Shi yan sheng wu xue bao最新文献

[Hyperplasia and aggregation of mitochondrion in Pisum sativum leaf cells infected with BBWV 2]. [感染 BBWV 2 的豌豆叶细胞线粒体增生和聚集]。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Jian Hong, Wei Bing Wang, Xue Ping Zhou

{"title":"[Hyperplasia and aggregation of mitochondrion in Pisum sativum leaf cells infected with BBWV 2].","authors":"Jian Hong, Wei Bing Wang, Xue Ping Zhou","doi":"","DOIUrl":"","url":null,"abstract":"The alteration of mitochondrion in Pisum sativum leaf cells infected with B935,isolates of BBWV 2, were investigated by electron microscopy, immunogold-labeling technique and stereologic quantity. The results showed that mitochondria were hyperplastic and aggregated nearby the membranous proliferation in cytoplasm. Many mitochondria were enlarged and distorted. There are some square paracrystalline inclusions within abnormal mitochondria. The difference between infected and healthy leaf cells at mitochondrion was observed to reveal significances in volume density (V(V)), surface density (S(V)), numerical density (Nv)(P < 0.01), but it was varied in form factor (PE), circumference index (CI), ratio of surface to volume (R(SV)). Some immunogold particles were localized to the crystal substance between the mitochondria. A high density of immunogold particles were localized to the crystalline aggregates and tubules composed of virus particles. This indicated that hyperplasia and aggregation of mitochondrion related to the assembly of virion in infection cells. BBWV 2 particles were assembled at the position of adherent mitochondria. The paracrystalline inclusions within mitochondria could represent the accumulation of a certain protein.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 6","pages":"527-35"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25803271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Study on identification, colonization and reorganization of rice endophytic bacteria]. 水稻内生细菌的鉴定、定植与重组研究

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Ai Ping Zheng, Hui Qing Sun, Ping Li, Fu Rong Tan, Xiu Li Zheng, Zhuang Li

引用次数: 0

[An anti-tumor research of recombinant phage vaccine expressed xenogenic EGFR]. 表达外源EGFR的重组噬菌体疫苗抗肿瘤研究

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Xian Zhuo Zeng, Dong Liu, Liang Tang, Cai Cun Zhou, Li Song Tan, Xin Hua Cai, Xiao Wen Li, Jun Nian Zhou, Pei De Zhang, Shang Quan Zhang

{"title":"[An anti-tumor research of recombinant phage vaccine expressed xenogenic EGFR].","authors":"Xian Zhuo Zeng, Dong Liu, Liang Tang, Cai Cun Zhou, Li Song Tan, Xin Hua Cai, Xiao Wen Li, Jun Nian Zhou, Pei De Zhang, Shang Quan Zhang","doi":"","DOIUrl":"","url":null,"abstract":"A recombinant phage vaccine expressing EGFR on it's capsid was constructed and used to study the anti-tumor effect. The T7 phage display system was applied to display seven xenogenic (human, chicken) epidermal growth factor receptor extracellular domain fragments. The EGFR fragment was expressed as fused protein with 10B capsid on the surface of T7 phage. The T7-EGFR phage vaccines were injected into C57BL/6J mice, and then Lewis lung cancer cells were inoculated after 4 weeks immunization. The tumor tissue was excised and weighed after 10 days to evaluate the anti-tumor effect of each experimental group. The EGFR expression of the phage vaccine was verified by western-blot analysis. The A431 cells with high expressed EGFR was used to detect the anti-EGFR antibody by flow cytometry analysis. The results showed that the A431 cell can react with the serum obtained from the mice after three-week immunization. The experimental results confirmed that special EGFR antibody could be induced by the T7-EGFR phage vaccine. The T7-EGFR phage vaccine can elicit endogenous special EGFR antibody in mice and is capable of suppressing the tumor proliferation and retarding the growth of Lewis lung cancer. This research can be used to develop an anti-tumor vaccine for the target-therapy of EGFR(+) tumor.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 6","pages":"481-9"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25803976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The ultrastructural observation of anthers of Chinese cabbage's mail-sterility]. 白菜不育花药的超微结构观察。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Chao Tian Xie, Yan Hong Yang, Li Li Ge, Rui Wang, Hui Qiao Tian

