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[Comparing studies on the differences of CCl4 liver injury and immunity liver injury mice models]. [CCl4肝损伤与免疫性肝损伤小鼠模型差异的比较研究]。
Shi yan sheng wu xue bao Pub Date : 2005-10-01
Yong Chen, Ming Cheng, Feng Mei Han, Peng Du
{"title":"[Comparing studies on the differences of CCl4 liver injury and immunity liver injury mice models].","authors":"Yong Chen,&nbsp;Ming Cheng,&nbsp;Feng Mei Han,&nbsp;Peng Du","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Comparison between CCl4 liver injury model and immunity liver injury model was studied through physiological, biochemical and gene expression profile methods, and the pathological mechanism of them were discussed. There are significant changes about the physiological, biochemical parameters including ALT,AST between two model groups and normal control group. Among the 14112 target genes, 379 and 293 differentially expressed genes were screened out from the both two model groups respectively in gene expression profile experiments, including 105 common, differentially expressed genes. The results indicate that the two models of CCl4 and immunity have some similarities. However, the differences between them are also obvious. It is useful for the further investigation on the liver-injury mechanism and pathological process of the two different liver-injury models.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 5","pages":"417-22"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25758257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Ultracytochemical localization of enzymes and DNA analysis during the development of garlic]. [大蒜发育过程中酶的超细胞化学定位和DNA分析]
Shi yan sheng wu xue bao Pub Date : 2005-10-01
Feng Ju Wei, Ying Ying Zhang, Chun Yan Hou, Sheng Fang Han, Dong Mei Wang
{"title":"[Ultracytochemical localization of enzymes and DNA analysis during the development of garlic].","authors":"Feng Ju Wei,&nbsp;Ying Ying Zhang,&nbsp;Chun Yan Hou,&nbsp;Sheng Fang Han,&nbsp;Dong Mei Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is believed that during storage, the parenchyma cells of garlic (Allium sativum L.) bulb would wither and fade gradually, and nutrients released inside the cells become available for the germination and growth of the young bud. In this study, The distributions of acid phosphatase (APase) and Adenosine Triphosphatase (ATPase) during germination were analyzed based on the method of lead precipitation at the electron microscopic level. It was found that their activities presenting in plasma membrane, cell wall and plasmodesma increased along the different developmental periods during storage. The fact that the most intensive enzymatic activity of APase and ATPase appeared at germination indicates that degradation, transformation and exportation of cell matrix are helpful in complete translocation of nutrient to new bud. The DNA in the degrading parenchyma cells was analyzed using the agarose electrophoresis. Results clearly showed a typical DNA ladder on the gel, indicating that gene-controlled, programmed cell death may contribute to the degradation of garlic parenchyma cells.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 5","pages":"387-96"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25758366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The character of calcium distribution in developing anther of lettuce (Lactuca sativa L.)]. [莴苣（Lactuca sativa L.）花药发育中的钙分布特征]。
Shi yan sheng wu xue bao Pub Date : 2005-10-01
Yi Lan Qiu, Ru Shi Liu, Chao Tian Xie, Yan Hong Yang, Qing Xu, Hui Qiao Tian
{"title":"[The character of calcium distribution in developing anther of lettuce (Lactuca sativa L.)].","authors":"Yi Lan Qiu, Ru Shi Liu, Chao Tian Xie, Yan Hong Yang, Qing Xu, Hui Qiao Tian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Potassium antimonite was used to locate calcium in the anther of lettuce (Lactuca sativa L) during its development. At the early stage of anther development there were few calcium granules in microspore mother cells and the cells of anther wall. After meiosis of microspore mother cells, calcium granules first appeared in the tapetal cells in which some small secretive vacuoles containing many calcium granules were formed and secreted into locule. Then, the tapetal cells began to degenerate. At the late stage of microspore, tapetal cells completely degenerated and its protoplast masses moved into anther locule with many calcium granules. Few calcium granules were precipitated in