巴利克西尔省反刍动物粪便和饲料中O157大肠杆菌和单核增生李斯特菌的分离及药敏调查

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Ankara Universitesi Veteriner Fakultesi Dergisi Pub Date : 2022-06-27 DOI:10.33988/auvfd.1007572

O. Babacan

{"title":"巴利克西尔省反刍动物粪便和饲料中O157大肠杆菌和单核增生李斯特菌的分离及药敏调查","authors":"O. Babacan","doi":"10.33988/auvfd.1007572","DOIUrl":null,"url":null,"abstract":"In this study, which was conducted for the first time in Balıkesir, it was purposed to determine the existense, virulence factors and antibiotic susceptibility of Escherichia coli O157, which is zoonotic in ruminant feces and feed, and the pathogen Listeria monocytogenes, which causes diseases in humans and animals, and to use these results as epidemiological data in our province, region and country. Feces and animal feed samples were analyzed simultaneously for in order of E. coli O157 and L. Monocytogenes according to ISO 16654: 2001 / Amd 1: 2017 and ISO 11290-1. 38 E.coli O157 was isolated from a total of one hunderd stool samples. 18 L. monocytogenes were isolated from a total of one hunderd stool samples. 6 L. monocytogenes were isolated from 50 silage samples. Three of these isolates were isolated from faeces and silage samples taken from the same farm with L. monocytogenes isolates isolated from sheep feces. E. coli O157 could not be isolated from a total of 100 silage and feed samples. All L. monocytogenes isolates were susceptible to sulfamethoxazole / trimethoprim, tetracycline, streptomycin, meropenem and erythromycin. The highest resistance was detected against Sulbactam / ampicillin. 3 E. coli O157 isolates were found resistant to Gentamicin and 7 isolates to Tobramycin. 21 isolates were resistant to erythromycin, and 12 isolates were intermediate. According to PCR results of fliCH7, Stx1, Stx2, eaeA and EhlyA genes, EhlyA gene was found in 20 E. coli O157 isolates. Of these isolates, 4 were isolated from sheep feces and 16 from calf feces. The stx1 gene was found in a total of 5 E. coli O157 isolates, one from sheep feces and four from calf feces. EhlyA gene was also found in all isolates with stx1 gene. The stx2 gene was found in a total of 3 E. coli O157 isolates, one from sheep dung and two from calf dung. Intimin gene was found in 8 E. coli O157 isolates, two of which are sheep faeces and six calf faecal isolates. EhlyA gene was found in all isolates with intimin gene. In this study, enterohaemolysin is the predominant virulence factor among the isolates. Epidemiologically, silage was thought to be the main source of L. monocytogenes contamination, and recently, silage contamination continued in Balikesir.","PeriodicalId":7874,"journal":{"name":"Ankara Universitesi Veteriner Fakultesi Dergisi","volume":"35 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of Escherichia coli O157 and Listeria monocytogenes presence and antibiotic susceptibility isolated from ruminant feces and feeds in Balikesir province\",\"authors\":\"O. Babacan\",\"doi\":\"10.33988/auvfd.1007572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, which was conducted for the first time in Balıkesir, it was purposed to determine the existense, virulence factors and antibiotic susceptibility of Escherichia coli O157, which is zoonotic in ruminant feces and feed, and the pathogen Listeria monocytogenes, which causes diseases in humans and animals, and to use these results as epidemiological data in our province, region and country. Feces and animal feed samples were analyzed simultaneously for in order of E. coli O157 and L. Monocytogenes according to ISO 16654: 2001 / Amd 1: 2017 and ISO 11290-1. 38 E.coli O157 was isolated from a total of one hunderd stool samples. 18 L. monocytogenes were isolated from a total of one hunderd stool samples. 6 L. monocytogenes were isolated from 50 silage samples. Three of these isolates were isolated from faeces and silage samples taken from the same farm with L. monocytogenes isolates isolated from sheep feces. E. coli O157 could not be isolated from a total of 100 silage and feed samples. All L. monocytogenes isolates were susceptible to sulfamethoxazole / trimethoprim, tetracycline, streptomycin, meropenem and erythromycin. The highest resistance was detected against Sulbactam / ampicillin. 3 E. coli O157 isolates were found resistant to Gentamicin and 7 isolates to Tobramycin. 21 isolates were resistant to erythromycin, and 12 isolates were intermediate. According to PCR results of fliCH7, Stx1, Stx2, eaeA and EhlyA genes, EhlyA gene was found in 20 E. coli O157 isolates. Of these isolates, 4 were isolated from sheep feces and 16 from calf feces. The stx1 gene was found in a total of 5 E. coli O157 isolates, one from sheep feces and four from calf feces. EhlyA gene was also found in all isolates with stx1 gene. The stx2 gene was found in a total of 3 E. coli O157 isolates, one from sheep dung and two from calf dung. Intimin gene was found in 8 E. coli O157 isolates, two of which are sheep faeces and six calf faecal isolates. EhlyA gene was found in all isolates with intimin gene. In this study, enterohaemolysin is the predominant virulence factor among the isolates. Epidemiologically, silage was thought to be the main source of L. monocytogenes contamination, and recently, silage contamination continued in Balikesir.\",\"PeriodicalId\":7874,\"journal\":{\"name\":\"Ankara Universitesi Veteriner Fakultesi Dergisi\",\"volume\":\"35 1\",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2022-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ankara Universitesi Veteriner Fakultesi Dergisi\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.33988/auvfd.1007572\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ankara Universitesi Veteriner Fakultesi Dergisi","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.33988/auvfd.1007572","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

