Biomaterials, artificial cells, and artificial organs最新文献_第4页

Development of a DNA-adsorbent for the specific removal of anti-DNA autoantibodies in systemic lupus erythematosus (SLE). 用于系统性红斑狼疮(SLE)特异性去除抗dna自身抗体的dna吸附剂的研制。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117337

F Hiepe, K Wolbart, W Schossler, A Speer, T Montag, F Mielke, E Apostoloff

{"title":"Development of a DNA-adsorbent for the specific removal of anti-DNA autoantibodies in systemic lupus erythematosus (SLE).","authors":"F Hiepe, K Wolbart, W Schossler, A Speer, T Montag, F Mielke, E Apostoloff","doi":"10.3109/10731199009117337","DOIUrl":"https://doi.org/10.3109/10731199009117337","url":null,"abstract":"Autoantibodies against DNA are of primary importance for the diagnosis and pathogenesis of systemic lupus erythematousus (SLE). The level of anti-DNA antibodies correlates well with the disease activity and renal involvement. In such patients the removal of anti-DNA antibodies from plasma may lead to a clinical improvement. For this reason an adsorbent was made by covalent coupling of calf thymus DNA to a solid support based on ethylene dimethacrylate cross-linked hydroxethyl methacrylate. Up to 2.5 mg of DNA were immobolized to 1 ml of the support activated chemically by aminosilane and glutaraldehyde. The incubation of 40 ml of SLE plasma with 1 ml of the adsorbent resulted in a 50% decline in anti-DNA activity. There was no release of immobilized P-32-DNA into the plasma. Biocompatibility, sterilisation, and reapplication (without loss of binding capacity) of the adsorbent could be demonstrated. We concluded that the adsorbent may be suitable for treatment.","PeriodicalId":77498,"journal":{"name":"Biomaterials, artificial cells, and artificial organs","volume":"18 5","pages":"683-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199009117337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13254718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Artificial red cells. A link between the membrane skeleton and RES detectability? 人造红细胞。膜骨架与RES可探测性之间的联系?

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117311

R D MacGregor, C A Hunt

{"title":"Artificial red cells. A link between the membrane skeleton and RES detectability?","authors":"R D MacGregor, C A Hunt","doi":"10.3109/10731199009117311","DOIUrl":"https://doi.org/10.3109/10731199009117311","url":null,"abstract":"Factors governing nonspecific reticuloendothelial system (RES)-detectability are largely unknown. Will a liposome that mimics the lipid composition of the outer leaflet of the erythrocyte membrane be invisible to the RES? On both experimental and theoretical grounds we believe the answer is no, in part because 1) sorption of proteins is believed to be important in determining RES uptake, 2) a membrane skeleton is apparently necessary to inhibit protein sorption into erythrocyte membranes and 3) Neohemocytes (a liposome encapsulated hemoglobin product) currently lack a membrane skeleton. Neohemocytes with erythrocyte outer leaflet lipid composition do have extended circulation half-times, but these are at least two orders of magnitude shorter than the circulation half-times of erythrocytes. How might a membrane skeleton modulate RES-detectability? Can avoidance of opsonization result in part from the properties of the membrane skeleton? If so, then how? To explore and quantify such questions we have developed a theoretical, statistical-thermodynamic model of protein binding into membranes. It predicts that the membrane area available for rapid lateral diffusion is critically important in controlling the amount of sorbed protein per unit area, and that a membrane skeleton can reduce a protein's sorption by several orders of magnitude. Based on theoretical results, we offer a speculative model for the detection of non-self lipid bilayers by the RES.","PeriodicalId":77498,"journal":{"name":"Biomaterials, artificial cells, and artificial organs","volume":"18 2","pages":"329-43"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199009117311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13519428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Whole complement hemolytic activity (CH50) following infusion of stroma-free and polyhemoglobin solutions in rats. 大鼠灌注无基质和多血红蛋白溶液后的全补体溶血活性(CH50)。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117302

J Ning, T M Chang

引用次数: 4

Coronary vasoconstrictor activity of purified and modified human hemoglobin. 纯化和修饰的人血红蛋白的冠状动脉收缩活性。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117306