the microspores just being released from tetrad, but some on the surface of exine. Then calcium granules appeared in the nucleus and cytoplasm of early microspores, as wall as in the exine. When microspores formed some small vacuoles containing some calcium granules, and then the small vacuoles fused to form a large vacuole, the calcium granules in the nucleus and cytoplasm evidently decreased, microspore developed to the late stage. The result suggested that calcium is related to the formation of large vacuole in microspores. The wall of microspore also is a main location of calcium granules during its developing. At early microspore some calcium granules began to accumulate in exine, which suggested calcium related with exine formation. At late stage of microspore, most of calcium granules were mainly deposited on the surface of exine. After the first mitosis of microspores, the large vacuole of bicellular pollen disappeared and calcium granules in the large vacuole went back to cytoplasm again. When bicellular pollen synthesized starches some calcium granules appeared on the surface of starches, which suggested calcium may regulate starch synthesis. With amount of starches increasing, calcium granules disappeared from pollen cytoplasm and only some of them located on the surface of pollen.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 5","pages":"377-86"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25758365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Factors affecting liposome-mediated gene transfection of mouse somatic cells]. [影响脂质体介导小鼠体细胞基因转染的因素]。
Shi yan sheng wu xue bao Pub Date : 2005-10-01
Jian Ning Yu, De Qiang Miao, Suo Feng Ma, Xiu Wen Tan, Ji Hong Yuan, Jing He Tan
{"title":"[Factors affecting liposome-mediated gene transfection of mouse somatic cells].","authors":"Jian Ning Yu,&nbsp;De Qiang Miao,&nbsp;Suo Feng Ma,&nbsp;Xiu Wen Tan,&nbsp;Ji Hong Yuan,&nbsp;Jing He Tan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We studied the effects of the amount of liposome and plasmid, exposure time of cells to the liposome-plasmid complexes, number of cell passages and cell types on GFP gene transfection of mouse somatic cells. The maximal GFP transgene expression (30.7%) was achieved when mouse fetal fibroblast cells (MFFC) at 70%-90% confluence of passage 3 were exposed for 6 h to the complexes of 4 microg liposome (LipofectAMINE) and 0.3 microg plasmid (pEGFP-N1). Under these conditions, we compared the effect of the number (from primary to 15) of passages on the transfection efficiency of MFFC. The transfection efficiency of MFFC was 10.0%, 28.9% and 7.2% at the primary, 3rd and 15th passage, respectively, which indicated that the transfection efficiency decreased with passaging. When MFFC, mouse oviductal epithelial cells (MOEC) and mouse granulosa cells (MGC) were transfected at passage 3, the transfection efficiency was 27.8%, 13.7% and 14.2%, respectively, under the described transfection conditions. When the cell cycle stages of different cell types at transfection were examined, it was found that 17.2% of MFFC, 8.7% of MOEC and 9.9% of MGC were at M phases of the cell cycle. Examination of the cell cycle stages of MFFC at different passages showed that MFFC at the third passage had the highest percentage of M cells and the percentage decreased afterwards. This suggested that the transfection efficiency was correlated with the percentages of cells at M phase, and provided essential data for improvement of the transfection efficiency.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 5","pages":"404-10"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25758255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effect of glutamine on the expression of grp75 in PC12 cells]. 谷氨酰胺对PC12细胞grp75表达的影响
Shi yan sheng wu xue bao Pub Date : 2005-10-01
Wen Liu, Yan Zhang, Qing Feng Wu, Jin Yan Li, Ji Zuo
