Journal of ultrastructure and molecular structure research最新文献_第4页

Effects of clofibrate treatment and of starvation on peroxisomes, mitochondria, and lipid droplets in mouse hepatocytes: A morphometric study 氯贝特治疗和饥饿对小鼠肝细胞过氧化物酶体、线粒体和脂滴的影响:一项形态计量学研究

Journal of ultrastructure and molecular structure research Pub Date : 1989-04-01 DOI: 10.1016/0889-1605(89)90037-2

Johan Meijer , Björn A. Afzelius

{"title":"Effects of clofibrate treatment and of starvation on peroxisomes, mitochondria, and lipid droplets in mouse hepatocytes: A morphometric study","authors":"Johan Meijer , Björn A. Afzelius","doi":"10.1016/0889-1605(89)90037-2","DOIUrl":"10.1016/0889-1605(89)90037-2","url":null,"abstract":"<div><p>Adult male mice of the NMRI strain were treated with a diet containing 0.5% clofibrate for 4 days to study its effects on peroxisomes, mitochondria, and lipid droplets in hepatocytes. Animals were also starved overnight to study the additional effects of starvation. Starvation of control animals had small effects on peroxisomes while the mitochondria became enlarged and occupied more of the cytoplasm. The number and fractional area of lipid droplets increased fivefold. Clofibrate treatment caused a doubling in number and average size of peroxisomes. No significant effects were observed in the number of mitochondrial profiles or lipid droplets although the size of the latter decreased to a third the value of the fed control. Starvation of clofibrate-treated animals led to a slight increase in the number of peroxisomes although their average size decreased by 30%. Mitochondrial average area increased and their fractional cytoplasmic area increased despite the decrease in numerical density. The number of lipid droplets increased twofold compared to that of clofibrate-treated animals while the size was not affected.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 1","pages":"Pages 87-94"},"PeriodicalIF":0.0,"publicationDate":"1989-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90037-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13762276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

An elementary information processing component in the circuitry of the retina generating the on-responses 视网膜电路中产生感应的基本信息处理组件

Journal of ultrastructure and molecular structure research Pub Date : 1989-04-01 DOI: 10.1016/0889-1605(89)90029-3

Fritiof S. Sjöstrand

{"title":"An elementary information processing component in the circuitry of the retina generating the on-responses","authors":"Fritiof S. Sjöstrand","doi":"10.1016/0889-1605(89)90029-3","DOIUrl":"10.1016/0889-1605(89)90029-3","url":null,"abstract":"<div><p>A pattern of neural connections that is a compulsory feature of photoreceptor terminals and is referred to as the synaptic ribbon complex was analyzed, and by combination of the structural information and information gained by intracellular recordings from photoreceptors, horizontal cells, and bipolar cells, it is possible to explain how the hyperpolarizing effect of light stimulating the photoreceptors is changed to a depolarization of depolarizing bipolar cells. The sign reversal is accomplished by the hyperpolarizing action of the horizontal cells on the photoreceptors, which blocks the transmission between the photoreceptors and the bipolar cells. This blocking action is controlled by the photoreceptor and it functions like a gate that is opened only when the photoreceptor is stimulated by light. The synaptic ribbon complex offers an example of an elementary information processing component with three input channels to the bipolar cells with each channel contributing a different piece of information and with the processing occurring presynaptically. Additional processing of information occurs within the dendritic tree through interactions of the responses of the individual dendritic endings to different types of input. This interaction can involve partial blocking of the conduction within the dendritic tree, making the interaction considerably more complex than simple summation. The responses recorded intracellularly from neurons reveal only the end result of the processing of information at the level of that neuron.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 1","pages":"Pages 24-38"},"PeriodicalIF":0.0,"publicationDate":"1989-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90029-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13836333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Interactions between pepper ringspot virus and cylindrical inclusions of two potyviruses 辣椒环斑病毒与两种波蒂病毒圆柱形包涵体的相互作用

Journal of ultrastructure and molecular structure research Pub Date : 1989-04-01 DOI: 10.1016/0889-1605(89)90032-3

