Andrology最新文献

{"title":"Differential Sperm DNA Methylation Profiles in Healthy Men With High Versus Low Adherence to the Mediterranean Diet in the Led-Fertyl Study.","authors":"Rashmi Agarwal, Nancy Babio, Estefanía Davila-Cordova, María Fernández de la Puente, Cristina Valle-Hita, Carles Arribas, Manel Esteller, Aleix Noguera-Castells, Raquel Pluvinet, Jordi Salas-Salvadó, Albert Salas-Huetos","doi":"10.1111/andr.70398","DOIUrl":"https://doi.org/10.1111/andr.70398","url":null,"abstract":"<p><strong>Background: </strong>Diet is known to play an essential role not only in sperm quality but also in offspring health, likely mediated through the sperm epigenome. Moreover, one of the main components of the Mediterranean diet (MedDiet) (i.e., nuts) have shown to impact sperm DNA methylation patterns.</p><p><strong>Objectives: </strong>This study aims to investigate the association between adherence to the MedDiet and sperm DNA methylation patterns in healthy men.</p><p><strong>Material/methods: </strong>This cross-sectional study was conducted on 60 healthy males of reproductive age (18-40 years) randomly selected, based on the MedDiet adherence level (high vs. low adherence), from 200 participants enrolled for the Lifestyle and Environmental Determinants of Seminogram and Other Male Fertility-Related Parameters (Led-Fertyl) study. Detailed lifestyle and dietary information and semen samples were collected. Sperm DNA was extracted and subjected to bisulfite conversion and then hybridized to Infinium MethylationEPIC v2.0 array according to established protocols. Sperm DNA methylation was analyzed using R statistical software, with CpG sites considered differentially methylated when showing an absolute mean methylation difference ≥ 0.10 between high and low adherence groups and an adjusted p-value < 0.05 RESULTS: There were no significant differences in the general and anthropometric characteristics between participants with high adherence and low adherence to MedDiet. Supervised differential methylation analyses found that there were 42 significantly differentially methylated CpGs, with 17 hypermethylated and 25 hypomethylated CpG sites in the participants with high adherence (n = 30) to MedDiet compared to the participants with low adherence (n = 30), after adjustment for age, smoking status, and BMI. The genes involved within differentially methylated sites underwent a gene ontology (GO) analysis, and we observed that 12 GO terms were significantly enriched (adjusted p-value < 0.05), mainly related to cyclic nucleotide signaling, ion channel activity, membrane trafficking/endocytosis, cytoskeletal organization, and transcriptional regulation.</p><p><strong>Discussion: </strong>These novel and preliminary findings point to a possible relationship between diet quality and the sperm epigenome. Further studies should seek to confirm these observations in larger, more diverse groups including older men and individuals.</p><p><strong>Conclusion: </strong>Overall, our findings suggest that adherence to the MedDiet, a dietary pattern rich in natural methyl donors and cofactors, may modulate sperm DNA methylation at specific genomic loci.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to Letter to the Editor Re: Allan CJ, Sljivich M, Kahan D, Torres C, Palese M, Djordjevic M, Purohit RS. Robot-Assisted Peritoneal Flap Vaginoplasty Versus Penile Inversion Vaginoplasty for Gender Affirming Surgery.","authors":"Chantal J Allan, Rajveer S Purohit","doi":"10.1111/andr.70405","DOIUrl":"https://doi.org/10.1111/andr.70405","url":null,"abstract":"","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Androgens Modulate Tumor-Immune Interactions in Testicular Germ Cell Tumors.","authors":"Ida Hamann, Lucas Klaus, Patrick Fruth, Alexander Fichtner, Tobias J Legler, Holger M Reichardt, Fabian A Gayer","doi":"10.1111/andr.70402","DOIUrl":"https://doi.org/10.1111/andr.70402","url":null,"abstract":"<p><strong>Background: </strong>The majority of testicular germ cell tumors (TGCT) are derived from the germ cell neoplasia in situ and arise after puberty in the local presence of high levels of testosterone. The seminoma subtype features a rich inflammatory infiltrate which impacts tumor progression but the role of testosterone in shaping tumor-immune interactions is yet unknown.</p><p><strong>Objective: </strong>We characterized androgen receptor and associated gene expression in the TGCT microenvironment and investigated the impact of dihydrotestosterone (DHT) on seminomatous tumor cells as well as stimulated T cells and monocytes. As an in vitro model of the seminoma microenvironment, cocultures of TCam-2 cells with immune cell subtypes were analyzed in the presence of DHT.</p><p><strong>Material and methods: </strong>Gene expression was analyzed in tumorous and nontumorous tissue specimens from TGCT patients and TGCT cell lines. Bulk and single-cell RNA-sequencing data were used for validation. T cells and monocytes were isolated from the blood of healthy individuals and either activated in vitro or cocultured with TCam-2 cells. Proliferation and metabolic assays, quantitative RT-PCR, flow cytometry, and ELISA were employed to assess the impact of DHT on tumor and immune cells.