{"title":"[Effect of glutamine on the expression of grp75 in PC12 cells].","authors":"Wen Liu,&nbsp;Yan Zhang,&nbsp;Qing Feng Wu,&nbsp;Jin Yan Li,&nbsp;Ji Zuo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Glutamine (Gln), a conditionally essential amino acid, can be a potential enhancer of the heat stress response. And glucose-regulated protein 75(grp75) is a member of the hsp family. To evaluate the effect of glutamine on the expression of grp75, PC12 cell was cultured with DMEM, glucose-free DMEM, DMEM within Gln, and glucose-free DMEM within Gln, and the expression of grp75 was detected by immunocytochemistry and western blot, the mRNA level of grp75 in the cells was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Data indicated that Gln can upregulated the expression of grp75 in PC12 cell line, and the effect is more significantly in PC12 cell which was glucose deprivation than the normal cell. To investigate the effect of Gln on PC12 cells under glucose deprivation, MTT method was used to monitor the cell viability after Gln treatment for the cell under glucose depriving. The experiments results showed that glutamine at a concentration range of 0.2-40 mmol/L significantly enhanced the cell viability in glucose-free DMEM. And the protective effect to grp75 low-expresssion on PC12 cells is markedly decreased.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 5","pages":"423-31"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25758258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Spontaneous and induced programmed cell death in suspension cell cultures of cotton (Gossypium hirsutum L.)]. 棉花(Gossypium hirsutum L.)悬浮细胞培养的自发和诱导程序性细胞死亡[j]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Qi Zhong Xia, Xian Long Zhang, Yi Chun Nie, Xiao Ping Guo, Long Fu Zhu
{"title":"[Spontaneous and induced programmed cell death in suspension cell cultures of cotton (Gossypium hirsutum L.)].","authors":"Qi Zhong Xia,&nbsp;Xian Long Zhang,&nbsp;Yi Chun Nie,&nbsp;Xiao Ping Guo,&nbsp;Long Fu Zhu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cotton suspension cells grew well in the MS medium supplemented with 0.1 mg/L 2,4 D and 0.1 mg/L KT. Senescence occurred when the cells were unsubcultured. The cells began to lose their viabilities on the 17th day, and on the 21th day oligonucleosomal sized DNA fragments ( DNA ladder) could be detected. Oligonucleosomal sized DNA fragments ( DNA ladder) was the hallmark of the programmed cell death. Programmed cell death of cotton suspension cells could be induced respectively by some stress factors which included heatshock (42+/-3 degrees C for 8 hours), 10 micromol/L camptothecin, 20 micromol/L fumonisin B1 and 50 mmol/L cycloheximide. The cotton suspension cells which grew in the MS medium supplemented with 0.1 mg/L 2,4 D and 0.1 mg/L KT differred physiologically from the cells in the MS medium supplemented with 0.1 mg/L IBA and 0.1 mg/L KT, and they responded differentially to the heatshock, 10 micromol/L camptothecin and 20 micromol/L fumonisin B1, while the same to 50 mmol/L cycloheximide.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"303-8"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effects of MG132 , an inhibitor of proteasome , on the pollen germination and tube growth of Pecea wilsonii]. [蛋白酶体抑制剂MG132对山核桃花粉萌发和管状生长的影响]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Xian Yong Sheng, Zheng Hai Hu
{"title":"[Effects of MG132 , an inhibitor of proteasome , on the pollen germination and tube growth of Pecea wilsonii].","authors":"Xian Yong Sheng,&nbsp;Zheng Hai Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ubiquitin/proteasome system is regarded as a major pathway of proteolysis in eukaryotic cells, in which the proteasome acts as primary protease for its function of degrading substrate proteins to short peptides. In the present paper, cytological, statistical studies and Fourier transform infrared (FTIR) analysis on the effects of MG132, an inhibitor of proteasome, on the pollen germination and tube growth of Pecea wilsonii were carried out in an artificial experimental system. It is showed that MG132 significantly reduced the germination rate and tube growth. Furthermore, MG132 treatment lead to vacuolization occurred both in tube cytoplasm and generative cell. While DMSO and non-proteasome inhibitor E-64 do not have similar effects. FTIR analysis revealed that MG132 treatment markedly reduced the contents of wall-bound proteins and pectin at the apex of tube. Those findings provided evidence that by inhibiting the activity of proteasme, MG132 strongly affects pollen germination and tube growth of P. wilsonii, and that UPP plays an important role in organization and maintaining polarized growth of pollen tube. Inhibition of UPP will induce apoptosis of pollen tube.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"309-16"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Genetic diversity of yam (Dioscorea opposita Thunb) detected by ISSR markers]. [利用ISSR标记检测山药(Dioscorea opposita Thunb)的遗传多样性]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Yan Qing Zhou, Jian Zhou Jing, Zhen Yong Li, Jian Hao, Jing Fen Jia, Bao Hua Zhang, Jian Guo Hao
