Animal genetics最新文献_第4页

MiR-376c-3p Targets STRBP mRNA to Regulate Cashmere Goat Hair Follicle Stem Cell Proliferation and Multipotency by Inhibiting the Wnt/β-Catenin and BMP Signaling Pathways MiR-376c-3p通过抑制Wnt/β-Catenin和BMP信号通路，靶向STRBP mRNA调控绒山羊毛囊干细胞增殖和多能性

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-20 DOI: 10.1002/age.70185

Yi Guo, Niu Wang, Xiaojie Hou, Longkang Sun, Hulin He, Xin Wang

{"title":"MiR-376c-3p Targets STRBP mRNA to Regulate Cashmere Goat Hair Follicle Stem Cell Proliferation and Multipotency by Inhibiting the Wnt/β-Catenin and BMP Signaling Pathways","authors":"Yi Guo, Niu Wang, Xiaojie Hou, Longkang Sun, Hulin He, Xin Wang","doi":"10.1002/age.70185","DOIUrl":"https://doi.org/10.1002/age.70185","url":null,"abstract":"<div>\u0000 \u0000 The quality of cashmere fiber is fundamentally determined by the embryonic morphogenesis of hair follicles, a process highly dependent on the normal function of hair follicle stem cells (HFSCs). MicroRNAs (miRNAs) are key post-transcriptional regulators, but their specific roles in cashmere goat hair follicle development remain unclear. In this study, we identified the differentially expressed miRNA, miR-376c-3p, by analyzing transcriptomic data from embryonic skin (E65, E90, E120) of cashmere goats. Functional assays, including CCK8, crystal violet staining, clonogenic assay, and analysis of proliferation/apoptosis-related proteins (PCNA, CDK4, MKI67, BCL2, CAS3 and CAS9), demonstrated that miR-376c-3p significantly inhibited HFSCs proliferation, induced apoptosis, and blocked the differentiation process, as evidenced by the downregulation of differentiation-associated keratins (KRT4, KRT10, KRT14) and the upregulation of stemness maintenance factors (OCT4, NANOG, SOX9 and KRT15). Mechanistic investigations revealed that miR-376c-3p directly bound to the 3′UTR of STRBP mRNA and inhibited its expression, which was validated by a dual-luciferase reporter assay. Conversely, overexpression of STRBP promoted HFSCs proliferation and differentiation. Further analysis found that STRBP functioned by activating the Wnt/β-catenin and BMP signaling pathways, whereas miR-376c-3p inhibited these same pathways. In summary, this study elucidates a novel regulatory axis: miR-376c-3p influencing the Wnt/β-catenin and BMP signaling cascades in HFSCs by targeting STRBP mRNA, thereby regulating cell fate. This research provides important insights into the molecular mechanisms of miRNA-mediated hair follicle morphogenesis and stem cell biology in cashmere goats.\u0000 </div>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Resequencing of 299 Large White Pigs Identifies RASAL2 as a Candidate Gene for Feed Efficiency Traits 299头大型白猪全基因组重测序鉴定RASAL2为饲料效率性状候选基因

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-20 DOI: 10.1002/age.70187

Lin Chen, Bin Yang, Haoran Shi, Junzhuo Deng, Jing Gao, Yang Liu

{"title":"Whole-Genome Resequencing of 299 Large White Pigs Identifies RASAL2 as a Candidate Gene for Feed Efficiency Traits","authors":"Lin Chen, Bin Yang, Haoran Shi, Junzhuo Deng, Jing Gao, Yang Liu","doi":"10.1002/age.70187","DOIUrl":"https://doi.org/10.1002/age.70187","url":null,"abstract":"<div>\u0000 \u0000 Feed efficiency is a core economic trait in pig breeding, with feed costs accounting for 60%–70% of total production expenses. Deciphering its genetic basis is critical for precise molecular breeding and the development of grain-saving pig breeds. However, most existing studies rely on SNP arrays, which cannot capture insertion–deletion (InDel) variants, and systematic research on modern intensively selected Large White pig populations remains scarce. In this study, we conducted a genome-wide association study (GWAS) for three core traits—feed conversion ratio (FCR), average daily gain (ADG), and average daily feed intake (ADFI)—using whole-genome resequencing data from 299 purebred Large White pigs, with simultaneous detection of genome-wide single nucleotide polymorphisms (SNPs) and InDels. We identified multiple genomic regions significantly associated with feed efficiency traits. An intronic InDel in RASAL2 represented the strongest FCR association signal. As a specific negative regulator of the Ras signaling pathway, RASAL2 positively regulates preadipocyte differentiation and ameliorates high-fat diet-induced insulin resistance, suggesting it may be associated with feed conversion ratio through energy deposition and metabolic homeostasis. We further annotated 11 additional core candidate genes including TAFA5, TMCC3, BTG1, and PLCB1, which are mainly involved in adipogenesis, energy homeostasis, and skeletal muscle development. These findings enrich genetic resources for porcine feed efficiency research and support molecular marker development for genomic selection in Large White pigs.\u0000 </div>","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Approaches to Thoroughbred Horse Identification in Diverse Sample Types Using Multiplexed SNP-PCR Sequencing Panels. 利用多重SNP-PCR测序板在不同样品类型中鉴定纯种马的方法。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70170

