Andrology最新文献

{"title":"Lower eGFR Associated With Decreased Total Testosterone in Males: Integrated Evidence From Cohorts to Real-World Data.","authors":"Yiding Chen, Kun Wang, Feixiang Yang, Wenbo Hao, Andong Cheng, Qiangsheng Wang, Yu Guan, Jinbiao Zhong, Jialin Meng, Guiyi Liao, Xiansheng Zhang","doi":"10.1111/andr.70209","DOIUrl":"10.1111/andr.70209","url":null,"abstract":"<p><strong>Background: </strong>Chronic kidney disease (CKD) frequently coexists with hypothalamic-pituitary-gonadal dysfunction, yet the exposure-response shape linking kidney function, testosterone, and outcomes remains unclear.</p><p><strong>Methods: </strong>Based on data from five cycles of the National Health and Nutrition Examination Survey (NHANES) and the UK Biobank (UKBB), we used multivariate logistic regression models and linear regression models to explore the associations between total testosterone levels and CKD as well as estimated glomerular filtration rate (eGFR). Subsequently, we plotted restricted cubic splines (RCS) of testosterone levels against the risk of CKD and the eGFR. Finally, we further verified the association between total testosterone levels and eGFR based on the kidney transplant cohort.</p><p><strong>Results: </strong>Across both populations, lower eGFR was revealed to be associated with lower total testosterone in an approximately linear fashion. RCS identified an inflection around the \"borderline low\" range of about 300-400 ng/dL; below that, CKD risk rose steeply, indicating a nonlinear, threshold-like risk pattern. The logistic regression analysis based on the public databases also indicated that CKD would reduce testosterone levels (NHANES: β = -17.98, 95% CI: -29.54, -6.420, p-value = 3.4 × 10^-3; UKBB: β = -12.25, 95% CI: -14.84, -9.661, p-value = 1.9 × 10^-20), but it had no effect on estradiol. In the validation cohort of kidney transplant recipients, total testosterone increased significantly after transplantation, whereas estradiol showed no parallel rise.</p><p><strong>Conclusion: </strong>Our findings demonstrate a clear linear-threshold relationship between declining eGFR and reduced testosterone levels, and show that testosterone significantly increases after renal function recovery in kidney transplant recipients.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2297-2308"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic Intracytoplasmic Sperm Injection (MICSI): A Novel Platform for Sperm Isolation, Selection, and Injection Into Oocytes.","authors":"Mehran Dabiri, Dale Mark Goss, Steven Alexander Vasilescu, Majid Ebrahimi Warkiani","doi":"10.1111/andr.70198","DOIUrl":"10.1111/andr.70198","url":null,"abstract":"<p><strong>Background: </strong>Infertility is a pressing global health concern, affecting one in six couples worldwide. The failure rate for assisted reproductive technologies (ART) cycles remains at approximately 78%, with limited improvements often attributed to a lack of technological innovation. Sperm processing, a crucial component of ART success and offspring health, has seen minimal technical advancement, and current \"gold-standard\" methods such as density gradient centrifugation (DGC) carry risks of iatrogenic injury and DNA damage.</p><p><strong>Objectives: </strong>Microfluidic sperm selection technologies have shown promise in leveraging sperm's unique motility behaviors by mimicking the female reproductive tract's microarchitecture.</p><p><strong>Methods: </strong>In this study, we introduce a microfluidic ICSI (MICSI) platform by integrating microfluidic technology into the form factor of commercially available ICSI dishes, to integrate semen processing, motility and hyaluronic acid (HA) binding-based sperm selection, and oocyte insemination capacity on one single-use consumable. Sperm DNA fragmentation index (DFI), motility, binding score, and sperm morphology were measured to evaluate the performance of the MICSI device compared to DGC and DGC plus HA binding.</p><p><strong>Results: </strong>Practically, the MICSI device was able to select a population of spermatozoa from raw semen with higher progressive motility (p < 0.0001), better morphology (p < 0.0001), and lower DFI (p < 0.0001). When compared to the conventional DGC, the MICSI device produced spermatozoa with significantly higher progressive motility (p < 0.0001) and lower DFI (p < 0.0001).