Anatomy & Cell Biology最新文献_第2页

The foramen of Magendie: a comprehensive review of its history, anatomy, pathology, and surgical approaches. Magendie孔：其历史，解剖，病理和手术入路的全面回顾。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-05-20 DOI: 10.5115/acb.26.009

Brendan Bych, Brianna L Hines, Noritaka Komune, Joseph Lockwood, Carmine Antonio Donofrio, Filippo Badaloni, Antonio Fioravanti, Aaron S Dumont, Joe Iwanaga, R Shane Tubbs

{"title":"The foramen of Magendie: a comprehensive review of its history, anatomy, pathology, and surgical approaches.","authors":"Brendan Bych, Brianna L Hines, Noritaka Komune, Joseph Lockwood, Carmine Antonio Donofrio, Filippo Badaloni, Antonio Fioravanti, Aaron S Dumont, Joe Iwanaga, R Shane Tubbs","doi":"10.5115/acb.26.009","DOIUrl":"10.5115/acb.26.009","url":null,"abstract":"The foramen of Magendie (FOM), or median aperture, represents a critical anatomical structure allowing cerebrospinal fluid outflow from the fourth ventricle into the cisterna magna. Since its description by François Magendie in the 19th century, its existence, embryological development, and clinical importance have been subjects of debate and refinement. This review synthesizes the historical context, developmental biology, and anatomical nuances of the FOM, alongside its involvement in congenital and acquired pathologies, particularly Blake's pouch cysts, Dandy-Walker malformations, and obstructive neoplasms. We further analyze controversies surrounding its recognition in early literature, anatomical variants including agenesis, and its surgical implications. Surgical approaches such as the telovelar and transvermian routes are compared, with emphasis on minimizing morbidity while ensuring exposure. Advances in neuroimaging and microsurgical technique have reinforced the FOM's relevance in modern neurosurgery. A comprehensive understanding of its anatomy, pathology, and historical discourse is essential for anatomists, neurosurgeons, and clinicians managing posterior fossa pathologies.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"368-374"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer activity of Hebesu extracts in cancer cell lines. 鹤besu提取物对癌细胞的抗癌作用。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-05-15 DOI: 10.5115/acb.26.031

Eun-Jung Kim, Sohyun Kim, Nayeon Bak, Senthil Kumar Baskaran, Suyeon Lee, Junsu Kim, Masahito Uchiyama, Jong-Min Lee, Han-Sung Jung

{"title":"Anticancer activity of Hebesu extracts in cancer cell lines.","authors":"Eun-Jung Kim, Sohyun Kim, Nayeon Bak, Senthil Kumar Baskaran, Suyeon Lee, Junsu Kim, Masahito Uchiyama, Jong-Min Lee, Han-Sung Jung","doi":"10.5115/acb.26.031","DOIUrl":"10.5115/acb.26.031","url":null,"abstract":"Hebesu, a small citrus fruit from Miyazaki, Japan, has the potential to advance anticancer activity due to its high content of bioactive flavonoids, including narirutin, naringin, hesperidin, and neohesperidin. This study explored the anticancer effects of Hebesu extract (HBS) in various human cancer cell lines: MDA-MB-231 (breast cancer), HepG2 (liver cancer), and A549 (lung cancer). The results showed that treatment with HBS led to a substantial reduction in cell viability in a dose-dependent manner and induced morphological changes, accompanied by increased expression of apoptosis-related markers. TdTomato fluorescence imaging demonstrated that cytotoxicity in breast cancer cells was dependent on both the concentration and duration of exposure. Furthermore, HBS reduced Ki-67 expression, increased the levels of Caspase-3 and NRF2, and downregulated the expression of the oncogenic genes SMO, KRAS, and BRAF. These findings suggest that HBS has a multi-targeted anticancer effect by inhibiting cell proliferation, promoting apoptosis, and activating oxidative stress responses, while also suppressing oncogenic signaling pathways. This study emphasizes the potential use of HBS and its flavonoid components as natural cancer suppressive agents. Further investigation using in vivo models and clinical studies are essential to fully assess their efficacy, bioavailability, and safety.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"519-525"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147939771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation potential of human amniotic fluid derived-mesenchymal stem cells into dopaminergic neurons. 人羊水间充质干细胞向多巴胺能神经元分化的潜力。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-07-13 DOI: 10.5115/acb.25.361

Napatsorn Thongdi, Kantirat Yaja, Napatsaree Nuttapasit, Peraphan Pothacharoen, Sirinda Aungsuchawan, Suteera Narakornsak, Waleephan Treebupachatsakul

