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Oncogene research最新文献

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The chicken cellular progenitor of the v-ets oncogene, p68c-ets-1, is a nuclear DNA-binding protein not expressed in lymphoid cells of the spleen. 鸡v-ets癌基因的细胞祖蛋白p68c-ets-1是一种核dna结合蛋白，在脾脏淋巴样细胞中不表达。
Oncogene research Pub Date : 1990-01-01
D Leprince, J C Gesquiere, D Stehelin
{"title":"The chicken cellular progenitor of the v-ets oncogene, p68c-ets-1, is a nuclear DNA-binding protein not expressed in lymphoid cells of the spleen.","authors":"D Leprince,&nbsp;J C Gesquiere,&nbsp;D Stehelin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The chicken cellular c-ets-1 locus encodes for two related proteins generated by alternative splicing: the widely expressed p54c-ets-1 protein and the cellular homolog of the v-ets-encoded domain of the E26-transforming protein P135gag-myb-ets, p68c-ets-1 which has been found so far only in the spleen. We have prepared a new site specific antiserum directed against the amino-terminus of p68c-ets-1, which is highly hydrophobic by contrast to the hydrophilic NH2 terminus of p54c-ets-1. This antiserum specifically immunoprecipitated p68c-ets-1 and P135gag-myb-ets only in denaturing and reducing conditions. Despite these biochemical differences at their amino-terminal parts, p68c-ets-1, as p54c-ets-1, is a nuclear protein able to bind to DNA in vitro. Unlike p54c-ets-1 which is expressed at high levels in T- and B-lymphoid cells, p68c-ets-1 is not expressed in lymphoid cells of the spleen. Thus, the two c-ets-1 encoded proteins, although both exhibiting DNA-binding properties in vitro, display differences both in the nature of their specific NH2 termini, and in their level and pattern of expression.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"255-65"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13356213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The beta-interferon-mediated antimitogenic state resembles the anti-viral state. 干扰素介导的抗生丝状态类似于抗病毒状态。
Oncogene research Pub Date : 1990-01-01
M A Garcia-Blanco, J A Porter, E D Morrison, C D Stiles
{"title":"The beta-interferon-mediated antimitogenic state resembles the anti-viral state.","authors":"M A Garcia-Blanco,&nbsp;J A Porter,&nbsp;E D Morrison,&nbsp;C D Stiles","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Much controversy regarding the effect of interferons in control of the animal cell cycle can be reconciled by acknowledging that, until recently, few laboratories enjoyed access to both pure interferons and pure growth-factor preparations. Using such reagents, we show data that suggest that antimitogenic state in Balb/c-3T3 cells by beta-interferon resembles the antiviral state in two important regards: dosage and kinetics. Picomolar concentrations of homogeneous beta-interferon inhibit the mitogenic response to recombinant platelet-derived growth-factor B chain homodimer. The antimitogenic response is seen only when cells are exposed to pure beta-interferon for an extended time prior to platelet-derived growth-factor treatment. The interferon-mediated antimitogenic state is exerted at some point in the cell cycle subsequent to c-myc gene induction.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"323-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13356832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human ltk: gene structure and preferential expression in human leukemic cells. 人类ltk基因结构及其在人类白血病细胞中的优先表达。
Oncogene research Pub Date : 1990-01-01
Y Maru, H Hirai, F Takaku
{"title":"Human ltk: gene structure and preferential expression in human leukemic cells.","authors":"Y Maru,&nbsp;H Hirai,&nbsp;F Takaku","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have isolated the human homolog (hltk) of the murine tyrosine kinase gene ltk from a K-562 human leukemia cDNA library. The deduced protein sequence of hltk is 17 amino acids longer in the juxtamembrane domain and 28 amino acids shorter in the carboxy terminus than that of murine ltk. The partially identical splicing points of hltk to those of c-ros showed a close genetic linkage between the two. In Northern blot analysis of 35 human malignancies, hltk is preferentially expressed in leukemias (10 out of 18 cases) with no cell lineage specificity, but none of 17 nonleukemic neoplasms expressed hltk gene.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 3","pages":"199-204"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13469482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3;5)(q25;q34) translocations. 人类Evi-1基因位于染色体3q24-q28上，但在3例含有t(3;5)(q25;q34)易位的急性非淋巴细胞白血病中没有重排。
Oncogene research Pub Date : 1990-01-01
K Morishita, E Parganas, C Bartholomew, N Sacchi, M B Valentine, S C Raimondi, M M Le Beau, J N Ihle