{"title":"[The ultrastructural observation of anthers of Chinese cabbage's mail-sterility].","authors":"Chao Tian Xie, Yan Hong Yang, Li Li Ge, Rui Wang, Hui Qiao Tian","doi":"","DOIUrl":"","url":null,"abstract":"The fertile and sterile anthers of a Chinese cabbage (Brassica campestris L. ssp. chinensis Makino) were observed using electron microscope to find the ultrastructural feature of sterile anthers. The earliest abnormal phenomenon in sterile anther was nucleolus of sporogenous cells locating in the edge of nucleus. During microspore mother cell development, callus wall surrounding the cell displayed uneven in the thick ness and was discontinuous,and the some cytoplasm leaked out of the cell from some rifts in the wall. After meiosis of microspore mother cells, the cells of tetrad were irregular and some of them contained several nuclei. The exine of pollen began to be formed in tetrad in this cabbage. The evident disorder during exine formation in the sterile pollen occurred during its primexine formation and then the sporopollenin was irregularly deposited to form a layer of uneven and discontinuous pollen exine. Cytoplasm of aborting microspores contracted and finally degenerated after them released from tetrad. The tapetal cells of fertile anther began to synthesis abundant lipid material during microspore development. However, the tapetal cells of sterile anther did not synthesis lipid material during microspore aborting. The microspore abortion was first and tapetal degeneration second. Therefore, aborting microspore induced the functional default of tapetal cells synthesizing lipid material. The ultrastructural results on this study further complete and correct our previous results obtained by light microscope.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 6","pages":"501-12"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25803268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Cytokinin changes of root exudates, leaves and grains in inter-subspecific hybrid rice]. [亚种间杂交稻根系分泌物、叶片和籽粒细胞分裂素的变化]。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Bo Shen, Qin Hong Pan, Long Xing Tao, Xi Wang

引用次数: 0

[Prokaryotic expression, antiserum preparation and cytochemical analysis of TaMlo3 in wheat]. 小麦中TaMlo3的原核表达、抗血清制备及细胞化学分析。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Fan Li, Dong Wei Hu, Feng Chen, Xiao Feng Cui

引用次数: 0

Study on total cell antioxidant capacity in Leber's hereditary optic neuropathy. Leber遗传性视神经病变细胞总抗氧化能力的研究。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Jian Yong Wang, Hui Yan Zhang, Yu Min Li, Hai Ning Yu, Jing Wang, Sheng Rong Shen, Yi Tong

引用次数: 0

[sdt1, a novel Arabidopsis mutant tolerant to drought and high-salinity stresses]. [sdt1，一种耐干旱和高盐胁迫的拟南芥突变体]。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Zhi Miao Li, Cheng Shi Yan, Gang Wu, Jia Shu Cao, Zu Hua He

引用次数: 0

[Expression patterns of GCN5 and HDAC1 in preimplantational mouse embryos and effects of in-vitro cultures on their expressions]. [GCN5和HDAC1在小鼠着床前胚胎中的表达模式及体外培养对其表达的影响]。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Dong Mei Zhao, Chen Ming Xu, He Feng Huang, Yu Li Qian, Fan Jin

{"title":"[Expression patterns of GCN5 and HDAC1 in preimplantational mouse embryos and effects of in-vitro cultures on their expressions].","authors":"Dong Mei Zhao, Chen Ming Xu, He Feng Huang, Yu Li Qian, Fan Jin","doi":"","DOIUrl":"","url":null,"abstract":"To investigate the expression patterns of histone acetyltransferase (GCN5) and histone deacetylase 1 (HDAC1) in preimplantation mouse embryos and the effects of in-vitro cultures on their expressions, immunocytochemistry was used to detect the expressions of GCN5 and HDAC1 in mouse embryos at the stages of two-cell, four-cell, eight-cell, morula and blastocyst in both in-vivo and invitro groups. In in-vivo group, the obvious expressions of GCN5 were observed in the cytoplasm of all kinds of mouse embryos but blastocysts. Meanwhile, HDAC1 was highly expressed in the nuclei of four and eight-cell embryos and morula but mainly seen in the cytoplasm of two-cell embryos. In blastocysts, the HDAC1 fluorescence was limited to the nuclei of trophoblast cells. In in-vitro group, there were no obvious GCN5 expressions in all kinds of embryos and the expression of HDAC1 was significantly reduced although its expression pattern was similar to that of in-vivo group. Those results showed that in -vitro culture environments could inhibit GCN5 expression and decrease the expression of HDAC1 in mouse preimplantation embryos, which might affect the correct embryonic gene expression.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"38 6","pages":"513-9"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25803269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Studies on the megasporogenesis and microsporogenesis and the development of their female and male gametophyte in Magnolia biloba]. [木兰大孢子发生和小孢子发生及其雌、雄配子体发育的研究]。

Shi yan sheng wu xue bao Pub Date : 2005-12-01

Li Lin Wang, Jiang Qin Hu, Ji Liang Pang, Tai He Xiang

引用次数: 0