</p><p><strong>Results: </strong>Androgen receptor and related gene expression was demonstrated in tumorous tissue from patients, TGCT cell lines, and activated primary immune cells, which was supported by the analysis of published RNA-seq data sets. DHT exerted only a minor effect on TCam-2 cells, whereas hormone treatment markedly mitigated T cell and monocyte activation in a dose-dependent manner. In addition, DHT modulated immune cell activity and selected features of TCam-2 cells in T cell and monocyte cocultures serving as an in vitro model of the seminoma microenvironment.</p><p><strong>Discussion and conclusion: </strong>Our results suggest that androgens present in tumorous testis of TGCT patients could impact tumor-immune interactions and thereby clinical outcome.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-Sectional Study of the PREValence of Erectile DysfunctIon in the Arteriopathic Population (PREVEDI).","authors":"Luca Farchioni, Andrea Ferri, Nicola Leone, Mattia Migliari, Luigi Alberto Maria Bartolotti, Daniele Santi, Giorgia Spaggiari, Roberto Silingardi, Stefano Gennai","doi":"10.1111/andr.70406","DOIUrl":"https://doi.org/10.1111/andr.70406","url":null,"abstract":"<p><strong>Background: </strong>Although the association between erectile dysfunction (ED) and cardiovascular disease is well established, its prevalence in patients with atherosclerosis remains poorly defined.</p><p><strong>Objectives: </strong>To evaluate ED prevalence, severity, and associated risk factors (RFs) in patients with atherosclerotic disease.</p><p><strong>Materials and methods: </strong>This single-centre, cross-sectional, non-randomised observational study included male patients referred to a vascular surgery service with documented atherosclerotic disease. Erectile function was assessed using the International Index of Erectile Function-15 questionnaire, and RFs were analysed using univariable and multivariable regression models.</p><p><strong>Results: </strong>A total of 162 patients were enrolled (mean age, 70.1 ± 8.6 years); ED was present in 93.8%, with severe ED observed in 62.3%. Multivariable analysis confirmed age as a significant RF for ED, particularly > 75 years, while age, diabetes mellitus, and subjective ED duration were significantly associated with greater severity.</p><p><strong>Discussion: </strong>The high prevalence of ED supports its role as a manifestation of systemic vascular disease linked to endothelial dysfunction and diffuse atherosclerosis.</p><p><strong>Conclusion: </strong>ED is highly prevalent among patients with clinically overt atherosclerotic disease requiring vascular surgery referral, and may represent a marker of systemic vascular pathology. Routine assessment of sexual function may improve cardiovascular risk stratification and preventive management.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Cell Clusters to Complexity: Testicular Organoids as Emerging Platforms in Male Reproductive Research and Biotechnological Innovation.","authors":"Merve Gorgulu, Leyla Sati","doi":"10.1111/andr.70397","DOIUrl":"https://doi.org/10.1111/andr.70397","url":null,"abstract":"<p><strong>Background: </strong>Conventional 2D cultures and animal models incompletely recapitulate the human testicular niche, limiting translation for male infertility, toxicology, and endocrine research. Limited and highly variable access to human testicular tissue restricts translational research. Testicular organoids (TOs), self-organizing assemblies of germ and somatic cells within biomimetic scaffolds, microfluidic chips, or bioprinted constructs, can reproduce seminiferous cord/tubule-like organization, elements of the blood-testis barrier and steroidogenic function, and increasingly support germ-cell progression. By integrating defined media, matrix cues, and controlled microenvironments, TO platforms enable mechanism-driven studies and human-relevant new approach methodologies for toxicology, disease modeling, and fertility-preservation research. This, in turn, supports endocrine and reprotoxicology testing, disease modeling, and prepubertal fertility-preservation strategies. Broad and responsible adoption will require clinically concordant read-outs, stable long-term cultures, reproducible and complete human spermatogenesis, and a robust ethical framework. This review aims to comprehensively evaluate TO systems, spanning cell sources, biomaterials, and microenvironmental control to functional read-outs of morphogenesis, steroidogenesis, and germ-cell progression. By synthesizing recent advances, we clarify mechanistic determinants of organoid performance and outline their translational implications for male infertility research, toxicology, and fertility-preservation strategies.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testosterone Undecanoate Significantly Reduces Low Density Lipoprotein Cholesterol and Triglycerides in Men With Adult-Onset Testosterone Deficiency.","authors":"Anirudh Bhat, Arjun Ajith Kumar, Michael Zitzmann, Farid Saad, Carola S König, Karim Sultan Haider, Ahmad Haider, Geoffrey Hackett, Pieter Desnerck, John Marriott, Amar Mann, Richard C Strange, Sudarshan Ramachandran","doi":"10.1111/andr.70393","DOIUrl":"https://doi.org/10.1111/andr.70393","url":null,"abstract":"<p><strong>Background: </strong>Adult-onset testosterone deficiency, a condition characterised by low serum testosterone and hypogonadal symptoms is associated with a higher incidence of cardiovascular disease (CVD) and mortality.