Willem G. Langenberg , Dan E. Purcifull

{"title":"Interactions between pepper ringspot virus and cylindrical inclusions of two potyviruses","authors":"Willem G. Langenberg , Dan E. Purcifull","doi":"10.1016/0889-1605(89)90032-3","DOIUrl":"10.1016/0889-1605(89)90032-3","url":null,"abstract":"<div><p>Immunoelectron microscopy showed that cylindrical inclusions (CI) of the potyvirus potato virus Y (PVY) bound in addition to their homologous virions those of a co-infecting rod-shaped virus, pepper ringspot virus (PRV), in infected <em>Nicotiana benthamiana</em> leaf cells. The latter virus does not code for cylindrical inclusions and is cl assified as a Tobravirus. Virions of PRV were scattered throughout the cell cytoplasm and not associated with mitochondria in PVY + PRV double infections. Binding of PRV to mitochondria was disrupted in PVY + PRV infected cells. In double infections with a second potyvirus, tobacco etch virus (TEV), and PRV in <em>N. benthamiana</em> cells, TEV-CI bound homologous TEV virions but did not bind PRV. In contrast to PVY + PRV infections, virions of PRV attached end-on to mitochondrial limiting membranes in PRV-only and in TEV + PRV double infections. The results are interpreted to mean that there are differences in the PRV virion binding sites of PVY-CI and TEV-CI. In previous reports, potyviral CI have been nondiscriminating in binding virions or capsid proteins of other co-infecting rod-shaped viruses.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 1","pages":"Pages 53-58"},"PeriodicalIF":0.0,"publicationDate":"1989-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90032-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53919059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Membrane systems in endocytobiosis I. Specializations of the vacuolar membrane in bacteriocytes of Blattella germanica (L.) (Dictyoptera: Blattellidae) 内吞作用中的膜系统I.德国小蠊细菌细胞空泡膜的特化(双翅目:小蠊科)

Journal of ultrastructure and molecular structure research Pub Date : 1989-04-01 DOI: 10.1016/0889-1605(89)90034-7

E. Bigliardi , M.G. Selmi , B. Baccetti , L. Sacchi , A. Grigolo , U. Laudani

引用次数: 9

The ciliary necklace—A transmission electron microscopic study using tannic acid-containing fixation 纤毛项链-含单宁酸固定的透射电镜研究

Journal of ultrastructure and molecular structure research Pub Date : 1988-11-01 DOI: 10.1016/0889-1605(88)90011-0

Chikao Torikata

引用次数: 13

DNA bending induced by specific interaction of decamer binding proteins with immunoglobulin gene control sequences 十聚体结合蛋白与免疫球蛋白基因控制序列特异性相互作用诱导的DNA弯曲

Journal of ultrastructure and molecular structure research Pub Date : 1988-11-01 DOI: 10.1016/0889-1605(88)90004-3

Christine Brack, Georges Martin, Thomas Giller , Luzia Brunner

{"title":"DNA bending induced by specific interaction of decamer binding proteins with immunoglobulin gene control sequences","authors":"Christine Brack, Georges Martin, Thomas Giller , Luzia Brunner","doi":"10.1016/0889-1605(88)90004-3","DOIUrl":"10.1016/0889-1605(88)90004-3","url":null,"abstract":"<div><p>In order to investigate the properties of specific DNA-binding proteins involved in tissue-specific regulation of immunoglobulin genes, we have analyzed the interaction of nuclear proteins from mouse B-cell hybridomas with promoter and enhancer sequences of a mouse immunoglobulin heavy chain gene. Visualization of specific complexes has shown that protein binding induces a sharp bend at the position of the conserved decamer sequence. After fractionation of nuclear extracts, several sequence-specific DNA binding proteins could be distinguished by UV crosslinking to radioactive synthetic oligonucleotides. Decamer binding factor I (DBF-I), a protein of 100–105 kDa, and DBF-II, a family of proteins of 25–30 kDa, were purified on specific DNA-affinity columns. Both proteins bend the DNA at the dc sequence, as shown by electron microscopy and by gel retardation. These data suggest that one possible function of sequence-specific regulatory proteins may be to locally change the DNA topology, thereby facilitating the interaction of additional transcription factors with the primary complex.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"101 2","pages":"Pages 145-158"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90004-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14282147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Epididymal white adipose tissue after cold stress in rats I. Nonmitochondrial changes 大鼠冷应激后附睾白色脂肪组织的变化1 .非线粒体变化

Journal of ultrastructure and molecular structure research Pub Date : 1988-11-01 DOI: 10.1016/0889-1605(88)90001-8

Dragutin Lonçar, Björn A. Afzelius, Barbara Cannon

{"title":"Epididymal white adipose tissue after cold stress in rats I. Nonmitochondrial changes","authors":"Dragutin Lonçar, Björn A. Afzelius, Barbara Cannon","doi":"10.1016/0889-1605(88)90001-8","DOIUrl":"10.1016/0889-1605(88)90001-8","url":null,"abstract":"<div><p>Epididymal adipose tissue in the rat is generally considered to be “pure” white adipose tissue (WAT) with a characteristic structure and function. Previous studies in cats have, however, indicated that adipose tissue with the morphological appearance of WAT could be converted into a tissue with the morphological appearance of brown adipose tissue (BAT) by intermittent cold stress. The present electron microscopic and morphometric study describes the effect of intermittent cold stress on the epididymal WAT of young rats. The tissue volume decreased markedly as did the lipid content. The mitochondrial volume increased dramatically. The extracellular matrix was vastly reduced as was the thickness of the plasma membrane, and the number of gap junctions between adipocytes increased markedly. Indications of neoinnervation and neovascularization were observed. A great abundance of preadipocytes indicated proliferative activity of the endothelium. The low amount of lipid droplets and a relative abundance of smooth and rough endoplasmic reticulum, Golgi apparatus, and lysosomes in the epididymal WAT of cold-stressed rats gave the cells the morphological appearance of young adipocytes or preadipocytes, whereas the hypertrophic and hyperplastic mitochondria, the relative paucity of ribosomes on lipid droplet membranes, and the increased innervation and vascularization gave the cells the morphological characteristics of brown adipose tissue.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"101 2","pages":"Pages 109-122"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90001-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14398879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Effects of pressure on vesicle size, density, and distribution in the canine carotid arterial endothelium 压力对犬颈动脉内皮小泡大小、密度和分布的影响