</p><p><strong>Discussion and conclusion: </strong>This data show that the MICSI device may offer a clinically applicable alternative for both selecting high-quality sperm for injection into oocytes and providing a platform to do so.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2252-2261"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146257158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peroxiredoxin 4 Involved in Spermatogenesis by Affecting Oxidative Stress and Ferroptosis.","authors":"Shuning Yuan, Nini Wei, Weiwei Ma, Mengting Hu, Bei Zhang, Li Gao, Chao Gao, Xiaoyu Yang, Yan Meng, Jiayin Liu, Yugui Cui","doi":"10.1111/andr.70221","DOIUrl":"10.1111/andr.70221","url":null,"abstract":"<p><strong>Background: </strong>The main functions of testes are sperm production and androgen secretion in testicular befitting microenvironment. Excessive level of the reactive oxygen species (ROS) from metabolism and cellular events can lead to oxidative stress (OS) and ferroptosis, which injure the functions of mitochondria and endoplasmic reticulum (ER) of testicular cells, leading to the impaired spermatogenesis and spermiogenesis, as well as insufficient androgen. Our previous studies have shown that peroxiredoxin 4 (Prdx4) is a vital ER-located protector against endoplasmic reticulum stress (ERS) in ovarian granulosa cells. In this study, we explored whether Prdx4 played a beneficial role in testes, and the potential application value in male reproduction.</p><p><strong>Methods: </strong>Prdx4 knockout mice and Prdx4^-/y Leydig cells were established using CRISPR/Cas9 system, the natural adult (9 weeks) mice, aging (9 months) mice, and wild-type MLTC-1 cells were used as control. To further investigate the protective effect of Prdx4 in the adult mice, the testicular 43°C water-bath was used to induce an OS model.</p><p><strong>Results: </strong>The male Prdx4^-/y mice aged over 9 months showed the age-related pathology of seminiferous tubule, the increased OS and ERS, and the decreased fertility. The adult Prdx4^-/y mice under heat stress manifested increased levels of OS, including almost 1.5-fold rise in 4-hydroxynonenal (4-HNE) and 8-hydroxy-2'- deoxyguanosine (8-OHdG), higher ferroptosis, and poorer fertility. Prdx4^-/y Leydig cells showed a higher level of ferroptosis after hydrogen peroxide treatment. ACSL4 increased by 18.6% while COX2 increased by 13.5%, and the expressions of GPX4 and SLC7A11 decreased by 49.2% and 24.3%, respectively. Increased levels of ferroptosis in MLTC-1 leads to mitochondria-derived OS and apoptosis, which can be partially restored by deferoxamine and overexpressing SLC7A11.</p><p><strong>Conclusion: </strong>We concluded that Prdx4 as a protective factor plays a critical role in preserving spermatogenesis by alleviating testicular OS and ferroptosis, and that Prdx4 may be known as a potential target in mitigating the adverse effects of natural aging and OS injury.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2329-2346"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147525455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of Gonadal and Extragonadal Germ Cell Tumours in Patients With Klinefelter Syndrome.","authors":"Aksh Tailor, Oishee Bandyopadhyay, Nahian Ahmed, Kapishan Shanmugathasan, Alice Cotton, ChingHao Chen, Tet Yap","doi":"10.1111/andr.70208","DOIUrl":"10.1111/andr.70208","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Klinefelter's syndrome (KS; 47, XXY) is associated with an altered risk profile for malignancies compared with non-KS males. In particular, several reports have noted a striking association between KS and extragonadal germ cell tumours (EGCTs), especially in the mediastinum, whereas the risk of testicular germ cell tumours (TGCTs) in KS remains unclear. KS patients also have a higher prevalence of cryptorchidism (undescended testes)-a known risk factor for TGCT in the general population-yet it is uncertain if cryptorchidism confers the same cancer risk in the KS population. This study aims to compare the incidence of TGCTs and EGCTs in KS against the general population, and to evaluate the impact of cryptorchidism on TGCT risk in KS.