Diagnostic and clinical immunology最新文献_第3页

Immune complexes in solid tumours precipitable by 3.5% polyethylene glycol: analysis of some nonspecific components. 3.5%聚乙二醇可沉淀实体瘤中的免疫复合物:一些非特异性成分的分析。

Diagnostic and clinical immunology Pub Date : 1988-01-01

L Guidi, R Baroni, C Bartoloni, M Pellegrino, A Tricerri, M Marciano, C Barone, G Gambassi

{"title":"Immune complexes in solid tumours precipitable by 3.5% polyethylene glycol: analysis of some nonspecific components.","authors":"L Guidi, R Baroni, C Bartoloni, M Pellegrino, A Tricerri, M Marciano, C Barone, G Gambassi","doi":"","DOIUrl":"","url":null,"abstract":"Some nonspecific components (IgG, C3c, C4,) of circulating immune complexes (IC) precipitable by 3.5% PEG were assayed by laser nephelometry in the sera of 71 patients with solid tumours, and of 39 patients with autoimmune diseases. PEG-IgG, PEG-C3c, and PEG-C4 were higher in cancer and in autoimmune diseases than in controls. In cancer patients, PEG-C4 levels and PEG-C4 positivity rate correlated well with tumour burden. PEG-C3c and PEG-IgG were present at higher levels and PEG-C4 was present at lower concentration in cancer than in autoimmune diseases. The results of the present study, performed on a large population of patients with cancer and autoimmune diseases, indicate a different composition of circulating IC in these diseases. Particularly, these data suggest a preferential involvement of the alternative pathway of complement activation in cancer, as already described by other authors. The aggregation of C3c to IC, mediated by the alternative pathway, seems to be the \"key\" event in the process of IC solubilization. In cancer patients' sera, the presence of \"solubilized\" IC could partly explain their peculiar biological behaviour as well as the disagreement observed among the different assay methods based upon the binding with complement factors.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"284-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14349232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow cytometric evaluation of leukocyte function. 流式细胞术评价白细胞功能。

Diagnostic and clinical immunology Pub Date : 1988-01-01

G T Stelzer, J P Robinson

{"title":"Flow cytometric evaluation of leukocyte function.","authors":"G T Stelzer, J P Robinson","doi":"","DOIUrl":"","url":null,"abstract":"The marriage of monoclonal antibody technology and flow cytometry has provided clinical researchers with a powerful tool for the characterization of leukocytes from various sources. In this regard, flow cytometry has been primarily used for the immunophenotyping of peripheral blood lymphocytes in leukemias and various immunodeficiency disorders. Flow cytometry is also useful for the evaluation of leukocyte function in vitro and in vivo. This review discusses the various applications of flow cytometry for the assessment of leukocyte function. Since several cell surface antigens are important constituents involved in cell function, immunofluorescence identification of these markers can provide significant information regarding cell function. Analysis of activation antigen expression by monoclonal antibodies and flow cytometry can provide significant insights about the presence of functional leukocyte populations in patients. Flow cytometry can also be used to directly analyze leukocyte function. Procedures for the quantitative flow cytometric analysis of proliferation of activated lymphocyte subsets are reviewed. Early cell activation is also amenable to flow cytometric measurement. Early activation events such as alterations in membrane potential, intracellular free calcium redistribution, intracellular pH, and changes in membrane fluidity, as well as the direct measurement of enzyme activity, are also described.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 5","pages":"223-31"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14409793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lymphocyte phenotyping of infants with congenital heart disease: comparison of cell preparation techniques. 婴儿先天性心脏病的淋巴细胞表型:细胞制备技术的比较