Jillian Maniego, Caitlin Harding, Pamela Hincks, Jocelyn Habershon-Butcher, Edward Ryder

{"title":"Approaches to Thoroughbred Horse Identification in Diverse Sample Types Using Multiplexed SNP-PCR Sequencing Panels.","authors":"Jillian Maniego, Caitlin Harding, Pamela Hincks, Jocelyn Habershon-Butcher, Edward Ryder","doi":"10.1002/age.70170","DOIUrl":"10.1002/age.70170","url":null,"abstract":"The ability to determine the identity of equine samples would be highly advantageous in cases of ambiguous sample source, as individual-specific controls for gene editing detection, or forensic examination of suspect biological materials in potential doping cases. To this end, a SNP-PCR sequencing-based panel comprising 93 markers in one reaction was designed and investigated for use in Thoroughbred horses. Marker amplification performance and discriminating power were calculated against a population survey of 264 Thoroughbred whole blood samples from routine doping testing. The sensitivity of the SNP panel was tested on a dilution series and on a variety of matrices, including different types of blood specimen, hair and faeces. No genotyping errors due to allelic dropout were detected down to a starting concentration of 0.25 ng/μL in the dilution series, with both EDTA and lithium heparin anticoagulants performing comparably. Forensic 'crime scene' samples, such as blood on a blood tube label, also performed strongly, although others were more variable due to low DNA concentrations. A mock case of 10 'suspects' and one test blood sample was performed blind by the analyst who correctly identified the source of the sample.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70170"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13418657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Functional SNP in the Promoter Region of Ovine IDO2 Associated With Melatonin Levels via Affecting Transcription Activity by CLOCK. 绵羊IDO2启动子区域的功能SNP通过CLOCK影响转录活性与褪黑激素水平相关。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70173

Xunping Jiang, Shaxuan Chi, Xiaodong Wang, Mengya Chen, Zhuoying Tie, Huiguo Yang, Chaoli Wang, Guiqiong Liu

{"title":"A Functional SNP in the Promoter Region of Ovine IDO2 Associated With Melatonin Levels via Affecting Transcription Activity by CLOCK.","authors":"Xunping Jiang, Shaxuan Chi, Xiaodong Wang, Mengya Chen, Zhuoying Tie, Huiguo Yang, Chaoli Wang, Guiqiong Liu","doi":"10.1002/age.70173","DOIUrl":"10.1002/age.70173","url":null,"abstract":"Sheep seasonal reproduction is regulated by photoperiod via melatonin. Indoleamine 2,3-dioxygenase 2 (IDO2), an enzyme involved in tryptophan metabolism through the kynurenine pathway and belonging to the IDO family, may contribute to melatonin homeostasis, but its role in reproductive seasonality remains unclear. In this study, genome-wide selection analyses combining Z(FST) and XP-EHH between seasonal and non-seasonal sheep identified a selection signature encompassing the IDO2 region and revealed g.-809T>C as a functional candidate variant within this region. Experiments employing electrophoretic mobility shift assay (EMSA) and luciferase assays revealed that this site lies within the CLOCK transcription factor binding domain. The -809C variant reduced promoter activity compared to -809T (p < 0.05). Sheep with the CC genotype had higher plasma melatonin levels than TC/TT counterparts (p < 0.05). These findings suggest that the g.-809C allele decreases IDO2 transcription by reducing CLOCK binding affinity, thereby potentially affecting IDO2-associated tryptophan/melatonin metabolism and increasing melatonin levels, which may contribute to photoperiodic responses in sheep. This IDO2 variation provides new insights into the genetic regulation of melatonin-mediated reproductive seasonality and represents a potential marker for genetic improvement of reproductive seasonal traits in sheep.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70173"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13429496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species Determination Within the Felidae Family Using mtDNA Minibarcoding. 利用mtDNA小条形码技术确定Felidae科的物种。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70181

