Oncogene research最新文献_第2页

Selective potentiation of c-fps/fes transforming activity by a phosphatase inhibitor. 磷酸酶抑制剂选择性增强c-fps/fes转化活性。

Oncogene research Pub Date : 1990-01-01

R A Feldman, D R Lowy, W C Vass

{"title":"Selective potentiation of c-fps/fes transforming activity by a phosphatase inhibitor.","authors":"R A Feldman, D R Lowy, W C Vass","doi":"","DOIUrl":"","url":null,"abstract":"Human c-fps/fes when expressed at sufficiently high levels in NIH 3T3 cells can induce cellular transformation. To probe the mechanism of action of the c-fps/fes product NCP92, we have examined the biological and biochemical consequences of stabilizing phosphotyrosine in cells that overexpress NCP92. In this study we report that when cells expressing c-fps/fes are incubated with low concentrations of sodium vanadate, a tyrosine phosphatase inhibitor, the transforming activity of c-fps/fes can be potentiated by nearly two orders of magnitude. This effect was associated with a threefold increase in the level of phosphotyrosine in NCP92 and its major cellular substrates. Unlike c-src, NCP92 had a single tyrosine phosphorylation site, and vanadate treatment induced its phosphorylation in vivo. This was found to have a positive effect on NCP92 kinase specific activity, but at the low concentrations of vanadate that were used, this effect was very small. The results are consistent with the hypothesis that potentiation was a consequence of the stabilization of phosphotyrosine in critical targets of transformation of NCP92, rather than from a direct effect of vanadate on NCP92 kinase. The potentiating effect of vanadate was relatively specific for c-fps/fes; this reagent did not affect the high transforming activity of gag-v-fps/fes, nor did it enhance the less active c-ras, c-src, or EGF receptor genes, although the latter two genes encode tyrosine kinases. The specificity of the biological response of c-fps/fes to the stabilization of phosphotyrosine suggests that this molecule has a distinct mode of regulation and mechanism of action.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 3","pages":"187-97"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13310483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target dependence of antisense oligodeoxynucleotide inhibition of c-Ha-ras p21 expression and focus formation in T24-transformed NIH3T3 cells. 反义寡脱氧核苷酸抑制t24转化NIH3T3细胞c-Ha-ras p21表达和病灶形成的靶标依赖性

Oncogene research Pub Date : 1990-01-01

Y Daaka, E Wickstrom

引用次数: 0

Oncogene expression and immunoglobulin synthesis in a North American Burkitt (NAB-2) lymphoma cell line with a 8;22 chromosome translocation. 8;22染色体易位的北美伯基特(NAB-2)淋巴瘤细胞系的癌基因表达和免疫球蛋白合成

Oncogene research Pub Date : 1990-01-01

N C Popescu, J E Dahlberg, D V Ablashi, M Monastier, C A Bona, J A DiPaolo, W C Hooper, D C Swan

{"title":"Oncogene expression and immunoglobulin synthesis in a North American Burkitt (NAB-2) lymphoma cell line with a 8;22 chromosome translocation.","authors":"N C Popescu, J E Dahlberg, D V Ablashi, M Monastier, C A Bona, J A DiPaolo, W C Hooper, D C Swan","doi":"","DOIUrl":"","url":null,"abstract":"A Burkitt's lymphoma (BL) cell line, (NAB-2) deriving from a North American patient, exhibited a 8;22 (q22;q11-12) translocation involving the c-myc gene and lambda immunoglobulin genes. In addition, NAB-2 cells have two other consistent translocations: a 1;5 (p22;q23) and a 3;7 (p25;q22), with breakpoints close to the location of N-ras, fms, and raf-1 protooncogenes, respectively. In situ hybridization with myc, N-ras, and raf-1 radiolabeled DNA probes to NAB-2 chromosomes showed that none of these genes was relocated as a result of translocation. However, by Northern blot analysis, the myc mRNA was represented by two transcripts, one approximately 2.4 kb and the other considerably larger (74 kb). The raf-1 gene transcript was also detected in NAB-2 cells; however, its size and level were similar to those seen in two other BL lines. On the other hand, the N-ras, fms, and fgr genes, which are frequently activated in BL, were not actively transcribed. NAB-2 cells also have a chromatid defect visible as an achromatic region on the short arm of chromosome 2 near the locus of the kappa immunoglobulin gene. This alteration, which is a viral modification site caused by the Epstein-Barr virus or viral products, had no influence on immunoglobulin synthesis, as NAB-2 cells concordant to the 8;22 translocation were positive for cytoplasmic and surface lambda light chains. Although NAB-2 cells exhibit several chromosomal abnormalities, only translocation, 8;22 was associated with gene alterations relevant to the neoplastic development of this malignancy.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"295-303"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12862306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of avian retroviruses carrying activated transforming human c-src genes and of steps involved in expression of activated src-PKases in vitro. 携带活化的转化人c-src基因的禽逆转录病毒的特性和活化的src- pkase在体外表达的步骤。

