{"title":"Anatomical variations of the hepatic veins: an observational study from a single cadaveric lab in South India.","authors":"Rajaneesh Shivamurthy Tolahunase, Pauline Shanthi, Suganthy Rabi","doi":"10.5115/acb.24.258","DOIUrl":"10.5115/acb.24.258","url":null,"abstract":"<p><p>Modern hepatic resections need to consider hepatic vein variations to reduce surgical complications. In this study we determined the variation in the branching pattern of hepatic veins by modified luminal casting technique and evaluated the association of the hepatic vein variations with morphological variations of the liver. The morphological features of thirty formalin-fixed livers were noted. The branching pattern of the hepatic veins was studied by retrograde injection of silicone into the hepatic veins through the inferior vena cava. According to the descriptions given by previous studies, the right, middle, left hepatic veins, and combined middle and left hepatic venous branching patterns were categorized. The data was analyzed statistically. The predominant hepatic vein patterns were De Cecchis type I right hepatic vein (30%), Neumann type I middle hepatic vein (67%), and Reichert type I left hepatic vein (70%). A common trunk for the middle and left hepatic vein was present in 60% and Wind's type II was more common (30%) followed by type I (20%) and type III (10%). While there was no association between the De Cecchis and Wind types, 90% of the conical-shaped livers exhibited type II middle/left hepatic vein pattern of Wind's classification. The present study also observed a rare variation of an accessory inferior left hepatic vein.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"365-372"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Devendra Shekhawat, Kristen Rizzuto, Rarinthorn Samrid, Chung Yoh Kim, Yoko Tabira, Kazzara Raeburn, Kathleen Bubb, Aaron S Dumont, Joe Iwanaga, Marios Loukas, Mahindra Kumar Anand, R Shane Tubbs
{"title":"Anatomical study of fascial and aponeurotic bands in the anterolateral leg.","authors":"Devendra Shekhawat, Kristen Rizzuto, Rarinthorn Samrid, Chung Yoh Kim, Yoko Tabira, Kazzara Raeburn, Kathleen Bubb, Aaron S Dumont, Joe Iwanaga, Marios Loukas, Mahindra Kumar Anand, R Shane Tubbs","doi":"10.5115/acb.24.273","DOIUrl":"10.5115/acb.24.273","url":null,"abstract":"<p><p>Foot drop can have debilitating effects on quality of life and is usually idiopathic. A better understanding of the nerve relationships of the anterior compartment of the leg could be important in treating some patients. Therefore, this study aimed to elucidate the deep fibular nerve and its relationship to various connective tissue bands along its course. Fifty-two cadaveric legs were dissected to reveal and identify the branching patterns of the common, superficial, and deep fibular nerves and their passage through the leg's posterior intermuscular septum (PIMS) and anterior intermuscular septum (AIMS). The oval passageway of the common fibular nerve was classified as the superior fibular band, and the crescentic passageways of the deep and superficial fibular nerves were classified as the middle and inferior fibular bands. The inferior boundary of the oval-shaped superior fibular band of the PIMS was positioned at the lateral aspect of the superior most region of the fibular neck. The crescentic middle fibular band of the AIMS was present in 96.15% of legs, its inferior boundary being consistently positioned in 98% of them. The other 2% presented with a thin band of connective tissue in the absence of a distinguishable AIMS. The crescentic inferior fibular band of the AIMS was present in 17.31% of legs. There were no significant differences between right and left sides in the presence or classifications of the fibular bands. Connective tissue bands along the course of the fibular nerves are common and should be considered in idiopathic palsies of these nerves.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"373-378"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anatomical variations of the infrahyoid muscles and ansa cervicalis: a systematic review and an updated classification system for the omohyoid muscle.","authors":"Nymfodora Malkidou, Vasileios Papadopoulos, Aliki Fiska","doi":"10.5115/acb.24.307","DOIUrl":"10.5115/acb.24.307","url":null,"abstract":"<p><p>The four infrahyoid muscles of the anterior neck are primarily innervated by the ansa cervicalis. This systematic review aims to evaluate the range of the anatomical variations in these muscles and their relationship to innervation patterns. A systematic search was conducted using PubMed and Google Scholar databases. Articles reporting variations in infrahyoid muscles and/or ansa cervicalis were independently evaluated following the PICOTS framework. The anatomical quality assessment tool was used to assess the quality of publications reporting anatomical variants. Seventy-seven studies, encompassing eighty-four cases, were included in the analysis. Of the 56-publication reporting infrahyoid muscle variations, 44 pertained to the omohyoid