Animal Reproduction Science最新文献

{"title":"A new urinary biomarker reflecting the progesterone excretion pattern identified through serum steroid and urinary progesterone metabolite analysis during pro-oestrus and oestrus in female dogs (Canis familiaris)","authors":"Tia M. Pennanen ,&nbsp;Merja R. Häkkinen ,&nbsp;Risto O. Juvonen ,&nbsp;Jouko J. Vepsäläinen ,&nbsp;Seppo Auriola ,&nbsp;Sari M. Viitala","doi":"10.1016/j.anireprosci.2025.108054","DOIUrl":"10.1016/j.anireprosci.2025.108054","url":null,"abstract":"<div><div>In female dogs, the optimal breeding time is estimated by an increase in the serum progesterone hormone concentration. The method is not only invasive and unpleasant to dogs but also laborious and requires professional laboratory expertise. The main aim of this study was to explore potential urinary progesterone metabolites for non-invasive oestrus monitoring. Additionally, thirteen steroid hormones, such as sex steroids and corticosteroids, were determined in serum samples from nine female dogs via multiple reaction monitoring liquid chromatography<img>mass spectrometry (LC<img>MS). In addition, various urinary steroid metabolites from 22 dogs were analysed via nontargeted liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis. The serum oestrone, oestradiol, and progesterone concentration profiles were aligned with the existing literature. When the 17β-oestradiol secretion peak (49–83 pg/mL) during pro-oestrus was considered as Day 0, 17α-hydroxyprogesterone, testosterone, and androstenedione concentrations peaked on Days 0–3 (<em>P</em>-values 0.004, 0.015 and 0.007, respectively). Results of corticosteroids unveiled novel insights into steroid secretion during the breeding season of dogs. Parallel urine samples revealed that progesterone was excreted into the urine as the unidentified pregnanetriol glucuronide “PtGX” instead of the common metabolite pregnanediol-3-glucuronide (PdG) among mammals. The excretion profiles of pregnanetriol glucuronide support its usability as a biomarker in non-invasive oestrus monitoring in female dogs. The findings presented herein lay the foundation for a more animal-friendly and streamlined approach to monitoring the oestrus cycle of dogs, with substantial implications for dog breeding and advancing animal welfare practices.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"284 ","pages":"Article 108054"},"PeriodicalIF":3.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of post-mating TAK-683 (Kisspeptin analog) treatment on luteal morphology and function in suckling goats with lactational anestrus","authors":"Gökhan Uyanık ,&nbsp;Murat Yüksel ,&nbsp;Murat Abay ,&nbsp;Ahmet Gözer ,&nbsp;Ufuk Kaya ,&nbsp;Filiz Kara ,&nbsp;Ebru Arslanhan ,&nbsp;Ishak Gökçek","doi":"10.1016/j.anireprosci.2025.108052","DOIUrl":"10.1016/j.anireprosci.2025.108052","url":null,"abstract":"<div><div>This study aimed to evaluate the effects of TAK-683 administered to suckling goats in lactational anestrus on the 4th day (D4) after mating on luteal function, serum progesterone (P₄) levels, and reproductive performance. Forty-five Aleppo goats were treated with intravaginal sponges containing medroxyprogesterone acetate for 7 days and were injected intramuscularly with 500 IU eCG and 75 µg <em>d</em>-cloprostenol on the day of sponge removal. Thirty-seven goats showed estrus and were mated with fertile bucks (D0). The mated goats were randomly divided into groups to receive subcutaneous administration of saline (Control, <em>n</em> = 12), 5 μg TAK-683 (TAK-5, <em>n</em> = 12) or 25 μg TAK-683 (TAK-25, <em>n</em> = 13) on D4. Transrectal ovarian ultrasonography and blood sampling were performed on D4, D7, D14, D17, and D21, and pregnancy was diagnosed on D35. Regardless of dose, TAK-683 treatment induced the formation of accessory corpora lutea (aCL) and significantly increased original CL (oCL) diameter, luteal area (LA), and Doppler area (DA) compared with controls (<em>P</em> &lt; 0.001). Total LA and DA were greater (<em>P</em> &lt; 0.001) in TAK-5 and TAK-25, although DA/LA ratio did not differ (<em>P</em> &gt; 0.05). Serum P₄ levels were significantly higher in TAK-25 from D7 to D21 (<em>P</em> &lt; 0.001), while TAK-5 showed a delayed increase from D17. Luteal morphological parameters were strongly and positively correlated with circulating P₄ levels, confirming the luteotropic effect of TAK-683. TAK-683 treatment had no significant effect on fertility parameters (<em>P</em> &lt; 0.05). In conclusion, although TAK-683 treatment enhanced luteal function and increased circulating P₄ concentrations, it failed to improve fertility outcomes in suckling goats under lactational anestrus conditions.