Animal Science Journal最新文献_第6页

Differential Taurine Profiles Reflect Concentrate-Driven Physiological Adaptations in Japanese Black Steers During the Middle Fattening Stage. 不同的牛磺酸谱反映了日本黑阉牛在育肥中期集中驱动的生理适应。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70174

Huseong Lee, Shuntaro Takai, Minji Kim, Shinichiro Torii, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Itoko Nonaka, Akane Ashihara, Oh Kyunghwan, Yoshinobu Uemoto, Fuminori Terada, Satoshi Haga, Sanggun Roh

{"title":"Differential Taurine Profiles Reflect Concentrate-Driven Physiological Adaptations in Japanese Black Steers During the Middle Fattening Stage.","authors":"Huseong Lee, Shuntaro Takai, Minji Kim, Shinichiro Torii, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Itoko Nonaka, Akane Ashihara, Oh Kyunghwan, Yoshinobu Uemoto, Fuminori Terada, Satoshi Haga, Sanggun Roh","doi":"10.1111/asj.70174","DOIUrl":"10.1111/asj.70174","url":null,"abstract":"Taurine, a sulfur-containing amino acid derived from methionine or cysteine, plays key roles in bile acid conjugation, antioxidant defense, and lipid and glucose metabolism. However, its relationship with physiological and metabolic changes in Japanese Black cattle, especially during the middle fattening stage, when plasma taurine rises with increased concentrate feeding, remains unclear. This study aimed to clarify the association between plasma taurine concentration and metabolic as well as ruminal characteristics during the middle fattening stage in Japanese Black steers (Bos taurus). Among the 21 steers, the highest (TauH, n = 6) and lowest (TauL, n = 6) steers in plasma taurine concentration were selected for comparison and their growth performance, blood metabolites, and rumen fermentation profiles were compared. The TauH group showed higher concentrate intake, greater ruminal propionate production, superior growth performance, and elevated plasma insulin, cholesterol, phospholipid, and aspartic acid concentrations than the TauL group. KEGG Orthology analysis indicated that methionine biosynthesis was enhanced in the rumen microbiota of TauH steers, whereas cysteine-related pathways predominated in TauL animals. Plasma taurine concentration closely reflected concentrate-driven metabolic adaptation, indicating that it represents a practical biomarker for assessing nutritional status and refining feeding strategies to improve growth and metabolic efficiency in beef cattle.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70174"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy Balance Prediction in Japanese Holstein Cows by Using Milk Fatty Acid Composition. 利用乳脂肪酸组成预测日本荷斯坦奶牛能量平衡。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70192

Akiko Nishiura, Osamu Sasaki, Tamako Tanigawa, Yuriko Saito, Ryoki Tatebayashi

{"title":"Energy Balance Prediction in Japanese Holstein Cows by Using Milk Fatty Acid Composition.","authors":"Akiko Nishiura, Osamu Sasaki, Tamako Tanigawa, Yuriko Saito, Ryoki Tatebayashi","doi":"10.1111/asj.70192","DOIUrl":"10.1111/asj.70192","url":null,"abstract":"Negative energy balance (NEB) in early lactation adversely affects health and fertility in dairy cows, yet direct measurement of energy balance (EB) requires intensive monitoring of individual feed intake. We developed a practical EB prediction equation by augmenting routine milk yield and milk composition data with milk fatty acid (FA) information-specifically de novo and preformed FA proportions-and evaluated its external validity. By using 14,993 records from 93 Holstein cows from the Hokkaido Research Organization (6-103 days in milk [DIM]), stepwise regression based on the Schwarz Bayesian Criterion selected six traits: preformed FA, milk fat percentage, standardized DIM class, de novo FA, milk yield, and milk protein percentage. The resulting model achieved an R2 of 0.65. External validation by using nationwide data from 18 institutions yielded an R2 of 0.32-higher than that of our previous equation (R2 = 0.17). Preformed FA, reflecting body fat mobilization, contributed most strongly to EB prediction, whereas de novo FA added complementary physiological information. Compared with equations based on milk yield and milk composition alone, incorporating FTIR-predicted milk FA composition improves the accuracy and robustness of EB prediction across diverse management conditions, supporting its application in metabolic monitoring and genetic evaluation.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70192"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147980245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of Plasma Glucagon-Like Peptide 1 Concentration to Oral Administration of Lactose and Casein in Preweaning Calves. 断奶前犊牛血浆胰高血糖素样肽1浓度对口服乳糖和酪蛋白的响应。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70187

