Use of PCR for detection of Burkholderia mallei in Turkey

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Ankara Universitesi Veteriner Fakultesi Dergisi Pub Date : 2022-06-17 DOI:10.33988/auvfd.1049887

S. Ekici, O. Dudakli, D. Dülger, Maksut Murat Maden, Ayşe Demi̇rhan

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引用次数: 0

Abstract

Within the scope of the "National Ruam Eradication Project" carried out between 2000-2001, was eradicated Glanders in our country. Unfortunately, 81 horses were culled in Turkey in December 2019, following the detection of Epidemic in horses in Büyükada. In 2019, Glanders were reported in horses in Uşak and Bolu. No human cases have been reported. Global warming, illegal animal and human movements, regional civil wars, poverty, and e-environmental changes are predisposing factors for the emergence and worldwide spread of zoonotic infections such as glanders. Turkey is at risk for Glanders because of its geography. Therefore, reliable detection of Burkholderia mallei by PCR in a short time will prevent the distribution of unwanted infections that may occur in the future throughout the country. In this study; Burkholderia mallei strains isolated from horses were verified and optimized by PCR. The use of PCR for the detection of Burkholderia mallei was performed for the first time in our country. It has been concluded that the PCR method can be safely used in laboratory analyzes together with serology as a diagnostic method with high reliability and sensitivity for both diagnoses.

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PCR检测土耳其马尔氏伯克氏菌的方法

在2000-2001年实施的“国家根除疟疾工程”的范围内，我国消灭了白喉。不幸的是，2019年12月，在贝尔 ykada的马匹中发现流行病后，土耳其扑杀了81匹马。2019年，据报道在u ak和Bolu的马匹中发现了腺疣。尚未报告人间病例。全球变暖、动物和人类的非法流动、地区内战、贫困和电子环境变化是人畜共患传染病(如腺病)出现和在世界范围内传播的诱发因素。由于土耳其的地理位置，格兰德斯面临风险。因此，通过PCR在短时间内可靠地检测出马氏伯克霍尔德氏菌，将防止未来可能在全国范围内发生的不必要的感染分布。在本研究中;对马源马氏伯克霍尔德菌进行了PCR验证和优化。采用PCR法检测马尔氏伯克霍尔德菌在我国尚属首次。结果表明，PCR与血清学作为一种可靠、灵敏的诊断方法，可安全用于实验室分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ankara Universitesi Veteriner Fakultesi Dergisi 农林科学-兽医学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ankara Üniversitesi Veteriner Fakültesi Dergisi is one of the journals’ of Ankara University, which is the first well-established university in the Republic of Turkey. Research articles, short communications, case reports, letter to editor and invited review articles are published on all aspects of veterinary medicine and animal science. The journal is published on a quarterly since 1954 and indexing in Science Citation Index-Expanded (SCI-Exp) since April 2007.