Diagnostic and clinical immunology最新文献_第2页

Mononuclear cells from rheumatoid arthritis patients exhibit a depressed IgM response to sheep erythrocyte stimulation in vitro. 类风湿性关节炎患者的单核细胞对绵羊红细胞的体外刺激表现出抑制的IgM反应。

Diagnostic and clinical immunology Pub Date : 1988-01-01

A Gabrielli, R Silver, G Teti, M S Chiofalo, A Misefari, D Venza-Teti, G Virella, M F La Via

引用次数: 0

An immunological procedure for the rapid identification of Phaeoannellomyces werneckii. 一种快速鉴定卫氏褐霉的免疫学方法。

Diagnostic and clinical immunology Pub Date : 1988-01-01

P G Standard, L Kaufman, A A Padhye

引用次数: 0

Immunoresponsiveness in hemophilia: lymphocyte- and phagocyte-mediated functions. 血友病的免疫反应性:淋巴细胞和吞噬细胞介导的功能。

Diagnostic and clinical immunology Pub Date : 1988-01-01

S Antonaci, E Jirillo, D Stasi, V De Mitrio, M F La Via, L Bonomo

{"title":"Immunoresponsiveness in hemophilia: lymphocyte- and phagocyte-mediated functions.","authors":"S Antonaci, E Jirillo, D Stasi, V De Mitrio, M F La Via, L Bonomo","doi":"","DOIUrl":"","url":null,"abstract":"Several deficits of immune response have been reported in hemophiliacs. In order to evaluate the overall immune function in this disease, we have investigated peripheral blood mononuclear cells (PBMC) surface phenotype, lymphokine production, B cell responsiveness, monocyte- and polymorphonuclear (PMN) cell-mediated responses in a group of 25 A or B hemophiliac patients. They were treated with concentrates (greater than 30,000 U/year) for at least 8 years, and ten of them were positive for human immunodeficiency virus (HIV) infection. With particular reference to lymphocyte surface markers, a low CD4+/CD8+ ratio and an increased frequency of CD25+ and surface immunoglobulin (sIg) positive lymphocytes were noted in HIV+ subjects. By contrast, CD3+ and CD4+ cell frequency was decreased in HIV- patients, whereas in the same individuals CD11+ and sIg+ cell percentage was augmented. Regardless of HIV infection, a quite variable degree of B-cell response was seen in hemophiliacs with either a T-independent or a T-dependent polyclonal B cell activator. PMN chemotaxis, phagocytosis, and killing were significantly diminished, whereas similar monocyte functions were basically unaffected. Finally, hemophiliacs were characterized by a profound impairment of leukocyte inhibiting factor (LIF) and lymphocyte-derived chemotactic factor (LDCF) production. Our findings clearly indicate an impairment of immune function in hemophiliacs regardless of HIV infection.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"318-25"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14349069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibodies to histones in rheumatoid arthritis. 类风湿关节炎组蛋白抗体。

Diagnostic and clinical immunology Pub Date : 1988-01-01

Y Muzellec, P Le Goff, J Jouquan, P Fauquert, S Muller, P Youinou

引用次数: 0

Utility of frozen platelets for a platelet antibody assay using flow cytometric analysis. 利用流式细胞术分析，冷冻血小板用于血小板抗体测定。

Diagnostic and clinical immunology Pub Date : 1988-01-01

J Lazarchick, P C Das, T J Jones, R J Russell, S A Hall

{"title":"Utility of frozen platelets for a platelet antibody assay using flow cytometric analysis.","authors":"J Lazarchick, P C Das, T J Jones, R J Russell, S A Hall","doi":"","DOIUrl":"","url":null,"abstract":"The immunoreactivity of a PLA1 antibody-containing serum to frozen/thawed platelets prepared by four different procedures was measured to assess the practicality of preparing a platelet reagent that could be easily stored and readily used in an immunofluorescent platelet antibody detection system. These frozen aliquots were analyzed at intervals up to 23 weeks after initial freezing and storage. For analysis, the frozen platelets were thawed, washed, resuspended to 2.5 x 10(5) platelets/mL and incubated with a dilution of fresh autologous serum that lacked platelet antibody or with a similar dilution of frozen human serum containing IgG anti-PLA1 antibody. At each interval, a freshly drawn platelet sample from the same donor was incubated in the same manner and used for comparison. All platelet mixtures were then washed and incubated with fluorescein conjugated goat F (ab')2 antihuman IgG. After repeat washing, each mixture was then analyzed with a flow cytometer for the extent of fluorescent antibody bound to each platelet mixture. Anti-PLA1 antibody reactivity with either the fresh or the frozen/thawed platelets remained stable over the period of analysis, with no significant difference in immunofluorescence with either fresh or frozen platelets as target cells. Platelet recovery following the thawing step ranged from 12% to 36% and was independent of the storage time. These studies suggest that frozen platelets can be readily used as reagents for platelet antibody assays.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"338-43"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14349072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interrelations of lymphocyte subset values, human immunodeficiency virus antibodies, and HIV antigen levels of homosexual males in San Francisco. 旧金山同性恋男性淋巴细胞亚群值、人类免疫缺陷病毒抗体和HIV抗原水平的相互关系

Diagnostic and clinical immunology Pub Date : 1988-01-01

J F Krowka, D P Stites, A R Moss, C H Casavant, J R Carlson, J C Chermann, F Barre-Sinoussi, R P Rodgers, P Bacchetti, T M McHugh