Yudai Inabu, Midori Satoh, Taketo Obitsu, Toshihisa Sugino

{"title":"Response of Plasma Glucagon-Like Peptide 1 Concentration to Oral Administration of Lactose and Casein in Preweaning Calves.","authors":"Yudai Inabu, Midori Satoh, Taketo Obitsu, Toshihisa Sugino","doi":"10.1111/asj.70187","DOIUrl":"10.1111/asj.70187","url":null,"abstract":"This study investigated the effects of orally administered sugars and casein, main components of whole milk, on plasma glucagon-like peptide 1 (GLP-1) concentration in preweaning calves. In Experiment 1, calves received water (CON), 2.37% (w/w) glucose (GLU), 2.37% galactose (GAL), or a mixed solution containing 2.37% each of glucose and galactose (GLU + GAL). In Experiment 2, calves received 4.5% (w/w) lactose (LAC), 3.0% casein (CAS), or a mixed solution containing lactose at 4.5% and casein at 3.0% (LAC + CAS). Because baseline plasma GLP-1 and α-amino nitrogen (AAN) concentrations varied greatly and correlated with postadministration mean concentrations, statistical analyses for these parameters included baseline values as covariates. In Experiment 1, glucose and insulin concentrations were higher in GLU and GLU + GAL than in CON (p < 0.001), whereas GLP-1 concentrations were lower in GAL than in other groups (p < 0.001). In Experiment 2, glucose and insulin concentrations were higher in LAC and LAC + CAS, AAN concentrations were higher in CAS and LAC + CAS, and GLP-1 concentrations were higher in LAC, CAS, and LAC + CAS compared with CON (p < 0.001). In conclusion, both lactose and casein promoted GLP-1 secretion in calves, although oral administration of glucose or galactose alone did not elicit a marked GLP-1 response.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70187"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardized Ileal Digestibility of Amino Acids in L-Amino Acids Sources and Single Cell Protein Biomass Coproduct for Pigs. 猪l -氨基酸源氨基酸的标准化回肠消化率和单细胞蛋白质生物量副产物。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70177

Stephane Alverina Briguente da Motta, Nathana Rudio Furlani, Antônio Carlos Macedo Lourenço, Pedro Silva Carelli, Lucimauro da Fonseca, Bruno Teixeira Ramos, Gabriel Ribeiro Braga, Melissa Izabel Hannas

{"title":"Standardized Ileal Digestibility of Amino Acids in L-Amino Acids Sources and Single Cell Protein Biomass Coproduct for Pigs.","authors":"Stephane Alverina Briguente da Motta, Nathana Rudio Furlani, Antônio Carlos Macedo Lourenço, Pedro Silva Carelli, Lucimauro da Fonseca, Bruno Teixeira Ramos, Gabriel Ribeiro Braga, Melissa Izabel Hannas","doi":"10.1111/asj.70177","DOIUrl":"10.1111/asj.70177","url":null,"abstract":"Nine male pigs, which average 44.8 ± 3.6 kg body weight, were surgically fitted with a T cannula in the terminal ileum of the small intestine. The animals were assigned to a triplicated 3 × 2 incomplete Latin square design, consisting of three treatments: a complete mixture of industrial amino acids (L-AAs), a single cell protein (SCP) biomass coproduct, and an nitrogen-free diet. The animals were fed twice a day, with 5 days of adaptation to the experimental diets, followed by 2 days of continuous collection of ileal digesta for 10 h. Titanium dioxide was included in all diets at 0.5% as an indigestibility marker, and the N-free diet was used to estimate basal endogenous ileal losses of amino acids (AA). The standardized ileal digestibility (SID) of crude protein was higher (p < 0.05) for L-AAs compared to SCP (101.3% vs. 73.4%). For the indispensable and dispensable AA, the L-AAs also presented higher SID (p < 0.05) to SCP. Specifically for L-tryptophan, the SID of SCP was 89.2%. These results highlight that L-AAs are highly digestible, with SID values close to 100%, whereas SCP demonstrates viability as a source of indispensable and dispensable AA in swine nutrition.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70177"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Cellulase and Lactobacillus plantarum on the Quality, Microbial Composition, and Rumen Degradation Characteristics of Apple Pomace and Wheat Bran Mixed Silage. 纤维素酶和植物乳杆菌对苹果渣-麦麸混合青贮品质、微生物组成及瘤胃降解特性的影响

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70212

Xiongfei Zhang, Lixuan Wu, Jingtao Hui, Qilin Wang, Zhuangzhuang Liu, Fengbo Sun, Junhu Yao, Xinjian Lei

