Experimental drug-induced immune thrombocytopenia in monkeys.

Diagnostic and clinical immunology Pub Date : 1988-01-01
B H Petersen, M C Heim, J F White
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Abstract

In these studies we report the induction, by 7,8-dimethoxy-1H-3-benzaepin-2-amine HCl, of immune thrombocytopenia in monkeys and its characterization by flow cytometry. Three of eight monkeys developed thrombocytopenia following administration of high doses (35 mg/kg) of the drug. The characteristics of the thrombocytopenia, including rapid induction on rechallenge with the drug, met the criteria indicative of drug-induced immune thrombocytopenia. Platelet surface-associated immunoglobulin could be detected by flow cytometry as early as 21 days before the onset of thrombocytopenia. A critical concentration of antibody on the platelet surface appeared to be necessary for elimination of the platelets from the blood. Evidence from experiments using the serum from an affected monkey suggested that drug interaction with the platelet surface produced a site for antibody binding, possibly a neoantigen, which required the continued presence of the drug.

猴子实验性药物性免疫性血小板减少症。
在这些研究中,我们报道了7,8-二甲氧基- 1h -3-benzaepin-2-胺HCl对猴子免疫血小板减少的诱导作用,并通过流式细胞术对其进行了表征。在给予高剂量(35 mg/kg)药物后,8只猴子中的3只出现了血小板减少症。血小板减少的特点,包括药物再挑战后快速诱导,符合药物性免疫性血小板减少的标准。血小板表面相关免疫球蛋白可在血小板减少症发病前21天通过流式细胞术检测到。血小板表面抗体的临界浓度似乎是血小板从血液中清除所必需的。使用受感染猴子血清的实验证据表明,药物与血小板表面的相互作用产生了抗体结合位点,可能是一种新抗原,这需要药物的持续存在。
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