Ankara Universitesi Veteriner Fakultesi Dergisi最新文献

{"title":"Safkan Bir Atın Lokal Polyester Reçine ile Kesitli Böbrek Plastinasyonunun Anatomik Değerlendirmesi ve Hazırlama Prosedürü","authors":"Caner Bakici, Hasen Awel Yunus, Barış Batur, O. Ekim, Selçuk Tunali","doi":"10.33988/auvfd.1104488","DOIUrl":"https://doi.org/10.33988/auvfd.1104488","url":null,"abstract":"Bu çalışma, lokal polyester reçine ile böbreğin ince kesit plastinasyonu için bir protokol geliştirmeyi ve örneğin anatomik detaylarını incelemeyi amaçlamıştır. Örnek %10 formalin fiksasyon solüsyonu ile sabitlendi ve ardından 3 mm'lik dilimlere ayrıldı. Polyester plastinasyon tekniğinin dört aşaması kullanıldı. İlk olarak örnekler dehidrasyon için -25ºC'de %99,5 aseton banyosunda tutulmuştur. Bu işlemden sonra kesitler polyester reçineye yerleştirildi ve vakum altında oda sıcaklığında (20ºC) impregnasyon işlemine başlandı. Zorlu impregnasyon işleminden sonra kürleme odaları oluşturuldu ve kürleme işlemine ultraviyole ışık altında devam edildi. Uygulanan her aşamanın verileri dikkatlice kaydedildi ve böbrek bölümlerinin polyester plastinasyonu için protokol başarıyla oluşturuldu. Örnekler, ince bir kesit görünümü sayesinde farklı bir bakış açısıyla gözlemlenmiştir. Örneklerin anatomik morfolojisi ve böbreğin kesit bölümlerine ait yapılar, böbrek paraşimi ve dolaşım bileşenleri gibi, iyi korunmuştur. Elde edilen son ürünler, böbreklerin kesitsel anatomik çalışmaları için iyi birer eğitim materyalleri olarak kullanılabileceği düşünülmektedir.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69596070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Escherichia coli O157 and Listeria monocytogenes presence and antibiotic susceptibility isolated from ruminant feces and feeds in Balikesir province","authors":"O. Babacan","doi":"10.33988/auvfd.1007572","DOIUrl":"https://doi.org/10.33988/auvfd.1007572","url":null,"abstract":"In this study, which was conducted for the first time in Balıkesir, it was purposed to determine the existense, virulence factors and antibiotic susceptibility of Escherichia coli O157, which is zoonotic in ruminant feces and feed, and the pathogen Listeria monocytogenes, which causes diseases in humans and animals, and to use these results as epidemiological data in our province, region and country. Feces and animal feed samples were analyzed simultaneously for in order of E. coli O157 and L. Monocytogenes according to ISO 16654: 2001 / Amd 1: 2017 and ISO 11290-1. 38 E.coli O157 was isolated from a total of one hunderd stool samples. 18 L. monocytogenes were isolated from a total of one hunderd stool samples. 6 L. monocytogenes were isolated from 50 silage samples. Three of these isolates were isolated from faeces and silage samples taken from the same farm with L. monocytogenes isolates isolated from sheep feces. E. coli O157 could not be isolated from a total of 100 silage and feed samples. All L. monocytogenes isolates were susceptible to sulfamethoxazole / trimethoprim, tetracycline, streptomycin, meropenem and erythromycin. The highest resistance was detected against Sulbactam / ampicillin. 3 E. coli O157 isolates were found resistant to Gentamicin and 7 isolates to Tobramycin. 21 isolates were resistant to erythromycin, and 12 isolates were intermediate. According to PCR results of fliCH7, Stx1, Stx2, eaeA and EhlyA genes, EhlyA gene was found in 20 E. coli O157 isolates. Of these isolates, 4 were isolated from sheep feces and 16 from calf feces. The stx1 gene was found in a total of 5 E. coli O157 isolates, one from sheep feces and four from calf feces. EhlyA gene was also found in all isolates with stx1 gene. The stx2 gene was found in a total of 3 E. coli O157 isolates, one from sheep dung and two from calf dung. Intimin gene was found in 8 E. coli O157 isolates, two of which are sheep faeces and six calf faecal isolates. EhlyA gene was found in all isolates with intimin gene. In this study, enterohaemolysin is the predominant virulence factor among the isolates. Epidemiologically, silage was thought to be the main source of L. monocytogenes contamination, and recently, silage contamination continued in Balikesir.