Journal of ultrastructure and molecular structure research Pub Date : 1988-11-01 DOI: 10.1016/0889-1605(88)90014-6

Mary M.L. Lee , George B. Schuessler , Kevin Lau , Shu Chien

{"title":"Effects of pressure on vesicle size, density, and distribution in the canine carotid arterial endothelium","authors":"Mary M.L. Lee , George B. Schuessler , Kevin Lau , Shu Chien","doi":"10.1016/0889-1605(88)90014-6","DOIUrl":"10.1016/0889-1605(88)90014-6","url":null,"abstract":"<div><p>The purpose of the present experiment was to study the effects of internal hydrostatic pressure on vesicle size, density, and distribution in the canine carotid arterial endothelium by transmission electron microscopy. The pressures applied in this study were 0 (control), 40, 60, 80, 100, and 150 mm Hg. The results of transmission electron microscopy and computer analysis on the plasmalemmal vesicles of aortic endothelium showed that luminal, abluminal, and junctional vesicles all increased their diameter as the pressure was raised from 0 mm Hg, reaching a maximum at 80 mm Hg, and then decreased in size with further increases in pressure to 150 mm Hg. There was a significant difference in diameter among vesicles in different regions of the endothelium, with the diameter of luminal vesicles larger than those of abluminal and junctional vesicles. The densities of vesicles showed very little change from 0 to 80 mm Hg; but they increased markedly as the pressure was further raised from 80 to 150 mm Hg. These results indicate that pressure is an important mechanical factor governing the size and density of plasmalemmal vesicles in aortic endothelium.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"101 2","pages":"Pages 236-242"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90014-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14398886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Author index for volume 101 第101卷的作者索引

Journal of ultrastructure and molecular structure research Pub Date : 1988-11-01 DOI: 10.1016/0889-1605(88)90016-X

引用次数: 0

Epididymal white adipose tissue after cold stress in rats II. Mitochondrial changes 冷应激对大鼠附睾白色脂肪组织的影响[j]。线粒体的变化

Journal of ultrastructure and molecular structure research Pub Date : 1988-11-01 DOI: 10.1016/0889-1605(88)90010-9

Dragutin Loncˇar, Björn A. Afzelius, Barbara Cannon

{"title":"Epididymal white adipose tissue after cold stress in rats II. Mitochondrial changes","authors":"Dragutin Loncˇar, Björn A. Afzelius, Barbara Cannon","doi":"10.1016/0889-1605(88)90010-9","DOIUrl":"10.1016/0889-1605(88)90010-9","url":null,"abstract":"<div><p>We have previously shown that after severe cold stress of rats the epididymal white adipose tissue (WAT) acquires many of the morphological characteristics (innervation, vascularization, appearance of adipocytes) of brown adipose tissue (BAT). In the present study, the mitochondrial characteristics have been analyzed. Mitochondria from the epididymal fat pad of cold-stressed rats become enlarged and had a volume 1.7 times greater than that in the control group (0.5 μm<sup>3</sup> in the experimental versus 0.3 μm<sup>3</sup> in the control group). The mitochondria occupied about 60% of the cytoplasm (14% in the control group), thus approaching the highest value reported for any cell. The straight or slightly wavy cristae completely traversed the width of the mitochondria and occupied an area of 26 μm<sup>2</sup>/1 μm<sup>3</sup> of mitochondrion in the experimental adipose tissue (7 μm<sup>2</sup> in the control group) and 15 μm<sup>2</sup>/1 μm<sup>3</sup> of cytoplasm (1.4 μm<sup>2</sup> in the control). The mitochondrial matrix became electron lucid and could contain lamellar whorls, as could the surface of the mitochondria. These mitochondria of epididymal WAT resembled, both morphologically and morphometrically, mitochondria in the BAT of cold-exposed rodents. In the epididymal mitochondria, immunoelectron microscopy did not reveal the presence of the BAT-specific uncoupling protein thermogenin, nor could the slot-blot technique detect thermogenin mRNA. We conclude that even under these extreme conditions of cold stress, WAT cannot adopt the thermogenin-dependent thermogenesis of BAT. The dramatic mitochondriogenesis can be interpreted only as being indicative of an extremely high metabolism in the tissue, thus placing unprecedented pressure on the energy turnover capacity of the cell.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"101 2","pages":"Pages 199-209"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90010-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14282149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 94