{"title":"Differentiation potential of human amniotic fluid derived-mesenchymal stem cells into dopaminergic neurons.","authors":"Napatsorn Thongdi, Kantirat Yaja, Napatsaree Nuttapasit, Peraphan Pothacharoen, Sirinda Aungsuchawan, Suteera Narakornsak, Waleephan Treebupachatsakul","doi":"10.5115/acb.25.361","DOIUrl":"10.5115/acb.25.361","url":null,"abstract":"Stem cell-based therapies have emerged as promising alternatives for disease modification and cell replacement, aiming to improve the quality of life in patients with Parkinson's disease. Human amniotic fluid mesenchymal stem cells (hAF-MSCs) represent a viable source due to their self-renewal ability, high proliferative capacity, and multipotent differentiation potential. This study aimed to evaluate the in vitro differentiation potential of hAF-MSCs into dopaminergic neurons. Flow cytometry and Alamar blue assays were employed to confirm mesenchymal stem cell characteristics. The hAF-derived cells fulfilled the established MSCs criteria, including fibroblast-like morphology, surface marker expression, and proliferative capacity. Upon induction with dopaminergic differentiation factors, the cells acquired neuron-like morphology. RT-qPCR analysis revealed significantly upregulated expression of neuronal and dopaminergic-specific genes (nestin, β-III-tubulin, and tyrosine hydroxylase), further supported by immunofluorescent staining showing the presence of neuron-related proteins. These findings demonstrate the potential of hAF-MSCs to differentiate into dopaminergic neurons and highlight their promise for future applications in regenerative medicine.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"438-448"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aripiprazole‑induced alterations in axial skeletal ossification following in utero exposure in rats. 阿立哌唑诱导大鼠子宫内暴露后轴向骨骨化的改变。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-07-01 DOI: 10.5115/acb.26.055

Wael Amin Nasr El-Din, Mariwan Husni, Abdelhalim Salem Deifalla, Bhagath Kumar Potu, Ayesha Fatima, Aisha Rashid, Amer Almarabheh, Raouf Abdelrahman Fadel

{"title":"Aripiprazole‑induced alterations in axial skeletal ossification following in utero exposure in rats.","authors":"Wael Amin Nasr El-Din, Mariwan Husni, Abdelhalim Salem Deifalla, Bhagath Kumar Potu, Ayesha Fatima, Aisha Rashid, Amer Almarabheh, Raouf Abdelrahman Fadel","doi":"10.5115/acb.26.055","DOIUrl":"10.5115/acb.26.055","url":null,"abstract":"Aripiprazole, a third-generation atypical antipsychotic drug, crosses the placental barrier, yet its developmental skeletal effects remain unexplored. This study aimed to evaluate impact of prenatal aripiprazole exposure, administered at three different doses, on the ossification of axial skeleton in 20-day old rat fetuses. Forty pregnant Sprague-Dawley rats were assigned to four groups: control and three aripiprazole treated-groups receiving 3 mg/kg (low dose aripiprazole), 6 mg/kg (high dose aripiprazole), and 12 mg/kg (double high dose aripiprazole [DHDA]) daily from gestational days 6-19. Fetuses were delivered on gestation day 20, weighed, and processed for skeletal evaluation using Alizarin Red staining. Ossification of craniofacial bones, hyoid bone, vertebral centra and arches, sternum, and ribs were assessed and categorized as complete, delayed, or absent. A total of 151 fetuses were analyzed. Aripiprazole exposure induced a dose dependent reduction in fetal weight and in the number of completely ossified skeletal centers. Craniofacial bones, particularly parietal, interparietal, supraoccipital, and presphenoid, were the most affected. Significant impairments were observed in vertebral centra and arches across cervical, thoracic, lumbar, sacral, and coccygeal regions, with the DHDA group exhibiting the greatest deficits. Sternebrae ossification showed marked reduction, whereas rib ossification remained unaffected. A strong positive correlation was found between fetal weight and total number of complete ossification centers across all groups. Prenatal exposure to aripiprazole leads to dose dependent fetal growth restriction and delayed ossification of axial skeleton in rat fetuses. Future investigations should focus on the molecular mechanisms and consequences related to prenatal impacts of aripiprazole.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"457-470"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population-specific reference values for calcaneal angular parameters and their limited utility in sex estimation. 跟骨角度参数的人群特定参考值及其在性别估计中的有限效用。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-07-28 DOI: 10.5115/acb.26.066