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"35 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69592150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of PCR for detection of Burkholderia mallei in Turkey","authors":"S. Ekici, O. Dudakli, D. Dülger, Maksut Murat Maden, Ayşe Demi̇rhan","doi":"10.33988/auvfd.1049887","DOIUrl":"https://doi.org/10.33988/auvfd.1049887","url":null,"abstract":"Within the scope of the \"National Ruam Eradication Project\" carried out between 2000-2001, was eradicated Glanders in our country. Unfortunately, 81 horses were culled in Turkey in December 2019, following the detection of Epidemic in horses in Büyükada. In 2019, Glanders were reported in horses in Uşak and Bolu. No human cases have been reported. Global warming, illegal animal and human movements, regional civil wars, poverty, and e-environmental changes are predisposing factors for the emergence and worldwide spread of zoonotic infections such as glanders. Turkey is at risk for Glanders because of its geography. Therefore, reliable detection of Burkholderia mallei by PCR in a short time will prevent the distribution of unwanted infections that may occur in the future throughout the country. In this study; Burkholderia mallei strains isolated from horses were verified and optimized by PCR. The use of PCR for the detection of Burkholderia mallei was performed for the first time in our country. It has been concluded that the PCR method can be safely used in laboratory analyzes together with serology as a diagnostic method with high reliability and sensitivity for both diagnoses.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69594893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The quantitative evaluation of cardiac structures and major thoracic vessel dimensions by means of lateral contrast radiography in healthy, male Wistar albino rats (Rattus norvegicus)","authors":"M. Kaya, M. A. Çeti̇nkaya, Doğa Besne","doi":"10.33988/auvfd.895668","DOIUrl":"https://doi.org/10.33988/auvfd.895668","url":null,"abstract":"The aim of this study was to define reference values for vertebral heart score (VHS) and modified left atrium (LA)-VHS, cardiac structures, and major thoracic vessels measurements and ratios obtained from thoracic contrast radiography in Wistar albino rats. VHS, modified LA-VHS, left (LV) and right (RV) ventricles, interventricular septum (IVS), aorta (Ao), caudal vena cava (CaVC), and fourth thoracic vertebrae (v) length (T4) were measured from left lateral thoracic contrast radiographs of 50 young, healthy adult male Wistar albino rats. LV/T4, RV/T4, IVS/T4, Ao/T4, CaVC/T4, and CaVC/ Ao ratios were calculated from these values. Measurements were performed by two observers unaware of the signalment data. Median values and references ranges were 8.2v (7.4-9.1) for VHS, 1.2 (1.0-1.5) for modified LA-VHS, 7.8 mm (6.2-9.1) for LV, 3.4 mm (2.8-4.5) for RV, 2.1 mm (1.8-3.0) for IVS, 2.1 mm (1.8-2.8) for Ao, 2.2 mm (1.7-3.0) for CaVC, 4 mm (3.5-4.5) for T4, 2 (1.5-2.3) for LV/T4, 0.85 (0.68-1.22) for RV/T4, 0.52 (0.42-0.83) for IVS/T4, 0.53 (0.42-0.75) for Ao/T4, 0.55 (0.45-0.7) for CaVC/T4 and 1.05 (0.74-1.1.37) for CaVC/Ao. Further studies are now needed to determine whether measurement values obtained from contrast radiography in rats are useful in the diagnosis of cardiomyopathy and heart failure. The radiographic measurement values presented in this study can be used as a reference baseline for both pet and laboratory rats.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69604297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Short-Term Transportation and Lairage Time on Meat Quality, CAST, CAPN1, CAPN3, HSPA1A Gene Expression Levels and Myofibrillary Structure of M. longissimus dorsi of Kıvırcık Breed Sheep","authors":"N. Bilgen, A. Ceylan, Funda Yilmaz Eker, E. Akkaya, İsmail Harem, B. Çınar Kul, Enver Barış Bi̇ngöl, F. Esen Gürsel, A. Ateş, D. Özen, Ö. Çeti̇n, A. Bayraktaroglu, T. Kahraman","doi":"10.33988/auvfd.1061335","DOIUrl":"https://doi.org/10.33988/auvfd.1061335","url":null,"abstract":"The objective of this study was to examine the effect of short term transportation and lairage time