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;We conducted a retrospective cohort study of 233 KS patients (age range 2-83, mean 35.1 years) managed at a dedicated KS clinic between 2019 and 2025. Clinical histories, ultrasound findings and histopathology reports were reviewed to identify cases of TGCT, EGCT and cryptorchidism. Incidence rates per 100,000 patient-years were calculated based on 8311 total person-years of follow-up (from birth until age of diagnosis or until end of study period-30th June 2025) and compared with population data from cancer registries. A one-sided Poisson test was used to assess differences in observed tumour incidence versus the general population, with 95% confidence intervals (CIs) derived by exact Poisson methods.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In 233 KS patients, we identified two cases of TGCTs (both testicular tumours in adult men) and one case of mediastinal EGCT. The incidence of TGCT in the KS cohort was 24.1 per 100,000 patient-years (95% CI, 3.0-88.6), which was higher than that in the general male population (∼4.8 per 100,000) but did not reach statistical significance (p = 0.060). In contrast, the incidence of mediastinal EGCT in KS was 12.0 per 100,000 patient-years, exceeding the general population rate (∼0.04 per 100,000). The age-standardised incidence ratios for TGCT was 2.24 (95% CI, 0.27-8.10) compared with the UK population. For EGCT, the crude age-standardised incidence ratio was 83.0 (95% CI, 2.1-460.5). Cryptorchidism was documented in 10 of the 233 KS patients (4.3%). Notably, none of the KS patients with a history of cryptorchidism developed TGCT, and neither of the two KS-TGCT patients had cryptorchidism or other typical risk factors for testicular cancer other than markedly atrophic testes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Discussion: &lt;/strong&gt;KS confers a markedly increased risk of mediastinal EGCT but not a clear increase in TGCT. The pathogenesis of germ cell tumours in KS is multifactorial: aberrant primordial germ cell migration/survival and an altered hormonal milieu are central hypotheses for the predilection towards midline extragonadal tumours, whereas intrinsic testicular degeneration in KS might protect against TGCT despite risk factors like crypto","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2274-2279"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Cryopreserved Sperm Quality and Fertility of Buffalo Through Protein L-Isoaspartyl Methyltransferase Supplementation.","authors":"D S Kumar Wodeyar, S K Bhure, Karikalan M, S K Ghosh, Vikash Chandra, Taru Sharma G","doi":"10.1111/andr.70192","DOIUrl":"10.1111/andr.70192","url":null,"abstract":"<p><strong>Background: </strong>Spermatozoa undergo protein modifications due to oxidative stress as a result of freezing-thawing. With increasing information on oxidatively modified proteins of frozen-thawed spermatozoa, it is essential to investigate the effect of supplementing the semen extender with the repair enzyme protein L-isoaspartyl methyltransferase (PIMT) on the quality of frozen-thawed spermatozoa.</p><p><strong>Objectives: </strong>To investigate the expression of PIMT in male reproductive organs, including semen, and to evaluate the effect of recombinant PIMT (rPIMT) supplementation on the quality of frozen-thawed spermatozoa.</p><p><strong>Materials and methods: </strong>Expression of PIMT was studied using immunofluorescence assays, PCR, and Western blotting. Functionalities of spermatozoa were studied by HOST, viability, progressive motility, acrosome intactness, and zona-binding assays.</p><p><strong>Results: </strong>The PIMT was detected all along the male reproductive tract, including spermatozoa, but could not be detected in the seminal plasma. The rPIMT was added to the extender at 0.5 (Group II), 1.0 (Group III), and 2.0 µg/80 million spermatozoa (Group IV), with the control (Group I) receiving no rPIMT. A significant effect was observed in Group IV, with an 8.9% increase in progressive motility, a 13.0% increase in viability, an 11.9% increase in HOST response, and an 11.8% improvement in acrosome integrity compared with the control. Further, motility and kinematic parameters (VCL, VAP, DSL, VSL, DAP, ALH, and BCF) of frozen-thawed spermatozoa were assessed using CASA. Most motion parameters were higher in group IV compared with the I, II, and III groups. Additionally, the in vitro fertilizability of Group IV spermatozoa was approximately 2.32 times higher compared with the control group.