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"284 ","pages":"Article 108052"},"PeriodicalIF":3.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estradiol esters are dispensable in progesterone-based TAI protocols for Bos taurus suckling beef cows","authors":"Alinne Machado Petrarca Léo ,&nbsp;Fabiane Pereira de Moraes ,&nbsp;Monique Tomazele Rovani ,&nbsp;Rogério Ferreira ,&nbsp;José Nélio de Sousa Sales ,&nbsp;Rafael Gianella Mondadori ,&nbsp;Bernardo Garziera Gasperin","doi":"10.1016/j.anireprosci.2025.108050","DOIUrl":"10.1016/j.anireprosci.2025.108050","url":null,"abstract":"<div><div>Timed artificial insemination (TAI) protocols in South America are mainly based on progesterone (P4) and estradiol (E2). Considering the restrictions on E2 use, the study aimed to evaluate its replacement with GnRH. Multiparous suckling <em>Bos taurus</em> cows (<em>n</em> = 455) were pre-synchronized and received a P4 (1 g) intravaginal device (IVD) on D0. Also, on D0, cows in the Control group received 2 mg of estradiol benzoate IM and cows in the GnRH/GnRH and GnRH/EC groups received 20 µg of buserelin acetate IM. At IVD removal (D7), all cows received 500 µg sodium cloprostenol IM, 300IU eCG IM, and tail paint. At this time, cows in the Control and GnRH/EC groups received 1 mg estradiol cypionate (EC) IM. TAI was performed on D9 (48 h after IVD removal) in all cows, and 10 µg of buserelin acetate IM was administered only in GnRH/GnRH group. No difference was observed among Control (<em>n</em> = 85), GnRH/EC (<em>n</em> = 82) and GnRH/GnRH (<em>n</em> = 84) regarding largest follicle (LF) diameter on D7 (7.3 ± 0.2, 7.6 ± 0.3 and 7.6 ± 0.3 mm, respectively; <em>P</em> = 0.75) and percentage of CL on D7 (87.1, 80.5 and 81.0 %. respectively; <em>P</em> = 0.37). Significant effects of D7 LF diameter (<em>P</em> &lt; 0.0001; odds ratio 1.58) and D7 LF by group interaction on pregnancy rate were observed (<em>P</em> &lt; 0.01). No difference was observed in the estrus rate before TAI (D9; 72.1, 62.4 and 67.9 %; <em>P</em> = 0.22) or in the pregnancy rate (56.9, 53.1 and 53.6 %; <em>P</em> = 0.77) for control (<em>n</em> = 153), GnRH/EC (<em>n</em> = 147) and GnRH/GnRH (<em>n</em> = 155), respectively. In conclusion, estradiol replacement by GnRH in TAI protocols does not affect LF diameter, estrus expression and pregnancy rate in suckling <em>Bos taurus</em> beef cows.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"284 ","pages":"Article 108050"},"PeriodicalIF":3.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-23a represses CELF1 to modulate buffalo granulosa cell apoptosis and steroidogenesis via the PI3K/AKT pathway","authors":"Haoxin Wang ,&nbsp;Yun Feng ,&nbsp;Xin Zhao ,&nbsp;Penglei Shen ,&nbsp;Deshun Shi ,&nbsp;Fenghua Lu","doi":"10.1016/j.anireprosci.2025.108040","DOIUrl":"10.1016/j.anireprosci.2025.108040","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are critical regulators of mammalian follicle development. Our previous work showed that miR-23a expression was significantly higher in buffalo oocytes at metaphase II (MII) than at the germinal vesicle (GV) stage, implying a role in oocyte maturation. Here, we investigated the function and mechanism of miR-23a in buffalo granulosa cells (GCs). GCs cultured in vitro were transfected with miR-23a mimics or inhibitor. The miR-23a mimic markedly increased apoptosis, reduced estradiol (E2) release, elevated progesterone (P4) secretion, and suppressed the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway, whereas the inhibitor exerted the opposite effects. Dual-luciferase and rescue assays revealed that miR-23a directly targets CUG triplet repeat-binding protein 1 (<em>CELF1</em>). Overexpression of <em>CELF1</em> reversed the pro-apoptotic effect of miR-23a, restored proliferation, normalized E2 and P4 production, and re-activated PI3K/AKT signaling. Thus, miR-23a targeted <em>CELF1</em> to inhibit the PI3K/AKT pathway, promoting apoptosis, suppressing proliferation and modulating steroidogenesis in buffalo GCs, thereby controlling follicular homeostasis.