muscle (main or accessory), 3 to the sternohyoid, 4 to the sternothyroid, and 1 to the thyrohyoid, with no accessory variation observed in the latter. Atypical infrahyoid muscles were identified in 11 cases, 9 of which presented as levator glandulae thyroideae, and 2 as single cases. Variations in the ansa cervicalis were documented in 29 cases, only 1 case involved both ansa cervicalis and infrahyoid muscle variations. The extensive variability of the omohyoid muscle led to the development of a new classification system that integrates 4 types of consistency and 4 types of morphometric variations, providing valuable insights for clinical practice. The specialized use of the infrahyoid muscles in cancer staging, reconstruction after neck cancer surgery, and thyroid surgery underscores the need for a new framework to document their variations, particularly in the omohyoid muscle.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"322-333"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amira E Farage, Mona A Abdel-Kareem, Medhat Taha, Sara Abubakr, Nora Elshehawy Helal, Mahmoud Hendawy, Hanan A Elgendy, Ahmed Abdel-Monem Elmetwally, Hala Mahfouz, Tourki A S Baokbah, Mohammed R Rabei, Mohammad Akbar Hossain, Azza I Helal, Mohie Mahmoud Ibrahim
{"title":"Protective effect of <i>Chlorella vulgaris</i> against experimental hepatic ischemia reperfusion injury downregulating oxidative stress, inflammation, and apoptosis.","authors":"Amira E Farage, Mona A Abdel-Kareem, Medhat Taha, Sara Abubakr, Nora Elshehawy Helal, Mahmoud Hendawy, Hanan A Elgendy, Ahmed Abdel-Monem Elmetwally, Hala Mahfouz, Tourki A S Baokbah, Mohammed R Rabei, Mohammad Akbar Hossain, Azza I Helal, Mohie Mahmoud Ibrahim","doi":"10.5115/acb.24.318","DOIUrl":"10.5115/acb.24.318","url":null,"abstract":"<p><p>Hepatic ischemia/reperfusion (Hep I/R) is a great health burden during hepatic transplantation surgery. The present work aimed to examine the mitigative effect of <i>Chlorella vulgaris</i> against Hep I/R and its underlying protective mechanisms. The animals in the present research were classified into four equal experimental groups (n=6): the sham group, sham+<i>Chlorella vulgaris</i> group, Hep I/R group, and Hep I/R+<i>Chlorella vulgaris</i> group. Hepatic ischemia results in liver impairment, as evidenced by elevated liver enzyme levels and altered liver histology. It also reduced antioxidant enzyme levels and increased lipid peroxidation. Additionally, the Hep I/R group displayed significant suppression of the nuclear factor erythroid 2-related factor 2 (Nrf2)/haem oxygenase-1 pathway. There was a marked elevation in the expression of inflammatory markers, including nuclear factor kappa beta (NF-κB), tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and myloperoxidase, and NOD-like receptor protein 3 (NLRP3) and caspase-1. Furthermore, the levels of apoptotic markers such as caspase-3 and Bax were greater than those in the sham groups. Pretreatment with <i>Chlorella vulgaris</i> significantly protected against Hep I/R by reversing these effects. Rats pretreated with <i>Chlorella vulgaris</i> exhibited a hepatoprotective effect against Hep I/R through its inhibition of the NF-κB and NLRP3 cascades and Nrf2 stimulation.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"460-472"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histopathological changes and inflammatory and cell death pathways in the lungs of Balb/c mice with pneumonia induced by different concentrations of <i>Staphylococcus aureus</i>.","authors":"Celso Eduardo Silva Fortunato, Renata Pereira Alves, Karinne Spirandelli Carvalho Naves, Monica Cassel","doi":"10.5115/acb.24.304","DOIUrl":"10.5115/acb.24.304","url":null,"abstract":"<p><p>Pneumonia is often triggered by a bacterial infection, in many cases <i>Staphylococcus aureus</i>. Although this bacterium is found in the microbiota of healthy individuals, it can proliferate and release toxins in the respiratory tract, causing tissue damage by activating the inflammatory process and cell death pathways and resulting in serious complications. In this study, pneumonia was induced in Balb/c mice using different concentrations of <i>S. aureus</i> to evaluate histopathological changes and progression with increasing concentrations of colony forming units (CFUs) as well as their interactions with inflammatory and cell death markers. Hematoxylin and eosin histological techniques and peroxidase immunohistochemistry were utilized to investigate outcomes that included edema and disruption of the bronchiole and blood vessel walls. Alveolar collapse and bronchiolar hyperplasia were also analyzed and were statistically significant, but only hyperplasia varied between the two groups that received intermediate concentrations of CFU (10<sup>7</sup> and 10<sup>8</sup>, respectively) to induce pneumonia. In the immunohistochemical analysis, progression of apoptosis was observed in groups that received up to 10<sup>8</sup> CFU, along with a probable predominance of autophagy and reduction in IL-6 in the group that received the highest concentration (10<sup>9</sup> CFU). These characteristics appear to indicate an attempt to preserve and reuse cells when high CFU concentrations are present and eliminate infected cells at lower concentrations. The data from this present study contribute to understanding crosstalk between cell death pathways and the inflammatory response in <i>S. aureus</i>-induced pneumonia, and may assist in future intervention strategies.