本研究首次在Balıkesir开展，目的是确定反刍动物粪便和饲料中人畜共患的大肠杆菌O157和人畜致病的单核增生李斯特菌的存在情况、毒力因素及抗生素敏感性，并将这些结果作为我省、地区和全国流行病学资料。按照ISO 16654: 2001 / Amd 1:20 17和ISO 11290-1的要求，同时对粪便和饲料样品进行大肠杆菌O157和单核增生乳杆菌的检测。共从100份粪便样本中分离出38株大肠杆菌O157。从100份粪便样本中分离到18株单核细胞增生乳杆菌。从50份青贮样品中分离到6株单核增生乳杆菌。其中3个分离株是从同一农场的粪便和青贮样品中分离出来的，另外3个分离株是从绵羊粪便中分离出来的。从100份青贮料和饲料样品中均无法分离到大肠杆菌O157。所有单核增生乳杆菌对磺胺甲恶唑/甲氧苄啶、四环素、链霉素、美罗培南和红霉素敏感。对舒巴坦/氨苄西林的耐药性最高。大肠杆菌O157对庆大霉素耐药3株，妥布霉素耐药7株，红霉素耐药21株，中间耐药12株。根据fliCH7、Stx1、Stx2、eaeA和EhlyA基因的PCR结果，在20株大肠杆菌O157中检出EhlyA基因。其中4株分离自绵羊粪便，16株分离自小牛粪便。共在5株大肠杆菌O157分离株中发现stx1基因，其中1株来自羊粪，4株来自小牛粪便。所有stx1基因分离株中均存在EhlyA基因。在3株大肠杆菌O157分离株中发现了stx2基因，1株来自羊粪，2株来自小牛粪。8株大肠杆菌O157分离株中检出内膜素基因，其中2株为绵羊粪便，6株为小牛粪便。所有带内膜素基因的分离株均检出EhlyA基因。在本研究中，肠溶血素是主要的毒力因子。在流行病学上，青贮饲料被认为是单核增生乳杆菌污染的主要来源，近年来，青贮饲料污染在巴利克西尔地区持续存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Investigation of Escherichia coli O157 and Listeria monocytogenes presence and antibiotic susceptibility isolated from ruminant feces and feeds in Balikesir province

In this study, which was conducted for the first time in Balıkesir, it was purposed to determine the existense, virulence factors and antibiotic susceptibility of Escherichia coli O157, which is zoonotic in ruminant feces and feed, and the pathogen Listeria monocytogenes, which causes diseases in humans and animals, and to use these results as epidemiological data in our province, region and country. Feces and animal feed samples were analyzed simultaneously for in order of E. coli O157 and L. Monocytogenes according to ISO 16654: 2001 / Amd 1: 2017 and ISO 11290-1. 38 E.coli O157 was isolated from a total of one hunderd stool samples. 18 L. monocytogenes were isolated from a total of one hunderd stool samples. 6 L. monocytogenes were isolated from 50 silage samples. Three of these isolates were isolated from faeces and silage samples taken from the same farm with L. monocytogenes isolates isolated from sheep feces. E. coli O157 could not be isolated from a total of 100 silage and feed samples. All L. monocytogenes isolates were susceptible to sulfamethoxazole / trimethoprim, tetracycline, streptomycin, meropenem and erythromycin. The highest resistance was detected against Sulbactam / ampicillin. 3 E. coli O157 isolates were found resistant to Gentamicin and 7 isolates to Tobramycin. 21 isolates were resistant to erythromycin, and 12 isolates were intermediate. According to PCR results of fliCH7, Stx1, Stx2, eaeA and EhlyA genes, EhlyA gene was found in 20 E. coli O157 isolates. Of these isolates, 4 were isolated from sheep feces and 16 from calf feces. The stx1 gene was found in a total of 5 E. coli O157 isolates, one from sheep feces and four from calf feces. EhlyA gene was also found in all isolates with stx1 gene. The stx2 gene was found in a total of 3 E. coli O157 isolates, one from sheep dung and two from calf dung. Intimin gene was found in 8 E. coli O157 isolates, two of which are sheep faeces and six calf faecal isolates. EhlyA gene was found in all isolates with intimin gene. In this study, enterohaemolysin is the predominant virulence factor among the isolates. Epidemiologically, silage was thought to be the main source of L. monocytogenes contamination, and recently, silage contamination continued in Balikesir.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Ankara Universitesi Veteriner Fakultesi Dergisi 农林科学-兽医学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ankara Üniversitesi Veteriner Fakültesi Dergisi is one of the journals’ of Ankara University, which is the first well-established university in the Republic of Turkey. Research articles, short communications, case reports, letter to editor and invited review articles are published on all aspects of veterinary medicine and animal science. The journal is published on a quarterly since 1954 and indexing in Science Citation Index-Expanded (SCI-Exp) since April 2007.