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108040"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of collagen in ovarian extracellular matrix and its relationship with the development of primordial follicles up to ovulation","authors":"Emanoel da S. Félix,&nbsp;José R.V. Silva","doi":"10.1016/j.anireprosci.2025.108029","DOIUrl":"10.1016/j.anireprosci.2025.108029","url":null,"abstract":"<div><div>The extracellular matrix (ECM) is a dynamic network of proteins and biomolecules that performs essential structural and signaling functions in mammalian ovarian physiology. In addition to modulating processes such as cell adhesion, migration, and differentiation, the ECM is actively involved in regulating tissue stiffness, a physical property directly involved in follicle activation and development. Among its components, collagen fibrils stand out as determinants in maintaining ovarian architecture and remodeling the follicular microenvironment throughout folliculogenesis. Evidence from in vivo and in vitro models shows that changes in the composition, organization, and distribution of collagen fibrils, particularly types I, III, IV, and VI, influence the transition from primordial follicles to growth stages and are involved in the intense remodeling that occurs during ovulation and reproductive senescence. In this review, we discuss the central role of collagen fibrils in regulating ovarian cortex stiffness and its relationship to follicular growth, highlighting cellular and molecular mechanisms associated with ECM remodeling in different species and physiological contexts.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108029"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145450609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotic GABA and Lactobacillus plantarum 1–2-3 alleviate stress-induced ovarian dysfunction and improve reproductive performance in laying hens through multi-mechanistic pathways","authors":"Xi Huang,&nbsp;Zhangshan Gao,&nbsp;Peichao Gao,&nbsp;Helong Ji,&nbsp;Laizhi Xu,&nbsp;Dengfei Feng,&nbsp;Wentao Fan,&nbsp;Suquan Song","doi":"10.1016/j.anireprosci.2025.108026","DOIUrl":"10.1016/j.anireprosci.2025.108026","url":null,"abstract":"<div><div>Stress is a well-established disruptor of reproductive function in poultry. Gamma-aminobutyric acid (GABA), a notable postbiotic derived from <em>Lactobacillus plantarum</em> (<em>L. plantarum</em>), has attracted increasing attention as a dietary supplement. While existing research predominantly focuses on GABA applications in broilers and other species, its effects on laying hens remain underexplored. This study investigated whether GABA and <em>L. plantarum</em> 1–2–3 supplementation mitigated corticosterone (CORT)-induced chronic stress in laying hens. Results demonstrated that both GABA and <em>L. plantarum</em> 1–2–3 effectively mitigated CORT-induced declines in laying performance and ovarian damage. They reduced ovarian oxidative stress, inflammation, and atretic follicles by enhancing antioxidant capacity and normalizing expression of inflammatory factors (<em>IL18</em>, <em>LITAF</em>, <em>IL6</em>, <em>IL1B)</em>, and apoptosis-related factors (<em>BCL2</em>, <em>BAX</em>, <em>CASP9</em>, <em>CASP3</em>). Furthermore, GABA and <em>L. plantarum</em> 1–2–3 markedly increased the serum reproductive hormone levels, as well as the transcription level of ovarian steroidogenic genes (<em>LHCGR, FSHR, StAR, CYP11A1</em>), while modulated ovarian cholesterol metabolism via PPARα-mediated pathways. These findings elucidate the mechanisms by which GABA and <em>L. plantarum</em> 1–2–3 alleviate stress in laying hens, providing a theoretical and practical basis for their use as functional feed additives in stress-exposed flocks.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108026"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145384511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary supplementation with gallnut tannic acid in early pregnancy on reproductive performance and gut microbiota of sows","authors":"Jie Wang ,&nbsp;Guangling Hu ,&nbsp;Qingwu Shi ,&nbsp;Zhijun Luo ,&nbsp;Zhenqing Yang ,&nbsp;Caizai Zhang ,&nbsp;Yiyu Zhang ,&nbsp;Xiaoli Shi ,&nbsp;Zheng Ao","doi":"10.1016/j.anireprosci.2025.108028","DOIUrl":"10.1016/j.anireprosci.2025.108028","url":null,"abstract":"<div><div>The survival of early embryos during the first trimester is closely associated with sow reproductive performance. Embryo development and implantation in this period are accompanied by oxidative stress and inflammation. Gallnut tannic acid (GTA) has demonstrated strong antioxidant and anti-inflammatory