{"title":"The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3;5)(q25;q34) translocations.","authors":"K Morishita,&nbsp;E Parganas,&nbsp;C Bartholomew,&nbsp;N Sacchi,&nbsp;M B Valentine,&nbsp;S C Raimondi,&nbsp;M M Le Beau,&nbsp;J N Ihle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The murine Evi-1 gene encodes a protein that has multiple 28-amino acid repeats containing the consensus sequence found in the zinc finger domains of many transcriptional regulatory proteins. Activation of the expression of the Evi-1 gene is frequently found in murine myeloid leukemias and leukemia cell lines and is due to retroviral insertions in the 5' region of the gene in either the Evi-1 or the CB-1/FIM3 common sites of viral integrations. To examine the role of the Evi-1 gene in human leukemias we have cloned regions of the human locus corresponding to the coding region of the gene and regions corresponding to the Evi-1 and CB-1/FIM3 common sites of integrations. Using these probes we demonstrate that the human Evi-1 gene maps to chromosome 3q24-q28 in a region that is translocated in acute nonlymphocytic leukemias with a t(3;5)(q25;q34). By in situ hybridization with metaphase chromosomes from one patient with a 3;5 translocation, the Evi-1 gene was found to be translocated to the derivative 5 chromosome. However, no rearrangements were detected by Southern blot analysis with DNAs from three patients with a t(3;5) using probes from the Evi-1 or CB-1/FIM3 loci. No Evi-1 transcripts were detected with RNA from leukemic blasts of one patient with a t(3;5).</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 3","pages":"221-31"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13263874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An E. coli expression system for the rapid purification and characterization of a v-abl tyrosine protein kinase. 一种快速纯化和鉴定v-abl酪氨酸蛋白激酶的大肠杆菌表达系统。
Oncogene research Pub Date : 1990-01-01
N B Lydon, B Adams, J F Poschet, A Gutzwiller, A Matter
{"title":"An E. coli expression system for the rapid purification and characterization of a v-abl tyrosine protein kinase.","authors":"N B Lydon,&nbsp;B Adams,&nbsp;J F Poschet,&nbsp;A Gutzwiller,&nbsp;A Matter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A bacterial expression vector containing a segment of the v-abl gene from Abelson murine leukemia virus (A-MuLV) was constructed such that the gag region of v-abl was replaced by a sequence encoding the IgG-binding domain of the S. aureus protein A. pabl HP, a fusion protein encoded by this vector was rapidly purified to near homogeneity by affinity chromatography on IgG-Affigel and Mono Q FPLC. The Km of the pabl HP kinase for ATP varied with [Val5]-angiotensin II concentration and was 21.2 microM at saturating concentrations of [Val5]-angiotensin II. The Km for [Val5]-angiotensin II at saturating concentrations of ATP was 3.8 mM. The turnover number, at 20 degrees C, was 62 mumol min-1 mumol-1. Initial rate studies support a ternary complex kinetic mechanism for phosphoryl transferase. The substrate specificity of the pabl HP kinase was further characterized using synthetic peptides. This expression system, which enables the rapid purification of recombinant v-abl kinase is suitable for the comparative enzymological study of mutant v-abl enzymes generated by site-directed mutagenesis.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 3","pages":"161-73"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13310481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence that a phosphotyrosine-containing 120,000 Da protein from Rous sarcoma virus-infected cells is phosphorylated by pp60v-src. 来自劳斯肉瘤病毒感染细胞的含有120000 Da的磷酸酪氨酸蛋白被pp60v-src磷酸化的证据
Oncogene research Pub Date : 1989-01-01
A F Lau
{"title":"Evidence that a phosphotyrosine-containing 120,000 Da protein from Rous sarcoma virus-infected cells is phosphorylated by pp60v-src.","authors":"A F Lau","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Phosphorylation of a phosphotyrosine-containing 120,000 Da protein (pp120) in Rous sarcoma virus (RSV)-infected mammalian cells and in in vitro protein kinase reactions was examined. Phosphorylated pp120 was co-immunoprecipitated with anti-pp60v-src antibodies only from RSV-transformed or revertant vole fibroblasts which contained active pp60v-src tyrosine kinase activity or from temperature-sensitive RSV-infected vole cells grown at the permissive temperature. Pp120 was phosphorylated on tyrosine in in vitro immune complex kinase reactions containing both pp120 and enzymatically active pp60v-src. Inhibition of pp60v-src's kinase activity blocked phosphorylation of pp120 in vitro. These results support the proposal that pp120 tyrosine phosphorylation is pp60v-src-dependent and that pp120 may thus serve as a substrate of pp60v-src in RSV-transformed mammalian cells.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"4 3","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13619641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Localization of cellular src mRNA during development and in the differentiated bipolar neurons of the adult neural retina in Xiphophorus. 剑鱼发育过程中细胞src mRNA的定位及在成年神经视网膜分化双极神经元中的定位。
Oncogene research Pub Date : 1989-01-01
F Raulf, W Mäueler, S M Robertson, M Schartl
{"title":"Localization of cellular src mRNA during development and in the differentiated bipolar neurons of the adult neural retina in Xiphophorus.","authors":"F Raulf,&nbsp;W Mäueler,&nbsp;S M Robertson,&nbsp;M Schartl","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The expression of the c-src gene in embryonic and adult tissue of the teleost fish Xiphophorus helleri was analyzed by in-situ hybridization. The highly conserved fish c-src gene was found to be expressed at high levels in midterm embryos, where c-src mRNA was localized in developing neurons of the sensory layer of the differentiating retina and in the developing brain. In adult tissues the expression of c-src was found to persist in certain cell types of the brain and the neural retina, especially in the bipolar cells of the inner nuclear layer, which are postmitotic, fully differentiated mature neurons. Thus c-src in Xiphophorus appears to be a developmentally regulated proto-oncogene which is important for neuronal differentiation during organogenesis, but whose persistence of expression in certain terminally differentiated neurons strongly suggests a particular maintenance function for c-src in these cells as well.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 1","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13918567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence that stimulation of growth following long term density arrest of WI-38 cells proceeds via a pathway independent of protein kinase C and of cAMP-dependent protein kinase. 有证据表明，WI-38细胞长期密度阻滞后的生长刺激是通过独立于蛋白激酶C和camp依赖性蛋白激酶的途径进行的。