on meat quality, myofibrillary structure and calpastatin (CAST), µ-calpain (CAPN1), calpain 3 (CAPN3) and heat shock protein 70 (HSPA1A) expression levels of longissimus dorsi of Kıvırcık breed sheep (n=40). Meat quality was evaluated by muscle glycogen amounts, pH, temperature, cooking loss, water holding capacity, shear force, instrumental color, sarcomere length and organoleptic properties. Cellular changes in the muscle during the ageing process was scanned by electron microscopy and CAST, CAPN1, CAPN3, HSPA1A expression levels were measured to reveal the association on meat tenderness. As a result, positive effect of long lairage time groups was observed in the pH, glycogen, SF values and organoleptic evaluation. CAST, CAPN1, CAPN3 expression showed no difference, however, HSPA1A showed significant difference in aging process. In conclusion, genotypic differences, their effect on gene expression and protein level on meat quality should be further investigated.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69595195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Biofilms in Veterinary Medicine","authors":"F. Kiran, Başar Karaca, A. Erdoğan","doi":"10.33988/auvfd.1097786","DOIUrl":"https://doi.org/10.33988/auvfd.1097786","url":null,"abstract":"Microbial biofilms defined as extremely complex ecosystems are considered clinically important for humans. However, the concept and significant roles of microbial biofilms in the progression of disease has seriously lagged in veterinary medicine, when compared with human medicine. Although the research on biofilms in animals is just beginning to emerge, limited studies have paid attention that microbial biofilms are clinically important in the field of veterinary medicine, especially the livestock industry, and lead to serious economic losses. In this review, the importance of microbial biofilms causing high economic losses in the livestock industry has been highlighted. Besides, the concept of microbial biofilm, their role in the pathogenesis of the animal disease, as well as diagnosis approaches and possible therapeutic strategies needed to overcome their detrimental effects in veterinary medicine, has been discussed.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48868121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Protective Effect of Caffeic acid Phenethyl Ester on Cadmiyum-induced Liver Toxicity: A Histopathological and Biochemical Study","authors":"H. Deveci, G. Nur, A. Kükürt, M. Kuru, A. Deveci","doi":"10.33988/auvfd.1068527","DOIUrl":"https://doi.org/10.33988/auvfd.1068527","url":null,"abstract":"In this study, the changes caused by caffeic acid phenethyl ester (CAPE) in the histopathological and biochemical parameters in the oxidant / antioxidant balance in mice with experimental cadmium toxicity were investigated. Plasma paraoxonase (PON) activity, high-density lipoprotein (HDL), low density lipoprotein (LDL), total sialic acid (TSA), total antioxidant capacity (TAC), total oxidant capacity (TOC), and oxidative stress index (OSI) were analyzed on mice’s blood samples. The results showed that cadmium intoxication triggered oxidative stress in the mice. It also lowered their PON activity alongside TAC and HDL levels and increased their TSA, LDL, TOC, and OSI levels. The histopathological examination of the liver tissues revealed focal apoptotic regions, sinusoidal occlusion, and irregularity in the cadmium group and no significant change in the other groups. These results indicated that CAPE can significantly prevent biochemical and histopathological changes due to cadmium damage.