</p><p><strong>Discussion and conclusion: </strong>The better functional attributes and a fold increase in fertilizability of frozen-thawed spermatozoa positively correlate with the quality of spermatozoa. It may be presumed that the rPIMT improved the cryopreserved semen quality through the repair of oxidatively damaged seminal plasma proteins.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2231-2243"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146225172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyglycolic Acid Polymer Versus Porcine Small Intestine Submucosa Graft After Plaque Incision in Peyronie's Disease: A Comparative Study.","authors":"Renan Trevisan Jost, Leonardo Seligra Lopes, Pedro Caetano Edler Zandoná, Jovânio Fernandes da Rosa, Pedro Fernandes Lessa, Felipe Placco Araujo Glina, Nívio Pascoal Teixeira, Sidney Glina","doi":"10.1111/andr.70151","DOIUrl":"10.1111/andr.70151","url":null,"abstract":"<p><strong>Background: </strong>Peyronie's disease (PD) with severe penile curvature often requires surgical correction through plaque incision and grafting (PIG).</p><p><strong>Objectives: </strong>To compare surgical outcomes between polyglycolic acid polymer grafts and porcine small intestinal submucosa (SIS) grafts in patients undergoing PIG for PD.</p><p><strong>Materials and methods: </strong>This retrospective cohort study included patients with PD who underwent PIG using either Gore Bio-A (BioA group, n = 20) or SIS grafts (Cook Biotech, West Lafayette, IN, USA; SIS group, n = 21). The primary outcome was the incidence of postoperative erectile dysfunction (ED). Secondary outcomes included the degree of penile curvature correction, sensory disturbances, patient satisfaction, and surgical complications.</p><p><strong>Results: </strong>The median age was 61 years in the BioA group and 57 years in the SIS group (p = 0.381). The BioA group exhibited a significantly higher rate of refractory ED compared to the SIS group (p = 0.006). No significant differences were observed between groups in terms of penile straightening, sensory alterations, postoperative complications, or the need for penile prosthesis implantation. Patient satisfaction was significantly higher in the SIS group (p = 0.015).</p><p><strong>Discussion: </strong>This study is the first to directly compare Gore Bio-A and SIS grafts in PIG surgery for PD. Despite similar safety profiles and high curvature correction rates in both groups, erectile function outcomes were significantly better in the SIS group. Patient-reported satisfaction also favored SIS. Although associated with higher rates of ED, Gore Bio-A may still represent a viable option in selected cases, particularly when PIG is performed in conjunction with penile prosthesis implantation. Limitations include the retrospective, non-randomized design, and small sample size.</p><p><strong>Conclusion: </strong>In this cohort, the SIS graft was associated with better postoperative outcomes than the BioA graft, with lower rates of refractory ED and higher patient satisfaction following PIG for PD.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2160-2165"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145595695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Three-Step Protocol for Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia Patients.","authors":"Xuefeng Luo, Chen Luo, Chengchao Du, Bo Liu, Xiao Liu, Lijuan Ying, Jinyan Xu, Yuan Liu, Siyu Long, Yang Xian, Fuping Li","doi":"10.1111/andr.70170","DOIUrl":"10.1111/andr.70170","url":null,"abstract":"<p><strong>Background: </strong>Many andrologists put more attention on the predictive factors of successful sperm extraction in testicular sperm aspiration (TESA) or microdissection testicular sperm extraction (micro-TESE), while the importance of laboratory processing of testicular tissue has been neglected. In fact, for non-obstructive azoospermia (NOA) patients, laboratory procedure of testicular tissue is as important as the surgery itself to find testicular sperm, yet there is no standard procedure for laboratory management of testicular tissue.</p><p><strong>Objectives: </strong>To establish a laboratory processing for testicular tissue, and to further evaluate the effectiveness of enzymatic digestion and staining for NOA patients with no spermatozoa found after mechanical mincing.