Oncogene research最新文献

Interleukin-3 and phorbol esters induce different patterns of immediate-early gene expression in an interleukin-3 dependent cell line. 白细胞介素-3和佛波酯在白细胞介素-3依赖的细胞系中诱导不同的早期基因表达模式。

Oncogene research Pub Date : 1991-01-01

J A McCubrey, L S Steelman, J P McKearn

{"title":"Interleukin-3 and phorbol esters induce different patterns of immediate-early gene expression in an interleukin-3 dependent cell line.","authors":"J A McCubrey, L S Steelman, J P McKearn","doi":"","DOIUrl":"","url":null,"abstract":"The myeloid interleukin-3 (IL-3) dependent cell line, FDC-P1, enters the G0 stage of the cell cycle after IL-3 deprivation for 24 hr. The expression of 13 protooncogenes and immediate-early genes was compared with 4 \"control\" genes after the addition of either IL-3 or phorbol myristate acetate (PMA) to IL-3-deprived cells. mRNA transcripts encoding c-myc and the T-cell receptor c-gamma gene were induced to high levels only after IL-3 addition, whereas c-fos, fos-B, c-jun, jun-B, Krox-20, and Krox-24 were induced transiently only after PMA addition. The remaining genes (fra-1, p53, jun-D, c-Ha-ras, c-Ki-ras, c-raf, beta-actin, ornithine decarboxylase, and histone 2B) were detected after culture with either IL-3 or PMA. When cells were serum- and IL-3-deprived, c-fos, fos-B, c-jun, jun-B, Krox-20, and Krox-24 were detected after exposure to either serum or PMA. Moreover, culture with cycloheximide and PMA resulted in superinduction of these genes, whereas cycloheximide and IL-3 addition did not. mRNAs encoding these genes had half-lives of 10-20 min after PMA treatment, whereas that of beta-actin was longer (greater than 2 hr), suggesting that short mRNA half-lives contributed to the transient nature of these genes. Although c-fos, fos-B, c-jun, jun-B, Krox-20, and Krox-24 expression can be detected in IL-3-dependent cells after exposure to either PMA or serum, these genes were not detected after IL-3 addition, which allows cell-cycle progression. These results document the existence of IL-3 and PMA-responsive genes and demonstrate that IL-3 and protein kinase C agonists can induce distinct patterns of gene expression.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"6 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of mutations of oncogene C-Ha-ras at codon 12 with metastasis and survival of gastric cancer patients. 癌基因C-Ha-ras密码子12突变与胃癌患者转移及生存的相关性

Oncogene research Pub Date : 1991-01-01

G R Deng, X H Liu, J R Wang

引用次数: 0

Binding of a nuclear factor to the upstream region of the c-myc gene. 核因子与c-myc基因上游区域的结合。

Oncogene research Pub Date : 1991-01-01

K Saksela, P J Koskinen, K Alitalo

引用次数: 0

Introduction of an activated RAS oncogene into murine bone marrow lymphoid progenitors via retroviral gene transfer results in thymic lymphomas. 通过逆转录病毒基因转移将激活的RAS癌基因导入小鼠骨髓淋巴样祖细胞可导致胸腺淋巴瘤。

Oncogene research Pub Date : 1991-01-01

C E Dunbar, P S Crosier, A W Nienhuis

{"title":"Introduction of an activated RAS oncogene into murine bone marrow lymphoid progenitors via retroviral gene transfer results in thymic lymphomas.","authors":"C E Dunbar, P S Crosier, A W Nienhuis","doi":"","DOIUrl":"","url":null,"abstract":"Activating mutations within the various RAS protooncogenes have been detected in many human and murine hematopoietic neoplasms. Their causal significance has been difficult to assess, partly due to the lack of an animal model directly linking these mutations to hematopoietic neoplasms. A high-titer, helper-free recombinant retrovirus was used to introduce an activated Harvey RAS gene under the transcriptional control of the Moloney leukemia virus LTR into murine bone marrow cells. Eleven of fifteen mice reconstituted with these bone marrow cells developed fatal thymic lymphomas 10-12 week post-transplant. Analysis of DNA and RNA from tumor cells revealed the integrated proviral genome and provirally encoded RAS mRNA respectively. Immunophenotyping and T-cell receptor rearrangement analysis of fresh tumor cells and of cell lines derived from these tumors showed them to be T-cells arrested midway through thymic development. Despite evidence of proviral integration in marrow cells of mice with thymic tumors, no other hematologic abnormalities were detected. The short latency and reproducibility of thymic lymphoma development in mice transplanted with marrow transduced with this retrovirus suggests a direct causal effect of expression of an activated RAS gene in the transformation of a bone-marrow-derived lymphoid progenitor.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"6 1","pages":"39-51"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Int-5, a locus associated with early events in mammary carcinogenesis. 与乳腺癌早期事件相关的基因座Int-5的特征。

Oncogene research Pub Date : 1991-01-01

V L Morris, T R Rao, C A Kozak, D A Gray, E C Lee Chan, T J Cornell, C B Taylor, R F Jones, C M McGrath