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69595373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining the variables affecting the prices of animal-source foods by the network analysis method, the case of Turkey","authors":"A. Akin, Murat Polat, B. Mat, M. B. Çevrimli, M. Arikan, Z. Özel, M. Tekindal","doi":"10.33988/auvfd.1065921","DOIUrl":"https://doi.org/10.33988/auvfd.1065921","url":null,"abstract":"Recently, Turkey has seen significant increases in animal-source foods and related raw material prices. These recent increases in prices have made it even more important to examine the prices of animal-source foods and their associated factors. This study aimed to reveal the pattern network structure and characteristics of the prices of animal-source foods and related variables for the 2010-2020 period in Turkey. To this end, a network analysis covered the prices of animal-source foods, raw material prices used in producing these foods, and some economic variables. The study results revealed that Producer Price Index (PPI) and Agricultural PPI are key variables that are highly active in the network and act as a bridge between other unconnected variables. In addition, the results of the analysis suggest that the overall network consists of highly correlated variables and that the PPI and Agricultural PPI are the two most important variables. As a result, the pattern network structure shows that economic variables have a high impact on the prices of animal-source foods. The network structure visualizes Turkey's import-substitution model in animal production and foreign dependency on feed raw materials and the market structure affected by high exchange rates. In addition, as can be understood from the positive connections between feed raw materials, the pattern network of the variables affecting the prices of animal-source foods is largely shaped by feed prices and the internal dynamics of economic variables.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69595330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular detection of cystic echinococcosis diagnosed incidentally in exploratory laparotomy of a domestic cat (Felis catus) in Hatay, Turkey","authors":"İ. Erdem, Aykut Zerek, Sibel ELMACIOĞLU CURA, M. Yaman, M. Deveci, Ömer Kırgız","doi":"10.33988/auvfd.1062916","DOIUrl":"https://doi.org/10.33988/auvfd.1062916","url":null,"abstract":"In this case report, in a twelve years old domestic cat is reported cystic echinococcosis which detected during exploratory laparotomy. In the anamnesis, there was a complaint of progressive abdominal swelling. As a result of clinical and radiological examinations, was detected unknown intraabdominal formations. At laparotomy were found to be multiple cysts on the right and left kidneys. As a result of molecular analysis, hydatid cyst, which is the larva of Echinococcus granulosus, was detected. This study is the first case of cystic echinococcosis detected molecularly in a domestic cat in Turkey.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69594828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of physical forms of corn and forage source on growth performance, blood parameters and ruminal fermentation of Holstein calves","authors":"Safura Jabbari, J. Seifdavati, G. Ghorbani, Hosein Abdi-Benemar, R. Seyed Sharifi, Sayyad Seifzadeh","doi":"10.33988/auvfd.1025207","DOIUrl":"https://doi.org/10.33988/auvfd.1025207","url":null,"abstract":"This investigation's purpose was the evaluation of the effects of physical forms of corn and forage sources on growth performance, blood parameters, and ruminal fermentation of Holstein calves. Forty-eight Holstein calves (38.2 ± 1.2 kg of BW) were used in a completely randomized design with 4 treatments and 12 replications for 65 days. The treatments were: 1) Basal diet + mashed form corn + wheat straw (MCWS), 2) Basal diet + flacked form corn + wheat straw (FCWS), 3) Basal diet + mashed form corn+ Sugarcane bagasse (MCSB) and 4) Basal diet + flacked form corn + sugarcane bagasse (FCSB). Final body weight and daily weight gain were affected significantly by the forage (F) factor whereas no effect from C (corn physical forms) and C×F interaction were observed. Skeletal growth parameters were not affected by C, F, and F×C factors. The trial factors did not affect triglyceride and BHBA levels, while glucose level and urea were affected by C and F factors. At 30 and 60 days, Calves fed on FCWS had the highest glucose. Also, calves fed the FCSB diet presented higher urea at 30 and 60 days. Acetate acid was affected significantly by C, F, and C×F interaction (P","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69592012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}