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"444-459"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphology and morphometry of pudendal nerve in East Indian population with surgical implications: a cadaveric study.","authors":"Grace Suganya, Biswabina Ray, Yashu Bharadwaj","doi":"10.5115/acb.25.012","DOIUrl":"10.5115/acb.25.012","url":null,"abstract":"<p><p>Pudendal nerve entrapment clinically has a wide spectrum of presentation ranging from urinary to anorectal and sexual dysfunction. The caliber of the pudendal nerve should be matched with calibre of donor nerve for a successful nerve transfer. Hence, we aimed at evaluating the morphology, dimensions of pudendal nerve at various sites of entrapment and certain trajectory distances to approach the pudendal nerve surgically. The study was conducted at Department of Anatomy at a tertiary care hospital and medical college. A detailed dissection of pudendal nerve was done and the morphology and morphometry of the pudendal nerve were studied in 10 cadavers with equal sex distribution (5 male and 5 female). Cross-sectional area (CSA) of the pudendal nerve at various sites of entrapment (piriformis, sacrospinous ligament, sacrotuberous ligament, and at Alcock's canal) and trajectory distance of pudendal nerve from various anatomical landmarks (ischial spine, sacral tuberosity, pubic symphysis, and inferior pubic ramus) were measured using digital vernier caliper. The CSA of pudendal nerve was more at piriformis (4.04 mm<sup>2</sup>) it decreased as the nerve travelled further to Alcock's canal (0.35 mm<sup>2</sup>). On comparison of the CSA and trajectory distances of the pudendal nerve with sex and sides there was no statistically significant difference. Morphologically, formation variation of pudendal nerve from S3 and S4 roots and trunk variations were also observed. These findings of the present study would help in various surgeries of perineum like nerve transfer, in placement of Richters stitch, transobturator tapes, pudendal nerve block etc.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"388-394"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Savvas Melissanidis, George Triantafyllou, George Botis, Konstantinos Natsis, Katerina Vassiou, Marianna Vlychou, George Tsakotos, Theodosis Kalamatianos, Nikolaos Lazaridis, George Matsopoulos, Maria Piagkou
{"title":"Morphology and morphometry of the vertebrobasilar system intracranial segment: a computed tomography angiography study.","authors":"Savvas Melissanidis, George Triantafyllou, George Botis, Konstantinos Natsis, Katerina Vassiou, Marianna Vlychou, George Tsakotos, Theodosis Kalamatianos, Nikolaos Lazaridis, George Matsopoulos, Maria Piagkou","doi":"10.5115/acb.24.249","DOIUrl":"10.5115/acb.24.249","url":null,"abstract":"<p><p>The current computed tomography angiography (CTA) study aimed to investigate the vertebrobasilar system (VBS) intracranial segment variant morphological anatomy and morphometry. Two hundred CTAs of 142 male and 58 female patients (with a mean age of 62.85±14.08) were retrospectively evaluated. Four hundred vertebral arteries (VAs) and 200 basilar arteries (BAs) were studied. In the V3 segment, the vessel's high riding course was identified in 10.8%. An arcuate foramen (AF) was identified surrounding the V3 segment in 12.0%. When AF was present, the V3 diameter was statistically significantly narrower (<i>P</i><0.001). The V4 segment was hypoplastic in 11.0% and fenestrated in one case (0.3%). The posterior inferior cerebellar artery typically originated from the V4 segment in 76.5%. On the BA morphology, we classified the vessel according to its inclination. The most common type was the J-shaped (55.5% of cases). The BA inclination was positively correlated with the vessel's length (<i>P</i>=0.002). The VBS intracranial segment was systematically investigated. Knowledge of the VBS typical and variant anatomy is essential for anatomists, radiologists, and clinicians. The most important findings was the following: (1) the AF presence significantly narrowed the VA diameter, which could lead to the vessel's compression, (2) the BA linear length (bending or inclination) positively correlated with the BA length, and (3) the V4 length was positively correlated with its diameter.