Oncogene research Pub Date : 1990-01-01

A Tanaka, M Salem, R J Eckroade, D J Fujita

{"title":"Characterization of avian retroviruses carrying activated transforming human c-src genes and of steps involved in expression of activated src-PKases in vitro.","authors":"A Tanaka, M Salem, R J Eckroade, D J Fujita","doi":"","DOIUrl":"","url":null,"abstract":"We have isolated four activated transforming human c-src mutants derived spontaneously from viruses carrying the normal human c-src (SRC) genes in the form of Rous sarcoma virus. These mutants induced transformed cell morphology distinguishable from each other in vitro as well as tumors in chicks, whereas normal SRC-carrying viruses did not. Analyses of the transforming SRC proteins together with the normal SRC protein showed that levels of the carboxy-terminal Tyr-phosphorylation were negatively correlated with both transforming ability and protein kinase (PKase) activity as determined by in vitro autophosphorylation. It was observed that two cell lysis methods, that is, NP-40 and RIPA, yielded two different phosphorylated forms of transforming SRC proteins: one possessed low levels of phosphorylation at the autophosphorylation site and the other possessed high levels of phosphorylation at this site. Using the two types of transforming SRC preparations, we have studied in vitro SRC-PKase reactions in relation to in vivo and in vitro autophosphorylation and in vitro phosphorylation of an exogenous substrate. A possible functional relationship between autophosphorylation and SRC-PKase expression is discussed.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"305-22"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13322045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, sequencing, and expression of mouse c-ets-1 cDNA in baculovirus expression system. 杆状病毒表达系统中小鼠c-ets-1 cDNA的克隆、测序和表达。

Oncogene research Pub Date : 1990-01-01

J H Chen

{"title":"Cloning, sequencing, and expression of mouse c-ets-1 cDNA in baculovirus expression system.","authors":"J H Chen","doi":"","DOIUrl":"","url":null,"abstract":"The protooncogene c-ets-1 is preferentially expressed in lymphoid cells. The protein product of this gene has been found to be a phosphorylated nuclear protein. When lymphocytes are stimulated with calcium ionophore, hyperphosphorylation of c-ets-1 occurs. In order to study the biological and biochemical functions of the c-ets-1 protein in detail, it is important to prepare adequate quantity of the c-ets-1 protein. To this end, we have cloned, sequenced, and expressed mouse c-ets-1 cDNA in baculovirus expression vector. Sequence analysis indicated that mouse c-ets-1 cDNA codes for a 50/51-kd protein. Since the mouse c-ets-1 protein in mouse lymphocytes is a 60/62-kd protein, the result obtained indicated that the c-ets-1 protein undergoes posttranslational modification by phosphorylation. When the c-ets-1 cDNA was expressed in the baculovirus expression vector, insect cells infected with a recombinant virus synthesizes a protein of the same size but with 50-100 times more of the c-ets-1 protein than that of mouse lymphocytes. The Staphylococcus aureus V8 protease mapping analysis of mouse c-ets-1 proteins synthesized in mouse and insect cells showed that they are identical. Thus, the c-ets-1 protein synthesized in insect cells will allow us to purify and study the functions of the c-ets-1 protein in detail.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"277-85"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13356830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of renal and hepatic c-myc and c-Ha-ras expression by unilateral nephrectomy. 单侧肾切除术对肾脏和肝脏c-myc和c-Ha-ras表达的刺激。