Faruk Gazi Ceranoğlu, Mehmet Tuğrul Yılmaz, Duygu Akın Saygın, Cengiz Kadıyoran

{"title":"Population-specific reference values for calcaneal angular parameters and their limited utility in sex estimation.","authors":"Faruk Gazi Ceranoğlu, Mehmet Tuğrul Yılmaz, Duygu Akın Saygın, Cengiz Kadıyoran","doi":"10.5115/acb.26.066","DOIUrl":"10.5115/acb.26.066","url":null,"abstract":"The calcaneus is the largest tarsal bone located in the lower part of the ankle joint and plays a significant role in walking, running, and maintaining balance. Certain angles of this bone, which is of great clinical and anthropological importance, are used in the diagnosis and treatment of fractures. The aim of this study is to determine the mean values of angular parameters of the calcaneus in a healthy population and to evaluate the accuracy rates of these parameters in predicting sex. In this study, six bilateral angle measurements were performed on radiographs from 540 individuals aged 20-65. Measured angles included Böhler, Gissane, calcaneal inclination, Fowler-Philip, lateral talocalcaneal, and calcaneal compression. The obtained data were compared between sexes, age groups, and right-left sides. Additionally, a receiver operating characteristic analysis was performed for sex prediction. Statistical analyses revealed significant differences between right and left sides in the calcaneal inclination angle and lateral talocalcaneal angle, between sexes in the calcaneal compression angle, and between age groups in the Böhler and Gissane angles. Analyses performed for sex prediction showed that the angles were not suitable for this purpose. The findings and the determination of the mean values of the angles provide useful information for decision-making in clinical diagnosis and treatment processes. It was found that using only calcaneal angles is not appropriate for sex prediction in the Turkish population. The findings may contribute to the establishment of population-specific reference values and provide baseline data for future multivariate or machine learning-based investigations.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"402-413"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148597755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute radiation-induced changes in the submandibular salivary gland and possible radioprotective role of silver nanoparticles: a histological, immunohistochemical, and ultrastructural study. 急性辐射引起的颌下腺变化和纳米银可能的辐射防护作用：组织学、免疫组织化学和超微结构研究

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-07-28 DOI: 10.5115/acb.26.073

Asmaa Sayed Shahin, Mohamed Emad El-Din Ibrahim, Doaa Mahmoud Shuaib, Wafaa Mahmoud Abdullah, Abdel Satar Ibrahim

{"title":"Acute radiation-induced changes in the submandibular salivary gland and possible radioprotective role of silver nanoparticles: a histological, immunohistochemical, and ultrastructural study.","authors":"Asmaa Sayed Shahin, Mohamed Emad El-Din Ibrahim, Doaa Mahmoud Shuaib, Wafaa Mahmoud Abdullah, Abdel Satar Ibrahim","doi":"10.5115/acb.26.073","DOIUrl":"10.5115/acb.26.073","url":null,"abstract":"Gamma radiation is widely used in the treatment of head and neck malignancies, it can cause irreversible damage to the submandibular salivary glands, leading to xerostomia and oral infections. This study evaluated the acute radiation-induced changes in the submandibular gland and the prophylactic and therapeutic effects of silver nanoparticles (AgNPs) in adult male albino rats. Forty-five adult male albino rats (150-200 g) were divided into five groups. Twenty rats served as normal and sham controls, while 25 rats received a single 15 Gy dose of gamma radiation and were assigned to irradiated (n=10), prophylactic AgNPs (n=10), and therapeutic AgNPs (n=5) groups. Right submandibular glands were processed for histological and immunohistochemical analyses, and left glands for ultrastructural examination. Irradiated glands showed acinar atrophy, cytoplasmic vacuolation, pyknotic nuclei, ductal dilatation, reduced PAS optical density, and significantly increased CD3 and BAX immunoreactivity compared with controls. Ultrastructural examination revealed clumped chromatin, swollen mitochondria, and disrupted rough endoplasmic reticulum. Prophylactic AgNP administration largely preserved glandular architecture, maintained acinar area and duct diameter, reduced CD3 and BAX expression, and restored normal ultrastructural features. In contrast, therapeutic AgNP administration produced only minimal improvement, with persistent structural abnormalities. Prophylactic AgNP administration effectively attenuated acute gamma radiation-induced submandibular gland injury, whereas post-irradiation treatment provided only limited therapeutic benefit.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"493-514"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148597728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical anatomy of the superior labial branch of infraorbital nerve. 眶下神经唇上支的临床解剖。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-04-27 DOI: 10.5115/acb.25.150