Kristýna Hebenstreitová, Karolina Mahlerová, Lenka Vaňková, Daniel Vaněk

{"title":"Species Determination Within the Felidae Family Using mtDNA Minibarcoding.","authors":"Kristýna Hebenstreitová, Karolina Mahlerová, Lenka Vaňková, Daniel Vaněk","doi":"10.1002/age.70181","DOIUrl":"10.1002/age.70181","url":null,"abstract":"Wildlife forensic analysis frequently deals with highly degraded DNA samples, including those from tanned hides, processed products of traditional Chinese medicine, and specimens stored in preservation fluids. These samples present significant challenges for traditional DNA barcoding due to the difficulty of amplifying sufficiently long DNA fragments. To overcome this limitation, we designed five primer pairs to amplify short mitochondrial DNA (mtDNA) fragments (~100 base pairs). When aligned collectively, these fragments span approximately 500 bp of the cytochrome C oxidase subunit I (COI) gene, which is one of the most common species-barcoding targets. We subjected the primers to a comprehensive validation process, including in silico analysis and experimental verification using various non-degraded and degraded samples of animal tissues. Here, we demonstrate the efficacy and reliability of our DNA minibarcoding method. By addressing the limitations of traditional DNA barcoding, this method improves the accuracy and success of species identification, thereby supporting wildlife conservation, management, and forensic investigations.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70181"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13462279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148711102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Frameshift Variant in SPAG1 Likely Causes Primary Ciliary Dyskinesia in Cocker Spaniels in Australia. 在澳大利亚，一种新的移码变异SPAG1可能导致可卡犬原发性纤毛运动障碍。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70183

S E Mead, L E Hambrook, R Suryadinata, P Robinson, C M Wade

{"title":"A Novel Frameshift Variant in SPAG1 Likely Causes Primary Ciliary Dyskinesia in Cocker Spaniels in Australia.","authors":"S E Mead, L E Hambrook, R Suryadinata, P Robinson, C M Wade","doi":"10.1002/age.70183","DOIUrl":"10.1002/age.70183","url":null,"abstract":"Primary ciliary dyskinesia (PCD) is a clinical syndrome that in dogs primarily manifests as chronic respiratory disease associated with cilial malfunction. The current study employs whole-genome sequencing and a candidate gene approach to uncover the genetic basis of PCD in three Cocker Spaniel siblings following diagnosis of their respiratory cilia by scanning electron microscopy and high-speed video microscopy. Absence of the disorder in the parents suggested autosomal recessive inheritance. A 29 bp frameshift insertion in the eleventh exon of the candidate gene sperm-associated antigen 1 (SPAG1) [NC_049234.1:g.2213788_2213789insGGCGGCGGCAAGCGGCCGGAGAGGGGCGC] was identified as likely causative for PCD in this family. The 29 bp frameshift variant was unobserved in a public variant call file including 1987 dogs from the Dog10K resource however an in-frame insertion was sometimes observed at the same locus. A Cocker Spaniel with similar symptoms from a different family tested negative for the identified variant suggesting that there are multiple causes for the condition in Cocker Spaniels.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70183"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polygenic and Pleiotropic Adaptation of Yaks to High Altitudes: A Genome-Wide Study of Hematological and Physiological Traits. 高海拔地区牦牛多基因多效性适应：血液生理性状全基因组研究。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70175

Guangming Sun, Ziqi Liu, Hui Jiang, Xin Li, Hui Zhang, Ouzhuluobu, Xuebin Qi, Basangwangdui, Siyu Ge, Yiming Wang, Wangshan Zheng, Yanbin Zhu