</p><p><strong>Materials and methods: </strong>We enrolled 811 patients who underwent TESA or micro-TESE from July 2023 to December 2024, among whom 466 were diagnosed with obstructive azoospermia and 345 were diagnosed with NOA. The main outcome measure was the sperm retrieval rate (SRR) after enzymatic digestion and the sperm detection rate (SDR) after staining.</p><p><strong>Results: </strong>In this study, testicular spermatozoa was found after mincing in 466 TESA patients (466/583, 79.9%) and 74 micro-TESE patients (74/228, 32.5%). Of the 237 patients for whom spermatozoa were not identified after mincing, spermatozoa were detected in 55 (23.21%) patients after enzyme digestion and staining. The additional SRR contributed by enzymatic digestion alone was 13.1%, while staining contributed an additional SDR of 10.1%. Using multivariable logistic regression analysis, the seminiferous tubule diameter was the only predictor of identifying spermatozoa in NOA patients after enzymatic digestion and staining.</p><p><strong>Discussion and conclusion: </strong>In conclusion, we establish a three-step laboratory processing method for testicular tissue. This study provides strong evidence that enzymatic digestion improves SRR in NOA patients, while staining contributes additional detection value (SDR) when mechanical processing fails, the combined additional value of enzymatic digestion and staining is 23.2%, although only enzymatic digestion contributes to SRR. What is more, the current study showed that seminiferous tubule diameter is a powerful predictor for sperm retrieval, offering real-time, actionable intraoperative guidance. However, the single-center design and lack of live birth outcome data limit the external validity and generalizability of this study.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2180-2188"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nut Consumption and Sperm Quality in Healthy Men: Results From the Led-Fertyl Study.","authors":"Estefanía Dávila-Córdova, Nancy Babio, Cristina Valle-Hita, María Fernández de la Puente, Alfonso Beltran-Arasa, Miguel Cebrián-Puig, Violeta Fambuena-Perez, Iván García-Serrano, Michelle M Murphy, Jordi Salas-Salvadó, Albert Salas-Huetos","doi":"10.1111/andr.70204","DOIUrl":"10.1111/andr.70204","url":null,"abstract":"<p><strong>Background: </strong>Nuts are rich in antioxidants and other bioactive compounds, and recent evidence suggests that their regular consumption may be associated with sperm quality. However, the current scientific evidence remains limited and inconsistent.</p><p><strong>Objective: </strong>The study aimed to evaluate the association between nut consumption and sperm quality parameters in healthy men of reproductive age.</p><p><strong>Materials and methods: </strong>A cross-sectional analysis was conducted using the data from 222 young men enrolled in the Led-Fertyl study. Nut consumption was categorized as < 3, ≥ 3 to < 7, and ≥ 7 servings/week (1 serving = 30 g). The main outcomes were sperm quality parameters (sperm count, concentration, vitality, motility, and normal morphology). Multivariate linear and logistic regression models were fitted to analyze associations.</p><p><strong>Results: </strong>Total sperm count (β = 3.38; 95%CI: 1.59, 5.16) and concentration (β = 1.17; 95%CI: 0.15, 2.19) were higher among participants in the highest category of nut consumption (≥7 servings/week) compared to those in the lowest (< 3 servings/week). A similar association was observed when modeling nut consumption as continuous; each additional serving per day was associated with higher total sperm count and concentration (β = 2.38; 95%CI: 1.03, 3.72 and β = 0.83; 95%CI: 0.06, 1.59, respectively). A theoretical substitution of 1 serving/day of nuts with 1 serving/day of potato chips or pastries was associated with lower total sperm count and concentration. Furthermore, compared to participants in the lowest category of nut consumption, those in the highest were 75% less likely to have abnormal sperm motility (OR: 0.25; 95%CI: 0.07, 0.95) and 69% less likely to have seminogram abnormalities (OR: 0.31; 95%CI: 0.14, 0.68).</p><p><strong>Conclusions: </strong>Our findings suggest that regular nut consumption is associated with higher total sperm count and concentration in young, healthy men of reproductive age.