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[The expression of vegf in rat fracture reparative callus]. vegf在大鼠骨折修复性愈伤组织中的表达。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Chang You Li, De Yong Liang, Guang Yu Fan, Jin Dan Song
{"title":"[The expression of vegf in rat fracture reparative callus].","authors":"Chang You Li,&nbsp;De Yong Liang,&nbsp;Guang Yu Fan,&nbsp;Jin Dan Song","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study has determined the histological changes, VEGF (Vascular endothelial growth factor) mRNA alternative expressions and VEGF mRNA levels in chondrogenic-callus, osteogenic-callus and total callus by histology, RT-PCR and Northern blot during rat fracture repair. The results showed that the cellular events of intramembranous ossification, chodrogenesis and endochondral ossification appeared in the callus simultaneously and sequentially. The VEGF mRNAs were expressed in the osteogenic-callus and the chondrogenic-callus, and the strongest expressions were VEGF120 and VEGF164 mRNA. The VEGF mRNA levels in the total callus increased with time after fracture. Take together, these findings suggest that osteogenesis-derived VEGF may play a potential role to modulate the differentiation process of osteogenic and chondrogenic cells during fracture repair.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"359-62"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The dynamics of calcium distribution in stigma and style of lettuce (Lactuca sativa L.) before and after pollination]. [授粉前后钙在莴苣（Lactuca sativa L. ）柱头和花柱中的分布动态]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Yi Lan Qiu, Ru Shi Liu, Chao Tian Xie, Yan Hong Yang, Li Gu, Hui Qiao Tian
{"title":"[The dynamics of calcium distribution in stigma and style of lettuce (Lactuca sativa L.) before and after pollination].","authors":"Yi Lan Qiu, Ru Shi Liu, Chao Tian Xie, Yan Hong Yang, Li Gu, Hui Qiao Tian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Potassium antimonite was used to deposit calcium in the stigma and style of lettuce (Lactuca sativa L.) before and after pollination. The stigma of lettuce is two splits. Abundant calcium granules are displayed in the wall of papillae on the receptive surface of stigma before and after pollination, which may facilitate pollen germination. However, a few calcium granules in the wall of epidermis cell on no-receptive surface. Calcium distribution in style presents a gradient in transmitting tissue and parenchyma cells from the top to the base of the style before pollination. After pollination, calcium in transmitting tissue distinctly increased and its gradient distribution became more evident. Pollen tubes grow in the intercellular gaps of transmitting tissue. When pollen tubes grew into transmitting tissue, calcium granules in parenchyma around transmitting tissue decreased, suggesting a calcium movement was controlled by pollen tubes. The calcium gradient distribution also appeared in the trachea of vascular bundle of style. In general, calcium in style displays a feature of time-special distribution: transmitting tissue doesn't need much more calcium that is only stored in the parenchyma before pollination. However, calcium in parenchyma cells may be transported to transmitting tissue and make the latter contain more calcium to form an evident calcium gradient and meet the requirement of pollen tubes directionally growing after pollination. This is the second sample of calcium gradient existing in style, which was found by using potassium antimonite method.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"277-86"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effect of RhoA and cAMP on morphological changes in human prostate cancer cell line PC-3]. RhoA和cAMP对人前列腺癌细胞PC-3形态学变化的影响。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Yong Chang Chen, Ying Wang, Hao Yu, Wen Rong Xu
{"title":"[Effect of RhoA and cAMP on morphological changes in human prostate cancer cell line PC-3].","authors":"Yong Chang Chen,&nbsp;Ying Wang,&nbsp;Hao Yu,&nbsp;Wen Rong Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper was designed to investigate the effect of RhoA and cAMP/PKA mediating signal transduction on the morphological changes of human prostate cancer cell line PC-3. Different RhoA cDNA expressing constructs were used to transfect PC-3 cells. Transfected and untransfected cells were stimulated with lysophosphatidic acid(LPA) and/or cAMP. The morphological changes of the cells were recorded by inversive microscope. Results showed that the untransfected cells changed their conformation from polygonal to round with the stimulation of LPA,while cAMP prevented the change. LPA caused the same change in cells transfected with wild type, constitutively active type and 188 mutant of RhoA. cAMP prevented the change in cells transfected with wild type and constitutively active RhoA but not in cells transfected with 188 mutant of RhoA. The results suggested that cAMP/PKA could inhibit the morphological effect of RhoA through phosphorylating RhoA on 188 serine.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"363-7"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells]. [乙酰胆碱和阿托品对人SK-N-SH细胞增殖分化及mAchR1表达的影响]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Xin Hu, Jian Xin Lu