{"title":"Polygenic and Pleiotropic Adaptation of Yaks to High Altitudes: A Genome-Wide Study of Hematological and Physiological Traits.","authors":"Guangming Sun, Ziqi Liu, Hui Jiang, Xin Li, Hui Zhang, Ouzhuluobu, Xuebin Qi, Basangwangdui, Siyu Ge, Yiming Wang, Wangshan Zheng, Yanbin Zhu","doi":"10.1002/age.70175","DOIUrl":"10.1002/age.70175","url":null,"abstract":"Hematological adaptation is critical for oxygen transport at high altitudes. While hemoglobin concentration is well-studied, yak (Bos grunniens) hematology versus lowland cattle remains under-explored. We analyzed blood phenotypes from 1244 plateau yaks and 154 lowland cattle. Yaks exhibited elevated hemoglobin, hematocrit, and lymphocyte counts, but reduced platelet counts and monocyte/neutrophil percentages among other parameters, indicating distinct hypoxia adaptation. Using genome-wide association studies (GWAS) and composite of multiple signals (CMS), we identified 6485 selection-associated single-nucleotide variants (SNVs) linked to 43 hematological traits (average 6.85% variance explained). These included 172 yak-selected genes (YSGs), with SLIT3, FSTL5, and PCDH15 showing top pleiotropic effects (SLIT3 regulates immune function and oxygen transport). Thirty-eight YSGs influenced multiple traits, and six (SLIT3, FSTL5, PCDH15, PRIM2, CDH13, HLA-DQB1) overlap with genes under selection in Tibetan highlanders. Crucially, unlike hemoglobin attenuation in humans and other species, yaks show elevated hemoglobin, suggesting an alternative adaptive strategy. Cross-species analysis revealed 76 YSG orthologues affect 13 human blood traits, indicating highland species are likely to have selected the same set of genes for hypoxia adaptation. Our findings demonstrate polygenic, pleiotropic mechanisms underpinning yak adaptation, providing a genomic framework for high-altitude biology and conservation.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70175"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148705010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bta-Novel-miR-357 Inhibits the Proliferation and Promotes Apoptosis of Bovine Myoblasts by Targeting S100A2. Bta-Novel-miR-357通过靶向S100A2抑制牛成肌细胞增殖并促进细胞凋亡

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70172

Gaoqing Xu, Chunqi Hou, He Ding, Hongyu Liu, Jun Wang, Wenfa Lyu

{"title":"Bta-Novel-miR-357 Inhibits the Proliferation and Promotes Apoptosis of Bovine Myoblasts by Targeting S100A2.","authors":"Gaoqing Xu, Chunqi Hou, He Ding, Hongyu Liu, Jun Wang, Wenfa Lyu","doi":"10.1002/age.70172","DOIUrl":"10.1002/age.70172","url":null,"abstract":"The unresolved mechanisms underlying heterosis in beef cattle production traits hinder the optimization of crossbreeding strategies. In this study, we generated Wagyu × Hereford hybrid cattle (F1) and observed significantly enhanced growth rates compared to purebred Hereford controls, prompting further investigation into the epigenetic regulatory basis of heterosis in bovine muscle development. Combined microRNA-seq and RNA-seq profiling of the longissimus dorsi muscle identified 17 differentially expressed miRNAs and 489 differentially expressed genes (DEGs), enabling the construction of a comprehensive miRNA-mRNA regulatory network. Notably, decreased expression of bta-novel-miR-357 (novel-miR-357) and increased expression of S100A2 (S100 Calcium-Binding Protein A2) mRNA were observed in the hybrid cattle. Functional assays in primary bovine myoblasts demonstrated that novel-miR-357 and siS100A2 inhibited cell proliferation and promoted apoptosis. Dual-luciferase reporter assays confirmed that novel-miR-357 directly binds to the 3'UTR of S100A2 mRNA, establishing a targeted regulatory relationship. Furthermore, novel-miR-357 was shown to inhibit proliferation and promote apoptosis of bovine myoblasts through targeting S100A2 mRNA. These findings reveal a novel epigenetic regulatory axis (novel-miR-357/S100A2 mRNA) involved in early muscle development and provide an important molecular mechanism for deeper understanding of the epigenetic regulatory processes underlying heterosis in cattle. Nevertheless, given the polygenic complexity of heterosis, it is important to recognize that this pathway likely represents only one of several contributing factors to this complex trait.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70172"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13430145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Phenotypic and Transcriptomic Dynamics of Hair Follicle Development and Fur Quality in Rex Rabbits. 獭兔毛囊发育和皮毛品质的综合表型和转录组动力学。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70184

Chengpeng Cao, Jingyi Yang, Yanlin Wang, Haoyue Wang, Lei Wang, Bohao Zhao, Xinsheng Wu, Yan Liu, Yang Chen