{"title":"Interrelations of lymphocyte subset values, human immunodeficiency virus antibodies, and HIV antigen levels of homosexual males in San Francisco.","authors":"J F Krowka, D P Stites, A R Moss, C H Casavant, J R Carlson, J C Chermann, F Barre-Sinoussi, R P Rodgers, P Bacchetti, T M McHugh","doi":"","DOIUrl":"","url":null,"abstract":"Serum and leukocytes from a cohort of homosexual males were analyzed to determine the interrelationships of antibodies to human immunodeficiency virus (HIV), serum HIV antigen levels, and phenotypical differences in lymphocyte subpopulations of HIV antibody-positive (HIV Ab+) and HIV antibody-negative (HIV Ab-) homosexual males. Significant reductions were observed in the percentages of B lymphocytes, CD4+ and CD4+ kappa lambda- T lymphocytes and the CD4+/CD8+ ratios of HIV Ab+ homosexual males in comparison to HIV Ab- homosexual males. Significant increases were observed in the percentages of CD8+, CD8+ CD11b-, CD8+ kappa lambda-, CD8+ DR+, CD8+Leu7+, and Leu7+ lymphocytes of HIV Ab+ study subjects. Statistical analysis revealed that among the immunological variables tested, decreases in the CD4+/CD8+ ratio and in the percentage of CD4+ kappa lambda- lymphocytes showed the strongest associations with HIV-sero-positivity in asymptomatic homosexual males. Only 44 (16.5%) of 267 HIV Ab+ homosexual males had detectable levels of HIV antigen (HIV Ag) in their serum. The percentages of CD4+ or CD4+ kappa lambda- lymphocytes and the CD4+/CD8+ ratios of HIV Ab+ males differed significantly between HIV Ag-positive (HIV Ag+) and--negative (HIV Ag-) homosexual males. These variables, however, did not correlate well with HIV Ag levels,indicating that no clear associations can be drawn between levels of HIV antigen and lymphocyte subset abnormalities of HIV-infected individuals.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"381-7"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14349074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence intensity of immunostained cells as a diagnostic aid in lymphoid leukemias. 免疫染色细胞的荧光强度作为淋巴性白血病的诊断辅助。

Diagnostic and clinical immunology Pub Date : 1988-01-01

C W Caldwell, W P Patterson

{"title":"Fluorescence intensity of immunostained cells as a diagnostic aid in lymphoid leukemias.","authors":"C W Caldwell, W P Patterson","doi":"","DOIUrl":"","url":null,"abstract":"The standardized fluorescence intensity (FI) of immunostained normal and malignant B-cells was measured as a method of evaluating cellular heterogeneity between lymphoid malignancies. Mature B-cells from peripheral blood lymphocytes (PBL) of different individuals demonstrated characteristic peaks of FI when specific monoclonal antibodies (MoAbs) were employed in standard flow cytometric procedures. Malignant cells from patients with lymphoid leukemias demonstrated FI that differed from that of normal B-cells with various MoAbs and that differed among categories of leukemia. By using a panel of MoAbs reactive with B-cells, as well as a T200 (CD45) antigen, a scheme of malignant cell differentiation may be produced that approximates stages of normal B-cell differentiation. When the FI of malignant cells differs from that of normal cells, or when atypical peaks (or additional peaks) of FI are present in flow cytometric histograms, the investigator should be alerted to the probability of abnormal cell populations. In addition, it is frequently possible to use this information to help classify malignancies, thereby contributing to identification of small numbers of malignant B-cells in otherwise equivocal situations.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"371-6"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14393815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunocytochemical localization of cytoskeletal antigens in KB and HEp-2 cells. KB和HEp-2细胞中细胞骨架抗原的免疫细胞化学定位。

Diagnostic and clinical immunology Pub Date : 1988-01-01

A S Price, L B Keil, V A DeBari

引用次数: 0

Experimental drug-induced immune thrombocytopenia in monkeys. 猴子实验性药物性免疫性血小板减少症。

Diagnostic and clinical immunology Pub Date : 1988-01-01

B H Petersen, M C Heim, J F White

引用次数: 0

Platelet antibody detection using frozen pooled human lymphocyte antigen-typed platelets as target cells. 血小板抗体检测用冷冻池人淋巴细胞抗原型血小板作为靶细胞。

Diagnostic and clinical immunology Pub Date : 1988-01-01

J Lazarchick, T J Jones, R J Russell, S A Hall

{"title":"Platelet antibody detection using frozen pooled human lymphocyte antigen-typed platelets as target cells.","authors":"J Lazarchick, T J Jones, R J Russell, S A Hall","doi":"","DOIUrl":"","url":null,"abstract":"The immunoreactivity of five sera containing either iso-, auto- or allo-antiplatelet antibodies to frozen-thawed human lymphocyte antigen (HLA)-typed pooled platelets was assessed to determine the practicality of using such a platelet preparation in an immunofluorescent platelet antibody system. The platelet reagent was analyzed over an 8-week period after initial freezing and storage. Mixtures of platelets with each serum or dilutions of it were initially incubated for 1 hour, then extensively washed and incubated with fluorescein-conjugated goat F(ab')2 antihuman IgG, A, M. After repeat washing, each mixture was then analyzed using a flow cytometer to determine the relative amount of fluorescent antibody bound. Not only was each of the five sera consistently positive for the presence of an anti-platelet antibody, but the extent of immunoreactivity for each remained relatively stable over the 8-week period of analysis. The results suggest that frozen pooled HLA-typed platelets should be an invaluable reagent for screening serum samples for the presence of antiplatelet antibodies in patients who are thrombocytopenic or who are refractory to platelet transfusion therapy.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 6","pages":"377-80"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14349073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0