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"379-387"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The developmental bases of cleft lip and cleft palate: cellular and molecular mechanisms.","authors":"Marcello Guarino","doi":"10.5115/acb.25.060","DOIUrl":"10.5115/acb.25.060","url":null,"abstract":"<p><p>Craniofacial development relies on proper growth and fusion during embryogenesis of initially distinct collections of mesenchyme derived from the cranial neural crest, covered by an epithelial lining of ectodermal origin. Fusion between these facial primordia implicates formation of an epithelial seam resulting from adherence and fusion between lining epithelia, and its subsequent removal to generate mesenchymal continuity. These embryonic processes involve a complex array of morphogenetic events requiring coordinated cell migration, survival, proliferation, death, patterning, adhesion, and differentiation, involving both the mesenchymal core and the primitive epithelial covering. Perturbation of any of these developmental events can lead to orofacial cleft phenotypes. Cleft lip and cleft palate are the most common congenital head deformities and, in general, among the commonest inborn defects. Indeed, due to the complexity of lip and palate development, the possibility of errors is a real event, therefore their relatively elevate frequency is not surprising. Understanding the pathogenesis of these malformations requires a thorough knowledge of the biological mechanisms underlying normal craniofacial embryogenesis and how they can be disturbed during development. An important contribution to our understanding of the fusion processes occurring in the orofacial district has come from studies on the role of the periderm in the adhesion between embryonic structures. This review summarises the normal morphogenesis of the upper lip/primary palate and secondary palate, as well as the mechanisms of aberrant development leading to cleft lip and palate, with particular attention to the role of the periderm, and cellular and molecular aspects of developmental pathogenesis.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"344-356"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aberrant rib cage anatomy with false ribs attachment to the sternum: review of the literature focused on slipping ribs syndrome case reports.","authors":"Sayed Mehrdad Azimi, Fateme Keshtparvar, Zahra Sadeghi, Hamid Bahramian","doi":"10.5115/acb.24.227","DOIUrl":"10.5115/acb.24.227","url":null,"abstract":"<p><p>The thoracic cage, formed by ribs and sternum, protects vital organs while enabling respiration. This review examines anatomical variations in rib-sternum attachments and their clinical significance through analysis of literature from 1993-2023. Variations, including bifid ribs (0.15%-3.4% prevalence) and cervical ribs (0.05%-3.0%), can lead to conditions like slipping rib syndrome (SRS). An extensive search of PubMed, Embase, Google Scholar, Web of Science, and specialized collections identified 17 comprehensive SRS case reports. Two independent reviewers evaluated these cases, revealing that rib-sternum morphological variations significantly impact respiratory biomechanics, with unstable costal cartilage identified as a primary cause of pain syndromes. These findings highlight the critical importance of recognizing aberrant rib-sternum anatomy for patient safety, particularly in thoracic procedures. Advanced imaging technologies have enhanced detection capabilities, allowing for improved surgical planning and patient management. This review underscores the value of both cadaveric and radiological examination in identifying these variations, and recommends further research to clarify their prevalence, functional implications, and clinical correlations to optimize treatment approaches and outcomes.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"334-343"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenshao Cai, Minyan Shi, David W Chan, Baohua Luo, Abudureyimujiang Ruze
{"title":"Challenging arterial patterns of renal and testicular arteries in a human cadaver: clinical significance and embryological bases revisited.","authors":"Wenshao Cai, Minyan Shi, David W Chan, Baohua Luo, Abudureyimujiang Ruze","doi":"10.5115/acb.24.336","DOIUrl":"10.5115/acb.24.336","url":null,"abstract":"<p><p>Typically, a kidney gets its arterial supply from a single renal artery, and the testicular arteries originate from the anterolateral surface of the abdominal aorta. The present case describes a variation in the origin of the testicular artery, which originates from a right accessory renal artery. We observed two renal arteries with the inferior vena cava sandwiched in between. The testicular artery originates from the inferior margin of the accessory renal artery, coursing down alongside the testicular vein into the right testis. During cephalic migration in embryogenesis, the conjunction of the ascending kidney and the descending testis is considered an important factor contributing to variations in the number and location of the vasculature in the kidneys or the gonads. An elaborate description of these variations is clinically significant for surgical interventions, renal transplants, renal and testicular imaging, and pathology.</p>","PeriodicalId":7831,"journal":{"name":"Anatomy & Cell Biology","volume":" ","pages":"478-482"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}