{"title":"Mechanism of K562-induced human natural killer cell inactivation using highly enriched effector cells isolated via a new single-step sheep erythrocyte rosette assay","authors":"S.I. Abrams , Z. Brahmi","doi":"10.1016/0769-2625(88)90064-5","DOIUrl":"10.1016/0769-2625(88)90064-5","url":null,"abstract":"<div><p>In this study, we used preparations highly enriched in human natural killer (NK) cells to further characterize the mechanism of taget-cell-induced NK inactivation. Highly enriched populations of NK cells were obtained by a newly developed, single-step sheep red blood cell rosette assay. This method, which did not require any incubation steps to facilitate cell contact, permitted a rapid and efficient isolation if NK cells from adherent-cell-depleted peripheral blood lymphocytes. The non-rosetted cells had high NK activity, possessed large granular lymphocyte (LGL) morphology and expressed the NK-associated antigens Leu-11a, Leu-7, OKM1 and NKH-1. In contrast, the rosetted cells had significantly lower NK activity, possessed typical lymphocyte morphology and expressed the T-cell-associated marker OKT3.</p><p>Next, we examined the ability of these NK-enriched effector cells (EC<sub>c</sub>) to become inactivated by K562. Functional studies revealed that EC<sub>c</sub> lost <span><math><mtext>≥95%</mtext></math></span> of their lytic capacity following incubation with K562 at a ratio of 2/1 for 6 h. However, to achieve this level of inactivation, it was essential that the cell suspension be gently mixed every 90–120 min. Inactivation was not due to cell death and did not reflect changes in the percentages of cells bearing the Leu-11a, Leu-7, OKM1 and NKH-1 antigens, but was associated with an increase in cell surface concentration of OKM1. As judged by gross morphology, the percentages of LGL in EC<sub>c</sub> before and after treatment with K562 were essentially the same. Finally, K562-treated EC<sub>c</sub> also lost their ability to mediate antibody-dependent cellular cytotoxicity (ADCC), suggesting that both NK-cell-mediated cytotoxicity and ADCC may involve a common lytic pathway.</p></div>","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"Pages 361-381"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90064-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14297051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Understanding the biology of non-mhc-restricted cytotoxicity: toward new approaches in the treatment of cancer?","authors":"J M Michon, W H Fridman","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"433-5"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14391532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunopathologie de l'œil","authors":"","doi":"10.1016/0769-2625(88)90078-5","DOIUrl":"https://doi.org/10.1016/0769-2625(88)90078-5","url":null,"abstract":"","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"Page 480"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90078-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90129156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trois réunions sur le thème du cancer","authors":"","doi":"10.1016/0769-2625(88)90088-8","DOIUrl":"https://doi.org/10.1016/0769-2625(88)90088-8","url":null,"abstract":"","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"Page 3"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90088-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91773801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NK cells: A discrete leukocyte subset of still undefined origin mediating biologically relevant functions upon specific stimulation","authors":"B. Perussia, G. Trinchieri","doi":"10.1016/0769-2625(88)90069-4","DOIUrl":"10.1016/0769-2625(88)90069-4","url":null,"abstract":"","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"Pages 438-443"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90069-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14180639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Savagner, B. Bauvois, M.-A. Deugnier, B.A. Imhof, J.-P. Thiery
{"title":"Aspects of haemopoietic cell dynamics: Ontogeny and targeted migration","authors":"P. Savagner, B. Bauvois, M.-A. Deugnier, B.A. Imhof, J.-P. Thiery","doi":"10.1016/0769-2625(88)90067-0","DOIUrl":"10.1016/0769-2625(88)90067-0","url":null,"abstract":"<div><p>In the developing avian and mammalian embryo, haemopoietic cells appear first in transient foci whose function is restricted to discrete periods of embryogenesis. These foci are essentially represented by the yolk sac, intraembryonic dispersed foci and the liver. Haemopoietic cells then repopulate the developing spleen, thymus and bone marrow, organs which persist and develop after birth.</p><p>In the present review, we describe a number of possible mechanisms controlling specific adhesion, oriented migration and invasiveness of haemopoietic cells. One concerns the high specificity of the interactions of homing receptors on the surface of haemopoietic cells with determinants on vascular endothelium and/or thymic epithelium. A second is the importance of the presence of some macromolecules in the extracellular matrix, such as fibronectin, collagen, laminin and elastin. These components can interact with the haemopoietic cells (and/or induce chemotaxis) via the existence of specific receptors on the surface of the haemopoietic cells. Another mechanism is the activation of the haemopoietic cells through the interactions of cell-chemotactic factor, cell-extracellular matrix and/or cell-thymic epithelium. This activation can lead to: 1) the expression of new specific cell-surface receptors for the target foci; 2) the secretion of specific protease and glycosidase systems active upon the extracellular matrix; and 3) the differentiation of these cells in the thymus.</p></div>","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"Pages 409-431"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90067-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14180763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"VIIe symposium international sur l'allergie alimentaire","authors":"","doi":"10.1016/0769-2625(88)90090-6","DOIUrl":"https://doi.org/10.1016/0769-2625(88)90090-6","url":null,"abstract":"","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":"139 4","pages":"Page 3"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90090-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90125650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}