properties; however, its effects on sow reproductive performance remain poorly defined. Here, we investigated the effects of dietary GTA supplementation during early gestation (days 7–30) on sow reproductive performance, gut microbiota, and underlying mechanisms. A total of 100 sows (Landrace × Large White) were allocated to four groups (n = 25 per group): a basal control diet (CON) or the basal diet supplemented with 0.05 % (I), 0.1 % (II), or 0.2 % (III) GTA. GTA supplementation tended to increase average litter size (<em>P</em> = 0.068), with the 0.1 % group (II) achieving the highest value (17.05 piglets per litter). GTA enhanced systemic antioxidant capacity by increasing serum total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels, whereas malondialdehyde (MDA). It also significantly reduced serum IL-6 concentrations levels (<em>P</em> &lt; 0.05). <em>In vitro</em>, 6 μM tannic acid (TA) promoted trophoblast cell proliferation and upregulated the expression of implantation-related genes (<em>PLET1</em>, <em>SPP1</em>), antioxidant activity (<em>CAT</em>, <em>CuZnSOD</em>, <em>MnSOD</em>), an angiogenesis marker (<em>VEGFR1</em>), and the proliferation marker <em>PCNA</em>, while modulating inflammatory gene expression in porcine trophoblast (pTr2) cells <em>(P</em> &lt; 0.05). Gut microbiota analysis revealed dose-dependent alterations, with the 0.2 % GTA group (III) showing the most pronounced compositional shifts at both phylum and family levels. In conclusion, dietary GTA supplementation during early gestation may improve sow reproductive performance by enhancing antioxidant capacity, regulating inflammatory homeostasis, and upregulating implantation-related gene expression. The optimal dietary level appears to be approximately 0.1 % GTA.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108028"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145475107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol's therapeutic role in heat stress-induced mitochondria and ER dysfunction in animal reproductive health: A review","authors":"Muhammad Tariq ,&nbsp;Abdul Quddus ,&nbsp;Kebede Habtegiorgis Beshah ,&nbsp;Dagan Mao","doi":"10.1016/j.anireprosci.2025.108044","DOIUrl":"10.1016/j.anireprosci.2025.108044","url":null,"abstract":"<div><div>Heat stress is a major environmental factor that causes oxidative stress, mitochondrial dysfunction, and endoplasmic reticulum (ER) stress, which may lead to fertility impairment, decreased fetal growth, and low survival rates. Resveratrol is an antioxidant polyphenolic compound which has been found to have potential in mitigating the damage caused by heat stress. This review explores the therapeutic role of resveratrol in reducing mitochondrial and ER dysfunction in animal reproduction, focusing on its role in enhancing reproductive performance under heat stress. The systematic analysis of the recent studies demonstrates that resveratrol enhances mitochondrial biogenesis, increases mitochondrial dynamics via balanced fission and fusion, and controls the unfolded protein response (UPR), which reduces ER stress. Moreover, resveratrol triggers the SIRT1/AMPK signal transduction pathway that is involved in cellular stress defense and metabolic stability. This review identifies experimental evidence of resveratrol to protect cells related to reproduction against oxidative damage, maintain mitochondrial integrity, and abate apoptosis. Evidence shows that the antioxidant and anti-inflammatory effects of resveratrol are core to its protective effect, and it may be a good potential therapeutic agent enhancing reproductive health and pregnancy outcomes in animals under heat stress. Since it has potential of restoring cellular function and enhancing reproductive efficiency, additional studies should be conducted to maximize dosages, routes of delivery, and species-specific use, especially in livestock and other animals of economic value.