{"title":"Characterization of Int-5, a locus associated with early events in mammary carcinogenesis.","authors":"V L Morris, T R Rao, C A Kozak, D A Gray, E C Lee Chan, T J Cornell, C B Taylor, R F Jones, C M McGrath","doi":"","DOIUrl":"","url":null,"abstract":"Three chemically-induced precancerous mammary hyperplasias, independently isolated in BALB/c mice, all contained mouse mammary tumor virus (MMTV) proviral DNA integrated into a common region in chromosomal DNA, designated Int-5 (Formerly Int-H, Gray et al., 1986). This site was cloned from a hyperplastic outgrowth (D2) into lambda phage. A 1.7 Kb Hind III DNA fragment, which flanks the 5' end of the MMTV insert, was generated from the cloned Int-5 region. This fragment was used as probe (IH-2) to localize Int-5 to mouse chromosome 9. The IH-2 sequence was highly conserved in DNA of several mammalian species including man, in three other widely divergent vertebrate phyla, and in C. elegans. The Int-5 region, containing 5.6 Kb 5' and 12.8 Kb 3' to the MMTV integration site in D2, was cloned in EMBL-3 from a BALB/c genomic DNA library. cDNA complementary to poly A+ lactating mammary gland RNA, annealed with Sst-1 fragments spanning most of the BALB/c Int-5 clone. The highest level of Int-5 specific poly A+ mRNA was detected in D2 tumor. Lactating mammary gland and D2 hyperplastic alveolar nodule contained 5-fold less Int-5 RNA while liver contained 8-fold less Int-5 RNA. Int-5 cDNA (IH-3) annealed with two RNA species of approximately 3.3 and 4.0 Kb. These data are consistent with the hypothesis that Int-5 contains an oncogene, different from any other previously described, involved in early events in some models of chemical carcinogenesis.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"6 1","pages":"53-63"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformation of rat bladder epithelial cells by introduction of a single oncogene. 引入单一致癌基因对大鼠膀胱上皮细胞的转化作用。

Oncogene research Pub Date : 1991-01-01

A M Mann, M Stevenson, T Masui, C D Borgeson, S M Cohen

引用次数: 0

Walking along human c-myc mRNA with antisense oligodeoxynucleotides: maximum efficacy at the 5' cap region. 用反义寡脱氧核苷酸沿着人c-myc mRNA行走:在5'帽区域的最大功效。

Oncogene research Pub Date : 1991-01-01

T A Bacon, E Wickstrom

引用次数: 0

Daily addition of an anti-c-myc DNA oligomer induces granulocytic differentiation of human promyelocytic leukemia HL-60 cells in both serum-containing and serum-free media. 每日添加抗c-myc DNA寡聚物诱导人早幼粒细胞白血病HL-60细胞在含血清和无血清培养基中向粒细胞分化。

Oncogene research Pub Date : 1991-01-01

T A Bacon, E Wickstrom

引用次数: 0

Tyrosine protein kinase activity of the HZ4-feline sarcoma virus P80gag-kit-transforming protein. hz4 -猫肉瘤病毒p80gag -kit转化蛋白酪氨酸蛋白激酶活性的研究

Oncogene research Pub Date : 1990-01-01

S Majumder, P Ray, P Besmer

引用次数: 0

The murine c-rel proto-oncogene encodes two mRNAs the expression of which is modulated by lymphoid stimuli. 小鼠c-rel原癌基因编码两种mrna，其表达受淋巴细胞刺激调节。

Oncogene research Pub Date : 1990-01-01

R J Grumont, S Gerondakis

{"title":"The murine c-rel proto-oncogene encodes two mRNAs the expression of which is modulated by lymphoid stimuli.","authors":"R J Grumont, S Gerondakis","doi":"","DOIUrl":"","url":null,"abstract":"Here we report a survey of c-rel proto-oncogene transcription in murine tissues, cell lines and lymphoid cells. In addition to the previously described 7.5-kb mRNA, we have identified a mRNA of 2.5-kb. As DNA hybridization indicates that there is only one gene with significant homology to c-rel in the mouse genome, it appears that multiple mRNAs are transcribed from c-rel. The nucleotide sequence of a cDNA clone derived from the 2.5-kb c-rel mRNA demonstrates that the 7.5- and 2.5-kb mRNAs encode identical proteins. The different size of the two mRNAs is due to variation in the length of the 3' untranslated region, which arises from the use of alternate polyadenylation signals. These mRNAs are present at low levels in organs tested, and in cell lines representing a wide variety of lineages. Fibroblasts are the only cells in which expression was not detectable. In B-cell lines representing different stages of differentiation, the highest levels of mRNA are seen in B-lymphomas, and this level drops markedly in plasmacytomas. There is a transient increase of 10- to 20-fold in the level of c-rel mRNAs in T-cells treated with concanavalin A, while lipopolysaccharide-stimulated B-cells exhibit a transient 5-fold elevation of c-rel expression. This study indicates that the control of c-rel expression can vary between and within different cell lineages, and the widespread expression of this gene points to a fundamental cellular function, rather than one restricted to hematopoietic cells as previously suggested.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"245-54"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13356211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0