V W Macdonald, R M Winslow, M A Marini, M T Klinker

{"title":"Coronary vasoconstrictor activity of purified and modified human hemoglobin.","authors":"V W Macdonald, R M Winslow, M A Marini, M T Klinker","doi":"10.3109/10731199009117306","DOIUrl":"https://doi.org/10.3109/10731199009117306","url":null,"abstract":"Unpurified stroma-free hemoglobin (SFH) from water-lysed human red blood cells, hemoglobin Ao (HbAo), and hemoglobin cross-linked between alpha chains with 3,5-bis-dibromosalicyl-fumarate (HbXLDBBF) were infused into isolated perfused rabbit hearts. Vasoactivity and myocardial performance were determined using an isovolumic Langendorff preparation. With constant coronary flow, infusion of SFH (55 mg/dl) resulted in a 56% increase in aortic pressure as opposed to 29% and 11% increases with HbAo and HbXLDBBF, respectively. Rates of aortic pressure increase were over 6 times greater with SFH than with either HbAo or HbXLDBBF and exhibited concentration dependence only with SFH. Myocardial function remained normal. With constant coronary pressure, coronary flow decreased by 36% with SFH accompanied by a 23% decline in left ventricular developed pressure indicative of ischemia. With HbAo or HbXLDBBF, coronary flow decreased by only 1/3 that with SFH, while developed pressure declined by 4% and 2% with HbAo and HbXLDBBF, respectively. These data suggest that purification of HbAo to a single component can eliminate the dominant of at least two distinct vasoactive factors normally found in SFH. Furthermore, the physiological significance of the vascular response to HbAo is minimal. Preparations of HbXLDBBF accomplish this same result.","PeriodicalId":77498,"journal":{"name":"Biomaterials, artificial cells, and artificial organs","volume":"18 2","pages":"263-82"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199009117306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13519423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Use of cell culture to predict toxicity of solid materials in blood contact. 利用细胞培养预测血液接触中固体物质的毒性。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117314

A Wieslander, A Magnusson, P Kjellstrand

引用次数: 7

Ectopic bone induction by partially purified bone extract alone or attached to biomaterials. 部分纯化骨提取物单独或附着于生物材料诱导异位骨。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117316

H F Swoboda, F M Wimmer, K Pfeiffer, K H Schmidt

{"title":"Ectopic bone induction by partially purified bone extract alone or attached to biomaterials.","authors":"H F Swoboda, F M Wimmer, K Pfeiffer, K H Schmidt","doi":"10.3109/10731199009117316","DOIUrl":"https://doi.org/10.3109/10731199009117316","url":null,"abstract":"Formation of new bone is suggested to be caused by interaction of a set of chemical factors with mesenchymal target cells. A specific assembly of factors, i.e. chemotaxis-, adhesion-, proliferation- and differentiation-factors, as well as macromolecular structure components, essential for formation of large amounts of ectopic bone was termed \"osteopoetin\" (OP). In the present study a partially purified osteopoetin containing bovine bone extract (OCBE) was used to induce ectopic bone formation. In order to reduce the amount of OCBE necessary for bone induction, OCBE was seeded onto different commercially available collagens or poly-L-lactic acids (PL). Solid collagens in a sponge-like form were used for the first time to function as an attachment system for an osteoinductive substance. The test substances were implanted into abdominal muscle pouches of male Wistar rats. After 21 days the implants were harvested and evaluated histologically. OCBE resulted in the formation of large ossicles containing hematopoietic bone marrow. The minimal amount of OCBE to elicit ectopic bone formation can be reduced by a factor of 10 when attached to quickly resorbable collagens, but not when attached to slowly resorbable PL. These results suggest that collagens are suitable OCBE attachment systems and useful for clinical application.","PeriodicalId":77498,"journal":{"name":"Biomaterials, artificial cells, and artificial organs","volume":"18 3","pages":"383-401"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199009117316","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13533366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Is there a possibility of a bioartificial pancreas? 生物人工胰腺有可能吗?

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009119622

G Reach

引用次数: 1

Pathogenetic substantiation of extracorporeal detoxication in surgery. 手术中体外解毒的病理证实。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009119625

L A Ender, A M Lekhtman, A I Lobakov

引用次数: 0

Investigation of blood-biomaterial interaction by means of a new quantitative dynamic measuring principle. 用一种新的定量动态测量原理研究血液-生物材料相互作用。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009119627

T Groth, C Vassilieff, H Wolf, H Kuhn

引用次数: 0

Natural history of peritonitis post-peritoneal dialysis (PPPD). 腹膜透析后腹膜炎的自然病史。

Biomaterials, artificial cells, and artificial organs Pub Date : 1990-01-01 DOI: 10.3109/10731199009117294

A Treviño-Becerra, M M Gómez

引用次数: 0