{"title":"[Genetic diversity of yam (Dioscorea opposita Thunb) detected by ISSR markers].","authors":"Yan Qing Zhou,&nbsp;Jian Zhou Jing,&nbsp;Zhen Yong Li,&nbsp;Jian Hao,&nbsp;Jing Fen Jia,&nbsp;Bao Hua Zhang,&nbsp;Jian Guo Hao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genetic diversity of 28 cultivars of yam (Dioscorea opposita Thunb) was assessed by means of Inter-simple sequence repeat (ISSR) markers. The results showed that seven proper primers, with rich polymorphism, could be selected from a total of forty four ISSR ones; distinct differences appeared among 28 cultivars amplified bands, and the rate of polymorphic bands was 83.01%; Shannon's Information index was 0.3191; a Jaccard's genetic similarity matrix and a dendrogram for these cultivars were formed, in which they could be divided into four groups: Group 1 was composed of D. opposita. cv. Ribenbai, D. opposita. cv. Huashanyao and D. opposita. cv. Ribenyuan; Group2 contained D. opposita. cv. Xiaoye; Group 3 contained D. opposita. cv. No.1 Songye; other 23 cultivars were put into Group4. PCA(Principal component analysis) was employed to evaluate the resolving power of the markers to differentiate among them. This laid the foundation of the identification of yam cultivars and the efficient use of its germplasm resources.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"324-30"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Relationship of microtubule and [Ca2+]cyt in wheat mesophyll protoplast]. [小麦叶肉原生质体微管与[Ca2+]细胞的关系]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Gang Liu, Dong Mei Wang
{"title":"[Relationship of microtubule and [Ca2+]cyt in wheat mesophyll protoplast].","authors":"Gang Liu,&nbsp;Dong Mei Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By using immunofluorescence labeling technique and incubating the Fluo-3AM of Ca2+ fluorescein, wheat (Triticum aestivum) cultivars Lovrin 10 was selected as mesophyll protoplast material in this paper for studying the relationship between the pattern of microtubule and [Ca2+]cyt. Laser scanning confocal microscopy(LSCM) was used and pharmacologic examination was set. The results indicated there was a connection between calcium signaling and microtubule organization in wheat mesophyll protoplast. When the cytosolic calcium concentration distinctly ascends, the pattern of microtubule showed depolymerization; Depolymerization of microtubule could enhance extracellar calcium afflux.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"331-9"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Calcium involved in the signaling pathway of jasmonic acid induced stomatal closure of Vicia faba L]. [钙参与茉莉酸诱导蚕豆气孔关闭的信号通路]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Xin Liu, Wu Liang Shi, Shu Qiu Zhang, Cheng Hou Lou
{"title":"[Calcium involved in the signaling pathway of jasmonic acid induced stomatal closure of Vicia faba L].","authors":"Xin Liu,&nbsp;Wu Liang Shi,&nbsp;Shu Qiu Zhang,&nbsp;Cheng Hou Lou","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ca2+, an ubiquitous second messenger in the signal transudation pathway, is required for various physiological and developmental processes in plant. Jasmonic acid (JA) has been known to induce the stomatal closure. By monitoring the changes of [Ca2+]cyt with fluorescent probe Fluo-3 AM under the confocal microscopy, we observed that exogenous JA increased [Ca2+]cyt in guard cells of Vicia faba L. while the control and linolenic acid (LA), which is a precursor of JA, could hardly affect the change of [Ca2+]cyt. EGTA, a chelator of Ca2+ completely blocked JA-induced stomatal closure. After epidermis pretreated with EGTA, JA failed to result in [Ca2+]cyt increasing. Ruthenium red that blocked Ca2+ released from intracellular Ca2+ store could not significantly change JA-induced stomatal closure, while JA still increased [Ca2+]cyt. Furthermore, Ca2+ channel inhibitor of nifedipine (NIF) reduced the effectiveness of JA-induced stomatal closure and JA-induced increasing fluorescent intensity in guard cells. The results demonstrated that Ca2+ is involved in the signal transduction of JA induced stomatal closure, and the source of [Ca2+]cyt increasing in guard cells induced by JA might derive mainly from the external stores.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"297-302"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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