Diagnostic and clinical immunology Pub Date : 1988-01-01

H B Slade, J H Greenwood, J L Hudson, R H Beekman, M C Riedy, S A Schwartz

{"title":"Lymphocyte phenotyping of infants with congenital heart disease: comparison of cell preparation techniques.","authors":"H B Slade, J H Greenwood, J L Hudson, R H Beekman, M C Riedy, S A Schwartz","doi":"","DOIUrl":"","url":null,"abstract":"The diagnostic evaluation of infants with suspected DiGeorge syndrome includes peripheral blood lymphocyte phenotype analysis by flow cytometry. Mononuclear cells are typically concentrated from infants' blood samples by Ficoll-Hypaque (FH) density gradient centrifugation prior to monoclonal antibody (Mab) staining. Having observed that lymphocyte percentages in samples prepared by this technique were low more often than anticipated based on the prevalence of the suspected diagnosis, we undertook a prospective study of 55 infants with congenital heart disease comparing FH with a whole-blood (WB) technique. Sixty healthy adult controls were similarly studied. We report the observation that FH-prepared mononuclear cells from blood samples of infants less than 4 months of age yield substantially different phenotypes than WB-prepared samples. The differences are related to red blood cell (RBC) contamination. No such difference was seen with adult samples. Unlike FH-prepared adult samples, contaminating RBCs are indistinguishable from lymphocytes through at least 4 months of age when assessed by the standard cytometric gating parameters of forward and 90 degrees light scatter. The use of a WB ammonium chloride lysis technique is recommended as most appropriate for the preparation of infants' blood samples.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 5","pages":"249-55"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14486946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depression by Fc gamma receptor ligands of SRBC-induced IgM-PFC generation in human blood mononuclear cell cultures. Fc受体配体抑制srbc诱导的人血单核细胞IgM-PFC生成。

Diagnostic and clinical immunology Pub Date : 1988-01-01

A Gabrielli, R Silver, G Teti, M S Chiofalo, A Misefari, D Venza-Teti, G Virella, M F La Via

{"title":"Depression by Fc gamma receptor ligands of SRBC-induced IgM-PFC generation in human blood mononuclear cell cultures.","authors":"A Gabrielli, R Silver, G Teti, M S Chiofalo, A Misefari, D Venza-Teti, G Virella, M F La Via","doi":"","DOIUrl":"","url":null,"abstract":"A tissue-culture system to stimulate human peripheral blood mononuclear cells (PBMC) has been employed in which IgM plaque-forming cell (IgM PFC) generation in response to sheep erythrocytes (SRBC) is dependent on macrophages and T suppressor and helper lymphocytes. In this system PBMC from normal subjects give IgM PFC responses ranging from 26 to 938 PFC/culture. Heat-aggregated human IgG or immune complexes present for the duration of culture induce a significant depression of PFC. Unaggregated IgG has no effect on the response or only a moderate stimulatory effect at the highest dose. The results of these experiments are compatible with previous results in a murine system, which indicated that Fc gamma receptor-positive (Fc gamma R+) cells in the suppressor subset are the target for aggregated IgG and induce depression of the PFC response. A similar mechanism may be operating in the human system described here, although the target cell has not been identified. These results may reflect a mechanism of immunomodulation dependent on interaction of Fc receptor (FcR) ligands with FcR, which may play a role in the pathogenesis of immune complex disorders.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"304-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14109349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of platelet-associated immunoglobulin in immune thrombocytopenia by flow cytometry. 流式细胞术检测免疫性血小板减少症患者血小板相关免疫球蛋白。

Diagnostic and clinical immunology Pub Date : 1988-01-01

M C Heim, B H Petersen

引用次数: 0

Natural history of chronic granulomatous disease. 慢性肉芽肿病的自然史。

Diagnostic and clinical immunology Pub Date : 1988-01-01

N Kamani, S D Douglas

引用次数: 0

Interleukin-2 receptor is functionally linked with in vitro activation of B cells isolated from non-Hodgkin's lymphomas. 白细胞介素-2受体在功能上与非霍奇金淋巴瘤分离的B细胞的体外活化有关。

Diagnostic and clinical immunology Pub Date : 1988-01-01

O Garraud, E LeGrand, H Eghbali, G Hoerni-Simon, B Hoerni, B Guillemain