Oncogene research Pub Date : 1990-01-01

A Bailey, J D Sanchez, D Rigsby, J Roesel, R Alvarez, B Rodu, D M Miller

{"title":"Stimulation of renal and hepatic c-myc and c-Ha-ras expression by unilateral nephrectomy.","authors":"A Bailey, J D Sanchez, D Rigsby, J Roesel, R Alvarez, B Rodu, D M Miller","doi":"","DOIUrl":"","url":null,"abstract":"Unilateral nephrectomy induces compensatory hypertrophy of the contralateral kidney in rats, resulting in a 25% weight increase in 14 days. We have demonstrated that expression of the c-myc and c-Ha-ras protooncogenes is increased more than ten-fold in the contralateral kidney within 4 to 8 hr following unilateral nephrectomy in rats. The increased expression of these genes is analogous to the increased expression of c-myc and c-Ha-ras that occurs early in liver regeneration, preceding the first increase in DNA synthesis by at least 20 hr. In order to define the tissue specificity of the signals for compensatory renal hypertrophy, we also determined the early protooncogene response and the proliferative response in the liver of rats following unilateral nephrectomy. The expression of c-myc and c-Ha-ras was also increased (five- to ten-fold) in the livers of these animals. DNA synthesis was stimulated in the contralateral kidney at 26-30 hr following nephrectomy as measured by 3H thymidine incorporation, indicating a hyperplastic response to unilateral nephrectomy. However, there was no increase in DNA synthesis in the liver despite the dramatic increase in c-myc and c-Ha-ras expression. Our data suggest that the early increase in protooncogene expression in response to unilateral nephrectomy is stimulated by circulating signals that are not tissue-specific. Increased protooncogene expression in both kidney and liver following unilateral nephrectomy is an early response to the regenerative stimulus, but later signals must provide the tissue specificity necessary for regeneration and cellular proliferation.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 4","pages":"287-93"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13356831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retinoic acid causes a decline in TGF-alpha expression, cloning efficiency, and tumorigenicity in a human embryonal cancer cell line. 维甲酸在人胚胎癌细胞系中引起tgf - α表达、克隆效率和致瘤性的下降。

Oncogene research Pub Date : 1990-01-01

E Dmitrovsky, D Moy, W H Miller, A Li, H Masui

{"title":"Retinoic acid causes a decline in TGF-alpha expression, cloning efficiency, and tumorigenicity in a human embryonal cancer cell line.","authors":"E Dmitrovsky, D Moy, W H Miller, A Li, H Masui","doi":"","DOIUrl":"","url":null,"abstract":"The human teratocarcinoma NTERA-2 cl. D1 (NT2/D1) cell is a cloned multipotential embryonal cancer cell line that differentiates into a neuronal phenotype and other cellular lineages with retinoic acid (RA) treatment. Here we report that mRNA for the transforming growth factor-alpha is expressed in these RA-untreated cells and that RA-treatment results in a reduction of mRNA expression within 24 hr of treatment. In total cellular RNA, TGF-alpha mRNA is not detectable by Northern analysis at 6 days when there is increased expression of the human homeotic genes Hu-1 (Hox 2.1) and Hu-2 (Hox 2.2), known markers of RA response in NT2/D1 cells. RA treatment also causes a marked reduction in cloning efficiency and tumorigenicity of these cells. The addition of TGF-alpha or EGF (epidermal growth factor) protein to RA-untreated NT2/D1 cells augments soft agar cloning under limited fetal calf serum conditions. Blocking monoclonal antibodies directed against the EGF receptor (EGFr) can prevent this augmentation. We conclude that TGF-alpha expression inversely correlates with the state of RA-induced differentiation of this human teratocarcinoma cell and that TGF-alpha and EGF proteins are stimulatory growth factors in NT2/D1 cells under these culture conditions.","PeriodicalId":77583,"journal":{"name":"Oncogene research","volume":"5 3","pages":"233-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13469483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human basic fibroblast growth factor gene is located on the long arm of chromosome 4 at bands q26-q27. 人碱性成纤维细胞生长因子基因位于4号染色体长臂q26-q27带。

Oncogene research Pub Date : 1990-01-01

M Lafage-Pochitaloff, F Galland, J Simonetti, H Prats, M G Mattei, D Birnbaum

引用次数: 0

Analysis of mutant forms of the c-src gene product containing a phenylalanine substitution for tyrosine 416. 含有苯丙氨酸替代酪氨酸416的c-src基因产物的突变形式分析。

Oncogene research Pub Date : 1990-01-01

R Ferracini, J Brugge

引用次数: 0

Isolation and characterization of flat revertant cell lines from A-MuLV-transformed fibroblasts. 从a - mulv转化成纤维细胞中分离和鉴定扁平逆转细胞系。

Oncogene research Pub Date : 1990-01-01

D J Glass, R W Rees-Jones, S P Goff

引用次数: 0