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108044"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-based nanoconjugated hormone delivery boosts reproductive efficiency in male Labeo rohita","authors":"Mohd Ashraf Malik ,&nbsp;Subodh Gupta ,&nbsp;Tincy Varghese ,&nbsp;Shrinivas Jahageerdar ,&nbsp;Sunil Kumar Nayak ,&nbsp;Dhalongsaih Reang","doi":"10.1016/j.anireprosci.2025.108041","DOIUrl":"10.1016/j.anireprosci.2025.108041","url":null,"abstract":"<div><div>Hormone-induced breeding is pivotal in freshwater aquaculture, yet conventional gonadotropin-releasing hormone analogues (GnRH-a) are limited by their rapid degradation and cause stress associated with high-dose injections. Chitosan nanocarriers offer improved hormone stability and delivery. This study evaluated the reproductive efficacy of chitosan nanoconjugated salmon GnRH-a (ChN-sGnRH-a), alone or with domperidone, in male <em>Labeo rohita</em>. Six treatments were tested: Cn (Negative control, nanocarrier control), Cp (Positive control, commercial GnRH-a: Gonopro-FH® at 0.1 ml/kg), T1 (ChN-sGnRH-a at full dose, 0.1 ml/kg), T2 (T1 + domperidone 10 mg/ml), T3 (ChN-sGnRH-a at half dose, 0.05 ml/kg), and T4 (T3 + domperidone 10 mg/ml). T2 resulted in significantly higher (P &lt; 0.05) relative milt volume (8.11 ± 0.47 ml/kg), sperm motility (85.7 ± 1.8 %), and sperm count (3.97 ± 0.05 × 10 ¹⁰/ml) compared to Cp. Serum testosterone (5.2 ± 0.3 ng/ml), 11-ketotestosterone (4.8 ± 0.2 ng/ml), and 17α,20β-dihydroxyprogesterone (2.1 ± 0.1 ng/ml) peaked at 12 h post-injection in T2, sustaining elevated levels over 18 h. T2 also showed significantly (P &lt; 0.05) higher expression of <em>fshβ, lhβ, fshr,</em> and <em>lhr</em> genes<em>,</em> along with advanced testicular histoarchitecture marked by abundant spermatozoa and active leydig/sertoli cells. Principal component analysis revealed that T2, T4 and Cp grouped separately with more than 97 % variance covered by reproductive indices. Notably, T4 (half-dose) was comparable to Cp, suggesting efficacy at reduced hormone dosage. Overall, the findings demonstrate that full-dose ChN-sGnRH-a coadministered with domperidone is superior to commercial GnRH-a, and half-dose nanoconjugates provide equal efficacy, justifying a dose-reduction regime for sustainable, low-stress broodstock management of <em>L. rohita</em>.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108041"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of all-male large yellow croaker (Larimichthys crocea) and evaluation of its temperature tolerance and hypoxia tolerance","authors":"Yang Liu ,&nbsp;Jie Ding ,&nbsp;Tianyi Lan ,&nbsp;Li Wang ,&nbsp;Weiliang Shen ,&nbsp;Xiongfei Wu ,&nbsp;Daojun Tang ,&nbsp;Junquan Zhu","doi":"10.1016/j.anireprosci.2025.108042","DOIUrl":"10.1016/j.anireprosci.2025.108042","url":null,"abstract":"<div><div>Mono-sex cultures of all-male large yellow croaker (<em>Larimichthys crocea</em>) offers clear advantages for boosting economic returns in aquaculture. The purpose of this study was to breed all-male large yellow croaker. To achieve this goal, we first successfully induced sex reversal of YY supermale fish to produce YY pseudo-female through adding 17β-estradiol (E₂, 120 mg/kg). These YY pseudo-female were then crossed with YY supermale fish to establish a pure-bred YY supermale fish population. Subsequently, the YY supermale fish were hybridized with female fish to produce all-male fish. During the breeding process, YY pseudo-female, YY supermale and XY all-male fish were identified by sex-specific molecular markers and gonad histological observation. The results showed that in the experiment of inducing sex reversal of YY supermale fish. The survival rate of YY pseudo-female fish was 63.4 %, and the hatching rate of its offspring pure-bred YY supermale fish was 68.06 %. There was no significant difference in growth, testes development stage, sex steroid hormone levels and the expression of testis development-related genes levels between pure-bred YY supermale and normal male fish. In the temperature tolerance assay, the all-male group exhibited significantly higher survival rates than the control under both high- and low-temperature stress. In the hypoxia tolerance experiment, the respiratory oxygen consumption rate of all-male fish decreased more with the decrease of dissolved oxygen in water, and the asphyxiation point was lower. It shows that the all-male fish is more adaptable to the adverse environment. In summary, it is feasible to obtain a pure-bred YY supermale fish population by inducing YY supermale fish sex reversal. On this basis, the all-male fish population can be produced, and the stress resistance advantages of all-male fish have considerable application potential for the aquaculture of large yellow croakers.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"283 ","pages":"Article 108042"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145556141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}