Hiroaki Takakura, Airi Tanai, Yuki Kunisada, Shogo Kikuta, Norio Kitagawa, Soichiro Ibaragi, Mi-Sun Hur, Rizwan Aslam, R Shane Tubbs, Joe Iwanaga

{"title":"Clinical anatomy of the superior labial branch of infraorbital nerve.","authors":"Hiroaki Takakura, Airi Tanai, Yuki Kunisada, Shogo Kikuta, Norio Kitagawa, Soichiro Ibaragi, Mi-Sun Hur, Rizwan Aslam, R Shane Tubbs, Joe Iwanaga","doi":"10.5115/acb.25.150","DOIUrl":"10.5115/acb.25.150","url":null,"abstract":"The infraorbital nerve (ION), a branch of the maxillary division of the trigeminal nerve, provides sensory innervation to the midface via its terminal divisions. Among these, the superior labial branch (SLb) supplies the upper lip and adjacent mucosa, regions frequently involved in oral, maxillofacial, and cosmetic procedures. Despite its clinical importance, the anatomy of the SLb has received relatively limited attention compared with other ION branches. This review synthesizes current evidence on the SLb's course, branching patterns, innervation, morphometry, and variations, with emphasis on its relevance to surgical practice. Anatomical studies demonstrate that the SLb is the largest terminal division of the ION, often exhibiting medial and lateral subdivisions that anastomose with neighboring nerves. Its distribution predominantly follows a vertical orientation, supplying both cutaneous and mucosal structures of the upper lip. Variability in origin, branching, and accessory foramina underscores the need for careful surgical planning. Injury to the SLb is a recognized complication of Le Fort I osteotomy, midfacial trauma, and periapical procedures, potentially leading to long-term sensory disturbances. A comprehensive understanding of the SLb enhances intraoperative nerve preservation and may reduce postoperative morbidity, highlighting its significance for clinicians operating in the midfacial region.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"361-367"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluoride enamel stability and remineralization. 氟牙釉质稳定性和再矿化。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-06-01 DOI: 10.5115/acb.26.046

Junseo Lee, Minsoo Kang, Cal Thomas Wenninger, Jiayun Li, Sophia Hattie Wirtz, Lauren Kim, Dhanvi Kanade, Madeline Lee Dahlk, Rana Essa

{"title":"Fluoride enamel stability and remineralization.","authors":"Junseo Lee, Minsoo Kang, Cal Thomas Wenninger, Jiayun Li, Sophia Hattie Wirtz, Lauren Kim, Dhanvi Kanade, Madeline Lee Dahlk, Rana Essa","doi":"10.5115/acb.26.046","DOIUrl":"10.5115/acb.26.046","url":null,"abstract":"Dental caries remains as one of the most prevalent noncommunicable diseases even with advances in preventive dental care. This literature-based research examines how fluoride exposure influences enamel stability and caries formation. We hypothesized that fluoride reduces caries incidence by promoting remineralization and reinforcing the crystal lattice structure. A comprehensive search was done through PubMed, ScienceDirect, Karger, and Figshare to select peer reviewed studies that evaluated fluoride and enamel interactions. Quantitative data were extracted from open access in vitro and in vivo studies that incorporated transverse microradiography, electron probe microanalysis, micro-computed tomography, and energy-dispersive spectroscopy to fully assess the lesion depth, mineral density, and fluoride's effects on dental tissues. Of the 60 studies that were screened, 23 were met with the inclusion criteria. Across all experimental models, fluoride exposure produced a consistent concentration dependent positive effect in both the enamel and the dentin. In vitro and pH cycling models carious lesion depths approximately decreased 30%-50% relative to the control. Microanalytical measurements demonstrated high fluoride accumulation within the outer enamel surface. The lattice associated fluoride concentrations increased from baseline 200 ppm to over 1,200 ppm in treatment groups. These compositional changes caused improvement in mechanical and chemical stability, where it caused a 15%-25% increase in surface microhardness and a 35%-80% decrease in mineral (Ca2+) loss during acid exposure. Together, the results strongly indicate that fluoride enhances enamel stability through remineralization and lattice stabilizing mechanisms that increase the resistance to acid exposure.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"375-384"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acellular lung matrix scaffold coated by fibronectin enhances hepatic differentiation of adipose-derived mesenchymal stem cells. 纤维连接蛋白包被的脱细胞肺基质支架促进脂肪源性间充质干细胞的肝分化。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-08-04 DOI: 10.5115/acb.26.061