Oncogene research Pub Date : 1989-01-01
T A Owen, S C Cosenza, D R Soprano, K J Soprano
{"title":"Evidence that stimulation of growth following long term density arrest of WI-38 cells proceeds via a pathway independent of protein kinase C and of cAMP-dependent protein kinase.","authors":"T A Owen,&nbsp;S C Cosenza,&nbsp;D R Soprano,&nbsp;K J Soprano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When cultures of WI-38 human diploid fibroblasts reach high cell densities, they cease to proliferate and enter a viable state of quiescence in which they can remain for long periods of time. However, the longer the cells remain growth arrested, the more time they require to leave G-0, to progress through G-1, and to enter S following stimulation with fresh serum. Previous results from our laboratory showed that in this system the time of expression of c-fos and c-myc was related only to the time of stimulation and not to the time of DNA synthesis [Owen et al., (1985) J. Biol. Chem. 262, 15111-15117]. It is possible that the initial response of both the short and long term quiescent WI-38 cells to growth factor stimulation was the same but that the subsequent secondary events which led to the progression of the stimulated cells through G-1 into S were different. Therefore, experiments were performed to compare the signal transduction pathway(s) utilized following serum stimulation of long term quiescent WI-38 cells to those utilized in WI-38 cells stimulated after short periods of time in quiescence. The only differences detected between the short and long term quiescent WI-38 cells involved their responses to epidermal growth factor (EGF). These results suggest that the response of quiescent WI-38 cells to fetal calf serum involves an EGF-dependent signal transduction pathway. Moreover, the EGF pathway which functions in long term quiescent WI-38 cells would appear to be defective compared to that employed by short term quiescent WI-38 cells.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"4 2","pages":"137-47"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13642335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nucleotide sequence and structural organization of the human FMS proto-oncogene. 人FMS原癌基因的核苷酸序列和结构组织。
Oncogene research Pub Date : 1989-01-01
A Hampe, B M Shamoon, M Gobet, C J Sherr, F Galibert
{"title":"Nucleotide sequence and structural organization of the human FMS proto-oncogene.","authors":"A Hampe,&nbsp;B M Shamoon,&nbsp;M Gobet,&nbsp;C J Sherr,&nbsp;F Galibert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The human proto-oncogene c-fms [FMS] on chromosome 5q33.3 encodes a transmembrane glycoprotein with tyrosine kinase activity that functions as the cell surface receptor for the macrophage colony stimulating factor (CSF-1 or M-CSF). Overlapping bacteriophage clones that included 35 kb of the FMS locus and contained the complete coding sequence of the CSF-1 receptor were subjected to nucleotide sequencing analysis. Comparison with the cDNA sequence of the human c-fms gene indicated that at least one 5' noncoding exon is located far upstream (ca. 26 kb) from sequences encoding the CSF-1 receptor. The FMS coding sequence consists of 21 small exons and heterogeneously sized introns, ranging from 6.3 kb to less than 0.1 kb in complexity.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"4 1","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13666802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of alternate and truncated forms of murine c-myb proteins. 小鼠c-myb蛋白的交替和截断形式的表征。
Oncogene research Pub Date : 1989-01-01
R G Ramsay, S Ishii, Y Nishina, G Soe, T J Gonda
{"title":"Characterization of alternate and truncated forms of murine c-myb proteins.","authors":"R G Ramsay,&nbsp;S Ishii,&nbsp;Y Nishina,&nbsp;G Soe,&nbsp;T J Gonda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The expression of c-myb proteins in transformed and normal murine cells was investigated. Two c-myb proteins, p75c-myb and p90c-myb, were detected in normal thymocytes and cell lines with intact c-myb genes. These most likely differ by the inclusion of additional amino acids encoded by an alternatively spliced c-myb mRNA. The use of this alternative exon is therefore not a feature exclusively of those cells with viral integrations in their c-myb gene. Smaller c-myb proteins in myeloid leukemic cell lines with rearranged c-myb genes were also characterized. Viral integration into the 5' region of the c-myb gene in the W265 and W274 cell lines leads to the synthesis in each case of two amino-terminally truncated c-myb proteins. By contrast, in NFS60 cells, viral integration into a more 3' region of the c-myb locus (but upstream of an alternate exon) leads to the production of a single p50c-myb protein.</p>","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"4 4","pages":"259-69"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13692917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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