continent and first from state of Maharashtra, India. Thus, it makes a new report to the fungi of Maharashtra.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1 1","pages":"14-16"},"PeriodicalIF":0.0,"publicationDate":"2016-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84608247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Marital fidelity and congregation of Indian sarus crane, Grus antigone antigone in and around Alwara lake of district Kaushambi (Uttar Pradesh), India","authors":"S. Prakash, A. Verma","doi":"10.14419/IJBR.V4I1.5692","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5692","url":null,"abstract":"Sarus crane is a monogamous bird and well known as an eternal symbol of unconditional love, devotion and good fortune. Its occurrence represents a healthy wetland ecosystem. These cranes are large, long-legged and long-necked birds belonging to family: Gruidae, order: Gruiformes, class: Aves and phylum: Chordata. Indian Sarus Crane, Grus antigone antigone is the largest of the crane species found in India. These water birds prefer open habitat like marsh areas, abundantly irrigated paddy fields, grass land and river banks as these areas suit them for foraging, roosting and nesting. It has been showing an increasing population trends in this wetland because its habitat is well suited for its population growth. The present study concerns the survey of Indian Sarus Crane in and around Alwara Lake of District Kaushambi (U.P.) during 2014 with special reference to marital fidelity and congregation. The authors reported a congregation of maximum up to 155 cranes during the study period.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"10 1","pages":"10-13"},"PeriodicalIF":0.0,"publicationDate":"2016-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84207798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mineralisation of natural rubber (poly cis 1-4 isoprene) by co-cultured bacterial strains isolated from rubber plantation area","authors":"Manasasri Muralidharan, V. Krishnaswamy","doi":"10.14419/IJBR.V4I1.5552","DOIUrl":"https://doi.org/10.14419/IJBR.V4I1.5552","url":null,"abstract":"Objective: To isolate Natural Rubber degrading Bacterial co-cultures from rubber plantation soil and to characterize and identify the organisms by 16s r RNA sequencing. Methods: Cocultures of the bacteria were isolated from the contaminated site by enrichment culture technique. Plate assay method and liquid assay method by using Mineral Salt Medium was followed for screening of bacteria for its capacity to mineralize Natural rubber. Degradation was confirmed by Spectrophotmetric and Fourier Transform Infra-Red (FTIR) studies.Natural rubber degraded by the cocultures were studied at different concentrations and the physico-chemical analysis were optimized (pH, temperature, carbon and nitrogen sources) Results: Isolated organism was identified as Bacillus cohnii and Brevundimonas naejangsanensis . The co-cultures were able to utilize the Natural rubber which was confirmed by Spectrophotometric and FTIR studies. From the current study it was evaluated that Natural rubber was mineralized up to 50 % where optimum concentration was 10 %. Conclusion: From current investigation, it can be concluded that our isolated bacterial cocultures Bacillus cohnii and Brevundimonas naejangsanensis have the capacity to mineralize Natural rubber and hence such isolated cocultures can be used in removal of from waste Natural rubber products in the environment.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"13 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76907515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Presence and antibiotic susceptibility of Listeria monocytogenes in retail meat and meat products","authors":"Y. Doğruer, N. Telli, A. E. Telli, A. Güner","doi":"10.14419/IJBR.V3I2.5323","DOIUrl":"https://doi.org/10.14419/IJBR.V3I2.5323","url":null,"abstract":"In this study total of 200 samples including red meat, ready to eat meat (RTE) and traditional red meat products were taken from butcher shops and supermarkets and analyzed for the presence of L. monocytogenes . Presence of Listeria spp. was investigated with cultural and PCR methods. Susceptibility of the isolates to 18 antibiotics were determined by disk diffusion method. 19 out of 200 samples (9.5 %) were found to be contaminated with Listeria spp. The isolates were identified as L.monocytogenes, L. innocua , L. seeligeri , L. welshimeri , L. ivanovii ; 22.10 %, 55.79 %, 11.58 %, 6.32 %, 4.21 % respectively. L. monocytogenes were isolated from meat pieces (2/40), minced meat (3/40) and hamburger (1/20). All of the L. monocytogenes isolates were susceptible to three antibiotics (Amoxycillin/Clavulonic acid, Sulphamethoxazole/ Trimethoprim and Vancomycin) and resistance to one antibiotic (Clindamicin). As a result, it was evaluated that minced meat and meat pieces was the highest rate (83.3 %, 5/6) of contamination with L. monocytogenes. Determination of non-pathogenic Listeria spp. is found to be important because of the indicator of L. monocytogenes . Hereby, the results presented in this study indicated the potential risk of raw meat and meat products on infection with L. monocytogenes.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"24 1","pages":"76-81"},"PeriodicalIF":0.0,"publicationDate":"2015-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73902642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. B. Mukiese, Aimé Diamuini Ndofunsu, F. Bulubulu, Alexandre Mbaya Ntumbula, S. Ndiku