</p><p><strong>Objective: </strong>To evaluate associations between change (Δ) in total cholesterol (TC), triglycerides (TG) and low-density lipoprotein (LDL)-cholesterol and testosterone undecanoate (TU) treatment.</p><p><strong>Materials & methods: </strong>We analysed a registry of men with adult-onset TD (409 TU-treated and 415 TU-untreated men, median follow-up 153 months), restricting the analysis to men whose statin treatment was not changed during follow-up, thus avoiding statin related effects. The study cohort comprised the following groups: TU-untreated/not on statins (n = 95), TU-untreated/on statins (n = 245), TU-treated/not on statins (n = 194), and TU-treated/on statins (n = 207). Intra-group non-parametric statistics were performed as baseline characteristics varied between the study groups. We used multivariate and logistic regression models to determine predictors of ∆ lipids (LDL-cholesterol and TG) and mortality.</p><p><strong>Results: </strong>Whilst there was a significant decrease in LDL-cholesterol and TG (mostly in the initial 24 months) in the TU-treated men, the reverse was evident in the untreated men, irrespective of concurrent statin therapy. Baseline LDL-cholesterol and TG inversely predicted ∆ values in the TU-treated men, regardless of statin therapy. LDL-cholesterol at final assessment appeared independently associated with mortality.</p><p><strong>Conclusions: </strong>In this long-term, real-world registry, TC, TG, and LDL-cholesterol decreased in the TU-treated men with baseline values predicting the change, whilst a gradual increase was observed in the TU-untreated men. Using published data, the relative risk reduction in CVD due to LDL-cholesterol reduction is estimated to be around 40%.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the \"Sperm Class Analyzer Computer Aided Sperm Analysis\" System in the Automation of IVF Laboratories.","authors":"Gaetano Santilli, Matteo Verdiglione, Aikaterini Selntigia, Sara Giaracuni, Francesco Gebbia, Mauro Cozzolino, Lucia Maresca","doi":"10.1111/andr.70399","DOIUrl":"https://doi.org/10.1111/andr.70399","url":null,"abstract":"","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal Therapy Before Micro-TESE in Men With Nonobstructive Azoospermia: A Negative Answer to an Overly Broad Question.","authors":"Sandro C Esteves, Arnold P P Achermann, Ricardo Miyaoka, Cassio L Z Riccetto, Adriano Fregonesi","doi":"10.1111/andr.70404","DOIUrl":"https://doi.org/10.1111/andr.70404","url":null,"abstract":"","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to Editor: \"Robot-Assisted Peritoneal Flap Vaginoplasty Versus Penile Inversion Vaginoplasty for Gender Affirming Surgery\".","authors":"Aayat Mahboob, Mohammad Saed Odeh Alhamaydeh","doi":"10.1111/andr.70407","DOIUrl":"https://doi.org/10.1111/andr.70407","url":null,"abstract":"","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Suture Type on Postoperative Outcomes in Minimally Invasive Vasectomy.","authors":"Casey Norton, Daniel R Turin, Bowen Yao, Logan Hubbard, G Charles Ostermeier, Joshua Bodie","doi":"10.1111/andr.70396","DOIUrl":"https://doi.org/10.1111/andr.70396","url":null,"abstract":"<p><strong>Objective: </strong>This study compares vasectomy outcomes between Chromic Gut and Monocryl sutures used for fascial interposition in a large cohort of patients undergoing minimally invasive vasectomy. Current guidelines do not recommend a specific suture material for fascial interposition; therefore, this study aims to evaluate two commonly used sutures to help inform surgical practice.</p><p><strong>Methods: </strong>This retrospective study evaluated 1389 patients who underwent vasectomy by a single surgeon between March 2011 and October 2022. Patients were stratified based on suture type used for fascial interposition: Chromic Gut or Monocryl. The primary outcome was vasectomy failure, defined as the presence of live sperm or >100,000 non-motile sperm/mL on post-vasectomy semen analysis (PVSA). Secondary outcomes included postoperative pain, symptomatic sperm granuloma formation, repeat procedures, and post-vasectomy pain syndrome.</p><p><strong>Results: </strong>Of the 1389 included patients, Chromic Gut was used in 1145 and Monocryl in 244. There were six confirmed failures (0.61%), with no significant difference in failure rate between suture types (p = 1.0). A higher rate of postoperative pain-related follow-up was observed in the Chromic Gut group compared with the Monocryl group (p = 0.045). Additionally, the rate of sperm granuloma and follow-up procedures were higher in the Chromic group, but without statistical significance.</p><p><strong>Conclusion: </strong>This large single surgeon series found no statistically significant difference in vasectomy success between Chromic Gut and Monocryl sutures for fascial interposition. However, an increase in postoperative pain-related follow-up was associated with the use of Chromic Gut. Both materials were associated with low failure rates, consistent with published data.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149029602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}