{"title":"Effect of Cellulase and Lactobacillus plantarum on the Quality, Microbial Composition, and Rumen Degradation Characteristics of Apple Pomace and Wheat Bran Mixed Silage.","authors":"Xiongfei Zhang, Lixuan Wu, Jingtao Hui, Qilin Wang, Zhuangzhuang Liu, Fengbo Sun, Junhu Yao, Xinjian Lei","doi":"10.1111/asj.70212","DOIUrl":"10.1111/asj.70212","url":null,"abstract":"The objective of this study was to evaluate the individual effects of cellulase and Lactobacillus plantarum, as well as their interaction effect on the fermentation quality, microbial composition, and rumen degradation characteristics of apple pomace (AP) and wheat bran mixed silage. The experiment used a 2 × 2 factorial design with two factors: cellulase and L. plantarum, each with two levels (added or not added). The results demonstrated that cellulase supplementation in AP and wheat bran mixed silage increased acetic acid and total organic acid (TOA) levels while decreasing pH. Cellulase addition reduced neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents, increased water-soluble carbohydrate concentration, but elevated dry matter (DM) loss rate. Regarding rumen degradation characteristics, cellulase addition enhanced the effective rumen degradability of DM but reduced the effective rumen degradability of NDF and ADF. L. plantarum inoculation increased lactic acid and TOA levels, reduced pH and DM loss, and promoted a higher effective rumen degradation rate of NDF and ADF. Combined supplementation with cellulase and L. plantarum enhanced the relative abundance of lactic acid bacteria and the content of LA, reduced pH, NDF, and ADF, and increased ruminal effective degradability of nutrients. In conclusion, the combined supplementation of cellulase and L. plantarum exhibited a partial synergistic effect. The combined supplementation of cellulase and L. plantarum exerted a partial synergistic effect, preventing the increase in DM loss rate and the decrease in ruminal effective degradability of ADF induced by cellulase alone.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70212"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13349430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modified Zymography Protocol: A Rapid and Sensitive Method for Monitoring Calpain Activity. 改良酶谱法：一种快速灵敏的钙蛋白酶活性监测方法。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70237

Onkar Sharma, Aaditya Narayan Pathak, Nirmal Jeet Kaur, Nandini, Anita Dua, Sanjeev Gupta, Ashwani Mittal

{"title":"Modified Zymography Protocol: A Rapid and Sensitive Method for Monitoring Calpain Activity.","authors":"Onkar Sharma, Aaditya Narayan Pathak, Nirmal Jeet Kaur, Nandini, Anita Dua, Sanjeev Gupta, Ashwani Mittal","doi":"10.1111/asj.70237","DOIUrl":"10.1111/asj.70237","url":null,"abstract":"Calpain is a key proteolytic enzyme involved in muscle development. Overactivation in diseases can lead to muscle atrophy, and its role in postmortem meat tenderization affects consumer acceptability. To develop a rapid and sensitive zymography protocol for the efficient detection of calpain activity in skeletal muscle cells, we addressed the limitations of traditional methods that require lengthy assay times and high protein inputs. A previously published zymography method was modified by increasing the Ca2+ concentration from 4 to 8 mM and reducing the incubation time from overnight to 6 h. Calpain activity was assessed in differentiated C2C12 myotubes using varying protein concentrations (1-8 μg), and band intensity was quantified using ImageJ software. The optimized conditions enhanced the detection sensitivity up to tenfold and enabled reliable visualization of calpain activity using only 1 μg of protein within 8 h. The 8 mM Ca2+ incubation buffer yielded stronger, clearer lytic bands compared with 4 mM, indicating improved calpain activation. This modified zymography protocol significantly reduces assay time and protein requirement while maintaining high sensitivity. The method serves as an efficient tool for studying calpain-mediated proteolysis in muscle atrophy research and postmortem meat quality evaluation.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70237"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13525210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of in Ovo Feeding With Glycosaminoglycans and Vitamins on Intestinal Morphometry and Cadherin-1 Gene Copy Abundance in Broilers. 饲粮中添加糖胺聚糖和维生素对肉仔鸡肠道形态和钙粘蛋白-1基因拷贝丰度的影响。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70186

Elaine Talita Santos, Silvana Martinez Baraldi-Artoni, Jean Kaique Valentim, Rodrigo Garófallo Garcia, Peter Ferket, Ramon Diniz Malheiros, Melody Martins Cavalcante Pereira, Sarah Sgavioli