{"title":"In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)","authors":"J. B. Mukiese, Aimé Diamuini Ndofunsu, F. Bulubulu, Alexandre Mbaya Ntumbula, S. Ndiku","doi":"10.14419/IJBR.V3I2.5197","DOIUrl":"https://doi.org/10.14419/IJBR.V3I2.5197","url":null,"abstract":"Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava ( Manihot esculenta Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated in vitro in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"54 1","pages":"72-75"},"PeriodicalIF":0.0,"publicationDate":"2015-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73515383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stéphanie Beaudelaine Kengni, F. T. Fohouo, A. Ngakou
{"title":"Impact of the foraging activity of Apis mellifera adansonii Latreille (Hymenoptera: Apidae) and Bradyrhizobium fertilizer on pollination and yield components of Glycine max L. (Fabaceae) in the field","authors":"Stéphanie Beaudelaine Kengni, F. T. Fohouo, A. Ngakou","doi":"10.14419/IJBR.V3I2.5211","DOIUrl":"https://doi.org/10.14419/IJBR.V3I2.5211","url":null,"abstract":"To determine the impact of Apis mellifera adansonii and Bradyrhizobium on pod and seed yields of Glycine max , field trials were carried out during 2012 and 2013 cropping seasons. Hence, 120 to 25658 flowers were labeled each year and divided into five treatments, differentiated according to whether plots were inoculated with Bradyrhizobium or not, or plants were protected from insects activities or not and the last treatment with flowers isolated then opened only to A. m. adansonii . The effects of Bradyrhizobium on nodulation, plant biomass and seed yield, as well as the foraging behavior of A. m. adansonii on flowers, the number of seeds per pod and the normal seeds' rate were evaluated. Results indicate that Bradyrhizobium significantly increased the number of flowers (P<0.001), root nodules (P<0.0001), plant biomass (P<0.0001), pod and seeds yields in inoculated plots. A. m. adansonii foraged on G. max flowers from 09.00 a.m. to 16.00 p.m. and throughout the whole blooming period. This insect intensely harvested only nectar. By comparing the yields of unprotected flowers to those of flowers isolated then opened to A. m. adansonii , 35.85% increase fructification index, and 73.09% increase in the number of seeds per pod due to this bee were recorded. The synergistic activity of insects and Bradyrhizobium increased the number of seeds per pod by 32.16% and the percentage of normal seeds by 32.87%. Our results reveal that inoculation of soybean plant at sowing with Bradyrhizobium and installation of hives close to the field could be recommended for a sustainable pods and seed yield improvement of this crop.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"54 1","pages":"64-71"},"PeriodicalIF":0.0,"publicationDate":"2015-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88731911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A cassava mosaic disease epidemiologic factors analysis in the Bas-Congo (DR Congo) zone of savannah. case study: “Secteur de Boko”","authors":"Bansimba Mukiese J.-Roger","doi":"10.14419/ijbr.v3i1.4069","DOIUrl":"https://doi.org/10.14419/ijbr.v3i1.4069","url":null,"abstract":"This paper aims at evaluating epidemiologic parameters of the African Cassava Viral Mosaic Disease (CMVD) in 25 cassava farmer's fields of «Secteur de Boko» (DR Congo) on the basis of foliar symptoms observation. The results showed that the infection of the local varieties comes mainly from the cuttings whereas that of the improved varieties comes from the flies. The disease incidence varied between 10% and 88.33% with an average of 43.8%. Severity varied between 2.42 and 3.42 with an average of 2.913. The gravity varied between 17.86% and 87.81% while the systemicity varied between 21.42 and 96.66%. The mean number of whiteflies ( Bemisia tabaci ) by plant (2.068) revealed a preference of the vectors for the improved variety Kindombe which, however, presents a low severity and a less marked gravity. Globally, the CMVD was more severe for the local variety Mpeko with a score of 3.00. A high correlation was found between the gravity and the number of neighbour fields (-0,437*), the density of culture (-0,431*) and also the systemicity of the disease (0,779**). In addition; it appeared that the land topography strongly influences the disease severity (-0,542**).","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"36 1","pages":"56-63"},"PeriodicalIF":0.0,"publicationDate":"2015-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77432284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of oxyclozanide (Tremacid®) preparation against fascioliasis on clinical and haematological parameters in cattle of Bangladesh","authors":"Naim Alam, Islam, M. Howlader, N. Lucky","doi":"10.14419/IJBR.V3I1.4486","DOIUrl":"https://doi.org/10.14419/IJBR.V3I1.4486","url":null,"abstract":"The effects of Oxyclozanide (Tremacid®) on some clinical (body weight) and haematological parameters (TEC, Hb, PCV, ESR and TLC) were determined in this study. Among 55 cattle, 10 were selected, which were suffering from fascioliasis and divided randomly into two groups. Each group was consisting of five cattle. One was treated with Tremacid® @ (15 mg/kg body weight). Other was kept as an infected control group. Before trials with Tremacid® initial body weight, EPG of liver fluke and hematological parameters were examined. During the experimental period the faecal samples, clinical and hematological parameters were examined on 7th, 14th, 21st and 28th days for the determination of effects of Tremacid®. A significant reduction of EPG count was found on 7th, 14th, 21st and 28th day of Tremacid® (61.87%, 71.22%, 76.98% and 84.53%) in a treated cattle group. The EPG of an untreated control group was significantly (p<0.01) increased about 3.97%, 7.64%, 11.04% and 64.89% respectively. Total TEC was increased after treatment with Tremacid® and decreased in an untreated control group significantly (p<0.01), Likewise, after treatment with Tremacid® Hb content and PCV were increased, and ESR was decreased. Total leukocyte count (TLC) was decreased and the body weight was increased after Tremacid® treatment in the group A. On the other hand, Hb content PCV and body weight was decreased significantly (p<0.01) in the group B.