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2262-2273"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13592052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatially Heterogeneous Impairment of Spermatogenesis in the Testis Following Efferent Duct Transection in Mice.","authors":"Hiroki Nakata, Kannika Adthapanyawanich, Yutthapong Tongpob, Wai Chen, Shoichi Iseki, Atsushi Mizokami","doi":"10.1111/andr.70214","DOIUrl":"10.1111/andr.70214","url":null,"abstract":"<p><strong>Background: </strong>Obstructive azoospermia, with a sustained elevation in intratesticular pressure, leads to seminiferous epithelium degeneration. However, studies using efferent duct ligation have not fully elucidated the chronic changes in the testis caused by obstruction.</p><p><strong>Objective: </strong>Using the efferent duct transection model, we aimed to elucidate the temporal and spatial progression of spermatogenic impairment in the mouse testis.</p><p><strong>Materials and methods: </strong>Male mice underwent efferent duct transection and were analyzed at five time points up to 20 weeks. A three-dimensional reconstruction of serial testis sections was employed to visualize and quantitate impaired spermatogenesis in the seminiferous tubules.</p><p><strong>Results: </strong>The volumes of the testis, seminiferous tubules, and interstitium showed 2-3-fold increases 1 week post-transection, but normalized by 5 weeks. The volume of the rete testis continued to increase, and was 9.3-fold larger by 20 weeks. The impairment of spermatogenesis in the seminiferous epithelium was progressive until 20 weeks. Three-dimensional mapping revealed marked spatial heterogeneity in impaired spermatogenesis: cranial seminiferous tubules remained highly viable, while the majority of caudal tubules were impaired. Furthermore, areas of severe impairment were identified in tubule regions near the rete testis in all tubules.</p><p><strong>Discussion and conclusions: </strong>The present results suggest that chronic obstructive stress, driven by mechanical stress and/or local ischemia, is dominant in the caudal region and near the rete testis of the mouse testis. This study established a crucial link between obstructive azoospermia and a pathology resembling non-obstructive azoospermia, underscoring the importance of evaluating such spatial vulnerability in the clinical management of obstructive male infertility.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"2280-2289"},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation Disrupts TRIM21-Mediated AKAP3 Ubiquitination and Triggers Stress Granule Formation in Human Sperm.","authors":"Yan Wang, Huizhong Xu, Wenhong Lu, Yinghao Wang, Jin Ma, Chengyao Ma, Xiangming Yan, Longlong Fu, Haitao Lv, Ying Liu","doi":"10.1111/andr.70403","DOIUrl":"https://doi.org/10.1111/andr.70403","url":null,"abstract":"<p><strong>Background: </strong>Sperm cryopreservation causes cryodamage, but the roles of posttranslational modifications (PTMs) and stress granules (SGs) in this process remain unclear.</p><p><strong>Objectives: </strong>To investigate the molecular mechanism of cryodamage via the TRIM21-AKAP3 axis, PTMs, and SGs.</p><p><strong>Materials and methods: </strong>Human sperm were divided into fresh and cryopreserved groups. Global ubiquitinome, phosphoproteome, and acetylome profiling were performed, combined with co-immunoprecipitation (Co-IP), Western blotting, and immunofluorescence for functional validation. Sperm cryopreservation with FAZ-3532 intervention was further conducted to verify SG-mediated injury.</p><p><strong>Results: </strong>Cryopreservation-induced SG formation in mature human sperm is reported here for the first time. TRIM21 acted as the E3 ubiquitin ligase for AKAP3, targeting K31/K425; cryopreservation redirected TRIM21 into SGs, reducing AKAP3 ubiquitination and causing its accumulation. AKAP3 showed differential phosphorylation (22 sites) and acetylation (K433). Inhibiting SG assembly with FAZ-3532 remodeled AKAP3 ubiquitination patterns and partially improved post-thaw progressive motility.</p><p><strong>Discussion and conclusion: </strong>PTM networks and SGs are critical for sperm cryodamage. TRIM21, AKAP3, and G3BP1 are potential targets to optimize sperm cryopreservation protocols, advancing male fertility preservation.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}