{"title":"Effects of in Ovo Feeding With Glycosaminoglycans and Vitamins on Intestinal Morphometry and Cadherin-1 Gene Copy Abundance in Broilers.","authors":"Elaine Talita Santos, Silvana Martinez Baraldi-Artoni, Jean Kaique Valentim, Rodrigo Garófallo Garcia, Peter Ferket, Ramon Diniz Malheiros, Melody Martins Cavalcante Pereira, Sarah Sgavioli","doi":"10.1111/asj.70186","DOIUrl":"10.1111/asj.70186","url":null,"abstract":"This study evaluated the effects of in ovo feeding with glycosaminoglycans and vitamins, combined with amphotericin and gentamicin, on incubation traits, intestinal morphometry, organ development, and cadherin-1 gene relative abundance in broilers. Fertile Ross 308 breeder eggs were randomly assigned to the following five treatments: uninjected eggs; eggs injected with a solution containing amphotericin, gentamicin, ascorbic acid, and pyridoxine; the same solution supplemented with chondroitin sulfate; glucosamine sulfate; or both glycosaminoglycans. Hatching rate, embryo mortality, and total mortality were not affected by treatments (p > 0.05). Broilers from the solution-based treatment showed higher relative heart weight at 1 day of age (p < 0.05). Intestinal morphometric parameters increased with age in both the jejunum and ileum. In ovo feeding modulated villus height, crypt depth, and villus height-crypt depth ratio, particularly in the ileum during early posthatch development. The relative abundance of the cadherin-1 (CDH1) gene was influenced by the interaction between in ovo feeding and age, with higher values observed in broilers receiving glycosaminoglycan-supplemented solutions at specific posthatch periods. In conclusion, in ovo feeding with glycosaminoglycans and vitamins modulated early intestinal development and relative heart weight without impairing hatchability in broilers.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70186"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Different Treatment on the Chemical Composition, Ruminal Degradation Kinetics and In Vitro Digestibility of Cottonseed. 不同处理对棉籽化学成分、瘤胃降解动力学及体外消化率的影响。

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70243

Mamak Rezaeian Tabrizi, Seyyed Roohollah Ebrahimi-Mahmoudabad, Amir Fattah

{"title":"Effects of Different Treatment on the Chemical Composition, Ruminal Degradation Kinetics and In Vitro Digestibility of Cottonseed.","authors":"Mamak Rezaeian Tabrizi, Seyyed Roohollah Ebrahimi-Mahmoudabad, Amir Fattah","doi":"10.1111/asj.70243","DOIUrl":"https://doi.org/10.1111/asj.70243","url":null,"abstract":"This study evaluated the effects of gamma ray (GR), electron-beam (EB) irradiation, microwave irradiation (MI) and roasting (R) on the chemical composition, ruminal degradability and in vitro digestibility of whole cottonseed (CS). Cottonseed samples were treated with gamma irradiation (20 and 40 kGy), electron beam (20 and 40 kGy), microwave irradiation (3 and 5 min), and roasting (at 140°C for 15 and 30 min). Chemical composition was analyzed by standard methods, whereas ruminal degradability of dry matter (DM) and crude protein (CP) was measured using the nylon bag technique with three fistulated cows. In vitro digestibility was determined with the Tilley and Terry method, and amino acid degradability was assessed across treatments. All processing methods decreased neutral detergent fiber (NDF) and acid detergent fiber (ADF) of CS compared to untreated CS (p < 0.05). Gamma and microwave irradiation decreased DM and CP degradability of CS at ruminal outflow rates of 0.02, 0.05 and 0.08 h-1 (p < 0.05). All treatments decreased effective rumen degradability (ERD) of CS at ruminal out flow rates of 0.02, 0.05 and 0.08 h-1 (p < 0.05). Roasting and microwave irradiation for 5 min decreased water soluble fraction and increased the slowly degradable fraction of CS compared to untreated CS (p < 0.05). In vitro digestibility of 40 kGy irradiated CS and roasted CS for 30 min was significantly higher than other treatments and untreated CS (p < 0.05). Ruminal disappearances of essential and non-essential amino acids after 16 h incubation in rumen were decreased by electron beam irradiation (p < 0.05). These results indicate that physical processing methods, especially electron beam and roasting, enhance nutritional value of CS for ruminants.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70243"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the Switch From a Short-Day Photoperiod With Natural Light to a Long-Day Photoperiod With White Light-Emitting Diode or Induction Lighting on Milk Production and Endocrine Profiles in Lactating Dairy Cows. 短日自然光周期切换到长日白光二极管或感应光周期对泌乳奶牛产奶量和内分泌的影响