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"50 1","pages":"46-55"},"PeriodicalIF":0.0,"publicationDate":"2015-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86732190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Aktaruzzaman, M. M. Islam, Z. Mohamed, M. S. Islam, M. Howlader
{"title":"Therapeutic efficacy of ivermectin, fenbendazole and albendazole against naturally occuring gastrointestinal nematodiasis infection in Black Bengal Goat of Bangladesh","authors":"M. Aktaruzzaman, M. M. Islam, Z. Mohamed, M. S. Islam, M. Howlader","doi":"10.14419/IJBR.V3I1.4267","DOIUrl":"https://doi.org/10.14419/IJBR.V3I1.4267","url":null,"abstract":"This study aimed to evaluate the efficacy of ivermectin, fenbendazole and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 50 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 13-15 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200 µgkg-1 body weight, S/C) preparations (Techno Drugs, Limited, Bangladesh) and two solid fenbendazole, albendazole (7.5 mgkg-1 body weight, orally) preparations (Techno Drugs, Limited and Square Pharmaceuticals Ltd, Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7 th , 14 th , 21 st and 28 th day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The therapeutic efficacy was evaluated through determination of parasitic prevalence, body weight gain/loss and hematological findings. Pre and post-treatment EPG (eggs per gram) values were recorded, and efficacies compared. The results showed that the efficacy of ivermectin was 100%, followed by fenbendazole 95.33% and albendazole 90.11%. The observed differences in efficacy between these three brands of anthelmintics were most likely due to variations either in quality or the administered doses. The body weight of the treated animals was slightly increased, which were significant (p 0.05) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p 0.01) in all treated goat and body weight was increased significantly (p<0.01) on day 28. The farm management practices along with results of the present study revealed the efficacy of multiple anthelmintics against gastrointestinal nematodiasis in goat. Additional detailed studies are required to clarify the current status of the efficacy of the anthelmintics widely used in different agro ecologies, animal species and livestock management systems in Bangladesh.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1032 1","pages":"42-45"},"PeriodicalIF":0.0,"publicationDate":"2015-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77201686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md. Arafat Al Mamun, Md Murad Khan, Md. Nahinur Rahmam Akand, S. Khan, M. Hoq
{"title":"Characterization of an alkaline protease with high quality bating potential in leather processing from Bacillus licheniformis MZK05M9 mutant","authors":"Md. Arafat Al Mamun, Md Murad Khan, Md. Nahinur Rahmam Akand, S. Khan, M. Hoq","doi":"10.14419/IJBR.V3I1.4250","DOIUrl":"https://doi.org/10.14419/IJBR.V3I1.4250","url":null,"abstract":"An alkaline protease from Bacillus licheniformis MZK05M9 ( Bl M9), a mutant strain developed in our laboratory, has been partially purified and characterized for its robustness and eco-friendly application potential in processing of hides and skins for leather manufacturing and detergent industries. The enzyme was purified 2.70 fold with specific activity of 1624U/mg in comparison to crude enzyme extract by using ammonium sulfate precipitation, dialysis and Sephadex G-75 column chromatography. The molecular mass of the enzyme was 27.2 kDa as judged by SDS–PAGE. The purified protease had a pH optimum of 8.5 and temperature optimum of 55°C. According to the inhibition profiles obtained with the various protease inhibitors, it was confirmed that the partially purified protease belongs to the serine protease type. The activity of partially purified enzyme was enhanced by calcium, magnesium, barium, potassium and manganese ions and strongly inhibited by mercury ion. In addition, the protease showed remarkable stability in the presence of 1% SDS; 1, 3 and 5% Triton X-100 and H 2 O 2 , which comprise the common bleach-based detergent formulation. The enzyme was found equally efficient to a commercial enzyme Oropon K (one of the commercial enzymes imported into Bangladesh for bating purpose) in bating of animal hide as proved by different comparative qualitative tests such as tensile strength, percent of elongation, stitch tears strength, water vapor permeability, grain crack strength and tongue tear strength tests. In addition, the stability profile (pH, temperature and surfactants) and blood stain removal data also revealed its suitability for application in detergent industry.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1 1","pages":"36-41"},"PeriodicalIF":0.0,"publicationDate":"2015-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81987778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}