{"title":"Integrative Phenotypic and Transcriptomic Dynamics of Hair Follicle Development and Fur Quality in Rex Rabbits.","authors":"Chengpeng Cao, Jingyi Yang, Yanlin Wang, Haoyue Wang, Lei Wang, Bohao Zhao, Xinsheng Wu, Yan Liu, Yang Chen","doi":"10.1002/age.70184","DOIUrl":"https://doi.org/10.1002/age.70184","url":null,"abstract":"Rex rabbit fur quality determines economic value, yet the age-dependent dynamics of hair follicle development and their molecular drivers are largely unknown. Here, we performed a time-series integrative analysis combining phenotypic measurements, quantitative histomorphology, and transcriptome sequencing in Yongqing Rex rabbits across 1 to 6 months of age (10 rabbits per month). Fur thickness exhibited a pattern of increase, stabilization, and subsequent increase, with no significant differences between 2M and 4M (p > 0.05). Coat density peaked at 6M, with an average pelt area of 1358.20 ± 52.43 cm2 at 5M and 6M. Fiber length increased and then plateaued, whereas hair diameter showed an increase-decrease-stabilization pattern. Histological analysis revealed a significant age-related decrease in the number of follicle clusters, particularly from 1M to 3M (p < 0.05). Primary hair follicle density was the highest at 1M (p < 0.01) and fluctuated subsequently, whereas secondary hair follicle density increased continuously (p < 0.01). Melanin granules increased significantly from 1M to 4M and peaked at the 4M (p < 0.05). Further, transcriptomic identified 1168, 4204, and 3431 differentially expressed genes (DEGs) for 3M versus 1M, 5M versus 1M, and 5M versus 3M, respectively, with 187 common DEGs including FOXN1, FGF10, and DCN. GO and KEGG analysis indicated significant enrichment in processes such as keratinization, keratinocyte differentiation, ECM-receptor interaction, and the PI3K-Akt signaling pathway. Collectively, our findings reveal the dynamic patterns of hair follicle development in Rex rabbits across phenotypic, histological, and molecular levels, providing a theoretical basis for the genetic regulation of fur quality.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70184"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13473066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Transcriptome and Metabolome Analysis of Meat Quality Differences in Longissimus Dorsi Muscle Between New Zealand White Rabbits and Rex Rabbits. 新西兰大白兔和獭兔背最长肌肉质差异的转录组和代谢组学分析。

IF 2.2 3区生物学

Animal genetics Pub Date : 2026-08-01 DOI: 10.1002/age.70177

Bohao Zhao, Miaocheng Wang, Pinyi Zhao, Ting Chen, Jiawei Cai, Aoyun Fan, Yang Chen, Xinsheng Wu

{"title":"Integrated Transcriptome and Metabolome Analysis of Meat Quality Differences in Longissimus Dorsi Muscle Between New Zealand White Rabbits and Rex Rabbits.","authors":"Bohao Zhao, Miaocheng Wang, Pinyi Zhao, Ting Chen, Jiawei Cai, Aoyun Fan, Yang Chen, Xinsheng Wu","doi":"10.1002/age.70177","DOIUrl":"10.1002/age.70177","url":null,"abstract":"Rabbit meat is widely recognized for its favorable nutritional properties, while the molecular mechanisms underlying differences in meat quality between New Zealand White rabbits and Rex rabbits are essential for rabbit production. In this study, a total of 120 healthy 13-week-old rabbits (60 per group) were used to evaluate meat quality traits. The longissimus dorsi muscle (LDM) was then collected for transcriptomic and metabolomic analyses. The results showed that New Zealand White rabbits exhibited significantly higher live weight before slaughter, eviscerated weight, semi-eviscerated weight, and pH24 (p < 0.01), whereas Rex rabbits displayed higher cooking loss (p < 0.01) and intramuscular fat content (p < 0.05). Metabolomic profiling identified 218 differential metabolites (DMs), which were mainly enriched in amino acid biosynthesis and the pentose phosphate pathway, including key metabolites such as DL-arginine, gallic acid, and lipid-related compounds. Transcriptomic analysis identified 227 differentially expressed genes (DEGs) enriched in pathways associated with muscle development and meat quality, including oxidative phosphorylation, FoxO, and MAPK signaling pathways. Key DEGs, such as MYH13, HOXA13, and PDK4, were associated with muscle fiber formation and fat deposition. Integrated analysis revealed that 34 DEGs and 24 DMs were co-enriched in 26 pathways, with strong correlations observed between oxidative phosphorylation-related genes and energy metabolites, as well as between collagen-associated genes and amino acids. These findings establish a gene-metabolite regulatory network underlying breed-specific differences in meat quality and identify potential molecular markers to improve rabbit meat quality and to better understand muscle metabolism across different rabbit breeds.","PeriodicalId":7905,"journal":{"name":"Animal genetics","volume":"57 4","pages":"e70177"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13433003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0