Darioush Bijan Nejad, Seyyed Saeed Azandeh, Vahid Bayati, Mahmoud Orazizadeh, Maryam Baazm, Elham Younesi

{"title":"Acellular lung matrix scaffold coated by fibronectin enhances hepatic differentiation of adipose-derived mesenchymal stem cells.","authors":"Darioush Bijan Nejad, Seyyed Saeed Azandeh, Vahid Bayati, Mahmoud Orazizadeh, Maryam Baazm, Elham Younesi","doi":"10.5115/acb.26.061","DOIUrl":"10.5115/acb.26.061","url":null,"abstract":"End-stage liver disease is a multifactorial disorder and leading cause of death. The development of effective tissue models has been a focus of research for treating liver disease. This study investigated the use of acellular lung matrix (ALM) and fibronectin (FN) to culture adipose-derived mesenchymal stem cells (ADMSCs) and promote their differentiation into hepatic-like cells. In this experimental study, sodium deoxycholate and NaCl were used to decellularize the lungs, and scaffolds were characterized by histology, scanning electron microscopy, and DNA quantification. ADMSCs were differentiated into hepatocyte-like cells using a two-step protocol under three conditions: in two-dimensional culture, on ALM scaffolds, and on FN-coated ALM scaffolds. Hepatic differentiation was confirmed by RT-PCR for specific genes (alpha-fetoprotein [AFP], albumin [ALB], cytokeratin-18 [CK-18]), ALB and urea levels were measured using specific commercial kits. The main extracellular matrix components (collagen and glycosaminoglycans) were preserved, and residual DNA was minimal (P<0.001), indicating effective decellularization. ADMSCs cultured on FN-coated ALM exhibited significantly higher expression of hepatic markers (AFP, P<0.01; ALB, P<0.01; CK-18, P<0.01). Functional assay confirmed higher ALB and urea production (P<0.05), while MTT assay demonstrated that the scaffold was non-cytotoxic. Collectively, these results demonstrate that FN-coated ALM holds considerable potential for facilitating the hepatic differentiation of ADMSCs under the applied induction conditions.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"471-482"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preconditioning of human dental pulp stem cells by H₂O₂ promotes functional recovery in a rat spinal cord contusion injury model. H2O2预处理人牙髓干细胞促进大鼠脊髓挫伤模型功能恢复。

IF 1.1

Anatomy & Cell Biology Pub Date : 2026-09-30 Epub Date: 2026-05-28 DOI: 10.5115/acb.25.066

Maryam Nazm Bojnordi, Saeid Bagheri-Mohammadi, Hatef Ghasemi Hamidabadi

{"title":"Preconditioning of human dental pulp stem cells by H2O2 promotes functional recovery in a rat spinal cord contusion injury model.","authors":"Maryam Nazm Bojnordi, Saeid Bagheri-Mohammadi, Hatef Ghasemi Hamidabadi","doi":"10.5115/acb.25.066","DOIUrl":"10.5115/acb.25.066","url":null,"abstract":"Cell therapy is a potential therapeutic choice to treatment spinal cord injury (SCI). Studies proved that hypoxic precondition has protective effects on damaged cells. It sounds that a sub-lethal hypoxic exposure is likely enhance survival rates of transplanted cells. The aim of this study is the effect a novel strategy of preconditioning of human dental pulp stem cells (hDPSCs) via hydrogen peroxide (H2O2) on treatment rat SCI model. The hDPSCs were isolated from human third molar. The cells were cultured in various concentrations of H2O2 (0-400 μM) at different time points (2-48 hours). Then, the cell viability was estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. In rat SCI model, 0.5×106 hDPSCs with and without H2O2 preconditioning were locally transplanted in contusion site. Behavioral assessment was rendered within 7 weeks after injection. Besides, the brain-derived neurotrophic factor (BDNF) was evaluated. The results of MTT showed that the cell viability rates were significantly lower at the higher concentrations of H2O2 at various times. However, the cell viability declined, followed by exposure to 50 μM H2O2 for a period of 2-12 hours compared with untreated cells. Basso, Beattie, and Bresnahan (BBB) findings revealed transplantation of the preconditioned hDPSCs increased BBB score significantly compared to the SCI group. Also, BDNF as a neurotrophic factor, dramatically increased in treated group. These finding suggest transplantation of sub-lethal concentration of H2O2 preconditioned hDPSCs would have protective roles through paracrine signals by enhancing survival capability transplanted cells which can cause motor neuron recovery in SCI.","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"429-437"},"PeriodicalIF":1.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13616756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0