{"title":"[The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells].","authors":"Xin Hu,&nbsp;Jian Xin Lu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The SK-N-SH human neuroblastoma cells were treated with acetylcholine (Ach) and atropine (Atro), the proliferation activity of cells was detected by CCK-8 method and the cell cycle distribution was determined by flow cytometry. The changes of the mRNA and protein of mAchR1 and c-fos were analysed by fluorescence quantitative PCR, western blot and flow cytometry indirect-immunofluorescence. Results showed 1 mmol/L Ach could significantly stimulate the proliferation of SK-N-SH cells and 1 mmol/L Atro could prevent SK-N-SH cells from S phase growing into G2/M phase; 1 mmol/L Ach and 1 mmol/L Atro had feedback adjustment on the protein level of mAchR1, but had no significant effect on mRNA level of mAchR1. 1 mmol/L Ach increased the both levels of mRNA and protein of Fos obviously, this effect was reversed by Atro. All phenomena clue us cholinergic receptors joining the events of cell proliferation.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"287-96"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The change of microtubule organization influencing cell ability for fusion]. [影响细胞融合能力的微管组织变化]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Li Meng, Xiong Bo Peng, Meng Xiang Sun
{"title":"[The change of microtubule organization influencing cell ability for fusion].","authors":"Li Meng, Xiong Bo Peng, Meng Xiang Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is well known that plants have ability to avoid polygamy in angiosperm, however, its mechanism is still poorly understood. In our previous work of in vitro fertilization we found that once an egg cell fused with a sperm cell, it could hardly fuse with other sperm within a certain period. Therefore we proposed that fusing egg cell must suffer some intrinsic changes during the fusion, such as the change of microtubule organization, which may prevent the egg cell from another fusion with extra sperm cells. In present work, the dynamic change of microtubule organization during the process of cell fusion in Nicotiana tabacum was observed through the single cell immunofluorescence microscopy. It was found that once cells began to adhere and fuse in Polyethylene Glycol (PEG), the microtubule immediately depolymerized. During this period of fusion and before microtubule polymerized again in the fusion product, the cell was unable to fuse with other cells. The results suggested microtubule organization was likely involved in the mechanisms of controlling cell fusion, and offered a clear indication that similar mechanism might also contribute to plant avoiding polygamy.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"347-52"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The study on the STAT3 labeled by GFP affecting the migration of BEL-7402 cell line]. [GFP标记STAT3对BEL-7402细胞系迁移影响的研究]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Run Lin Yang, Fei Yan Pan, Jun Yuan, Shi Ying Guo, Hong Xia Zhang, Dong Hong Zhao, Chao Jun Li
{"title":"[The study on the STAT3 labeled by GFP affecting the migration of BEL-7402 cell line].","authors":"Run Lin Yang,&nbsp;Fei Yan Pan,&nbsp;Jun Yuan,&nbsp;Shi Ying Guo,&nbsp;Hong Xia Zhang,&nbsp;Dong Hong Zhao,&nbsp;Chao Jun Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Signal transducers and activators of transcription (STATs) are key DNA-binding proteins in JAK/STATs signal pathway. Aberrant expression and activation of STAT3 have been identified in many kinds of tumors. We report here that constitutive activation of STAT3 was present in BEL-7402 cells. We constructed the fusing genes of STAT3 (wild type/mutant) and GFP to study the function of constitutively activated STAT3 in BEL-7402 cells. By measuring the migration of the cells labeled by GFP-STAT3(WT/CYF), we proved that overexpression of STAT3(WT) could augment the migration of BEL-7402 cells, while STAT3(CYF) could decrease the migration.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"317-23"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Culture, purification and biological characters of olfactory ensheathing cells from adult transgenic mice]. [成年转基因小鼠嗅鞘细胞的培养、纯化及生物学特性研究]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Chun Ting Wang, Shi Hua He, Hao Yang, Ping Zhang, Ling Ling Fei, Si Wei You, Gong Ju
{"title":"[Culture, purification and biological characters of olfactory ensheathing cells from adult transgenic mice].","authors":"Chun Ting Wang,&nbsp;Shi Hua He,&nbsp;Hao Yang,&nbsp;Ping Zhang,&nbsp;Ling Ling Fei,&nbsp;Si Wei You,&nbsp;Gong Ju","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Several studies have demonstrated the potential of olfactory ensheathing cells (OECs) for the repair of central and peripheral nerve injury. In this work, ensheathing cells that express the receptor gene coding for enhanced green fluorescent protein (eGFP) from adult mice were isolated and purified, and then, its biological characters were examined in vitro. The work was based on combinations of fluorescence confocal, phase contrast, cell proliferation assay and immunolabeling identification. The results showed that (1) two major morphologically and immunohistochemically distinct types of cells were present after 15 days in the primary cultures of adult transgenic mice olfactory nerves and glomerular layers of the olfactory bulb. One cell type was bipolar or multipolar OECs and strained positively for