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70245

Yudai Inabu, Yusuke Takakura, Yoshitada Shinohara, Mitsutoshi Sunadome, Ryunosuke Watanabe, Shiro Kushibiki, Toshihisa Sugino

{"title":"Effects of the Switch From a Short-Day Photoperiod With Natural Light to a Long-Day Photoperiod With White Light-Emitting Diode or Induction Lighting on Milk Production and Endocrine Profiles in Lactating Dairy Cows.","authors":"Yudai Inabu, Yusuke Takakura, Yoshitada Shinohara, Mitsutoshi Sunadome, Ryunosuke Watanabe, Shiro Kushibiki, Toshihisa Sugino","doi":"10.1111/asj.70245","DOIUrl":"10.1111/asj.70245","url":null,"abstract":"This study evaluated the response of milk production and endocrine profile to a switch from short-day photoperiod (SDPP) with natural light to long-day photoperiod (LDPP) with white light-emitting diode (WLED) or induction lighting (IL) in lactating dairy cows. Nine Holstein cows (198 ± 10.0 days in milk) were initially exposed to SDPP of 10:14 h light-dark cycle (10L:14D) with natural light (SDPP period), followed by a long-day photoperiod (LDPP; 16L:8D) using either WLED (443 nm peak, LDPP-W, n = 5) or IL (IL; 529 nm peak, LDPP-I, n = 4). Each period comprised a 1-week adaptation and a 2-week sampling period. Milk yield decreased after switching to LDPP-W (p < 0.05) but remained unchanged with LDPP-I. The slope of milk yield over the entire trial period (from the beginning of SDPP to the end of LDPP period) differed depending on lighting type (p = 0.05), being negative in the LDPP-W and positive in the LDPP-I. Plasma prolactin concentration in the light period increased after switching to LDPP-I but not to LDPP-W. These results indicate that LDPP using IL is likely more effective in promoting milk yield than WLED, potentially due to enhanced prolactin secretion.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70245"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13562985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148928777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ssc-miR-130b Inhibits Lipid Accumulation in Porcine Intramuscular Preadipocytes by Activating Apoptosis via Bcl-2/Bax/Caspase-3 Signaling Pathway. Ssc-miR-130b通过Bcl-2/Bax/Caspase-3信号通路激活凋亡抑制猪肌内前脂肪细胞脂质积累

IF 1.6 3区农林科学

Animal Science Journal Pub Date : 2026-01-01 DOI: 10.1111/asj.70164

Lulu Li, Yongfang Chen, Lijun Wang, Ji Cheng, Yao Lu, Shifeng Pan

{"title":"Ssc-miR-130b Inhibits Lipid Accumulation in Porcine Intramuscular Preadipocytes by Activating Apoptosis via Bcl-2/Bax/Caspase-3 Signaling Pathway.","authors":"Lulu Li, Yongfang Chen, Lijun Wang, Ji Cheng, Yao Lu, Shifeng Pan","doi":"10.1111/asj.70164","DOIUrl":"10.1111/asj.70164","url":null,"abstract":"MicroRNAs (miRNAs) play pivotal roles in intramuscular fat (IMF) deposition and determine animal meat quality. The study aimed to investigate the effect of miR-130b on adipogenesis in porcine intramuscular preadipocytes (PIMPA). In the study, PIMPA were isolated from longissimus dorsi muscle and cultured until 85%-90% confluence. The cells then subjected to adipogenic induction and transient transfections with miR-130b mimic, miR-130b inhibitor, and their corresponding controls (mNC and iNC). Oil red O staining was performed to detect lipid accumulation. Differentially expressed (DE) mRNAs were screened by transcriptome sequencing, and enriched functional pathways were identified through GO and KEGG analysis. The results demonstrated that numerous DE mRNAs were identified between the miR-130b mimic and mNC groups, which were mainly enriched in apoptosis, autophagy, and AMPK signaling pathways. Mechanistically, miR-130b overexpression significantly increased Bax expression, while reducing caspase-3 and Bcl-2 expression as well as Bcl-2/Bax ratio. It also, and tended to increase cleaved caspase-3/caspase-3 ratio, thereby activating apoptosis and suppressing lipid accumulation. Furthermore, these above results can be significantly reversed by miR-130b inhibition. In conclusion, our findings highlight that miR-130b inhibits lipid accumulation by activating apoptosis via Bcl-2/Bax/caspase-3 signaling pathway in PIMPA, providing a reliable molecular target for improving animal meat quality and ameliorating human obesity.","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":"97 1","pages":"e70164"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0