P75 low affinity neurotrophic receptor (P75N), S100, and glial fibrillary acidic protein(GFAP). The other type was fibroblasts with flat or endothelial-like shape, and reacted with antibody against Thy1.1. (2) A simple, inexpensive method for purifying ensheathing cells, in which various harvested cell types showed different rates of attachment to the uncoated culture ware, was developed. This technique neither binds any antibodies nor requires any costly equipment, and yields a large number of highly purified cells. (3) In sequential observations over 22 days in culture, the population of purified cells was maintained and continued to proliferate for longer. This experiment not only supported and advanced the ensheathing cell research but also offered ideal materials of in vivo transplantation for the repair of CNS injury.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"340-6"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Regulatory volume decrease and its mechanism in nasopharyngeal epithelial cells]. [鼻咽上皮细胞调节体积减少及其机制]。
Shi yan sheng wu xue bao Pub Date : 2005-08-01
Xue Rong Sun, Li Xin Chen, Jian Wen Mao, Lin Yan Zhu, Si Huai Nie, Ping Zhong, Pan Li, Li Wei Wang
{"title":"[Regulatory volume decrease and its mechanism in nasopharyngeal epithelial cells].","authors":"Xue Rong Sun,&nbsp;Li Xin Chen,&nbsp;Jian Wen Mao,&nbsp;Lin Yan Zhu,&nbsp;Si Huai Nie,&nbsp;Ping Zhong,&nbsp;Pan Li,&nbsp;Li Wei Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To investigate regulatory volume decrease (RVD) and its mechanism in primary-culturing fetal human nasopharyngeal epithelial cells, living cell imaging technique was employed to detect the volume changes following exposure to hypotonic solution, and blockage of Cl- channels was used to clarify the role of Cl- channels in RVD. The results showed that extracellular hypotonic treatment swelled the cells and induced RVD. 47% hypotonic solution (160 mOsmol/L) swelled the cell by 144.7% and induced 38.7% recovery of cell volume within 20 min. RVD was correlated negatively to the extracellular osmolarity (r=-0.99, P<0.05) and positively to the swelling volume(r=0.99, P<0.05) in \"S\" shape, respectively. Chloride channel blockers, tamoxfen (20 micromol/ L), ATP (10 mmol/L) and NPPB (100 micromol/L), inhibited RVD by 100%, 76.3% and 62.7% (P< 0.01), respectively. The results indicated that primary-culturing fetal human nasopharyngeal epithelial cells are capable of RVD. Cl- efflux through Cl- channels is the key mechanism of RVD.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 4","pages":"353-8"},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25641091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effective cryopreservation of human embryonic stem cells by programmed freezing]. 人类胚胎干细胞程序化冷冻的有效低温保存。
Shi yan sheng wu xue bao Pub Date : 2005-06-01
Peng Fei Yang, Hsiao Chien Tsung, Qi Kang Cheng, Tse Chao Hua, Chun Fang Wu, Yi Lin Cao
{"title":"[Effective cryopreservation of human embryonic stem cells by programmed freezing].","authors":"Peng Fei Yang,&nbsp;Hsiao Chien Tsung,&nbsp;Qi Kang Cheng,&nbsp;Tse Chao Hua,&nbsp;Chun Fang Wu,&nbsp;Yi Lin Cao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cryopreservation of human embryonic stem cells is an important and unsolved problem. A computer-controlled programmable cooler was used in the preservation of ES cells. Several effects have been experimentally studied, which include the cooling rates, the temperature of seeding, the temperatures before the samples being plunged into liquid nitrogen, and the cryoprotective agents. It was found that the favorable constitution of cryoprotective agents was Me2SO+ FBS+DMEM(1:3:6, v/v/v) with cooling protocol of -0.5 degrees C/min from 0 degrees C to -35 degrees C (seeding at -10 degrees C), and being plunged into the liquid nitrogen immediately. The high survival rate (81.8%) was obtained.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 3","pages":"247-56"},"PeriodicalIF":0.0,"publicationDate":"2005-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25212045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Mapping of apple Co gene using SSR markers]. 利用SSR标记定位苹果Co基因。
Shi yan sheng wu xue bao Pub Date : 2005-06-01
Yi Ke Tian, Cai Hong Wang, Hong Yi Dai
{"title":"[Mapping of apple Co gene using SSR markers].","authors":"Yi Ke Tian,&nbsp;Cai Hong Wang,&nbsp;Hong Yi Dai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Columnar apple is an important genetic resource for tree form breeding of apple. In this study, 106 individuals of the F1 population derived from 'Spur Fuji' (coco)x 'Telamon'(Coco) were used as plant materials for screening SSR markers linked to gene. By bulked segregating analysis (BSA), eight SSR markers from the tenth linkage group of apple genome were tested. Finally, three of them, COL, CH02a10 and CH03d11, were identified to be SSR markers of Co gene. Linkage analysis showed that the genetic distance of COL, CH02a10 and CH03d11 to Co locus was 15.3cM, 22.2cM and 3.9 cM, respectively. On the linkage map of these markers, Co gene was located between COL and CH03d11.</p>","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 3","pages":"272-5"},"PeriodicalIF":0.0,"publicationDate":"2005-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25212048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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